Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 3,581,984 C→G 7.5% intergenic (+18/+3) U712_18395 → / ← U712_18400 putative cardiolipin synthase 2/HTH‑type transcriptional activator mta

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0068813,581,9840CG 7.5% 880.2 / 7.0 304intergenic (+18/+3)U712_18395/U712_18400putative cardiolipin synthase 2/HTH‑type transcriptional activator mta
Reads supporting (aligned to +/- strand):  ref base C (71/196);  new base G (33/2);  total (105/199)
Fisher's exact test for biased strand distribution p-value = 3.53e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 311 total aligned reads displayed.

AAAAAACTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGTCGAG  >  CP006881/3581835‑3582133
                                                                                                                                                             |                                                                                                                                                          
 aaaaaCTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑cc                                                                                                                                                           <  1:2976337/150‑1 (MQ=255)
   aaaCTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGTTCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑tt                                                                                                                                                        >  1:4257658/1‑150 (MQ=255)
    aaCTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑GC‑ttt                                                                                                                                                       >  2:411794/1‑150 (MQ=255)
      cTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑c                                                                                                                                                    <  2:3506491/150‑1 (MQ=255)
       tGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑Cg                                                                                                                                                   <  1:5786220/150‑1 (MQ=255)
        ggTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑CG‑GC‑TTTatcg                                                                                                                                                   >  2:5201704/1‑147 (MQ=255)
          tttCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑cc                                                                                                                                               <  1:5067239/150‑1 (MQ=255)
          tttCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑cc                                                                                                                                               <  1:1026941/150‑1 (MQ=255)
            tCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑gg                                                                                                                                            <  2:90424/150‑1 (MQ=255)
             cAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑ggg                                                                                                                                           <  1:807956/150‑1 (MQ=255)
             cAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATCAGGAGCATCTCCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑ggg                                                                                                                                           <  2:3578020/150‑1 (MQ=255)
              aaCTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCCAC‑G‑GG‑GC‑TTTT‑GGG‑CC‑ggg                                                                                                                                           >  1:3335474/1‑150 (MQ=255)
              aaCTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑gggg                                                                                                                                          <  2:4233562/150‑1 (MQ=255)
              aaCTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑gggg                                                                                                                                          <  2:5546671/150‑1 (MQ=255)
              aaCTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑CTTT‑CGG‑GC‑cggg                                                                                                                                          >  2:1846136/1‑150 (MQ=255)
                  tttAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GGCCC‑TTTC‑GGG‑CC‑GGGGGtt                                                                                                                                       >  2:305334/1‑150 (MQ=255)
                   taaaaGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATCCGAAAAAAAGAACCCGT‑CTCGGGG‑CTGTTGCCGCCGATCTTATAAGATGCGAAAC‑CCC‑C‑G‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGtttt                                                                                                                                     <  2:1747018/148‑1 (MQ=255)
                    tAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑GG‑CTTA‑TCG‑CC‑GGGGGgttgc                                                                                                                                    >  1:2570800/1‑148 (MQ=255)
                       agaagaTTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑CC‑TTTA‑CGG‑CC‑CGGGGTTTTTCTg                                                                                                                                 >  2:4516962/1‑150 (MQ=255)
