BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	2,141,255	A→G	9.7%	I66I (ATT→ATC)	U712_10695 ←	KHG/KDPG aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	2,141,255	0	A	G	9.7%	1133.9 / 104.3	409	I66I (ATT→ATC)	U712_10695	KHG/KDPG aldolase
Reads supporting (aligned to +/- strand): ref base A (179/189); new base G (12/28); total (191/218)
Fisher's exact test for biased strand distribution p-value = 2.97e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 419 total aligned reads displayed.

CTCGGAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAATGACTGC  >  CP006881/2141106‑2141404

cTCGGAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCa                                                                                                                                                       <  1:4980746/150‑1 (MQ=255)

cTCGGAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCa                                                                                                                                                       >  1:627498/1‑150 (MQ=255)

 tCGGAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCaa                                                                                                                                                      >  1:2757088/1‑150 (MQ=255)

  cGGAGTCAACACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAAt                                                                                                                                                     <  1:3081498/150‑1 (MQ=255)

   ggAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAAtt                                                                                                                                                    <  1:72991/150‑1 (MQ=255)

       tCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTaaaa                                                                                                                                                <  2:2632840/150‑1 (MQ=255)

        cAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAat                                                                                                                                               <  1:3891180/150‑1 (MQ=255)

        cAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAat                                                                                                                                               >  2:4890258/1‑150 (MQ=255)

          aGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAatat                                                                                                                                             <  1:3212945/150‑1 (MQ=255)

                 ggggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCt                                                                                                                                      >  2:2236374/1‑150 (MQ=255)

                 ggggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCt                                                                                                                                      <  1:4319328/150‑1 (MQ=255)

                 ggggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCt                                                                                                                                      <  2:4359150/150‑1 (MQ=255)

                  gggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGTTCAGCTCAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCCCCAATTAAAATATCTTCCCTa                                                                                                                                     >  1:2544859/1‑150 (MQ=255)

                  gggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTa                                                                                                                                     >  2:1260323/1‑150 (MQ=255)

                   ggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTAt                                                                                                                                    >  2:5416367/1‑150 (MQ=255)

                   ggATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTAt                                                                                                                                    <  1:2423695/150‑1 (MQ=255)

                     atatAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTa                                                                                                                                  <  2:51223/150‑1 (MQ=255)

                       ataATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACg                                                                                                                                <  1:2044482/150‑1 (MQ=255)

                       ataATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTCAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCTCAGCTTGCTGCGCGCTGATGACCGTACCCGCGTCAATTAAAATATCTTCCCTATTACg                                                                                                                                <  1:524577/150‑1 (MQ=255)

                          aTGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGaa                                                                                                                             >  2:3990624/1‑150 (MQ=255)

                            gtgtCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAgg                                                                                                                           >  2:3263390/1‑150 (MQ=255)

                              gtCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGAt                                                                                                                         <  2:4716431/150‑1 (MQ=255)

                              gtCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGAt                                                                                                                         >  1:3487237/1‑150 (MQ=255)

                               tCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGAtt                                                                                                                        >  1:4455919/1‑150 (MQ=255)

                               tCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGAtt                                                                                                                        <  1:4153076/150‑1 (MQ=255)

                                cTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTc                                                                                                                       <  2:5777556/150‑1 (MQ=255)

                                  ttACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGTCCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGa                                                                                                                     >  1:5673249/1‑150 (MQ=255)

                                  ttACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGGAGGATTCGa                                                                                                                     >  1:893898/1‑150 (MQ=255)

                                   tACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGat                                                                                                                    <  2:2521471/150‑1 (MQ=255)

                                     cAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGataa                                                                                                                  <  2:3168371/150‑1 (MQ=255)

                                         agatagatGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATc                                                                                                              >  2:3888313/1‑150 (MQ=255)

                                           atagatGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTg                                                                                                            >  2:102134/1‑150 (MQ=255)

                                             agatGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGAt                                                                                                          >  1:400716/1‑150 (MQ=255)

                                               atGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGc                                                                                                        >  2:269620/1‑150 (MQ=255)

                                                  ttCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGcccc                                                                                                     <  1:4760357/150‑1 (MQ=255)

                                                   tCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCg                                                                                                    >  1:3336239/1‑150 (MQ=255)

                                                     aGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCggg                                                                                                  <  1:5534132/150‑1 (MQ=255)

                                                      gCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCgggg                                                                                                 <  2:1602808/150‑1 (MQ=255)

                                                       cAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCggggg                                                                                                >  2:196190/1‑150 (MQ=255)

                                                        aaGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGt                                                                                               >  1:1874188/1‑150 (MQ=255)

                                                         aGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTc                                                                                              >  1:3288724/1‑150 (MQ=255)

                                                          gATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCg                                                                                             >  1:3517794/1‑150 (MQ=255)

                                                            tCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGta                                                                                           >  1:1636768/1‑150 (MQ=255)

                                                             cAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGtat                                                                                          >  2:1502537/1‑150 (MQ=255)

                                                              aGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGtata                                                                                         >  2:3443896/1‑150 (MQ=255)

                                                              aGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGtata                                                                                         >  1:1440942/1‑150 (MQ=255)

                                                                cTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACg                                                                                       >  1:4813409/1‑150 (MQ=255)

                                                                cTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACg                                                                                       >  1:4564411/1‑150 (MQ=255)

                                                                  gAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTc                                                                                     <  2:2158579/150‑1 (MQ=255)

                                                                   aaaaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCa                                                                                    >  2:5531205/1‑150 (MQ=255)

                                                                   aaaaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCa                                                                                    <  2:3508936/150‑1 (MQ=255)

