Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,041 A→G 6.8% intergenic (+515/+25) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,0410AG 6.8% 411.3 / 7.1 150intergenic (+515/+25)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base A (76/60);  new base G (8/2);  total (87/63)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 157 total aligned reads displayed.

GGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGG  >  NC_000913/1286892‑1287190
                                                                                                                                                     |                                                                                                                                                     
ggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                                                                                                       <  1:634198/150‑1 (MQ=255)
 gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                                                                                                      <  1:3898082/150‑1 (MQ=255)
                cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                                                                                                       <  1:3560636/150‑1 (MQ=255)
                cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTGGGCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGGGAGTTCAGGGACtttt                                                                                                                                       >  2:936771/1‑150 (MQ=255)
                 tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTg                                                                                                                                      <  1:3315439/150‑1 (MQ=255)
                 tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTTGATTGCGGATTCGGTGGTGAGTTCAGGGACTTTTg                                                                                                                                      >  2:2205175/1‑150 (MQ=255)
                    gcgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaaa                                                                                                                                   <  1:3761773/150‑1 (MQ=255)
                      gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                                                                                                 <  1:2310000/150‑1 (MQ=255)
                      gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGt                                                                                                                                 <  2:814477/150‑1 (MQ=255)
                        tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCATCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTGA                                                                                                                               >  2:3588926‑M1/1‑150 (MQ=255)
                            cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGT                                                                                                                           <  2:2610594‑M1/150‑1 (MQ=255)
                               ttCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTGGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGg                                                                                                                        >  2:2849753‑M1/1‑149 (MQ=255)
                                tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTG                                                                                                                       <  1:3547937‑M1/150‑1 (MQ=255)
                                 cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCAAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGG                                                                                                                      <  1:2542416‑M1/150‑1 (MQ=255)
                                  gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGG                                                                                                                     <  2:1381797‑M1/150‑1 (MQ=255)
                                   cccTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCGCGAGGCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAAGGTTTGTTTGCGGGTTGGGTTGAGGGTTGGGGGACTGTTGAAATGGAGGGTGgggggg                                                                                                                    >  2:2472891/1‑150 (MQ=14)
                                      ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAA                                                                                                                 <  2:3548920‑M1/150‑1 (MQ=255)
                                      cgcGGGTCGGGGCCCGCGGCACCGCTCGCTCGCTGGCGCTCTAGCCGCACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCTGAATATTTGCTTGCGGATTCGCTTGAGATTTCTGTGACTTTTGAAAGCGATGGTGGTGGGGGaa                                                                                                                 <  1:1669702/148‑1 (MQ=11)
                                       tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAG                                                                                                                >  2:3080190‑M1/1‑150 (MQ=255)
                                       tCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTATCATCCATACCCCCATGGGCAGAATATTTTATTTCGGATTAGGTTGTTAGTTCAGGGACTTTTTAAAGTGATGGTGGTGGGGGGAG                                                                                                                >  2:702919‑M1/1‑150 (MQ=255)
                                         gggTCGTTGCCTGCGGCAACCCTCTCACGCTGACGCACGAGCCCAACCGTATTCGCAGCGTCTAATACATACCCCCATGGGCAGAATATTTAATTGCGGATTAGGTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGAAggt                                                                                                              >  2:1431515/1‑149 (MQ=2)
                                           gTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                                                                                            <  2:3655247‑M1/150‑1 (MQ=255)
                                           gTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGATCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGATTGAGATTTCATTGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                                                                                            <  1:2680623‑M1/150‑1 (MQ=255)
                                           gTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATT                                                                                                            <  1:3023507‑M1/150‑1 (MQ=255)
                                             cGTTGCGTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCGTCCCCGGAGGGGGAGAATATTTGATTGCGGATTCGCTTGAGTGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTtc                                                                                                          <  1:1317391‑M1/150‑3 (MQ=255)
                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACt                                                                                                         >  1:2554380/1‑150 (MQ=255)
                                              gTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTGGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcga                                                                                                         >  2:2105808‑M1/1‑147 (MQ=255)
                                               ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGACTTTTGAATGTGATGGTGGTGGGGGAAGGATTACTc                                                                                                        >  2:927851/1‑150 (MQ=255)
                                               ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                                                                                                                     >  2:2232105/1‑105 (MQ=255)
                                               ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgaga                                                                                                                                                     <  1:2232105/105‑1 (MQ=255)
                                               ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTagta                                                                                                        >  2:3750017/1‑147 (MQ=255)
                                                  ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                                                                                                     >  2:1796048/1‑150 (MQ=255)