                         aagtTTTGCTAGTACCAAGAAAATCCCCTTATGAGGAGTATCTGCAGCCCCGTTT‑GTGGATTCAAAAAAAAGAACCCGC‑CCCGGGG‑CTGTTGCCCCCGATTTTAAAAGATGCGAAAC‑CCC‑C‑G‑GG‑CC‑TTTA‑GGG‑CC‑GGGGGTTTTCCTGAt                                                                                                                               <  2:2053077/150‑1 (MQ=255)
                             tttGCTNTTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGCCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GAGGGTTTTCCTGATGGTc                                                                                                                           <  1:3703833/150‑1 (MQ=255)
                             tttGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCA‑C‑G‑GC‑GC‑TTTA‑CGGCCC‑GGGGGTTTTCTTgtgggg                                                                                                                            >  2:2373859/1‑145 (MQ=255)
                                gCTAGTATCCAGAAAATTCACCTATGAGGAGTACCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTcaca                                                                                                                        <  1:5316972/150‑1 (MQ=255)
                                 cTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAACCCC‑C‑C‑GG‑CC‑TTT‑‑CGG‑CC‑CGGGGGTTTTCTTATGGTCACAg                                                                                                                       >  2:5327394/1‑150 (MQ=255)
                                 atAGTATCAAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCGGTT‑GTGGATTCGAAAAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAg                                                                                                                       <  1:5246406/149‑1 (MQ=255)
                                  tAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAACCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑t                                                                                                                     <  2:2217495/150‑1 (MQ=255)
                                  tAGTAACCAAAAAATTCCCCTATGGGGAATATCTGCCGGCCCCGTT‑GTGGATTCGAAATAAAAAAACCGTCCTGGGGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGTGGTTTTCCTGATGGTCACAg                                                                                                                       <  2:1154224/150‑1 (MQ=255)
                                     tATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTATCGG‑CG‑CGGGGTTTT‑CTGTTTGTcacaagaaa                                                                                                                   >  1:311493/1‑145 (MQ=255)
                                      aTCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑CC‑TTTT‑CTG‑CC‑GGGGGGTTTTCTTATGGTCACAA‑TAAat                                                                                                                 >  2:3769715/1‑150 (MQ=255)
                                        ccAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTT‑CCG‑CC‑GGGGGGTTTTCTGGTGGTGACAG‑GAAatga                                                                                                               >  2:3080320/1‑150 (MQ=255)
                                           gAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑T‑C‑GG‑CC‑TTTT‑TGG‑CCGGGGGGTTTTCTTGA‑GGGGACAG‑AAAAagatga                                                                                                            >  2:2770148/1‑150 (MQ=255)
                                           gAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAAtgatga                                                                                                            <  1:1467912/150‑1 (MQ=255)
                                             aattccACCTATGAGGAGTATCTGCACCGCCGGTT‑GTGGATTCGAATAAAAGAATCCGT‑CCCGCGG‑CTGTTGCCGCCGATCTTATAAGATGCGAAAA‑CCC‑C‑C‑GG‑CC‑TTTC‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGAtt                                                                                                          <  2:4103998/145‑1 (MQ=255)
                                             aaaTTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGAtt                                                                                                          <  2:4298606/150‑1 (MQ=255)
                                                  ccccTAGGAGGATTACCTGCAGCGCGCGTG‑GTGAATCGGAATAAAAGAATCCGT‑CCCGCGC‑CTGTTGACGCCGATCTTATAAGATGCGTAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GCGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGccac                                                                                                     <  2:2987994/148‑3 (MQ=255)
                                                  cACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑CTTT‑CGG‑CC‑CGGGGGTTTCCTTTTGGGCACAG‑TAAAAGATGGTTGGCTc                                                                                                     >  2:4696548/1‑150 (MQ=255)
                                                   aCCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCCAC‑G‑GG‑CC‑TTTC‑GGG‑CC‑GGGGGTTTTCTTG‑TGGGGACAA‑GAAAAGATGATTGGCTcc                                                                                                    >  2:3126280/1‑150 (MQ=255)
                                                   aCCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑TTTA‑CGG‑CC‑GGGGGTTTTTCTGGTGGTGACAA‑GAAATGGTGGTTGTCTcc                                                                                                    >  2:1902893/1‑150 (MQ=255)
                                                    ccTATGAGGAGTATCTGCAGCGCCCTTT‑TTGGATCGGAAAAAAAGAACCCGT‑CTCGCGG‑CTGTTGTCGCGGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGCTGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTccc                                                                                                   <  1:5171021/150‑1 (MQ=255)
                                                    ccTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTccc                                                                                                   <  2:3163372/150‑1 (MQ=255)
