Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 2,909,199 C→T 11.2% G49E (GGA→GAA)  U712_14990 ← Biotin synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068812,909,1990CT11.2% 977.6 / 34.9 342G49E (GGA→GAA) U712_14990Biotin synthase
Reads supporting (aligned to +/- strand):  ref base C (103/192);  new base T (42/3);  total (145/197)
Fisher's exact test for biased strand distribution p-value = 2.23e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

Only 100 of 352 total aligned reads displayed.

GCTTCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATTCTC  >  CP006881/2909050‑2909348
                                                                                                                                                     |                                                                                                                                                     
  ttCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCg                                                                                                                                                     <  2:4048374/150‑1 (MQ=255)
      cgcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTTCGGGCAGAGGCCGGGTTTGGCATTCTTTATCATATTGAGCTTTACTTTTTTTTCCTaaa                                                                                                                                                 >  1:1138077/1‑150 (MQ=255)
       gcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTaaaa                                                                                                                                                <  1:5345131/150‑1 (MQ=255)
       gcCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTaaaa                                                                                                                                                <  2:1531284/150‑1 (MQ=255)
        ccTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTTCGGGCAGAGCCCGGGTTTCGCATTCATAATCATATTTTGCTTTACTTTTTTTCCTTaaaaa                                                                                                                                               >  2:4705703/1‑149 (MQ=255)
         cTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTTGCATTAATAATTATATTTAGCTTTACTTTTTTTTCCTAAAAgt                                                                                                                                              >  2:5336450/1‑150 (MQ=255)
          ttCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAgtg                                                                                                                                             <  2:1341230/150‑1 (MQ=255)
            cAAGCAGCGTTTCCTTACTCACCATCCGGTAAGACTCAATCGGCCCTTTCGAAATCCCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGtt                                                                                                                                           <  2:5677733/150‑1 (MQ=255)
               gcagcGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGGAAAGGCCGGATTTCGCAATCATAATTATATTTAGCTTTACTTTTTTTTCCTAAAAATGTTTTc                                                                                                                                        >  1:2501939/1‑150 (MQ=255)
                    gTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTTCGGGCAGAGCCCGGGTTTCGCATTCATAAACATATTGTGCTTTACTTTTTTTTCGTAAAAATGtttttttgtt                                                                                                                                   >  2:1049802/1‑144 (MQ=255)
                     tttCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGAtgt                                                                                                                                  <  2:2711406/150‑1 (MQ=255)
                     tttCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGAtgt                                                                                                                                  <  2:1968008/150‑1 (MQ=255)
                       tCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGa                                                                                                                                <  2:1017129/150‑1 (MQ=255)
                        ccTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGGCCCGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCCTTAAAGTGTTTTCTTATTTGaa                                                                                                                               >  2:264684/1‑150 (MQ=255)
                           tATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAg                                                                                                                            <  2:487363/150‑1 (MQ=255)
                           tATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAg                                                                                                                            <  2:4108391/150‑1 (MQ=255)
                           tATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAg                                                                                                                            <  1:3393758/150‑1 (MQ=255)
                            nttCNCCATCCGGTAAGACTCAATCNGCGCTTTCGAAATCGCAGACTGTNAACAATAGCNGCAGTTTTTCGGGCAGAGCCCGGGTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCCTAAAAGTGTTTTCTTATGTGaaaaac                                                                                                                           >  1:4529428/2‑148 (MQ=255)
                             ttCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGcc                                                                                                                          <  2:4084585/150‑1 (MQ=255)
                              tcatcaTCCGCTAAGACTTAATGGGTGCTTTGGAAACCGCAGACAGTGAACAATAGTCGGAGTTATCCGGGCAGAGCTCGAATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTATGTAAAAGTGTTTTCTGATGTGAAAAgtac                                                                                                                         <  1:3666269/150‑4 (MQ=255)
                              tCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGATCAATAGCCGCAGTTTTCCGGGCAGAGCCCGAATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGccc                                                                                                                         <  1:2233955/150‑1 (MQ=255)
                              tCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGccc                                                                                                                         <  2:3671634/150‑1 (MQ=255)
                               cACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGcccc                                                                                                                        <  2:4384666/150‑1 (MQ=255)
                                aCCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCg                                                                                                                       <  1:4429028/150‑1 (MQ=255)
                                aCCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCg                                                                                                                       <  1:1782787/150‑1 (MQ=255)
                                  cATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTg                                                                                                                     <  2:3885894/150‑1 (MQ=255)
                                             aCTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCCGGGCCCCGATTTCGCATTCATAATCATAATGAGCTTTACTTTTTTTCCCTAAAAAGGTTTTTTTGTGTGAAAAGCCCCGTGCATTAATAGCa                                                                                                          >  2:2837325/1‑150 (MQ=255)
                                              cTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCGGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCaa                                                                                                         <  1:4177239/150‑1 (MQ=255)
                                              cTCAATCGGCGCTCGCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATATTCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATATCaa                                                                                                         <  2:830386/150‑1 (MQ=255)
                                                 aaTCGGCTCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAat                                                                                                      <  2:5441976/150‑1 (MQ=255)
