| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | pSIJ663_pHT315_araE_xy | 5,478 | G→A | 100% | intergenic (+747/–) | ampR → / – | –/– |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | pSIJ663_pHT315_araE_xy | 5,478 | 0 | G | A | 100.0% | 43635.9 / ‑2.5 | 12801 | intergenic (+747/–) | ampR/– | –/– |
| Reads supporting (aligned to +/- strand): ref base G (51/5); new base A (6286/6425); total (6363/6438) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-11 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
Only 100 of 12803 total aligned reads displayed.
CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATGACACAAAA > pSIJ663_pHT315_araE_xy/5330‑5624 | ggACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAg < 2:2640564/150‑1 (MQ=255) ggACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTAATGCTCGTCAGGGGGGCGAAg < 1:40774/150‑1 (MQ=255) aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGcc > 1:530574/1‑150 (MQ=255) aCAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGcc > 1:2287769/1‑150 (MQ=255) cAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCt < 2:1987922/150‑1 (MQ=255) tATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATgg > 1:3788382/1‑150 (MQ=255) tATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATgg < 2:5239859/150‑1 (MQ=255) ggTAAGCGGCAGGGTGGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGaaaaa < 2:209449/150‑1 (MQ=255) ggTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGaaaaa > 2:2750207/1‑150 (MQ=255) ggTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGaaaaa > 2:1514111/1‑150 (MQ=255) gTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAAc < 2:48899/150‑1 (MQ=255) ggCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAATGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCCCGTCAGGGGGGCGAAGCCTATGGAAAAACGcc < 2:335294/149‑1 (MQ=255) aGCGGCAGGGCCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGcc < 1:5617834/150‑1 (MQ=255) aGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAc < 1:4403046/150‑1 (MQ=255) aGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAc < 1:4893341/150‑1 (MQ=255) ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgc < 1:5407866/150‑1 (MQ=255) ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgc < 1:2192593/150‑1 (MQ=255) ggTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAAcgc > 1:1707829/1‑150 (MQ=255) gatggAACGGTAGACCGCCCGAGGGAGTTCACAGGGATAAACGCCGGTTATCTTTTTAGTCCTGTTGGGTTTCGCCACCTCTGTCTTGAGCGTAGATTTTTGTGATCCCCGTCAGGGCGGCTAAGCCTATGGAAAAACGCCAGCAAcgcg < 1:3520968/147‑1 (MQ=255) tgggAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCgg < 1:3005542/148‑1 (MQ=255) gaccAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCt < 1:5199093/147‑1 (MQ=255) aaCAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCtt < 2:469291/150‑1 (MQ=255) aaCAGGAGAGCGCACGAGGGAGCTCCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCtt < 1:2398364/150‑1 (MQ=255) aCAGGAGAGCGCACGAGGGGGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGTCACCTCTGACTTGAGCGTAGTTTTTTGTGATACTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCttt > 2:3045811/1‑150 (MQ=255) agagCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACg < 2:4360383/150‑1 (MQ=255) gcACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTcc > 2:5473366/1‑150 (MQ=255) cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct < 2:1625657/150‑1 (MQ=255) cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct < 2:139193/150‑1 (MQ=255) cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct < 2:5128024/150‑1 (MQ=255) cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct < 2:1062802/150‑1 (MQ=255) cACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGCCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCCCGTCAGGGGGGCAAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCct < 2:315487/150‑1 (MQ=255) aCGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCATCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctg < 1:110217/150‑1 (MQ=255) gAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggc > 1:1000411/1‑150 (MQ=255) aGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggcctttt < 2:4025360/150‑1 (MQ=255) ttCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgct < 1:2905874/150‑1 (MQ=255) tCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGGGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg > 1:312339/1‑150 (MQ=255) tCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAGACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg < 1:3984922/150‑1 (MQ=255) tCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg < 1:4176418/150‑1 (MQ=255) tCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctg > 1:4694298/1‑150 (MQ=255) ccAGGGGGAAACGCCTGGTATCTTTATAGTCGTGTCGGGTTTCCCCCCCTTTGACTTGAGTGTCCATTTTTGTGATGCTCGTCAGGGGGGCTAAGCCTATGGAAAGACGCCAGCAACGCGGCCTTTTTACGGTTActggccttttgctgg < 1:941945/150‑1 (MQ=255) ggggAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCctggccttttgctggcctt > 1:5714370/1‑150 (MQ=255) cTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGTTTTTTGTGATGCCCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACAT < 1:5516179‑M1/150‑1 (MQ=255) tATCTTTATATTCCTGTGGGGTTACGCCATCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTc < 1:370841/150‑1 (MQ=255) aTCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCCAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCt < 1:3205736/150‑1 (MQ=255) cTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCttt < 1:478713/150‑1 (MQ=255) ttATAGTCCTGTGGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTTTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc < 1:5813031/150‑1 (MQ=255) ttATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTcc < 2:3711399/150‑1 (MQ=255) ataGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTg > 2:4149170/1‑150 (MQ=255) tatgccTGTCGCCTTTCTCCACCCCTGACTTGAGCGTAGATTTTTGTGATCGGCCTCAGGGGGTCTAAGCCTATGGAAATACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGc < 1:1310583/146‑1 (MQ=255) taGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCACGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGc > 1:498823/1‑150 (MQ=255) aGTCTTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGGTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCg < 1:1195020/150‑1 (MQ=255) aGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCg > 1:3890215/1‑150 (MQ=255) aGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCg > 2:501538/1‑150 (MQ=255) tCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGtt < 1:510473/150‑1 (MQ=255) ccTGTGGGGTTTCCCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTa < 1:1506189/150‑1 (MQ=255) cTGTCGGTTTCCGCCACCTCTGATTTGAGCGTCGTTTTTTGTGAGGCCCTACAGGGGGGCCAAGCCTATGGAAATACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAt < 1:2008734/150‑1 (MQ=255) cTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTAt > 2:3254101/1‑150 (MQ=255) tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATc < 2:1746229/150‑1 (MQ=255) tGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATc > 2:4588755/1‑150 (MQ=255) gggTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCt < 2:5359450/150‑1 (MQ=255) gggTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGACCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCt > 1:1613322/1‑150 (MQ=255) ggTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTg > 2:2305459/1‑150 (MQ=255) gTTTCGCCACCTCTGACTTGCGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCtca > 1:2094358/1‑148 (MQ=255) tttCCCCCCCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCCAAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAt < 1:2688867/150‑1 (MQ=255) ttCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGAtt < 1:4290755/150‑1 (MQ=255) tCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTc > 2:2951137/1‑150 (MQ=255) cGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCt < 1:2497765/150‑1 (MQ=255) gCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCtg > 2:5576233/1‑150 (MQ=255) gCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCtg > 2:6589/1‑150 (MQ=255) ccACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCtgt > 2:774663/1‑150 (MQ=255) ctctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGAt > 2:5797883/1‑150 (MQ=255) ctGACTTGAGCGTCGATTTTTGTGCTGCTCGTCAGGGGGGCGTAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACCTGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa > 2:1284009/1‑150 (MQ=255) ctGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATaa > 2:1160778/1‑150 (MQ=255) tGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAc < 1:3317786/150‑1 (MQ=255) gACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc > 2:872810/1‑150 (MQ=255) gACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc < 2:3804560/150‑1 (MQ=255) gACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCAAAGCCTATGGAAAGACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAAcc < 1:3609113/150‑1 (MQ=255) ttGAGCGTCGATTTTTGTGGTGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATATCCGTa > 2:1864489/1‑150 (MQ=255) gAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTAtt > 2:3585902/1‑150 (MQ=255) gAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTAtt < 2:4792018/150‑1 (MQ=255) cGTCGATTTTTGTGATTCTCGTCAGGGGGGCTAATCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCTTTTTGCTGGCCTTTAGCTCACATGTTCTTTCCTGCGTTATCCCTTGATTCTGTGGATAACCGTATTAcc < 1:181475/150‑1 (MQ=255) cGATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGcc > 1:1412184/1‑150 (MQ=255) gATTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCt > 2:2197671/1‑150 (MQ=255) ttttttGTGATCCTCGTCAGGGGGGCTAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt < 1:3425458/149‑1 (MQ=255) aTTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt < 2:2413050/150‑1 (MQ=255) aTTTTTGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCtt < 1:656150/150‑1 (MQ=255) tttttGTGATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCttt > 1:4603611/1‑150 (MQ=255) gtgATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtg > 2:4250208/1‑150 (MQ=255) gtgATGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtg > 1:839453/1‑150 (MQ=255) tgATGCGCGTCAGGGGGGCCAAGCCTATGGAAACACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTtgagtga < 2:2519333/150‑1 (MQ=255) tGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCt < 2:4474979/150‑1 (MQ=255) tGCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCt > 1:2788263/1‑150 (MQ=255) tGCTCGTCAGGGGGGCAAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCt < 1:191459/150‑1 (MQ=255) gCTCGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTg < 1:2596098/150‑1 (MQ=255) cGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTa > 2:3472387/1‑150 (MQ=255) cGTCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTa > 1:4502035/1‑150 (MQ=255) tCAGGGGGGCTAAGCCTATGGAAAAACCCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATg < 1:742608/150‑1 (MQ=255) tCAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATg > 2:568781/1‑150 (MQ=255) cAGGGGGGCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATGa < 2:2849712/150‑1 (MQ=255) gCGAAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATGACACaaaa < 2:3915257/150‑1 (MQ=255) | CGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGTTATGACACAAAA > pSIJ663_pHT315_araE_xy/5330‑5624 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |