Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP006881 421,277 A→G 8.2% A392A (GCA→GCG)  U712_01915 → Spore germination protein KA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP006881421,2770AG 8.2% 1154.8 / 82.6 401A392A (GCA→GCG) U712_01915Spore germination protein KA
Reads supporting (aligned to +/- strand):  ref base A (191/177);  new base G (14/19);  total (205/196)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

Only 100 of 407 total aligned reads displayed.

GAAATGATACCGACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCTTATTCG  >  CP006881/421128‑421426
                                                                                                                                                     |                                                                                                                                                     
      aTACCGACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGGGGCGCTGACGATGGAGGTTGCTTGTGAGATTCTGGGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCa                                                                                                                                                 >  2:1322029/1‑150 (MQ=255)
       tACCGACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCaa                                                                                                                                                >  2:3957027/1‑150 (MQ=255)
         ccGACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATc                                                                                                                                              <  2:486611/150‑1 (MQ=255)
           gACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGt                                                                                                                                            >  1:2961479/1‑150 (MQ=255)
           gACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGt                                                                                                                                            >  2:2580575/1‑150 (MQ=255)
           gACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGt                                                                                                                                            <  1:2244039/150‑1 (MQ=255)
            aCTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGGGGCGCTGACGATGGAGGTTGCTTTTGGGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGAGTGTCGGCTCGGCAGTGTCAATccga                                                                                                                                           >  2:5291748/1‑147 (MQ=255)
            aCTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTa                                                                                                                                           >  2:4824759/1‑150 (MQ=255)
                aGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGgcg                                                                                                                                       <  1:5645279/150‑1 (MQ=255)
                aGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGgcg                                                                                                                                       >  2:3874496/1‑150 (MQ=255)
                  cTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGgcgcg                                                                                                                                     <  2:1112838/150‑1 (MQ=255)
                    gTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCt                                                                                                                                   <  2:4002919/150‑1 (MQ=255)
                    gTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCt                                                                                                                                   <  1:1878960/150‑1 (MQ=255)
                    gTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCt                                                                                                                                   <  1:3334109/150‑1 (MQ=255)
                     ttAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGCGGCTNTCTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTc                                                                                                                                  <  1:3762234/150‑1 (MQ=255)
                       aGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTCAGATTCTGAGGGAAGGGAGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCCCGCTCGt                                                                                                                                >  1:1545450/1‑150 (MQ=255)
                        gTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTa                                                                                                                               >  1:1683494/1‑150 (MQ=255)
                           gTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGAGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCTGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATc                                                                                                                            >  1:5730161/1‑150 (MQ=255)
                            tCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCg                                                                                                                           >  1:517043/1‑150 (MQ=255)
                             cATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGGCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCgg                                                                                                                          >  2:5183531/1‑150 (MQ=255)
                               ttGCGGCCCAGCGGGAAATTGTCCCGATTCCGGCTGTTGTGGAGACGCGGACAATGGCGGTTGCGTGTGAGATTCTGTGAGAAGCGGGAGTCTGGCTGCCCCGGGTTGGCGGCGCGGCGGGGTCAATTGGAGGAGCGATCGTATTCgtac                                                                                                                        >  2:2906565/1‑147 (MQ=255)
                                tGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCa                                                                                                                       <  1:479237/150‑1 (MQ=255)
                                tGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCa                                                                                                                       <  1:1002330/150‑1 (MQ=255)
                                tGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCa                                                                                                                       <  1:1985241/150‑1 (MQ=255)
                                tGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCa                                                                                                                       >  1:1176858/1‑150 (MQ=255)
                                 gCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAg                                                                                                                      >  1:3966344/1‑150 (MQ=255)
                                  cGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAgg                                                                                                                     >  2:4258615/1‑150 (MQ=255)
                                  cGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACCATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGTCAGTGTCAATCGTAGACGCGCTCTTAATCTGCCAgg                                                                                                                     >  2:735126/1‑150 (MQ=255)
                                   ggCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGc                                                                                                                    >  2:2775120/1‑150 (MQ=255)
                                    gCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCt                                                                                                                   <  2:5085983/150‑1 (MQ=255)
