Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,725,205:1 +T 100% intergenic (‑37/‑492) U712_19205 ← / → U712_19210 Uncharacterized protein ywcI/Minor extracellular protease vpr

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,725,2051.T97.5% 1495.9 / 28.4 436intergenic (‑37/‑492)U712_19205/U712_19210Uncharacterized protein ywcI/Minor extracellular protease vpr
Reads supporting (aligned to +/- strand):  ref base . (5/6);  new base T (226/199);  total (231/205)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Polymorphic indel expands or contracts a homopolymer stretch.

Only 100 of 459 total aligned reads displayed.

TGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCC‑TTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAC  >  CP006881/3725056‑3725349
                                                                                                                                                       |                                                                                                                                                
 ggTATGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTCTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCC‑t                                                                                                                                                 <  1:2628610/150‑1 (MQ=255)
    aTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCC‑tttt                                                                                                                                              <  1:3107912/150‑1 (MQ=255)
    aTGTACGCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCC‑tttt                                                                                                                                              >  2:3467675/1‑150 (MQ=255)
          gCTGAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTagcag                                                                                                                                         <  2:3953255/150‑1 (MQ=255)
             gAGCCGAATAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGGAGccc                                                                                                                                      >  1:4792421/1‑150 (MQ=255)
                  gAATAGACCGCGGCAGGCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTTGCAGCCCGtttt                                                                                                                                 >  1:5326110/1‑150 (MQ=255)
                    aTAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTtct                                                                                                                               >  2:5138734/1‑150 (MQ=255)
                     tAGACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTtctc                                                                                                                              <  2:4853280/150‑1 (MQ=255)
                       gACCGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAg                                                                                                                            <  2:3292374/150‑1 (MQ=255)
                        accgCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGc                                                                                                                           >  2:1188937/4‑150 (MQ=255)
                          cgCGGCAGCCGCAGTTTCTCTGTCCGGCGACACCCAGTTCACCAAAAAGACCATCCACACCCGTAAAGATACAAGCACACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑TCCTTTTTAGCAGCCCGTTTTCTCAGCCg                                                                                                                         <  2:461514/149‑1 (MQ=255)
                             ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGcgg                                                                                                                      >  2:1556255/1‑148 (MQ=255)
                             ggCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAg                                                                                                                      <  2:4958120/150‑1 (MQ=255)
                                  ccGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAGACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCTGGCG‑GCCTTTTTAGCAGCCCGTTTTCTCTGCCGGTGcccgc                                                                                                                 >  1:648654/1‑148 (MQ=255)
                                   cGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGcccgca                                                                                                                >  1:5131574/1‑147 (MQ=255)
                                       gTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGcccgcaagaa                                                                                                            >  1:341363/1‑143 (MQ=255)
                                       gTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGGAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTGTTCTCAGCCGCAGcccgcaagaa                                                                                                            >  2:4318723/1‑143 (MQ=255)
                                              tgtgCGGCGTCCCCCAGTTCATCAAAAAGACCATCCACAGCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCAGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACgg                                                                                                     <  1:1454664/150‑1 (MQ=255)
                                               gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc                                                                                                    >  2:5726078/1‑150 (MQ=255)
                                               gTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGc                                                                                                    <  1:259052/150‑1 (MQ=255)
                                                tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGcc                                                                                                   >  2:2927845/1‑150 (MQ=255)
                                                tCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGcc                                                                                                   <  1:3624261/150‑1 (MQ=255)
                                                  cGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGa                                                                                                 <  2:5269853/150‑1 (MQ=255)
                                                     cGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAttt                                                                                              <  2:385416/150‑1 (MQ=255)
                                                        cACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAATACGGCCGAtttctt                                                                                           <  2:1817566/150‑1 (MQ=255)
                                                         aCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt                                                                                          <  1:4634265/150‑1 (MQ=255)
                                                         aCCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttt                                                                                          >  1:5361311/1‑150 (MQ=255)
                                                           ccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttc                                                                                        >  1:50662/1‑150 (MQ=255)
                                                           ccAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttc                                                                                        <  1:4526063/150‑1 (MQ=255)
                                                            cAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAATATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttct                                                                                       >  1:5837834/1‑150 (MQ=255)
                                                            cAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGAtttcttttct                                                                                       <  1:4296865/150‑1 (MQ=255)