                                                    acTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGC‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGC‑CC‑TGAAGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTccc                                                                                                   <  1:5138152/149‑1 (MQ=255)
                                                       aTGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑CC‑TTTT‑CGG‑CC‑GGGGGGTTTTCTGATGGTCACAG‑TAAAAAATAATTGGCTCCCCCa                                                                                                >  2:4951682/1‑150 (MQ=255)
                                                       aTGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑CG‑GC‑TTTA‑CGG‑GT‑GGGGGGGTTTCTTGTGGTGGCAG‑TAAAAGAAGGTTGGCTcccccc                                                                                                >  1:5403916/1‑147 (MQ=255)
                                                             agTATCTGCAG‑GCCCGTTGGTGGATTGGAATAAAAGAACCCGT‑CTCGGGG‑CTGTTGCCCCGGATCTTATAAGATGCGTAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAg                                                                                          <  2:1367238/150‑1 (MQ=255)
                                                              gTATCTGCAGCGCCCGTG‑GTGGATTCGAATAAAAGAATCCGT‑CACGCGG‑CAGTTGTCGCCGATCTTATAAGATGCGTAAT‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GAGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGc                                                                                         <  2:5024542/150‑1 (MQ=255)
                                                               tATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑CTTT‑CGG‑CC‑GGGGGGTTTTCTGATGGTGACAA‑GAAATGTAGACTGGCTCCCGCCAAAAAGCg                                                                                        >  2:2510398/1‑150 (MQ=255)
                                                                aTCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCgg                                                                                       <  2:2613362/150‑1 (MQ=255)
                                                                       gtccgcGTT‑GGGGATTAAAATAAAAGTACCCGT‑CGGGCGC‑CTTTGGGCGCCGTTATTATAAGGGGCGTACC‑CCC‑C‑G‑GG‑CC‑TTTC‑GGG‑CC‑GTGTGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGAcc                                                                                <  1:1377773/145‑1 (MQ=255)
                                                                        ccccggTT‑GTGGATTCGAATAAAAGATTCCTT‑CTCACGG‑CTGTTCTCGCCGATCTTATAAGTTCCGTAAA‑CCC‑C‑T‑GG‑CC‑TTAC‑CGG‑CC‑CCGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTTCCGCAAAAGAGCGGCAAGACCt                                                                               <  1:1864429/145‑1 (MQ=255)
                                                                         gcCCGTT‑GTGGATTCGAATAAAAGAACCCGC‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑G‑GG‑CC‑TTTA‑CGG‑CC‑GTGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCtt                                                                              <  2:1246943/150‑1 (MQ=255)
                                                                            cGTT‑GTGGATCCAAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTC‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTtctc                                                                           <  1:213113/150‑1 (MQ=255)
                                                                             gTT‑GGGGTTTCAATTAAAACATTCCGC‑CCCGCGG‑CTGTTGTGGCGGACTTTATAAGATCCTTAAA‑CCC‑C‑C‑GG‑CC‑TTTT‑CGG‑CC‑GTGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTcc                                                                          <  2:5813560/150‑1 (MQ=255)
                                                                               tgttgGACTCAAAAAAAAGAATCCCT‑CCCGCGGTCTGTTGTCGCCGTTTTTATAAGTTG‑GAAAC‑CCCGC‑C‑GG‑CT‑TTCC‑CGG‑CC‑TTGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTcc                                                                          <  1:1825263/147‑1 (MQ=255)
                                                                                 gtgGATTCAAAAAAAAGAACCCGC‑CCCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTC‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCata                                                                       <  1:5442794/150‑1 (MQ=255)
                                                                                  tgGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑GGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCatat                                                                      <  1:2379895/150‑1 (MQ=255)
                                                                                   ggATTAGAATAAAAGAACCCGC‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATatt                                                                     <  2:1528228/150‑1 (MQ=255)
                                                                                    gATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑CTTT‑CGG‑CC‑GGGGGGTTTTCTTGTGGTGACAG‑TAAAAGATGATTGGCTTCCCCCAAAAAGAGGGAAGAACTTCTTCCTattt                                                                    >  2:5082765/1‑149 (MQ=255)
                                                                                       tCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GGCCC‑TTTA‑CGC‑CC‑GGGGGTTTTCTTG‑TTGTGAAAGAAAAATAATGATGTCTTCCCCCAAAAAAGAGCAAAAAACTTTTCCATattttt                                                                  >  1:466572/1‑147 (MQ=255)
                                                                                         gAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑CAG‑GG‑CT‑TTTA‑CGC‑CC‑GGGGGTTTT‑CTGGTGGTTAAAC‑TAAAAGTAGATTTCCCCCCGCAAAAAAAAGGGAAAACCTTTTCCCTatttagtta                                                               >  1:877576/1‑144 (MQ=255)
                                                                                         gAATAAAAGAATCCGC‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATattgattga                                                               <  2:3296720/150‑1 (MQ=255)