                                                          aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAGTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                                                                             >  2:1814007/1‑150 (MQ=255)
                                                          aaCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCACCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcg                                                                                             >  2:1813925/1‑150 (MQ=255)
                                                           aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTATCAACCTTCCCCCCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTTATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcgc                                                                                            >  1:89105/1‑150 (MQ=255)
                                                             gCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCCTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCtt                                                                                          >  2:2115563/1‑150 (MQ=255)
                                                              ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgcgcttcgcgcttc                                                                                         >  2:413047/1‑150 (MQ=255)
                                                              ctctctCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTc                                                                                         >  2:2104759/1‑150 (MQ=255)
                                                                ctctcGCTGTCGCTCGAGCGGCACCATAGTCGAATACACTCATGCCTACCCTCTTTGGCATAATCTCTGTTTTCGGATTTGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc                                                                                       <  2:3425170/150‑1 (MQ=9)
                                                                    cGCTGGCGCTCCAGTCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATTTTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCtt                                                                                   <  2:2769312/150‑1 (MQ=11)
                                                                       tGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCgg                                                                                <  2:4155006/150‑1 (MQ=255)
                                                                          cGCTCTAGCCGTACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGTGACTTTTGATAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                                                             <  1:1669508/150‑1 (MQ=14)
                                                                          cGCTCGAGCCTAACGTTTGTGGATTCTTCGGATACTTCCACTCTTGGGCAGACTATTTGAGTGCGGATTCGCTTTAGCGTCCTGGGAATTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                                                             <  2:2211423/150‑1 (MQ=9)
                                                                          cGCTCGAGCCGATCCGTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTTGTTAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagattta                                                                             <  1:230845‑M1/150‑32 (MQ=255)
                                                                          cGCTCGAGCCGAACCTTTGTCGAAGCTACTCATCCTTCCCCTCTTGGGCAGAATATTTGATTGCGGTTTCGCTTTAGACTACCGGTAATTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                                                             <  2:176929/150‑1 (MQ=11)
                                                                          cGCCCGAGCCGAACCTTAGTCGACGCGTCTCATCCGTCCCCGCTTGGGCAGAATATTTGAGTGCGGCTCCGCCTGAGCCTTCCTGTACTTTCGCAAGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTc                                                                             <  2:3187756/150‑1 (MQ=11)
                                                                           gCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCTGGTCg                                                                            >  1:1148578/1‑150 (MQ=255)
                                                                           gCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATGTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCATCGGGTCg                                                                            >  2:2064073/1‑150 (MQ=255)
                                                                             tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGtt                                                                          <  2:3430985/150‑1 (MQ=255)
                                                                             tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGtt                                                                          >  1:3690652/1‑150 (MQ=255)
                                                                               gAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGc                                                                        >  2:3147531/1‑150 (MQ=255)
                                                                                 gCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGccg                                                                      >  1:2135617/1‑149 (MQ=255)
                                                                                 gCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAGGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCt                                                                      >  1:3750758/1‑150 (MQ=255)
                                                                                   cGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGc                                                                    >  2:1914808/1‑150 (MQ=255)
                                                                                     aaCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGcag                                                                  >  2:1671648/1‑148 (MQ=255)
                                                                                            gTCGAAGCTTCTGCTCCTTCCCCGATGGGGCATATGAATTGATTGGGGATTCGCTAGAGCTTTCAGGGACTTTTGAAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGct                                                           <  2:3086052/150‑1 (MQ=11)
                                                                                            gTCGAAGCTTCTCATCCTTCCCGGCTTGGGATTAATTTTTGTTTGCGTATTCGCTTTAGTGTTCTGGTACTTTTGAATGTTTTGGTGGTGGGGGATGGTTTACTCAGCGCTGCGGGCTTCGCCCTTGGGGTGGTTGCCGCTGGCAACgac                                                           <  2:89105/150‑3 (MQ=2)
                                                                                            gTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGca                                                           >  1:1900710/1‑149 (MQ=255)
                                                                                                aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGctctct                                                       >  1:2605230/1‑150 (MQ=255)
                                                                                                aaGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTGGCCTGCGGCAGCGctctct                                                       >  1:2605372/1‑150 (MQ=255)
                                                                                                   cTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAACCGCTCTCGc                                                    >  2:3251748/1‑150 (MQ=255)
                                                                                                   cTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTGCCTGCGGCAACGCtcgctcgc                                                    >  2:3600935/1‑150 (MQ=255)
                                                                                                    ttCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGTTGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt                                                   <  1:58803/150‑1 (MQ=255)
                                                                                                    ttCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCt                                                   <  1:2411376/150‑1 (MQ=37)
                                                                                                     tctcATCCCTCCCCGCAAGTGCATAATATATGCTGGCTGTTTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTg                                                  <  2:32073/150‑1 (MQ=14)
                                                                                                        cATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGgcg                                               <  2:4085772/150‑1 (MQ=255)
