Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006881 3,723,549 A→G L121L (CTA→CTG)  U712_19195 → putative transporter ywcJ

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,723,5490AG98.6% 1340.0 / ‑5.8 361L121L (CTA→CTG) U712_19195putative transporter ywcJ
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (145/210);  minor base T (0/5);  total (146/215)
Fisher's exact test for biased strand distribution p-value = 8.47e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 361 total aligned reads displayed.

TCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGC  >  CP006881/3723402‑3723695
                                                                                                                                                   |                                                                                                                                                  
tCTGTTGATTGCTTACGGGGGCGGAGATTTATTTCCCGGCACCCCCTTTCTTTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtt                                                                                                                                                  <  2:1656677/150‑1 (MQ=255)
tCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtt                                                                                                                                                  <  2:2816181/150‑1 (MQ=255)
tCTGATGATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGtt                                                                                                                                                  <  1:1604280/150‑1 (MQ=255)
   gatgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTATTCGGCGCCATTCTGTTTGc                                                                                                                                               >  2:4317201/3‑150 (MQ=255)
   gatgATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGc                                                                                                                                               <  1:4850/148‑1 (MQ=255)
    atgATTGCCTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGcc                                                                                                                                              <  1:4428967/149‑1 (MQ=255)
     tgATTGCATACGGAGGCGGAGATTTATTTACCGGCCACACCTTTTATTTCACCTTTACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTGTACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCa                                                                                                                                             <  1:2838217/150‑1 (MQ=255)
      gATTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAt                                                                                                                                            >  1:34153/1‑150 (MQ=255)
       aTTGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAtc                                                                                                                                           <  2:4159166/150‑2 (MQ=255)
        ttGCTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCAtcc                                                                                                                                          <  1:3532203/150‑3 (MQ=255)
           cTTACGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGa                                                                                                                                       <  1:2936775/150‑1 (MQ=255)
               cGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAg                                                                                                                                   >  2:2189086/1‑150 (MQ=255)
               cGGAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAg                                                                                                                                   >  2:1431556/1‑150 (MQ=255)
                ggAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgc                                                                                                                                  >  2:3319371/1‑150 (MQ=255)
                 gAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATTCCGCGCTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGGGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcg                                                                                                                                 >  2:4083708/1‑150 (MQ=255)
                 gAGGCGGAGATTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAgcg                                                                                                                                 <  1:2740164/150‑1 (MQ=255)
                        agaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                          <  2:737434/150‑1 (MQ=255)
                        agaTTTATTTACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATCTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACggg                                                                                                                          >  2:3910713/1‑150 (MQ=255)
                          atttatttACCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACgggac                                                                                                                        <  1:1059350/150‑3 (MQ=255)
                           tttatttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACt                                                                                                                       <  1:2240387/150‑1 (MQ=255)
                              atttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACtttt                                                                                                                    >  2:866863/1‑150 (MQ=255)
                                ttaCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTg                                                                                                                  <  1:3086436/150‑1 (MQ=255)
                                 taCGGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTga                                                                                                                 <  1:4529619/150‑1 (MQ=255)
                                 taCCGGCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTga                                                                                                                 <  1:3814171/150‑1 (MQ=255)