                                                            cAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCCAtttcttttct                                                                                       >  1:3742010/1‑150 (MQ=255)
                                                             aGTACACCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTCCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTtctc                                                                                      <  2:5444472/150‑1 (MQ=255)
                                                              gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                                                     >  1:866732/1‑150 (MQ=255)
                                                              gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                                                     <  2:1625456/150‑1 (MQ=255)
                                                              gTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCg                                                                                     <  2:2411487/150‑1 (MQ=255)
                                                               ttCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGc                                                                                    >  1:3169537/1‑150 (MQ=255)
                                                                 catcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGACCGGTTTCTTTTCTCGCaa                                                                                  >  1:1250386/1‑150 (MQ=255)
                                                                  atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCaaa                                                                                 <  1:2971427/150‑1 (MQ=255)
                                                                  atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCaaa                                                                                 <  2:4078520/150‑1 (MQ=255)
                                                                  atcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCaaa                                                                                 <  2:3150067/150‑1 (MQ=255)
                                                                   tcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc                                                                                >  2:5583284/1‑150 (MQ=255)
                                                                   tcaAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAAc                                                                                <  2:5047288/150‑1 (MQ=255)
                                                                      aaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAg                                                                             >  1:4708094/1‑150 (MQ=255)
                                                                      aaaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAg                                                                             >  2:3623886/1‑150 (MQ=255)
                                                                       aaaGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGc                                                                            >  2:4396947/1‑150 (MQ=255)
                                                                            ccATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCTCAACCTAGCGGctc                                                                       >  1:2783807/1‑150 (MQ=255)
                                                                            ccATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGctc                                                                       <  2:377639/150‑1 (MQ=255)
                                                                             cATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAAACGGCCGAGTTCTTTTCTCGCAAAGTAGCGGgtct                                                                      >  1:5694304/1‑150 (MQ=255)
                                                                              aTCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTa                                                                     >  2:2237906/1‑150 (MQ=255)
                                                                               tCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAg                                                                    <  2:4128587/150‑1 (MQ=255)
                                                                                    acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                               >  2:5682033/1‑150 (MQ=255)
                                                                                    acCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCaaaa                                                               <  2:4737004/150‑1 (MQ=255)
                                                                                     cccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTACCGGCTCTAGCAAAAc                                                              >  1:734823/1‑150 (MQ=255)
                                                                                      ccGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCCGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGACGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACt                                                             >  2:2885482/1‑150 (MQ=255)
                                                                                       cGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGGAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTc                                                            >  1:1058647/1‑150 (MQ=255)
                                                                                        gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                                           <  1:1373951/150‑1 (MQ=255)
                                                                                        gTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCa                                                           >  2:2692957/1‑150 (MQ=255)
                                                                                           aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                                        >  2:3323840/1‑150 (MQ=255)
                                                                                           aaGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGa                                                        >  1:3277331/1‑150 (MQ=255)
                                                                                                 cAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTAt                                                  >  1:3041665/1‑150 (MQ=255)
                                                                                                 cAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTAt                                                  <  1:2419457/150‑1 (MQ=255)
                                                                                                 cAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTAt                                                  <  2:4221377/150‑1 (MQ=255)
                                                                                                 cAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTAt                                                  >  2:621570/1‑150 (MQ=255)
                                                                                                  aaGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATg                                                 >  2:2829579/1‑150 (MQ=255)
                                                                                                   aGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGa                                                >  2:2061476/1‑150 (MQ=255)
                                                                                                     cAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATa                                              <  1:5228834/150‑1 (MQ=255)
                                                                                                     cAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATa                                              >  1:1955986/1‑150 (MQ=255)
