Predicted mutation
evidence seq id position mutation annotation gene description
RA pSIJ663_pHT315_araE_xy 5,205 G→A intergenic (+474/–) ampR → / – –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*pSIJ663_pHT315_araE_xy5,2050GA98.3% 59973.2 / NA 18121intergenic (+474/–)ampR/––/–
Reads supporting (aligned to +/- strand):  ref base G (6/24);  major base A (8526/9224);  minor base C (20/287);  total (8568/9553)
Fisher's exact test for biased strand distribution p-value = 0.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 18240 total aligned reads displayed.

GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGC  >  pSIJ663_pHT315_araE_xy/5056‑5352
                                                                                                                                                     |                                                                                                                                                   
    aGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGGCGTGTCTTACCGGGTTGGACTCAAGACGATAGGTACCGGGTGAGGCACAGc                                                                                                                                                 >  2:4051921/1‑150 (MQ=255)
       cGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGt                                                                                                                                              >  1:1078472/1‑150 (MQ=255)
         tAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGCTACCAGTGGCTGCTGCCAGGGGCGATAAGTCGAGGCTTACCGGGTTGGACTCAAGACGGAAGTTACCGGTTGAGGCACAGCGGTCg                                                                                                                                            >  1:3541145/1‑150 (MQ=255)
           gTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCggg                                                                                                                                          >  2:1976313/1‑150 (MQ=255)
            ttAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGc                                                                                                                                         >  1:2140616/1‑150 (MQ=255)
             tAGGCCACCCCTTCAAGAACTCTGTAGCCCCGCCTACATCCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCt                                                                                                                                        <  2:1127135/150‑1 (MQ=255)
              aGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTg                                                                                                                                       >  1:1823345/1‑150 (MQ=255)
              aGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGGCGGGCTg                                                                                                                                       >  1:2471214/1‑150 (MQ=255)
              aGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCCAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTg                                                                                                                                       <  2:534302/150‑1 (MQ=255)
                gCCACCACTTCAAGAACTCTGTAGCACCCCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGaa                                                                                                                                     <  2:2936993/150‑1 (MQ=255)
                 ccaccaCTTCAAGAACGCTGTAGCCCCGCCTACATACGTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAAc                                                                                                                                    <  2:3785176/150‑1 (MQ=255)
                 ccaccaCTTCAAGAACCCTGTAGCCCCGCCTACATTCCTCGCTCGGCTAATCCTGTTACCAGTGGTTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAAc                                                                                                                                    <  2:4387270/150‑1 (MQ=255)
                  caccacTTCAAGGACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGGTACCTGGGGCTGCTGCCAGTGCCGAAAAGTCGTGGCATACCGGGTTGGACTCCAGACGATAGTTACCGGACAAGGCACACcgggcggggaggacg                                                                                                                                   >  2:3234079/1‑143 (MQ=255)
                  caccacTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGGACg                                                                                                                                   >  1:3707406/1‑150 (MQ=255)
                     cacTTCAAGAACTCTGTAGCACCGCCTACATTCCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTTAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACgggg                                                                                                                                >  1:2391383/1‑150 (MQ=255)
                          cAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTc                                                                                                                           >  1:2225446/1‑150 (MQ=255)
                           aaGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAGGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCg                                                                                                                          >  2:1889310/1‑150 (MQ=255)
                             gAACCCTGTAGCACCGCCTACATACCTCGCTCTGCTAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTg                                                                                                                        <  1:878613/150‑1 (MQ=255)
                              aaCTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGc                                                                                                                       >  2:2436034/1‑150 (MQ=255)
                                   tGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGcacaca                                                                                                                  <  2:2169671/150‑1 (MQ=255)
                                       gCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCACTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACCGGGGGTTCGTGCACACAGccc                                                                                                              >  1:1325212/1‑150 (MQ=255)
                                        cACCGCCTCCATCCCTCGCTCTGCTAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCa                                                                                                             <  2:1366005/150‑1 (MQ=255)
                                            gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCtt                                                                                                         <  2:3599645/150‑1 (MQ=255)
                                            gCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGGGGCGATAAGTCGGGTCTTACCGGGTTGAACTCAAGTCGATATTTACCGGATAAGGCACAGGGGTCGGGCTGGACGGGGGGTCCGGGCCCACAGCCCAGCtt                                                                                                         >  2:4573326/1‑150 (MQ=255)
                                             ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGGACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                                        >  1:1749012/1‑150 (MQ=255)
                                             ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                                        >  1:419280/1‑150 (MQ=255)
                                             ccTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTg                                                                                                        >  1:1399132/1‑150 (MQ=255)
                                                aCATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGGTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAg                                                                                                     >  2:725522/1‑150 (MQ=255)
                                                 cATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGc                                                                                                    <  2:3951429/150‑1 (MQ=255)
                                                  ctACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGCCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTAGCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCg                                                                                                   <  1:451982/149‑1 (MQ=255)
                                                  aTACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCg                                                                                                   <  1:2791249/150‑1 (MQ=255)
