BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	1,395,470	A→G	6.3%	L186P (CTA→CCA)	U712_07140 ←	Sporulation kinase D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	1,395,470	0	A	G	6.3%	1266.4 / 63.1	413	L186P (CTA→CCA)	U712_07140	Sporulation kinase D
Reads supporting (aligned to +/- strand): ref base A (185/202); new base G (16/10); total (201/212)
Fisher's exact test for biased strand distribution p-value = 2.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 420 total aligned reads displayed.

TGACCGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACTGTC  >  CP006881/1395321‑1395619

 gACCGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAg                                                                                                                                                      <  1:3693927/150‑1 (MQ=255)

 gACCGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAg                                                                                                                                                      >  2:601487/1‑150 (MQ=255)

 gACCGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAg                                                                                                                                                      <  1:4302443/150‑1 (MQ=255)

   ccGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTa                                                                                                                                                    <  2:3988364/150‑1 (MQ=255)

   ccGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTa                                                                                                                                                    <  1:3023028/150‑1 (MQ=255)

      ggTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGt                                                                                                                                                 <  2:5699026/150‑1 (MQ=255)

          cGGATAGACTTTCATATTCCAGCTGATATCATACAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAAt                                                                                                                                             <  2:4754704/150‑1 (MQ=255)

           ggATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGTGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATg                                                                                                                                            >  2:4131634/1‑150 (MQ=255)

           ggATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATg                                                                                                                                            <  1:5501432/150‑1 (MQ=255)

            gATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGTTCCTCTGCATGGGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATga                                                                                                                                           >  1:3477978/1‑150 (MQ=255)

            gATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATga                                                                                                                                           >  2:5793581/1‑150 (MQ=255)

              tAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATgaga                                                                                                                                         >  1:941312/1‑150 (MQ=255)

                gACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGAtt                                                                                                                                       <  1:4097605/150‑1 (MQ=255)

                       atatTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCaaa                                                                                                                                <  1:3082592/150‑1 (MQ=255)

                            ccAGCTGATAACATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCCTTTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTACAAATAATc                                                                                                                           <  1:162367/150‑1 (MQ=255)

                              aGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCaa                                                                                                                         >  1:2617891/1‑150 (MQ=255)

                                cTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTGGTAAGTTAATGAGATTTTTCAAATAATCAAtt                                                                                                                       <  1:3191676/150‑1 (MQ=255)

                                cTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAAtt                                                                                                                       <  1:185607/150‑1 (MQ=255)

                                cTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAAtt                                                                                                                       <  2:5623564/150‑1 (MQ=255)

                                 tGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAAttt                                                                                                                      <  1:290441/150‑1 (MQ=255)

                                   atatCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGa                                                                                                                    <  2:3719109/150‑1 (MQ=255)

                                   atatCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGa                                                                                                                    <  2:3147568/150‑1 (MQ=255)

                                    tatCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGaa                                                                                                                   <  2:4598176/150‑1 (MQ=255)

                                    tatCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGaa                                                                                                                   <  1:4882441/150‑1 (MQ=255)

                                     atcatcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAAt                                                                                                                  <  2:332746/150‑1 (MQ=255)

                                        atcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGc                                                                                                               <  2:2764843/150‑1 (MQ=255)

                                        atcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGc                                                                                                               >  2:2245026/1‑150 (MQ=255)

                                        atcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTCCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGc                                                                                                               <  2:1048153/150‑1 (MQ=255)

                                         tcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCt                                                                                                              >  1:4439211/1‑150 (MQ=255)

                                         tcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCt                                                                                                              >  1:5631220/1‑150 (MQ=255)

                                         tcCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCt                                                                                                              <  1:1552086/150‑1 (MQ=255)

                                          ccAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTg                                                                                                             <  2:837464/150‑1 (MQ=255)

                                          ccAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTg                                                                                                             <  1:4994381/150‑1 (MQ=255)

                                           cAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGc                                                                                                            <  2:3944628/150‑1 (MQ=255)