                                                       cgcTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtc                                                                                                <  2:1748682/150‑1 (MQ=255)
                                                        gcTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGGGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAAAATCTtca                                                                                               >  2:2370953/1‑150 (MQ=255)
                                                         cTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtcat                                                                                              <  1:4823580/150‑1 (MQ=255)
                                                           ttCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTtcatca                                                                                            <  2:2121133/150‑1 (MQ=255)
                                                            tCGAAATCGCAGACTGTGAACAATAGTCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAg                                                                                           <  1:151963/150‑1 (MQ=255)
                                                                  tCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCGCGANTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAAt                                                                                     <  1:5035615/150‑1 (MQ=255)
                                                                  tCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAAt                                                                                     <  1:4419949/150‑1 (MQ=255)
                                                                  tCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAAt                                                                                     <  2:2219613/150‑1 (MQ=255)
                                                                  tCGCAGACTGTGAACAATAGACGCAGTTTACCGAGCAGAGCACGGATTTCTCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAAt                                                                                     <  1:3526160/150‑1 (MQ=255)
                                                                    gCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGGTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAACCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATgg                                                                                   >  1:208441/1‑149 (MQ=255)
                                                                    gCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTTCGTAAAAATGTTTTCTGTTGTGTAAAGCCCCCTGCATTAATAGCAAAATATCTTCATCAGGACAAAGt                                                                                   >  2:72420/1‑150 (MQ=255)
                                                                     cAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTa                                                                                  <  1:2405526/150‑1 (MQ=255)
                                                                     cAGACTGTGAACAATACCCGCAGTTTTCCGGGCAGAGCCCTGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTa                                                                                  <  1:3592538/150‑1 (MQ=255)
                                                                       gACTGTGAACAATAGCCGCAGTTTTCCGGGGAGAGACCGGATTTCGCATTCATAATCATATTGTGCTTTACTTTTTTTTCGTAAAAGTGTTTTCTTTTGTGGAAAAGCCCGTGGATTAATAACAAAAAAATTTTATCAGGACaaaagaat                                                                                >  2:1215899/1‑144 (MQ=255)
                                                                       gACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGTTGTGGAAAGCCCCGTGCATTAATAGCAAAAAATCTTCATCAGGACAATGTAat                                                                                >  1:3864156/1‑150 (MQ=255)
                                                                          tgtgAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATAtt                                                                             <  1:4683427/150‑1 (MQ=255)
                                                                              aaCAATAGCCGCAGTTTTCGGGGCAGAGCCCTGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGaaa                                                                         <  2:3521471/150‑1 (MQ=255)
                                                                              aaCAATAGCCGCAGTTTTCCGGGCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGaaa                                                                         <  1:284577/150‑1 (MQ=255)
                                                                               aCAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAg                                                                        <  1:2463076/150‑1 (MQ=255)
                                                                                 aaTAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAgcg                                                                      <  1:4801638/150‑1 (MQ=255)
                                                                                     gCCGCAGTTTTCCGGGCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCTGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCctc                                                                  <  1:804126/150‑1 (MQ=255)
                                                                                      ccGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCctct                                                                 <  2:4770922/150‑1 (MQ=255)
                                                                                       cGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCtt                                                                <  2:884428/150‑1 (MQ=255)
                                                                                       cGCAGTTTTCCGGGCAGAGCCCGCATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCtt                                                                <  1:2737468/150‑1 (MQ=255)
                                                                                        gCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTc                                                               <  2:3785162/150‑1 (MQ=255)
                                                                                        gCAGTTTTCCGGGCAGAGCCCAGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAGGCGCCTCTTc                                                               <  2:5568489/150‑1 (MQ=255)
                                                                                          aGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCgt                                                             >  1:5770210/1‑150 (MQ=255)
                                                                                                ccGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCgtcagtca                                                       >  2:2904845/1‑150 (MQ=255)
                                                                                                 cGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCAc                                                      >  2:552161/1‑150 (MQ=255)
                                                                                                  gggCAGAGCCCGTATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACt                                                     <  1:1690509/150‑1 (MQ=255)
                                                                                                  gggCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACt                                                     >  1:398958/1‑150 (MQ=255)
                                                                                                     cAGGGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAGGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGGCACTTCt                                                  >  1:3509841/1‑150 (MQ=255)
                                                                                                     cAGAGTCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCt                                                  <  1:4581790/150‑1 (MQ=255)
                                                                                                     cAGAGCCCGTATTACGCATCCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAATGCGCCTCTTCGTCAGACACTTCt                                                  <  2:2045215/150‑1 (MQ=255)
                                                                                                     cAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCt                                                  <  2:609930/150‑1 (MQ=255)
                                                                                                      agagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTg                                                 >  1:5830739/1‑150 (MQ=255)
                                                                                                      agagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTg                                                 >  2:5193377/1‑150 (MQ=255)
                                                                                                       gagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGc                                                <  1:264684/150‑1 (MQ=255)
                                                                                                       gagCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGc                                                >  1:4284807/1‑150 (MQ=255)
                                                                                                          cccGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCATCATCAGGACAATGTAATATTGATAGCGCCTCTTCGTCAGTCACTTCTGCTcc                                             >  2:5806579/1‑150 (MQ=255)
                                                                                                            cGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccag                                           >  1:3041380/1‑150 (MQ=255)
                                                                                                               aTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccagcca                                        <  2:2465453/150‑1 (MQ=255)
                                                                                                               aTTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTccagcca                                        <  2:4572915/150‑1 (MQ=255)
                                                                                                                   cGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCAc                                    >  1:641734/1‑150 (MQ=255)
                                                                                                                    gCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCAcc                                   >  1:5267391/1‑150 (MQ=255)
                                                                                                                        tCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGt                               <  1:552161/150‑1 (MQ=255)
                                                                                                                         cATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTc                              >  2:5408002/1‑150 (MQ=255)
                                                                                                                         cATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTc                              <  1:5384944/150‑1 (MQ=255)
                                                                                                                          aTAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCt                             >  1:3293047/1‑150 (MQ=255)
                                                                                                                          aTAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCt                             <  1:149887/150‑1 (MQ=255)
                                                                                                                            aaTCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGc                           <  1:2013298/150‑1 (MQ=255)
                                                                                                                             aTCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCTCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCg                          <  1:5129510/150‑1 (MQ=255)
                                                                                                                               cATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAg                        >  1:4892967/1‑150 (MQ=255)
                                                                                                                                atatTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAgg                       >  1:1621305/1‑149 (MQ=255)
                                                                                                                                  atTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtc                     >  2:5005915/1‑150 (MQ=255)
                                                                                                                                   ttGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcc                    >  1:2565457/1‑150 (MQ=255)
                                                                                                                                    tGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtcca                   >  1:4100886/1‑150 (MQ=255)
                                                                                                                                     gAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccat                  <  1:4705703/150‑1 (MQ=255)
                                                                                                                                     gAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccat                  >  2:1867437/1‑150 (MQ=255)
                                                                                                                                      aGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCCGCCAGCACCCGGTCTGCGAGTtccatc                 >  2:5123443/1‑150 (MQ=255)
                                                                                                                                      aGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccatc                 >  2:2982203/1‑150 (MQ=255)
                                                                                                                                       gCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccatcc                <  2:400590/150‑1 (MQ=255)
                                                                                                                                        cTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGGAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGGCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTtccatcca               >  1:1233015/1‑150 (MQ=255)
                                                                                                                                           tACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTg            <  1:5104201/150‑1 (MQ=255)
                                                                                                                                             cTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGat          <  2:1630634/150‑1 (MQ=255)
                                                                                                                                               ttttttCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGattc        >  2:4210233/1‑150 (MQ=255)
                                                                                                                                               ttttttCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGattc        <  2:3185198/150‑1 (MQ=255)
                                                                                                                                               ttttttCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAACACCCGATCTGCGAGTTCCATCCATTGattc        >  2:4490023/1‑150 (MQ=255)
                                                                                                                                                  tttCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGattcatt     <  2:1135556/150‑1 (MQ=255)
                                                                                                                                                     ccGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATtctc  >  2:39619/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCTTCGCGCCTTCAAGCAGCGTTTCCTTATTCACCATCCGGTAAGACTCAATCGGCGCTTTCGAAATCGCAGACTGTGAACAATAGCCGCAGTTTTCCGGGCAGAGCCCGGATTTCGCATTCATAATCATATTGAGCTTTACTTTTTTTCCGTAAAAGTGTTTTCTGATGTGAAAAGCCCCGTGCATTAATAGCAAAATATCTTCATCAGGACAATGTAATATTGAAAGCGCCTCTTCGTCAGTCACTTCTGCTCCAGCCAGCACCCGGTCTGCGAGTTCCATCCATTGATTCATTCTC  >  CP006881/2909050‑2909348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