                                  accggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgag                                                                                                                >  1:1686186/4‑150 (MQ=255)
                                     ggCACCACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgaggag                                                                                                             <  2:3706298/150‑1 (MQ=255)
                                     ggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgaggag                                                                                                             >  1:166181/1‑150 (MQ=255)
                                     ggCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTgaggag                                                                                                             <  1:3346574/150‑1 (MQ=255)
                                      gCAACACCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGGCTTTTTgaggagc                                                                                                            >  2:2144109/1‑149 (MQ=255)
                                        aaCACCTTTTATTTCACCTATGCCGCGCTCGGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCt                                                                                                          <  1:1157054/150‑1 (MQ=255)
                                         acacCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCtt                                                                                                         >  1:1433950/1‑150 (MQ=255)
                                          cacCTTTTATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAAATTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCttt                                                                                                        >  1:1808514/1‑149 (MQ=255)
                                           accttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCt                                                                                                       <  2:3489489/147‑1 (MQ=255)
                                            ccttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTg                                                                                                      <  2:353748/148‑1 (MQ=255)
                                             cttttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGGGGGGCCTTCTgg                                                                                                     >  2:1691886/2‑149 (MQ=255)
                                              ttttATTTCACCTATACCGCGCTCCGGAAAAATATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGtt                                                                                                    <  1:1012344/150‑1 (MQ=255)
                                               tttATTTCCCCTATACCGGGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAACCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTc                                                                                                   <  1:86459/150‑1 (MQ=255)
                                                ttATTTCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCa                                                                                                  >  2:4700259/1‑150 (MQ=255)
                                                    ttCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTcc                                                                                              <  1:4074844/150‑1 (MQ=255)
                                                     tCACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCt                                                                                             >  1:1338090/1‑150 (MQ=255)
                                                      cACCTATACCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtt                                                                                            <  1:2130330/150‑1 (MQ=255)
                                                       acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGGCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTATTCGGCGCCATTCTGTTTGCCATCCTGGTCAGCGCGACGGGCCTTTTTGTGGAGCCTTCTGTTCATTCCttt                                                                                           >  1:652665/4‑150 (MQ=255)
                                                       acctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTCCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCttt                                                                                           >  2:4422679/4‑150 (MQ=255)
                                                        cctataCCGGGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCtttt                                                                                          <  1:2689188/148‑1 (MQ=255)
                                                        cctataCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACCCCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTATGAGGAGCCTTCTGTTCATTCCtttt                                                                                          <  2:3956448/148‑1 (MQ=255)
                                                             aCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGAtt                                                                                     <  2:1238354/150‑1 (MQ=255)
                                                             aCCGCGCTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTGTGAtt                                                                                     >  2:3666709/1‑150 (MQ=255)
                                                               cgcgCTCCGGGAAAAAATCAGCTGGGGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAAATTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTATTTGAGGTGCCTTCTGTTCATTCCTTTTTGTTTCa                                                                                   >  1:1478071/1‑150 (MQ=255)
                                                               cccccTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCa                                                                                   <  2:3354552/146‑1 (MQ=255)
                                                                 cgcTCCGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGGTTGCCATCCTGATCAGCGCGACGGGACTTTTTGGGGAGCCTTCTGTTCATTCCTTTTTGATTCAtt                                                                                 >  2:263746/1‑149 (MQ=255)
                                                                   ccccGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTg                                                                               <  2:1936350/148‑1 (MQ=255)