                                                   tACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGGCTCAAGCCGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGGACGGGGGGGTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                                  >  1:116349/1‑150 (MQ=255)
                                                   tACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGa                                                                                                  <  1:3378026/150‑1 (MQ=255)
                                                     ccccGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc                                                                                                <  2:1043648/147‑1 (MQ=255)
                                                     ccTCGCTCTGCTAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAGCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAAc                                                                                                <  2:4226999/150‑1 (MQ=255)
                                                      cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACg                                                                                               >  1:546335/1‑150 (MQ=255)
                                                      cTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGAAAAGTCGTGGCTTACCGGGTTGGACTCAAGACCATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGCGGGTTCGTGCACACAGCCCAGCTTGGAGCGGAcc                                                                                               >  1:2313197/1‑149 (MQ=255)
                                                        cGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAc                                                                                             <  1:4342748/150‑1 (MQ=255)
                                                         gccctGCTAACCCTGTTACCAGGGGCTGCTGCCAGTGGCGGTAAGTCGTGTCTTACCGGGTTGGACCCAAGACGATAGTTACCGGATAAGGCAGAGCGGTCGGGCTGAACGGGGGGTCCGTGCACACAGCCCAGCTTGGAGCGAACGAcc                                                                                            <  2:3140057/147‑1 (MQ=255)
                                                         gCTCTGCTAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTAGGGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGAcc                                                                                            <  2:3689252/150‑1 (MQ=255)
                                                          ctctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCt                                                                                           >  1:3944457/1‑150 (MQ=255)
                                                           tctGCTAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTa                                                                                          <  2:2353843/150‑1 (MQ=255)
                                                            ctGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGGGCGAACGACCTac                                                                                         >  1:3461562/1‑150 (MQ=255)
                                                               cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAcc                                                                                      <  2:1725238/150‑1 (MQ=255)
                                                               cTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAcc                                                                                      <  2:2838764/150‑1 (MQ=255)
                                                                tAACCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCg                                                                                     <  2:1807081/150‑1 (MQ=255)
                                                                  aTCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCGAGACGATAGTTACCGGATGAGGCACAGCGGTCGGGCTGCACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGaa                                                                                   >  2:1628358/1‑150 (MQ=255)
                                                                   tCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAAc                                                                                  <  2:2535609/150‑1 (MQ=255)
                                                                    ccTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACt                                                                                 <  2:430055/150‑1 (MQ=255)
                                                                      tGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACCCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTga                                                                               <  1:3049605/150‑1 (MQ=255)
                                                                         tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAt                                                                            <  1:961768/150‑1 (MQ=255)
                                                                         tACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAt                                                                            >  1:2865582/1‑150 (MQ=255)
                                                                         tACCAGTGGATGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGAt                                                                            >  1:2064426/1‑150 (MQ=255)
                                                                          aCCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGGTa                                                                           >  1:2703325/1‑150 (MQ=255)
                                                                           ccAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAc                                                                          >  1:3208193/1‑150 (MQ=255)
                                                                            cAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATAcc                                                                         <  2:1284333/150‑1 (MQ=255)
                                                                               tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTAc                                                                      >  1:4027668/1‑150 (MQ=255)
                                                                               tGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTAc                                                                      <  2:1447074/150‑1 (MQ=255)
                                                                                ggCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCCCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACa                                                                     <  2:3036211/150‑1 (MQ=255)
                                                                                  ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGc                                                                   <  2:4738895/150‑1 (MQ=255)
                                                                                  ctgctgCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACCCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGc                                                                   <  1:1981805/150‑1 (MQ=255)
                                                                                   tgctgcCAGTGGCGATCAGTCGTGTCTTACCGGGTTGGACTCAAGACGCTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCg                                                                  <  1:4110901/150‑1 (MQ=255)
                                                                                    gctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGt                                                                 >  1:2639016/1‑150 (MQ=255)
                                                                                     ctgcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACCCAAGACGGTAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGAGTg                                                                <  2:2739586/150‑1 (MQ=255)
                                                                                       gcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATGGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAg                                                              <  1:4266394/150‑1 (MQ=255)
