Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,336 T→C S50S (AGT→AGC)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,3360TC99.5% 1518.4 / NA 424S50S (AGT→AGC) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (262/159);  total (263/161)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 424 total aligned reads displayed.

GGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAA  >  CP006881/3723189‑3723481
                                                                                                                                                   |                                                                                                                                                 
   aaCTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATGTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTAttt                                                                                                                                              >  2:3945445/1‑150 (MQ=255)
       cAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTtcttt                                                                                                                                          >  2:1046921/1‑148 (MQ=255)
        aaGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTa                                                                                                                                         >  1:2547709/1‑150 (MQ=255)
         aGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTAt                                                                                                                                        <  2:4053126/150‑1 (MQ=255)
         aGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTAt                                                                                                                                        >  2:795568/1‑150 (MQ=255)
          gCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATg                                                                                                                                       <  1:2907881/150‑1 (MQ=255)
           cATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATgg                                                                                                                                      >  1:4389900/1‑150 (MQ=255)
            aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                                                                                                     <  2:1105666/150‑1 (MQ=255)
            aTTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGc                                                                                                                                     >  2:4678616/1‑150 (MQ=255)
             ttacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGct                                                                                                                                    >  2:4429739/4‑149 (MQ=255)
              tacAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGGAGCTATTTCTCTATGGctg                                                                                                                                   <  1:3080878/148‑3 (MQ=255)
               acAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGa                                                                                                                                  >  2:958454/2‑150 (MQ=255)
                 aaaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGAtt                                                                                                                                >  2:3600413/1‑150 (MQ=255)
                  aaagGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATtc                                                                                                                               <  2:2258623/150‑1 (MQ=255)
                     ggtcgAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTCATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATGTCTTAATGGCCGAATCTcc                                                                                                                            >  2:541608/5‑150 (MQ=255)
                     ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                            >  1:556894/2‑150 (MQ=255)
                     ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                            >  2:3191583/2‑150 (MQ=255)
                     ggTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTcc                                                                                                                            >  2:3685415/2‑150 (MQ=255)
                      gTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCA‑TTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGt                                                                                                                          <  1:2021533/150‑1 (MQ=255)
                       ttGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGCCTGATTCTCCGt                                                                                                                          <  1:2189741/150‑1 (MQ=255)
                           aCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGGTTCTCCGTTTGc                                                                                                                      >  2:2207545/1‑150 (MQ=255)
                           aCAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGc                                                                                                                      <  2:1760222/150‑1 (MQ=255)
                                  gCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCg                                                                                                               >  1:2816181/1‑150 (MQ=255)
                                    tttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGc                                                                                                             >  2:4209204/1‑150 (MQ=255)
                                     ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                            >  2:628588/1‑150 (MQ=255)
                                     ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                            >  2:1210958/1‑150 (MQ=255)
                                     ttGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGCCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCa                                                                                                            >  2:149492/1‑150 (MQ=255)
                                         aaaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCg                                                                                                        >  2:909915/3‑150 (MQ=255)
                                          aaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGGTTCTCCGATTGCCCTTCCGGCAGCCGc                                                                                                       >  2:2070316/2‑150 (MQ=255)
                                          aaaacaaaacaTATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGc                                                                                                       >  2:2743626/2‑150 (MQ=255)
                                            aaCAAAACATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTGGCCTTTCCGGGAGCCGCTg                                                                                                     >  2:675181/1‑150 (MQ=255)
                                             acaaaaCATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACTGCCGCAAGCAAAACAGGCTGCTATTTCTTTATGGCTGGTTCTCCGTTTGCCTTTCCGGCAGCCGCTGt                                                                                                    >  2:2623629/4‑150 (MQ=255)
                                             acaaaaCATATTCGCTCCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGt                                                                                                    <  1:2282790/147‑1 (MQ=255)
                                                   cATATTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACttt                                                                                              >  2:4850/1‑150 (MQ=255)
                                                     tatTCGCTTCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCg                                                                                            >  1:1871663/1‑150 (MQ=255)
                                                      attcgcttCAAGCAAAATCCGTTATGTGCTCCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTGCAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgg                                                                                           <  1:1351571/144‑1 (MQ=255)
                                                       ttcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCggg                                                                                          >  1:3347266/6‑150 (MQ=255)
                                                       ttcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCggg                                                                                          >  2:869382/6‑150 (MQ=255)
                                                        tcgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCgggg                                                                                         >  2:3532203/5‑150 (MQ=255)
                                                         cgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGc                                                                                        >  2:2825179/4‑150 (MQ=255)
                                                         cgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGc                                                                                        >  2:2689188/4‑150 (MQ=255)
                                                         cgcttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTGGCCTTTCCGGCAGCCGCTGTCCCTTTCggggg                                                                                        >  1:3439287/4‑149 (MQ=255)
                                                           cttCAAGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCg                                                                                      <  1:4111982/149‑1 (MQ=255)
                                                              caaGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTa                                                                                   <  2:3461783/148‑1 (MQ=255)
                                                                aGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                 >  2:3345694/1‑150 (MQ=255)
                                                                aGCAAAATCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                 <  1:1432131/150‑1 (MQ=255)
                                                                aGCAAAATCCGTTATGTGCTTCCGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTAtt                                                                                 >  1:353748/1‑150 (MQ=255)
                                                                    aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctga                                                                             <  1:3198547/150‑2 (MQ=255)
                                                                    aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCCATTctgg                                                                             >  1:4315011/1‑149 (MQ=255)
                                                                    aaaTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCCGTCACTTTCGGGGCCGCGATTctga                                                                             >  1:1250235/1‑149 (MQ=255)
                                                                      aTCCGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatg                                                                           >  2:2975731/1‑150 (MQ=255)
                                                                      aTCCGTTATGTGCTTCGGCCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatg                                                                           <  1:4469085/150‑1 (MQ=255)
                                                                        ccGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTctgatggt                                                                         >  1:3956448/1‑148 (MQ=255)
                                                                         cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTCCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                        <  2:3633962/150‑1 (MQ=255)
                                                                         cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                        <  1:1008748/150‑1 (MQ=255)
                                                                         cGTTATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGAtt                                                                        >  1:2486908/1‑150 (MQ=255)
                                                                           ttATGTGCTTCGGTCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGc                                                                      <  2:1871860/150‑1 (MQ=255)
                                                                                   ttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAg                                                              <  2:4115819/145‑1 (MQ=255)
                                                                                   ttcggtCCATTTTGGCCAGTATATTTATTGGTTTTGCCATTCCAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAg                                                              <  1:2691440/145‑1 (MQ=255)
                                                                                    tcggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAgg                                                             <  2:2119312/146‑1 (MQ=255)
                                                                                    tcggtCCATTTTGGCCAGTATATTTATTGG‑TTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGc                                                            >  2:1987734/5‑150 (MQ=255)
                                                                                      ggtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGgggggg                                                           >  2:1582892/3‑148 (MQ=255)
                                                                                       gtCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCgg                                                          <  1:4022205/149‑1 (MQ=255)
                                                                                        tCCATTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCggg                                                         >  1:3690941/1‑149 (MQ=255)
                                                                                         ccATTTTGGCCAGTATATTTATTGGTTTTGACATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGGAGTTCCGCTGGTTGCTTACTGAGGTgggg                                                        >  2:1612309/1‑148 (MQ=255)
                                                                                           aTTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCATGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGgaggt                                                      >  1:3494201/1‑148 (MQ=255)
                                                                                           aTTTTGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGat                                                      >  1:868332/1‑150 (MQ=255)
                                                                                             tttGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCCGGGCCGCTATGCTGATGAGTGCTTACGGAGGCGGCGattt                                                    >  2:4351079/1‑150 (MQ=255)
                                                                                               tGGCCAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGAGGCGGAGatttat                                                  >  1:4703625/1‑150 (MQ=255)
                                                                                                  ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGGTTGCTTACGGAGGCGGGGatttatttt                                               >  2:28574/1‑149 (MQ=255)
