BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP006881	3,723,118	C→T	100%	intergenic (‑232/‑69)	U712_19190 ← / → U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006881	3,723,118	0	C	T	99.7%	1345.4 / ‑4.4	380	intergenic (‑232/‑69)	U712_19190/U712_19195	PTS system sucrose‑specific EIIBC component/putative transporter ywcJ
Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (165/214); minor base G (0/1); total (165/215)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 382 total aligned reads displayed.

AATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑G‑TTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGT  >  CP006881/3722972‑3723264

atttttAGGCCTTGCCTCCTTTACCAGTCTCAATCAGCCTTATTCCGATTAAGAATGCGCTTTCATCATAAATTATGATACCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTCTGATATAACATGTAGACAAAAATTCGTAAAAATTa                                                                                                                                                  <  2:1441309/148‑1 (MQ=255)

   tttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAaattaatt                                                                                                                                               >  2:1018036/1‑150 (MQ=255)

    ttAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtg                                                                                                                                              <  2:4564691/150‑1 (MQ=255)

     tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             <  2:3045867/150‑1 (MQ=255)

     tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             <  1:5803886/150‑1 (MQ=255)

     tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             <  1:4495239/150‑1 (MQ=255)

     tAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATtgt                                                                                                                                             >  1:2923723/1‑150 (MQ=255)

       ggTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGa                                                                                                                                           >  2:5006965/1‑150 (MQ=255)

        ggcTTGCCTGCTTTACCAGTCCCAATCCCGCTTATTCAGATTAAGAATACGCTTACATCATAAATCATGATAGCGTTTACGTCATT‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATATCATGTAGACAAAAATTCGTAAAAATTAATTGTGaa                                                                                                                                          <  2:3125369/148‑1 (MQ=255)

          cTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGaaat                                                                                                                                        <  2:1066715/150‑2 (MQ=255)

            tGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATAc                                                                                                                                      <  1:5260825/150‑1 (MQ=255)

               cTGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTc                                                                                                                                   <  2:5442291/150‑1 (MQ=255)

                tGCTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTca                                                                                                                                  >  1:5544851/1‑150 (MQ=255)

                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                                                                                <  2:701638/150‑1 (MQ=255)

                  cTTTACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTcaca                                                                                                                                >  2:3177176/1‑150 (MQ=255)

                   tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                                                                                               <  1:1923492/150‑1 (MQ=255)

                   tttACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACaa                                                                                                                               >  2:2494725/1‑150 (MQ=255)

                     tACCAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAata                                                                                                                             >  2:4774042/1‑150 (MQ=255)

                       ccAGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATc                                                                                                                           <  2:2582477/150‑1 (MQ=255)

                         aGTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGt                                                                                                                         <  2:1495690/150‑1 (MQ=255)

                          gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                                        <  1:1027097/150‑1 (MQ=255)

                          gTCACAATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTg                                                                                                                        <  1:912995/150‑1 (MQ=255)

                              caATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtat                                                                                                                    <  2:535705/150‑1 (MQ=255)

                              caATCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCtat                                                                                                                    >  2:4087094/1‑150 (MQ=255)

                                aTCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATAc                                                                                                                  >  2:2123228/1‑150 (MQ=255)

                                 tCCCGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACt                                                                                                                 <  1:4334686/150‑1 (MQ=255)

                                    cGCTTATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                              <  2:2895389/150‑1 (MQ=255)

                                     gCTTATGCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAACTAT‑T‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAGAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATg                                                                                                              >  1:3798406/1‑150 (MQ=255)

                                      cTTATTCAGACTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTAATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCt                                                                                                            <  1:4017260/150‑1 (MQ=255)

                                       ttATTCATATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTTAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTc                                                                                                           >  1:3687367/1‑150 (MQ=255)

                                       ttATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTc                                                                                                           <  2:3028345/150‑1 (MQ=255)

                                        tATTCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCa                                                                                                          <  2:5500392/150‑1 (MQ=255)