                                                                   cTCCGGAAAAAAATCAGCTGGCGCGCCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTTCTTTTTGATTCATTTg                                                                               <  1:4718906/150‑1 (MQ=255)
                                                                     ccGGAAAAAAATCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGc                                                                             <  2:2958965/150‑1 (MQ=255)
                                                                      cggaaaaaaaTCAGCTGGCGCGACACCCTATTCTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                                            <  1:2586115/147‑1 (MQ=255)
                                                                      cggaaaaaaaCCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCa                                                                            <  2:3258046/147‑1 (MQ=255)
                                                                           aaaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGca                                                                       <  1:2975731/150‑1 (MQ=255)
                                                                            aaaaTCAGCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGcac                                                                      <  2:605332/150‑1 (MQ=255)
                                                                             acatcagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGcaca                                                                     <  1:4139043/144‑1 (MQ=255)
                                                                             aaatcagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGcaca                                                                     <  1:4677172/144‑1 (MQ=255)
                                                                               atcagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTCGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGGACaaa                                                                   >  1:3548743/5‑150 (MQ=255)
                                                                                ccagCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATGTAATGGGCGCCATTCAGTTTGCCATCCTGATCGGCGCGACGGGACTTTTAGAGGAGCCTTCCGTTCATTCCTTTTTGATTCATTTGGCAGAGCACaaaa                                                                  <  1:3246003/147‑1 (MQ=255)
                                                                                  agCTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGTCTTCTGTTCATTCCTTTTTGATTCAT‑TTGCAGAGCACAAAATgg                                                               <  2:1345240/149‑1 (MQ=255)
                                                                                    cTGGCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATGTGGCAGAGCCCAAAATGGa                                                              <  1:185530/150‑1 (MQ=255)
                                                                                      ggCGCGCCACCCTATCCTTGGGGATGTCATGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgc                                                            <  1:1641715/150‑1 (MQ=255)
                                                                                      ggCGCGACACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgc                                                            >  2:1515917/1‑150 (MQ=255)
                                                                                      gccgcgaCACCCTATACTTGTGGATGACAAGCTAGGCCGGCAATTTAAGCGGCGCCTTTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTATTGAGGAGCCTTCTGTTCCTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgc                                                            <  1:3908197/145‑1 (MQ=255)
                                                                                         gcgaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAgccgc                                                         >  2:822503/3‑150 (MQ=255)
                                                                                           gaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGGGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGGgccgccg                                                       >  2:2739546/1‑149 (MQ=255)
                                                                                           gaCACCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCACTCCTTTTTGATTCATTTGGCAGTGCACAAAATGGGgccgccg                                                       >  1:2500303/1‑149 (MQ=255)
                                                                                             cacCCTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGccgccggc                                                     >  1:72302/1‑150 (MQ=255)
                                                                                                ccTATACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTc                                                  >  2:1074943/1‑150 (MQ=255)
                                                                                                ccTATACTTGTGGATGCCAAGCTAGGCCGGCAATTTAACCGGCGCCATTCTGTGTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTc                                                  <  1:2198091/150‑1 (MQ=255)
                                                                                                 ctatACTTGTGTATGTAAAGCTTTCCAGGCAATTTAACCGGCGCCTTTCTGTGTGCCACCCTGAACAGCGCGACGGGATTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATCCATTTGCCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                 <  2:1865437/147‑1 (MQ=255)
                                                                                                 ctatACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTcc                                                 >  2:3508416/4‑150 (MQ=255)
                                                                                                   ttACTTGTGGATACCAAGCTATGCCGGCAATTTAATCGGCGCCATCCTGTTTGCCATCCTGATCGGGGCGACGGGACTTTTTGAGGACCCTTCTGTTCATCCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGa                                               <  1:3595064/149‑1 (MQ=255)
                                                                                                    tACTTGTGGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaa                                              >  2:2421041/1‑150 (MQ=255)
                                                                                                     acttgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGaat                                             >  2:4225233/4‑149 (MQ=255)
                                                                                                        tgtgGATGTCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGt                                          >  1:1223753/1‑150 (MQ=255)
                                                                                                          gggATGCCAAGCTATGCCGGCAATTTATTCGGCGCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATCCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGttt                                        <  1:1531671/149‑1 (MQ=255)
                                                                                                                tCAAGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGACCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCaga                                  <  1:2041687/150‑1 (MQ=255)
                                                                                                                   aGCTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGGGCACAAAATGGAGCCGCCGGCTTCCGGATTGTTTTTCAgggga                               >  2:484102/1‑149 (MQ=255)
                                                                                                                   aGCTATGCCGGCAATTTAATCGCCGCCATTCTGTTTGCCATCCTGATCAGCGGGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCTTTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAgga                               <  1:111389/150‑2 (MQ=255)
                                                                                                                     cTATGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGGGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAAt                             >  2:2258278/1‑150 (MQ=255)
                                                                                                                      tATGCCGGCATTTTAATCGGCGCCATTCTGTTTGCCTTCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTTTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATg                            <  1:2289165/150‑1 (MQ=255)
                                                                                                                        tGCCGGCATTTTACTCGGCGCCATTCTGTTTGCCATCCGGCCCAGCGCGCCGGGACTTTTTGGGGAGCCTTCTGTTCTTTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCt                          <  1:4622966/150‑1 (MQ=255)
                                                                                                                        tGCCGGCAATTTAATGGGCGCCATCCTGTTTGCCACCCAGATCAGCGCGACGGGACTTGTGGAGGAGCCTTCGGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCt                          <  1:4317201/150‑1 (MQ=255)
                                                                                                                        tGCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACATAATGGAGCCGCCGGCTTCCGAATTGGTTTTCAGAGGAATGCt                          >  1:1266184/1‑150 (MQ=255)
                                                                                                                         gCCGGCAATTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCACAGGAATGCtg                         >  2:400272/1‑150 (MQ=255)
                                                                                                                           cGGCAATTTAATCGGCGCCATTCTGTCTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCtgtg                       <  1:1978304/150‑1 (MQ=255)
                                                                                                                             gCAATTTAATCGGCCCCATTCTGTTTGCCATCCTGATCGGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCa                     <  1:3034285/150‑1 (MQ=255)
                                                                                                                               aaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGGATTGATTTTCAGAGGAATGCTGTGCAAt                   >  2:3142255/1‑150 (MQ=255)
                                                                                                                               aaTTTAATCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAAt                   >  2:3082467/1‑150 (MQ=255)
                                                                                                                                  ttcaTCGGCGCCATTCTGTTTGCCATCCTGAGCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTgg                <  2:3799362/147‑1 (MQ=255)
                                                                                                                                   tactCGGCGCCATTCTGTTTCCCATCCTGATCAGCGCGACGGGACTTTTTGAGGACCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGc               <  2:1733512/147‑1 (MQ=255)
                                                                                                                                   taaTCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACACAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGGGCAATTGGc               >  1:949617/3‑150 (MQ=255)
                                                                                                                                     aTCGGCGCCATTCTCTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCtt             >  1:422489/1‑150 (MQ=255)
                                                                                                                                     aTCGGCCCCATTCTGTTTGCCATCTTAGTCAGGGCGCCGGGACTTTTTGAGGCCCCTTCTGTCCATCCCTTTTTGATTCTTTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCtt             <  2:4550015/150‑1 (MQ=255)
                                                                                                                                      tCGGCGCCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGGGCAATAGGCTtg            >  1:447834/1‑150 (MQ=255)
                                                                                                                                         gcgcCATTCTGTTTGCCATCCTGATCAGCGCGACGGGACTTTTTGAGGAGCCTTCTGTTCATTCCTTTTTGATTCATTTGGCAGAGCACAAAATGGTGCCGCCGGCTTCCCAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTtgtgt         >  1:428769/1‑150 (MQ=255)
                                                                                                                                              aTTCTTTTTGCCCTCCTGATCAGCGGGACGGGACTTTGTGGGGACCCTTCTGTCCATTCCTTTTTGACCCATTTGGCAGAGCACAAAATGGAGCCGCCGGCTTCCGAATTGTTTTTCAGAGGAATGCTGTGCAATTGGCTTGTGTGCCTc    <  1:2400498/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCTGCTGATTGCTTACGGAGGCGGAGATTTATTTACTGGCAACACTTTTTATTTCACTTATACCGCGCTCCGAAAAAAAATTCGCTGGCGAGACACCCTGTACCTGTGGATGTCAAGCTATGCCGGCAATTTGATCGGCGCCATTCTATTTGCCATTCTGATCAGCGCGACAGGGCTTTTTGAGGAGGCTTCTGTTCATTCCTTTTTGATTCATCTGGCAGAGCACAAAATGGAGCCGCCCGCTTCCGAACTGTTTTTCAGAGGGATGCTGTGCAATTGGCTTGTGTGCCTCGC  >  CP006881/3723402‑3723695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