                                                                                                  ccAGTATATTTATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGAtttattta                                               <  2:1258752/150‑1 (MQ=255)
                                                                                                     gtatatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTAccg                                            <  1:1546816/145‑3 (MQ=255)
                                                                                                       atatttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGGGGTTTATTTACCGGc                                          >  1:3258046/4‑150 (MQ=255)
                                                                                                        tatctATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTGTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCa                                         <  2:4567850/146‑1 (MQ=255)
                                                                                                         atttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGGGGCGGAGATTTATTTACCGGCaa                                        >  2:450566/2‑150 (MQ=255)
                                                                                                         atttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCaa                                        >  2:2376667/2‑150 (MQ=255)
                                                                                                         atttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGCGCCGCCATTCTGTTGATTGCTTACGGAGGCGCAGATTTATTTACCGGCaa                                        >  1:1656677/2‑150 (MQ=255)
                                                                                                          tttATTGGTTTTGGCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTTCCGGCAac                                       >  1:748937/1‑150 (MQ=255)
                                                                                                          tttATTGGTTTTGGCATTACAGCCCCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAac                                       <  1:947649/150‑1 (MQ=255)
                                                                                                           ttATTGGGTTTGGCATTACAGCCGCAGGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGAGGCGGGGAGTTATTTACCGGCAaca                                      >  2:1913003/1‑150 (MQ=255)
                                                                                                            tATTGGTTTTGGCCTTCCAGCCCCAAGCAAAACAGGCAGGTGTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAacac                                     <  2:2565544/150‑1 (MQ=255)
                                                                                                            tATGGGTTTCGGCATTACAGCCGCAAGCAAACCAGGCACCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAacac                                     <  1:3819943/150‑1 (MQ=255)
                                                                                                                ggttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGACACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACcttt                                 <  1:2398960/144‑1 (MQ=255)
                                                                                                                 gttttggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGGCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACctttt                                >  2:2510865/6‑150 (MQ=255)
                                                                                                                      ggCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTc                           <  1:3391202/150‑1 (MQ=255)
                                                                                                                      ggCATAACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTc                           <  1:2099632/150‑1 (MQ=255)
                                                                                                                      ggCACTACAGCCGCAAGCAAACCAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTc                           <  2:2308982/150‑1 (MQ=255)
                                                                                                                       gCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCa                          >  2:3256298/1‑150 (MQ=255)
                                                                                                                       gCATTACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCa                          >  2:1655354/1‑150 (MQ=255)
                                                                                                                          ttACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGGTGATTGCTTACGGTGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCAcct                       >  1:2780624/1‑148 (MQ=255)
                                                                                                                           tACAGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCCCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCta                      <  1:4218176/150‑1 (MQ=255)
                                                                                                                             cctccGCAGACAAACCAGGCAGCTATTTCTTTATGGCAGACTCTCCGCTTGCCTTTCCGTCAGCCGCTGTCACTTTCGGGGCCGTTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACAACTTTTATTTCATCtata                    <  1:257233/147‑1 (MQ=255)
                                                                                                                              aGCCGCAAGCAAAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAc                   >  2:3057661/1‑150 (MQ=255)
                                                                                                                              aGCCCCAAGCAAACCAGGCAGCTTTTCCATTATGGCGGATGCCCCGTTTGCCTTTCCGGCAGCCGCAGCCACTTCCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATAc                   <  2:3780508/150‑1 (MQ=255)
                                                                                                                                     agcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGGTTGCTTACGGAGGCGGAGATTTATTTTCCGGCAACACCCTTTAATTCACCTAAACCCCGCTc            >  2:3886517/1‑150 (MQ=255)
                                                                                                                                      gcaaAACAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCCATTCTGATGGTTGCTTACGGAGGCGGGGATTTATTTACCGGCAACACCTTTTATTTCACCTTTACcgcccccc           >  1:98238/1‑145 (MQ=255)
                                                                                                                                            cAGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaa     <  1:1804976/150‑1 (MQ=255)
                                                                                                                                             aGGCAGCTATTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTGATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaaa    >  2:4274823/1‑150 (MQ=255)
                                                                                                                                              ggCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGCGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATATAAATACCGGCTACACCTTTTATTTCACCTATACCGCGCACCGgaaaaaa   <  2:1318686/150‑1 (MQ=255)
                                                                                                                                               gCAGCTTTTTCTTTATGGCTGATTCTCCGTTTGCCTTTCCGGCAGCCGCTGTCACTTTCGGGGCCGCTATTCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGgaaaaaaa  <  1:3302071/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGTTATGTGCTCAGATCCATTTTGGCCAGCATGTTTATCGGATTCGGCATTACAGCCGCAAGCAAAACAGGCAGTTATTTCTCTATGGCGGATTCTCCGTTTGCTTTTCCGGCAGCCGCTGTCACATTCGGGGCCGCTATTCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAA  >  CP006881/3723189‑3723481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