                                        aGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCg                                                                                                               >  1:4600564/1‑150 (MQ=255)
                                         gCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCGGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGt                                                                                                              >  2:2262179/1‑150 (MQ=255)
                                             gAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTTGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAg                                                                                                          <  2:182348/150‑1 (MQ=255)
                                              aaaTTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAgg                                                                                                         >  2:5585324/1‑150 (MQ=255)
                                               aaTTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGc                                                                                                        <  2:3140206/150‑1 (MQ=255)
                                               aaTTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGc                                                                                                        >  1:860370/1‑150 (MQ=255)
                                               aaTTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGc                                                                                                        >  2:1515543/1‑150 (MQ=255)
                                                      ccGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATc                                                                                                 <  2:4961238/150‑1 (MQ=255)
                                                        gTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGt                                                                                               >  1:969603/1‑150 (MQ=255)
                                                            ccGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGCTATCGtctct                                                                                           >  2:2675167/1‑150 (MQ=255)
                                                             cGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGtctctc                                                                                          <  1:1401067/150‑1 (MQ=255)
                                                              ggCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTcc                                                                                         >  2:5244767/1‑150 (MQ=255)
                                                               gCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCg                                                                                        <  2:844433/150‑1 (MQ=255)
                                                                cTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCgg                                                                                       >  1:3151890/1‑150 (MQ=255)
                                                                 tgttgtGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGc                                                                                      <  2:5162467/150‑1 (MQ=255)
                                                                    tgtgGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAAt                                                                                   >  1:1609632/1‑150 (MQ=255)
                                                                    tgtgGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAAt                                                                                   <  1:4270731/150‑1 (MQ=255)
                                                                     gtgGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCGGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATg                                                                                  <  2:3312301/150‑1 (MQ=255)
                                                                       ggAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGt                                                                                <  2:2402739/150‑1 (MQ=255)
                                                                             gcTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTAt                                                                          >  2:3480559/1‑150 (MQ=255)
                                                                             gcTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTAt                                                                          >  2:362150/1‑150 (MQ=255)
                                                                             gcTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTAt                                                                          >  2:5447670/1‑150 (MQ=255)
                                                                                  cGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGGCGTACAGGCTGGTTTCGTCTCTCCTGCAATGGAGACTAGCCTgg                                                                     >  2:1665190/1‑150 (MQ=255)
                                                                                        aGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCa                                                               >  1:746268/1‑150 (MQ=255)
                                                                                         ggTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCAc                                                              >  1:266624/1‑150 (MQ=255)
                                                                                          gTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCAcc                                                             >  1:2870947/1‑150 (MQ=255)
                                                                                           ttGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACcg                                                            >  2:1386693/1‑150 (MQ=255)
                                                                                            tGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACcgc                                                           >  1:4069741/1‑150 (MQ=255)
                                                                                               ttttGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGAt                                                        >  2:3590854/1‑150 (MQ=255)
                                                                                               ttttGAGATTCTGAGGGAAACGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGAt                                                        >  2:5842503/1‑150 (MQ=255)
                                                                                                tttGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGAtt                                                       <  1:5291748/150‑1 (MQ=255)
                                                                                                 ttGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGAGTg                                                      >  1:676419/1‑150 (MQ=255)
                                                                                                    agaTTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGGTTGCGa                                                   >  2:1578665/1‑150 (MQ=255)
                                                                                                     gaTTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGTCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAg                                                  >  2:92553/1‑150 (MQ=255)
                                                                                                      aTTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGGGc                                                 >  1:2487441/1‑150 (MQ=255)
                                                                                                         cTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTc                                              <  1:2091473/150‑1 (MQ=255)