                                          ttCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaat                                                                                                        <  1:3913752/150‑2 (MQ=255)

                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaatc                                                                                                       <  1:4420801/150‑3 (MQ=255)

                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCaatc                                                                                                       >  2:2255841/1‑148 (MQ=255)

                                           tCAGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAAGATCGTGCTATACTATGCTCaatc                                                                                                       >  1:2206568/1‑148 (MQ=255)

                                             aGATTAAGAATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCAt                                                                                                     >  1:1704326/1‑150 (MQ=255)

                                                ttaagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaa                                                                                                  <  2:2687183/147‑1 (MQ=255)

                                                 taagaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaa                                                                                                 >  2:1673326/3‑150 (MQ=255)

                                                    gaATACGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGaaagaa                                                                                              >  1:3104189/1‑150 (MQ=255)

                                                        aCGCTTTCATCATAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCa                                                                                          <  2:2960401/150‑1 (MQ=255)

                                                               catcatAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGaaaag                                                                                   <  1:691468/150‑2 (MQ=255)

                                                                   atAAATCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACaa                                                                               >  1:2354251/1‑150 (MQ=255)

                                                                     aaaTCATGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGGAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAAACATGAAAGAAAGCAGGAAAAGACAATg                                                                             >  2:4471792/1‑150 (MQ=255)

                                                                       atcaTGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa                                                                           <  2:3303906/147‑1 (MQ=255)

                                                                       atcaTGATACCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGa                                                                           >  1:3155433/4‑150 (MQ=255)

                                                                         caTGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGaaa                                                                         >  2:4635365/2‑150 (MQ=255)

                                                                           tGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                       >  2:4181383/1‑150 (MQ=255)

                                                                           tGATAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACt                                                                       <  2:919412/150‑1 (MQ=255)

                                                                             aTAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCa                                                                     <  2:1569625/150‑1 (MQ=255)

                                                                              tAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCaa                                                                    <  1:4110941/150‑1 (MQ=255)

                                                                              tAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCaa                                                                    >  1:3243359/1‑150 (MQ=255)

                                                                              tAGCGTTTTCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCaa                                                                    >  2:4385504/1‑150 (MQ=255)

                                                                                   ttttCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAtt                                                               <  2:1714514/150‑1 (MQ=255)

                                                                                     ttCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCAttac                                                             <  1:3079162/150‑3 (MQ=255)

                                                                                      tCGTCAAC‑‑TAT‑TTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACa                                                            <  1:1183534/150‑1 (MQ=255)

                                                                                        gTCAAC‑‑T‑ATTTTTTTAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACaaa                                                          >  1:2520363/1‑150 (MQ=255)

                                                                                               actatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGt                                                      >  2:5829732/6‑150 (MQ=255)

                                                                                                ctatttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGAAAACTCAAGGATTACAAAAGGtt                                                     >  1:4769832/5‑150 (MQ=255)

                                                                                                   tttttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGaa                                                  <  2:4242681/149‑1 (MQ=255)

                                                                                                     tttttAGTTAAATAGTTTGATATATCATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACa                                                <  2:2717693/150‑1 (MQ=255)

                                                                                                      ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAg                                               <  1:2321120/150‑1 (MQ=255)

                                                                                                      ttttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAg                                               <  2:345891/150‑1 (MQ=255)

                                                                                                       tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGt                                              >  2:1241657/1‑150 (MQ=255)

                                                                                                       tttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGt                                              <  1:3490566/150‑1 (MQ=255)

                                                                                                        ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTGCAAAAGGTTGAACAGTa                                             <  2:1134498/150‑1 (MQ=255)

                                                                                                        ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                             >  2:5230674/1‑150 (MQ=255)

                                                                                                        ttAGTTAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTa                                             >  2:1997313/1‑150 (MQ=255)

                                                                                                            ttAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCt                                         >  1:1318694/1‑150 (MQ=255)

                                                                                                             tAAATAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCtt                                        <  1:3571329/150‑1 (MQ=255)

                                                                                                              aaaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGATCAGTATGCttt                                       <  1:523051/150‑1 (MQ=255)