                                                                   aaaaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCa                                                                                    >  1:2879104/1‑150 (MQ=255)

                                                                   aaaaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCa                                                                                    >  2:4506963/1‑150 (MQ=255)

                                                                    aaaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGAAAAGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCAc                                                                                   >  1:3759699/1‑150 (MQ=255)

                                                                     aaaCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACt                                                                                  >  2:877294/1‑150 (MQ=255)

                                                                       aCCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCACAGCTTGCTGCGCTCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTc                                                                                <  1:4230197/150‑1 (MQ=255)

                                                                        cccGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCa                                                                               <  1:5769621/150‑1 (MQ=255)

                                                                        cccGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCa                                                                               <  2:1429570/150‑1 (MQ=255)

                                                                          cGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAAc                                                                             >  2:4816875/1‑150 (MQ=255)

                                                                           ggACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACt                                                                            <  2:1770592/150‑1 (MQ=255)

                                                                             aCTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACACGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGc                                                                          >  2:3362432/1‑150 (MQ=255)

                                                                              cTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCa                                                                         <  2:571522/150‑1 (MQ=255)

                                                                               tGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCAc                                                                        <  2:810566/150‑1 (MQ=255)

                                                                               tGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCAc                                                                        <  1:5366716/150‑1 (MQ=255)

                                                                                   aaTAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAAt                                                                    >  2:2972066/1‑150 (MQ=255)

                                                                                    aTAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATc                                                                   <  1:269620/150‑1 (MQ=255)

                                                                                     tAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATcc                                                                  >  1:11419/1‑150 (MQ=255)

                                                                                      aaaCTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCTCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATccc                                                                 <  2:5834222/150‑1 (MQ=255)

                                                                                       aaCTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCt                                                                <  2:1874188/150‑1 (MQ=255)

                                                                                         cTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCttt                                                              <  2:3642447/150‑1 (MQ=255)

                                                                                           gcgcgcCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTAt                                                            <  2:2467524/150‑1 (MQ=255)

                                                                                            cgcgcCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATc                                                           <  1:996550/150‑1 (MQ=255)

                                                                                             gcgcCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCt                                                          <  1:1235268/150‑1 (MQ=255)

                                                                                                ccAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCtaat                                                       >  1:4885209/1‑150 (MQ=255)

                                                                                                  aGCCTTAGCAGCTTCCCCGGCTTGCTGCGCGCTGATGATCGTACCGGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCCCTTCAACTGCGCGAATCCCTTTATCtaataa                                                     <  2:2012633/150‑1 (MQ=255)

                                                                                                  aGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCtaataa                                                     <  2:4925140/150‑1 (MQ=255)

                                                                                                         gcagcTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCa                                              <  2:4656806/150‑1 (MQ=255)

                                                                                                         gcagcTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCa                                              <  1:1004166/150‑1 (MQ=255)

                                                                                                          cagcTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCaa                                             >  1:1059586/1‑150 (MQ=255)

                                                                                                             cTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATct                                          <  2:5673249/150‑1 (MQ=255)

                                                                                                              ttCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATctg                                         >  2:2905312/1‑150 (MQ=255)

                                                                                                               tCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATctgc                                        <  2:676572/150‑1 (MQ=255)

                                                                                                                  ccAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGa                                     <  2:4262137/150‑1 (MQ=255)

                                                                                                                   cAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGAc                                    <  1:2972064/150‑1 (MQ=255)

                                                                                                                   cAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGAc                                    <  2:3124414/150‑1 (MQ=255)

                                                                                                                      cTTGCTGCGCGCTGATGACCGTACCCGCGCCGATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAgg                                 <  1:1985612/150‑1 (MQ=255)

                                                                                                                                  agatgaCCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTACTGCTTAt                     >  2:3037623/2‑150 (MQ=255)

                                                                                                                                     tgaCCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCt                  <  1:2079007/150‑1 (MQ=255)

                                                                                                                                       aCCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCttt                >  1:5072541/1‑150 (MQ=255)

                                                                                                                                        ccGTACCCGCGCCCATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTg               <  2:2074589/150‑1 (MQ=255)

                                                                                                                                          gTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGaa             >  1:129433/1‑150 (MQ=255)

                                                                                                                                          gTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGaa             >  1:2187797/1‑150 (MQ=255)

                                                                                                                                           tACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAAc            <  1:1587789/150‑1 (MQ=255)

                                                                                                                                           tACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAAc            <  1:4679037/150‑1 (MQ=255)

                                                                                                                                           tACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAAc            <  1:17413/150‑1 (MQ=255)

                                                                                                                                            aCCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGACCg           <  2:4036621/150‑1 (MQ=255)

                                                                                                                                               cgcgCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAAt        <  1:4028783/150‑1 (MQ=255)

                                                                                                                                                gcgcCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAATg       >  1:5445777/1‑150 (MQ=255)

                                                                                                                                                 cgcCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAATGa      >  1:3022965/1‑150 (MQ=255)

                                                                                                                                                  gcCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAATGAc     >  2:2595929/1‑150 (MQ=255)

CTCGGAGTCAAGACGCCGGGGATATAATGTGTCTTTACAAAAGATAGATGTTCAGCAAGATCAGCTGAAAAACCCGGACTGACAATAAACTGCGCGCCAGCCTTAGCAGCTTCCCCAGCTTGCTGCGCGCTGATGACCGTACCCGCGCCAATTAAAATATCTTCCCTATTACGGAAGGATTCGATAATATCTGATGCCCCGGGGGTCGTATACGTCACTTCAACTGCACGAATCCCTTTATCTAATAAACTCTCAATCTGCTGACAGGCCTCCTGCTTATCCTTTGAACGAATGACTGC  >  CP006881/2141106‑2141404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`