                                                                                          aaTAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGACCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATattgattgat                                                              <  1:77509/150‑1 (MQ=255)
                                                                                           aTAAAAGAATCCGC‑CTCGCGG‑CTGTTCTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATattgattgatt                                                             <  1:3170466/150‑1 (MQ=255)
                                                                                            tAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATattgattgattg                                                            <  2:4032/150‑1 (MQ=255)
                                                                                             aaaaGAATCCGT‑CCCGCGG‑CTGTTGTCCCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGCGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATAttgattgattga                                                           <  1:4172310/150‑1 (MQ=255)
                                                                                               gaacaTCCGC‑CTCGCGC‑CTGTTGTCGGCGATCTTATAAAGTGGGTAAA‑CCC‑A‑G‑GG‑CC‑TTTA‑GGG‑CC‑TGCAGTTTTACTGATGGTCACAG‑TAAATGATGATTGCCTTCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAAt                                                         <  1:3960574/146‑1 (MQ=255)
                                                                                               aaaaaTCCGT‑CTCGCGG‑CTGTTGCCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GC‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAAt                                                         <  2:5757914/147‑1 (MQ=255)
                                                                                               aaGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAAt                                                         <  2:417704/150‑1 (MQ=255)
                                                                                                aGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATc                                                        <  2:4354739/150‑1 (MQ=255)
                                                                                                 gAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTT‑CCG‑CC‑GGGGGGTTTCCTTATGGTCACAG‑GAAAAAATGATTGCCTCCCCCCAAAAAGAGGCAAGACCTTCTCCATAATGATTTTTTGTATCg                                                       >  2:250188/1‑150 (MQ=255)
                                                                                                   aTCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑GC‑TTTT‑TGG‑CC‑GGGGGGTTTTCTTTTGGTCACAG‑TAAATAATGATTGCCTCCCCCAAAAAAACGGCAAGAGCCTCTTCATAATGATTGATTGAATCGGt                                                     >  2:1595979/1‑150 (MQ=255)
                                                                                                   aTCCGC‑CTCGCGG‑CTGTTGTCCCCGATTTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGTGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGt                                                     <  2:1326253/150‑1 (MQ=255)
                                                                                                     ccGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTC‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTaa                                                   <  1:1708830/150‑1 (MQ=255)
                                                                                                       gT‑CCCGGGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAC‑CCC‑C‑C‑GC‑CC‑TTTC‑CGG‑CC‑GAGTGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAAt                                                 <  1:5630553/150‑1 (MQ=255)
                                                                                                       ct‑ctcGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAC‑CCC‑G‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAAt                                                 <  1:1234384/149‑1 (MQ=255)
                                                                                                        t‑ctcGGGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAC‑CCC‑C‑G‑GG‑CC‑TTTA‑CGG‑CC‑GAGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATc                                                <  1:4316702/150‑1 (MQ=255)
                                                                                                        t‑ctcGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATc                                                <  2:607007/150‑1 (MQ=255)
                                                                                                         tcccgggg‑CTGTTCTCGCCGATTTTAAAAATTGCGAAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑GGG‑CC‑GTGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATc                                                <  1:2895848/144‑1 (MQ=255)
                                                                                                         cctcGCGG‑CTGTTGACGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATc                                                <  2:390488/149‑1 (MQ=255)
                                                                                                          ctcGCGG‑TGGTTTTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCg                                               <  2:5412931/150‑1 (MQ=255)
                                                                                                            cgcgG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑A‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTTCTGATGGTTAAAG‑TAAATGATGATTGACTCCCCCAAAAAAAAGGCAAGACCCTCTCCATATTTATTGATTGAATCGGTaaaaaact                                             >  1:2041868/1‑145 (MQ=255)