                                                                                                              aaaTAGTTTGATATAACATGTAGACAAAAATNCGTAAAAAGTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACATAAGGTTGAACAGTATGCttt                                       <  1:2085620/150‑1 (MQ=255)

                                                                                                               aaTAGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTg                                      <  1:2595949/150‑1 (MQ=255)

                                                                                                                  aGTTTGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaa                                   >  1:2730201/1‑149 (MQ=255)

                                                                                                                    tttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAaaaa                                 <  1:55836/150‑1 (MQ=255)

                                                                                                                     ttGATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGaaaaaa                                <  1:1386553/150‑1 (MQ=255)

                                                                                                                       gATATAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAca                              >  1:1492999/1‑149 (MQ=255)

                                                                                                                        atatAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATATTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                             <  1:5147258/150‑3 (MQ=255)

                                                                                                                        atatAACATGTAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAAcaa                             >  2:4513577/1‑148 (MQ=255)

                                                                                                                           taacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAaaaacaaaac                          <  1:3993698/143‑1 (MQ=255)

                                                                                                                            aacatgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAaaacaaaaca                         <  2:3703459/144‑1 (MQ=255)

                                                                                                                              catgtAGACATAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                       <  1:1826385/146‑1 (MQ=255)

                                                                                                                              catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                       <  2:3914445/146‑1 (MQ=255)

                                                                                                                              catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                       >  2:2014437/5‑150 (MQ=255)

                                                                                                                              catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                       >  2:1895162/5‑150 (MQ=255)

                                                                                                                              catgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACata                       <  1:1005277/146‑1 (MQ=255)

                                                                                                                               atgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACatat                      <  2:3155433/147‑1 (MQ=255)

                                                                                                                                tgtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAtt                     >  1:3727120/3‑150 (MQ=255)

                                                                                                                                 gtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAGAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttc                    <  1:4634349/149‑2 (MQ=255)

                                                                                                                                 gtAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttc                    <  2:3673805/149‑2 (MQ=255)

                                                                                                                                  tAGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcg                   <  1:5009543/150‑3 (MQ=255)

                                                                                                                                   aGACAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATAttcgc                  >  2:4932866/1‑147 (MQ=255)

                                                                                                                                      cAAAAATTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTc               <  2:2196182/150‑1 (MQ=255)

                                                                                                                                          aaTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGc           >  2:5622553/1‑150 (MQ=255)

                                                                                                                                           aTTCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGTAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCa          >  1:759518/1‑150 (MQ=255)

                                                                                                                                            ttCGTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCaa         <  1:4963858/150‑1 (MQ=255)

                                                                                                                                               gTAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAAt      <  1:4222641/150‑1 (MQ=255)

                                                                                                                                                tAAAAATTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATc     >  2:5057902/1‑150 (MQ=255)

                                                                                                                                                 aaaaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATcc    >  2:1824636/1‑150 (MQ=255)

                                                                                                                                                   aaaTTAATTGTGAAATACTTCACAATATCGTGCTATACTATGCTCAATCATGAAAGAAAGCAGGAAAAGACAATGGAAACTCAAGCATTACAAAAGGTTGAACAGTATGCTTTGAAAAAACAAAACATATTCGCTTCAAGCAAAATCCGt  >  1:767422/1‑150 (MQ=255)

AATTTTAGGTCTTGCCTGCTTTACCAGTCACAATCCCGCTTATTCAGATTGAGAATACGCTTTCATCATAAATTATGATAGCGTTTTCGTCAAC‑‑T‑G‑TTTTTTAGTTAAATAGTTTGATAAAACTTTTAGACAAAAATTCGTAAAAACTATTTGTGAAAAACTTCACAATATCGTGCTATACTATGCTCAACCATCAAAGAAAGCAGGAAAAAACAATGGAAACTCAAGCATTGCAAAAAGTTGAACAGTACGCTTTGAACAAACTAAACATATTTGTTTCTAGCAAAATCCGT  >  CP006881/3722972‑3723264

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`