                                            aGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCg                                                                                                           <  2:424810/150‑1 (MQ=255)

                                               tatCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACa                                                                                                        >  2:1660842/1‑150 (MQ=255)

                                               tatCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACa                                                                                                        >  2:236675/1‑150 (MQ=255)

                                                   cGCTGACAGGTTTCTGATCCTCTGCATGGGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGGGACAAGAt                                                                                                    >  1:1228981/1‑150 (MQ=255)

                                                    gCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATa                                                                                                   <  2:2937652/150‑1 (MQ=255)

                                                     cTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATaa                                                                                                  >  2:5328358/1‑150 (MQ=255)

                                                     cTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATaa                                                                                                  <  2:5044162/150‑1 (MQ=255)

                                                      tGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAAt                                                                                                 >  2:5453026/1‑150 (MQ=255)

                                                       gACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATc                                                                                                >  1:250859/1‑150 (MQ=255)

                                                          aGGATTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTGGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGt                                                                                             >  1:2326661/1‑150 (MQ=255)

                                                             tttCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGTGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTcac                                                                                          >  1:2473139/1‑150 (MQ=255)

                                                                  gATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTAc                                                                                     >  2:1735764/1‑150 (MQ=255)

                                                                     ccTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTTATGAGATTTTTCTAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGtt                                                                                  >  1:619178/1‑150 (MQ=255)

                                                                     ccTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGtt                                                                                  <  1:5052627/150‑1 (MQ=255)

                                                                            aTGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTc                                                                           >  1:3426132/1‑150 (MQ=255)

                                                                            aTGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTc                                                                           <  1:100021/150‑1 (MQ=255)

                                                                            aTGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTc                                                                           <  2:525175/150‑1 (MQ=255)

                                                                                    cctGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAg                                                                   <  1:2232439/148‑1 (MQ=255)

                                                                                    cTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAg                                                                   <  2:684308/150‑1 (MQ=255)

                                                                                     ttGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAgg                                                                  <  1:448425/150‑1 (MQ=255)

                                                                                        tCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGcac                                                               <  1:773911/150‑1 (MQ=255)

                                                                                          cGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTGGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGcacac                                                             >  1:2189536/1‑150 (MQ=255)

                                                                                           gCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGcacaca                                                            >  2:490144/1‑150 (MQ=255)

                                                                                             tCGCAATTATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCATAGTAAGTTACTGAGATTTTTCAAGTAATCAATTTGAATTGCTGTGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACaaa                                                          >  1:5740892/1‑150 (MQ=255)

                                                                                                  aaaaTCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATtgtg                                                     >  1:2032742/1‑150 (MQ=255)

                                                                                                    aaTCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGaa                                                   <  1:51470/150‑1 (MQ=255)

                                                                                                     aTCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTNCGACAAGATNATCTGTCACATTACGTTTTGAGTNCAAAACAGGCACACAAATTGNGaan                                                  <  1:4544629/150‑2 (MQ=255)

                                                                                                      tCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGaaaa                                                 <  1:3252762/150‑1 (MQ=255)

                                                                                                       cATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAAt                                                >  2:2186579/1‑150 (MQ=255)

                                                                                                        aTTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAAtt                                               >  1:5523763/1‑150 (MQ=255)

                                                                                                        aTTTTCCCGTCTTGGTTCACAACATCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAAtt                                               >  1:3951120/1‑150 (MQ=255)

                                                                                                         ttttCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTg                                              <  1:2542321/150‑1 (MQ=255)

                                                                                                         ttttCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTg                                              <  2:1383189/150‑1 (MQ=255)

                                                                                                           ttCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGc                                            >  2:5546784/1‑150 (MQ=255)

                                                                                                           ttCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGCAATTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTTCGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGc                                            <  1:2747550/150‑1 (MQ=255)

                                                                                                            tCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCt                                           >  2:1289512/1‑150 (MQ=255)

                                                                                                             cccGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTg                                          >  1:1987360/1‑150 (MQ=255)

                                                                                                              ccGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGa                                         >  1:3581511/1‑150 (MQ=255)

                                                                                                               cGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGAc                                        <  2:3857060/150‑1 (MQ=255)

                                                                                                               cGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGAc                                        <  1:5328358/150‑1 (MQ=255)

                                                                                                                gTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGAcc                                       >  1:1805160/1‑150 (MQ=255)

                                                                                                                gTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGAcc                                       <  2:4453302/150‑1 (MQ=255)

                                                                                                                  cTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAg                                     >  2:243429/1‑150 (MQ=255)

                                                                                                                      gTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAAt                                 >  2:4355498/1‑150 (MQ=255)

                                                                                                                      gTTCACAACTCAAATATAAACATCAGGGTATAGTAAGTTAATGATATTTTTCAAATAATAAATTTGATTAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAAt                                 <  1:4346204/150‑1 (MQ=255)

                                                                                                                        tCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTc                               >  1:67716/1‑150 (MQ=255)

                                                                                                                         cacaACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCt                              >  2:4047263/1‑150 (MQ=255)

                                                                                                                         cacaACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCt                              <  1:5462551/150‑1 (MQ=255)

                                                                                                                         cacaACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACANNACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCt                              >  1:1159515/1‑150 (MQ=255)

                                                                                                                           caACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGc                            >  1:1685637/1‑150 (MQ=255)

                                                                                                                            aaCTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCt                           <  1:4754403/150‑1 (MQ=255)

                                                                                                                             aCTTCAATATAAACATCAGGGCTTGGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTc                          >  2:3553335/1‑150 (MQ=255)

                                                                                                                             aCTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTc                          <  2:1997297/150‑1 (MQ=255)

                                                                                                                              cTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGTGTCCAAAACTGGCACACAAAATGTGAAAATATGCTGGCCAGTAATTCTGCTCg                         >  1:5646757/1‑150 (MQ=255)

                                                                                                                              cTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCg                         >  2:429375/1‑150 (MQ=255)

                                                                                                                               ttCAATATAAACATCAGTGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTAAGTCCAAAACAGGCACAAAAATTGTGAAAATTGGCTGATCAGGAATTCTGCTTGa                        >  1:3769860/1‑150 (MQ=255)

                                                                                                                                  aaTATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATa                     >  2:3634339/1‑150 (MQ=255)

                                                                                                                                   atatAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATaa                    <  2:4548666/150‑1 (MQ=255)

                                                                                                                                      taAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTa                 >  2:1588500/1‑150 (MQ=255)

                                                                                                                                          cATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTg             >  2:2701493/1‑150 (MQ=255)

                                                                                                                                           aTCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATACTGCTCGAATAACTATCTGa            >  2:3421260/1‑150 (MQ=255)

                                                                                                                                            tCAGGGCTTGGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGaa           <  1:1347137/150‑1 (MQ=255)

                                                                                                                                                  cTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACt     >  2:3010285/1‑150 (MQ=255)

                                                                                                                                                  cTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACt     <  1:1128257/150‑1 (MQ=255)

                                                                                                                                                     aGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACTGTc  >  1:4179714/1‑150 (MQ=255)

                                                                                                                                                     aGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGCGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACTGTc  >  2:2108942/1‑150 (MQ=255)

TGACCGGGTTCGGATAGACTTTCATATTCCAGCTGATATCATCCAGATATCCGCTGACAGGTTTCTGATCCTCTGCATGAGAGGCTTGTCCGCTCGCAAAAATCATTTTCCCGTCTTGGTTCACAACTTCAATATAAACATCAGGGCTTAGTAAGTTAATGAGATTTTTCAAATAATCAATTTGAATAGCTGCGACAAGATAATCTGTCACATTACGTTTTGAGTCCAAAACAGGCACACAAATTGTGAAAATTGGCTGACCAGTAATTCTGCTCGAATAACTATCTGAAATGACTGTC  >  CP006881/1395321‑1395619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`