                                                                                                             gcgG‑CTTTTGTCGCCGTTCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGC‑CG‑TGGTGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTc                                            <  1:375817/150‑1 (MQ=255)
                                                                                                              cgG‑TTGTTGCCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCg                                           <  2:2702824/150‑1 (MQ=255)
                                                                                                               gg‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAGGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGt                                          <  2:81889/150‑1 (MQ=255)
                                                                                                                  cTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑CGGG‑CC‑TTTC‑GGC‑CC‑GGGGGTTTTCC‑GGTGGTCACAA‑GAAAAGATGAATTCCTCCCCCCAAAAAACGGCAAGACCTTCTTCCTATTGATTTTTTGTTTCGGTGAAAGGCTCCTCa                                        >  2:3844669/1‑150 (MQ=255)
                                                                                                                  cTGTTGTCGCCAATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CT‑TTTA‑CGG‑CC‑GGTGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCa                                        <  1:4516962/150‑1 (MQ=255)
                                                                                                                      tgtCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑CTTA‑CGG‑CC‑GGGGGTTTTCCTGGTGGTCACAA‑TAAATGATGGTTGCCTCCCCCCAAAAAGCGGGAAGACCTTCTCCACAATGATTGATTGGATTGGTGAAACGCTCGTCAGTgg                                    >  1:4928884/1‑149 (MQ=255)
                                                                                                                         cGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGatat                                 <  2:4871659/150‑1 (MQ=255)
                                                                                                                              aTCTTATAAGATGCGTAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACt                            <  2:4451681/150‑1 (MQ=255)
                                                                                                                                   ataAGATGCGTAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GAGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTcccc                       <  1:1780104/150‑1 (MQ=255)
                                                                                                                                       gATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGt                   <  1:4696548/150‑1 (MQ=255)
                                                                                                                                        aTGCGTAAC‑CCC‑C‑C‑GG‑CC‑TTTA‑GGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTc                  <  2:3354918/150‑1 (MQ=255)
                                                                                                                                         gggttAAC‑CCC‑C‑C‑GT‑CC‑TTTA‑GGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTcc                 <  1:914583/146‑1 (MQ=255)
                                                                                                                                          gCGTACA‑CCC‑C‑C‑GC‑CC‑TTTG‑CGC‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCct                <  2:1952216/150‑1 (MQ=255)
                                                                                                                                            gTAAA‑CCC‑C‑C‑GC‑GC‑TTTG‑CGG‑CC‑GTTGGTTTTCCTGATCGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCctct              <  1:4951425/150‑1 (MQ=255)
                                                                                                                                                   cc‑c‑g‑GG‑CC‑TTTA‑GGG‑CC‑GGGTGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAAt        <  2:1646953/150‑1 (MQ=255)
                                                                                                                                                   cc‑c‑c‑GG‑CCTTTTA‑CGG‑CC‑GTGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGaaaa         <  1:2508308/150‑1 (MQ=255)
                                                                                                                                                      c‑c‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGt      <  2:5451513/150‑1 (MQ=255)
                                                                                                                                                        c‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGTc     <  2:2947530/150‑1 (MQ=255)
                                                                                                                                                          gg‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGTCg    >  1:2721171/1‑150 (MQ=255)
                                                                                                                                                          gg‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGTCg    <  2:5638452/150‑1 (MQ=255)
                                                                                                                                                             |                                                                                                                                                          
AAAAAACTGGTTTCAACTTTTAAAGAAGATTTGCTAGTATCCAGAAAATTCACCTATGAGGAGTATCTGCAGCGCCCGTT‑GTGGATTCGAATAAAAGAATCCGT‑CTCGCGG‑CTGTTGTCGCCGATCTTATAAGATGCGTAAA‑CCC‑C‑C‑GG‑CC‑TTTA‑CGG‑CC‑GGGGGTTTTCCTGATGGTCACAG‑TAAATGATGATTGCCTCCCGCAAAAAAGCGGCAAGACCTTCTCCATATTGATTGATTGAATCGGTAAATCGCTCGTCAGTGATATACACTTCCCCGAGTCCTCTGAAAATGTCGAG  >  CP006881/3581835‑3582133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