                                                                                       gcCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGGACGACCTACACCGAACTGGGGTACCTACAGCGTggg                                                              >  1:100/1‑148 (MQ=255)
                                                                                        ccAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGc                                                             >  2:820515/1‑150 (MQ=255)
                                                                                        ccAGTGGCGATAAGTCGTGACATACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACAGCGAACTGAGATACCTACAGCGTGAGc                                                             <  1:626782/150‑1 (MQ=255)
                                                                                          aGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTa                                                           >  1:712895/1‑150 (MQ=255)
                                                                                           gTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTAt                                                          <  2:913473/150‑1 (MQ=255)
                                                                                           gTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTAt                                                          <  2:2260678/150‑1 (MQ=255)
                                                                                            tGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATg                                                         >  1:3131944/1‑150 (MQ=255)
                                                                                               cGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATgaga                                                      >  2:3421775/1‑150 (MQ=255)
                                                                                                    aGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGCCACAGCGGTCGGGCCGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcg                                                 <  2:4658316/150‑1 (MQ=255)
                                                                                                     gTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGGACTGAGATACCTACAGCGTGAGCTATGAGAAGgcgc                                                >  1:796310/1‑150 (MQ=255)
                                                                                                     gTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAgcgc                                                <  2:3330132/150‑1 (MQ=255)
                                                                                                      tCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGcc                                               >  1:4601444/1‑150 (MQ=255)
                                                                                                         tgtCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACg                                            >  2:3508461/1‑150 (MQ=255)
                                                                                                             ttACCGGGTTGGACCCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATCCCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTc                                        <  2:627562/150‑1 (MQ=255)
                                                                                                              tACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGGGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTcc                                       >  1:1479469/1‑150 (MQ=255)
                                                                                                              tACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTcc                                       <  2:3871458/150‑1 (MQ=255)
                                                                                                              tACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGAGGCTTCGTCGACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTcc                                       >  1:4755751/1‑150 (MQ=255)
                                                                                                               aCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTccc                                      <  2:2634226/150‑1 (MQ=255)
                                                                                                               aCCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTccc                                      >  1:1564723/1‑150 (MQ=255)
                                                                                                                ccGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCg                                     <  1:3790875/150‑1 (MQ=255)
                                                                                                                 cGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGa                                    >  1:1658809/1‑150 (MQ=255)
                                                                                                                   ggTTGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAg                                  <  1:3297952/150‑1 (MQ=255)
                                                                                                                     ttGGACTCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAggg                                >  1:2551360/1‑150 (MQ=255)
                                                                                                                     ttGGACCCAAGACGATAGTTACCGGATAAGGCACAGCGGTCGGGCGGACCGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAACGCGCCACGCTTCCCGAAggg                                <  2:4469790/150‑1 (MQ=255)
                                                                                                                       ggACTCAAGACGATAGTTACCGGATAAGGCACAGCGGCCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGgag                              <  1:4433297/150‑1 (MQ=255)
                                                                                                                               gccGATAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTCCACACAGCCCAGGTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCCCGCTTCCCGAAGGGAGAAAGGCgg                      <  1:3868467/148‑1 (MQ=255)
                                                                                                                               gACGATAGTTCCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCgg                      <  2:1204785/150‑1 (MQ=255)
                                                                                                                                    tAGTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTCCACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAgg                 <  1:1899469/150‑1 (MQ=255)
                                                                                                                                      gTTACCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGGGAAAGGCGGACAGGTa               >  1:3619208/1‑150 (MQ=255)
                                                                                                                                         aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGATCTGAGATACCTACAGCGTGAGCTATGGGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGGCAGGTATcc            >  2:2067297/1‑150 (MQ=255)
                                                                                                                                         aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATcc            >  1:2947384/1‑150 (MQ=255)
                                                                                                                                         aCCGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATcc            >  1:1242732/1‑150 (MQ=255)
                                                                                                                                           cGGATAAGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCgg          <  1:4676795/150‑1 (MQ=255)
                                                                                                                                             gATAAGGCCCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTa        <  2:4136787/150‑1 (MQ=255)
                                                                                                                                                aaGGCACAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGc     <  2:1535550/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                   
GTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGC  >  pSIJ663_pHT315_araE_xy/5056‑5352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