                                                                                                         cTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTc                                              <  2:4682846/150‑1 (MQ=255)
                                                                                                         cTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTc                                              >  2:2091473/1‑150 (MQ=255)
                                                                                                            aGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCa                                           >  2:3773729/1‑150 (MQ=255)
                                                                                                             gggAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCaa                                          >  1:1013077/1‑150 (MQ=255)
                                                                                                              ggAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAac                                         <  1:4932390/150‑1 (MQ=255)
                                                                                                               gAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAaca                                        >  2:5702347/1‑150 (MQ=255)
                                                                                                                 aGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCGGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACAcc                                      >  2:5475341/1‑150 (MQ=255)
                                                                                                                    gggAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGc                                   >  1:4439720/1‑150 (MQ=255)
                                                                                                                     ggAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCcgcg                                  >  2:4129154/1‑150 (MQ=255)
                                                                                                                     ggAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCg                                  >  2:3361053/1‑150 (MQ=255)
                                                                                                                         tCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTg                              <  2:1220575/150‑1 (MQ=255)
                                                                                                                          cAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGc                             <  2:2988563/150‑1 (MQ=255)
                                                                                                                          cAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGc                             <  1:1768035/150‑1 (MQ=255)
                                                                                                                             gCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAAt                          <  1:2412585/150‑1 (MQ=255)
                                                                                                                              cTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATg                         <  2:1974592/150‑1 (MQ=255)
                                                                                                                              cTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATg                         <  1:4824759/150‑1 (MQ=255)
                                                                                                                                gCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGc                       <  2:3050847/150‑1 (MQ=255)
                                                                                                                                gCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATAGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGc                       >  2:4491882/1‑150 (MQ=255)
                                                                                                                                gCCCCGGGCTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGc                       <  1:1322029/150‑1 (MQ=255)
                                                                                                                                 ccccGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCg                      >  2:4379095/1‑150 (MQ=255)
                                                                                                                                 ccccGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCg                      <  2:897215/150‑1 (MQ=255)
                                                                                                                                      ggTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTc                 <  1:3383446/150‑1 (MQ=255)
                                                                                                                                        ttGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAg               <  2:1012993/150‑1 (MQ=255)
                                                                                                                                         tGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGc              >  1:5042766/1‑150 (MQ=255)
                                                                                                                                           tcggctcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGccc            <  1:928621/150‑1 (MQ=255)
                                                                                                                                             ggctcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCgg          >  1:2792867/1‑150 (MQ=255)
                                                                                                                                              gctcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGTTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGccccac         >  1:3549449/1‑147 (MQ=255)
                                                                                                                                              gctcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGc         <  1:2580575/150‑1 (MQ=255)
                                                                                                                                               ctcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCt        <  2:104231/150‑1 (MQ=255)
                                                                                                                                                tcggcGGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACCCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCtt       <  1:1830599/150‑1 (MQ=255)
                                                                                                                                                tcggcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCtt       <  2:1609632/150‑1 (MQ=255)
                                                                                                                                                   gcAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCTTAtt    <  1:3288879/150‑1 (MQ=255)
                                                                                                                                                    cAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCTTATTc   >  1:311241/1‑150 (MQ=255)
                                                                                                                                                    cAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCTTATTc   >  1:1101959/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GAAATGATACCGACTCAGCTGTTAGTGGTCATTGCGGCCCAGCGGGAAATTGTCCCGTTTCCGGCTGTTGTGGAGGCGCTGACGATGGAGGTTGCTTTTGAGATTCTGAGGGAAGCGGGAGTCAGGCTGCCCCGGGTTGTCGGCTCGGCAGTGTCAATCGTAGGCGCGCTCGTAATCGGCCAGGCTGCCGTACAGGCTGGTATCGTCTCTCCGGCAATGGTGATTATCGTGGCACTCACCGCGATTGCGAGCTTCGCAACACCGGCGTTTGCAATGGCGATTTCAGCCCGGCTTATTCG  >  CP006881/421128‑421426

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