                                                                                                      aaaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAg                                             <  1:3929549/150‑1 (MQ=255)
                                                                                                       aaCGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGa                                            >  1:4128661/1‑150 (MQ=255)
                                                                                                         cGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGAcc                                          >  1:2894122/1‑150 (MQ=255)
                                                                                                          gTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCg                                         >  1:1915931/1‑150 (MQ=255)
                                                                                                              cATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGAAAAACTCATGACTCTATAAAAAACCATGGa                                     >  1:2282350/1‑150 (MQ=255)
                                                                                                               aTAGAAAAACATCCCTCCGCTTCTTTTTGGCAGTCA‑GCCTTTTTAGCAGCCCGTCTTCTCAGCCGCAGCCCGCAAGACAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCGAAACTCATGACTCTATGATAGACCGTGGaa                                    <  2:426068/150‑1 (MQ=255)
                                                                                                                 aGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAag                                  >  2:4701018/1‑150 (MQ=255)
                                                                                                                 aGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAag                                  >  1:1758034/1‑150 (MQ=255)
                                                                                                                  gAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAaga                                 >  2:1704275/1‑150 (MQ=255)
                                                                                                                    aaaaCATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAgaga                               <  2:1363087/150‑1 (MQ=255)
                                                                                                                        cATCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTc                           <  2:1462786/150‑1 (MQ=255)
                                                                                                                         atgccTCCGCTTCTTTTTGGCAGGCAGGCT‑TTTTAGGAGCCCGTTTTCTCAGCCGCACCCCGCAAGAAAAGACGACCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAAATCt                          >  1:4659067/4‑150 (MQ=255)
                                                                                                                         aTCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                          >  2:3404051/1‑150 (MQ=255)
                                                                                                                         aTCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCt                          >  2:70162/1‑150 (MQ=255)
                                                                                                                         aTCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAAACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGGCTCTATGATAGACCGTGGAAAGAGAATTCt                          >  1:1213909/1‑150 (MQ=255)
                                                                                                                          tCCCTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCtt                         >  1:5563507/1‑150 (MQ=255)
                                                                                                                           cccTCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGCTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCttt                        <  2:5310509/150‑1 (MQ=255)
                                                                                                                              tCCGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTc                     >  1:182465/1‑150 (MQ=255)
                                                                                                                                cGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcac                   <  2:1313774/150‑1 (MQ=255)
                                                                                                                                cGCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcac                   <  1:3364271/150‑1 (MQ=255)
                                                                                                                                 gCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcaca                  >  2:2520393/1‑150 (MQ=255)
                                                                                                                                 gCTTCTTTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCCCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTcaca                  <  1:3506759/150‑1 (MQ=255)
                                                                                                                                  cttcttTTTGGCAGGCA‑TCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGGAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACCCATGTCTCTATGATAGACCGTGGTAAGAGAATTTTTTGTCNCaa                 <  2:1895736/150‑1 (MQ=255)
                                                                                                                                   ttcttTTTGGCGGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAAATCATGACTCTATGATAGACCGTGGAAAGAGAATTGTTTGTCACAat                >  1:1527033/1‑150 (MQ=255)
                                                                                                                                   ttcttTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAat                <  2:1058647/150‑1 (MQ=255)
                                                                                                                                     cttTTTGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAatat              >  1:5644008/1‑150 (MQ=255)
                                                                                                                                       ttttGGCAGGCA‑GCCTTTTTAGCAGCCCGTTTGCTCAGCCGGAGCCCCCAAGAAAAGACGGCCGATTCCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGa            <  1:2863312/150‑1 (MQ=255)
                                                                                                                                            gcaggca‑gCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGGCGATTTCTTTTTTCGCAAACTAGCGGCTCTAGAAAAACTCATAACTCTATGATAGACCGTGGAAAAAGTATTTTTTGTCACAATATGAGTGaa       >  1:1665603/1‑150 (MQ=255)
                                                                                                                                            gcaggca‑gCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaa       <  2:774451/150‑1 (MQ=255)
                                                                                                                                             caggca‑gCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaa      <  1:4321015/150‑1 (MQ=255)
                                                                                                                                              aggca‑gCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGaaaa     <  1:5726078/150‑1 (MQ=255)
                                                                                                                                                 ca‑gCCTTTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCGCAAGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAc  <  1:2216836/150‑1 (MQ=255)
                                                                                                                                                       |                                                                                                                                                
TGGTATGTACGCTGAGCCGAATAGACAGCGGCAGCCGCAGTTTTTCTGTCCGGCGTCACCCAGTTCATCAAAAAGACCATCCACACCCGTAAAGATACAAGCAAACGTTTCATAGAAAAACATCCCTCCGCTTCTTTTTGGCAGGCA‑GCC‑TTTTAGCAGCCCGTTTTCTCAGCCGCAGCCCACGGGAAAAGACGGCCGATTTCTTTTCTCGCAAACTAGCGGCTCTAGCAAAACTCATGACTCTATGATAGACCGTGGAAAGAGAATTCTTTGTCACAATATGAGTGAAAAAAC  >  CP006881/3725056‑3725349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