                                                                                                             ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCTCCCTTCGGGTCGTTTCATGCGGCAACGCTATCTCGCTGGCGATCGa                                          >  2:2540443/1‑150 (MQ=14)
                                                                                                             ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCCGCAACGCTCTCTCGCTGGCGCACGa                                          >  2:1506173/1‑150 (MQ=14)
                                                                                                              tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTCCCGCAACGCACTCTCGCAGgcgcacccg                                         >  2:735737/1‑145 (MQ=14)
                                                                                                              tCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACACAGCTCGGAGACCTTCGCCCATCTGGTCGTTGCAGCCCGCAACGCTCTCACACAGGCGCTCCAg                                         >  2:4154572/1‑150 (MQ=14)
                                                                                                               ccccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGt                                        <  2:668588/150‑1 (MQ=255)
                                                                                                                cccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTTCGCGCATCTCCCTTCTGGTCGTTGCATGCGGCAACGCACTCTCGCTGACGCTCGAGTc                                       >  2:3097034/1‑150 (MQ=14)
                                                                                                                       gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCCGGCGCACGACACGAACCtt                                >  1:3357323/1‑150 (MQ=14)
                                                                                                                        ggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTTAAAGTGATGGTGTTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                               <  1:1157548/150‑1 (MQ=255)
                                                                                                                         gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                              >  1:1013877/1‑150 (MQ=255)
                                                                                                                         gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGAGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                              >  2:2737999/1‑150 (MQ=255)
                                                                                                                          cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                             <  1:1914808/150‑1 (MQ=255)
                                                                                                                          cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                             >  2:1482043/1‑150 (MQ=255)
                                                                                                                           aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                            >  1:4050104/1‑150 (MQ=255)
                                                                                                                             aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                          >  1:3705183/1‑150 (MQ=255)
                                                                                                                             aaTATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATGGTCGa                          >  2:1550959/1‑150 (MQ=255)
                                                                                                                              atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                         <  1:860646/150‑1 (MQ=255)
                                                                                                                              atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                         <  1:1796048/150‑1 (MQ=255)
                                                                                                                                 tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCt                      >  2:542948/1‑150 (MQ=255)
                                                                                                                                 tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGAGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCt                      >  2:2224575/1‑150 (MQ=255)
                                                                                                                                   taattGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTcgaaccttcgaagtcgatgacggcagatttacagtctgctccctttggccgctcgggaaccccaccacggggtaatgctttttactgg                    <  2:1995642‑M1/148‑89 (MQ=255)
                                                                                                                                      ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCATTGTCGAAGCTTCTCa                 >  2:2613071/1‑150 (MQ=255)
                                                                                                                                      ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCGCGAGTCGAACCGTGGTCGAAGCTTCTCa                 >  1:4046415/1‑150 (MQ=255)
                                                                                                                                      ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCCGCAACGCTATAACGCGGGCGCGCGAGTCGAACCGTTGGCGAAACTTCTCa                 >  1:101250/1‑150 (MQ=14)
                                                                                                                                      ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCAtcgaa                 >  2:3507763/1‑147 (MQ=255)
                                                                                                                                      ttGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCGTCGCCCTTCGGGTCGTTGCCAGCGGCCACCCTCTCTCGCTGGCGCTCGAGTAGAACCTTGGTCGTAGCTTCTCa                 >  1:4046545/1‑150 (MQ=14)
                                                                                                                                        gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc               >  2:2144739/1‑150 (MQ=255)
                                                                                                                                        gCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGAGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc               >  2:2144909/1‑150 (MQ=255)
                                                                                                                                        gCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATc               <  2:1650660/150‑1 (MQ=255)
                                                                                                                                           gATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGACGCTTCTCATCCtt            >  2:2380090/1‑150 (MQ=255)
                                                                                                                                              tCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCACTCGCGGGCGCACCAGTCGAACCATGGTAGAAGCTTATCAAccatacc         >  1:3950526/1‑145 (MQ=14)
                                                                                                                                                 cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGc      >  2:3761763/1‑150 (MQ=255)
                                                                                                                                                 cTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCAGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCga      >  1:3919707/1‑149 (MQ=255)
                                                                                                                                                  ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCa     <  1:1437241/150‑1 (MQ=255)
                                                                                                                                                  ttGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGAGGCCACCCTCACACGCTGGCGCACGAATCGAACCTTGGTCGAAGCTTCTCATCCTTACCCCCa     >  1:357066/1‑150 (MQ=14)
                                                                                                                                                     |                                                                                                                                                     
GGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGG  >  NC_000913/1286892‑1287190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: