Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,552,270 T→G 6.0% intergenic (+279/+128) fdnI → / ← yddM formate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,552,2700TG 6.0% 708.0 / 30.1 219intergenic (+279/+128)fdnI/yddMformate dehydrogenase‑N, cytochrome B556 (gamma) subunit, nitrate‑inducible/putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (115/89);  new base G (11/2);  total (128/91)
Fisher's exact test for biased strand distribution p-value = 7.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.03e-01

Only 100 of 220 total aligned reads displayed.

ATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCG  >  NC_000913/1552121‑1552418
                                                                                                                                                     |                                                                                                                                                    
aTAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCt                                                                                                                                                      >  2:137883/1‑150 (MQ=255)
  aGATTGCAGTGAACGTGTAGGCCTGATAATCGTAGCGCATCAGGCAATGTTGAGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTa                                                                                                                                                    <  1:2231548/150‑1 (MQ=255)
     ttGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAAAAATGGCGCTGTAAAAGGCGTCATTTTAATATTGTAGACAACGTGGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATcg                                                                                                                                                 >  2:1437631/1‑149 (MQ=255)
       gCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                               <  2:3318810/150‑1 (MQ=255)
       gCAGTGAACGTGTAGGCCTGATAAGCGTAGCGAATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCgg                                                                                                                                               <  2:3433904/150‑1 (MQ=255)
         aGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATc                                                                                                                                                  <  1:530835/145‑1 (MQ=255)
         aGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCa                                                                                                                                             <  2:1805507/150‑1 (MQ=255)
          gTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTACAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCAt                                                                                                                                            >  2:1769102/1‑150 (MQ=255)
           tGAACGTTTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                                                                                                           <  1:2990341/150‑1 (MQ=255)
           tGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTTCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                                                                                                           <  2:2030817/150‑1 (MQ=255)
           tGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATg                                                                                                                                           <  1:3247713/150‑1 (MQ=255)
             aaCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGaa                                                                                                                                         <  2:3976981/150‑1 (MQ=255)
                    cggCCTGAGAAGCGGAGCGCCGCAGGCAATGTTGCGTTTGTCCTCAGGCTCAACTGGCGCAGTAAACGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGACCGCCTTATCCGGCATGAAAACCCtt                                                                                                                                  <  2:654830/149‑1 (MQ=17)
                    aGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCtt                                                                                                                                  <  2:3738249/150‑1 (MQ=255)
                     ggCCTGATACGCGTAGCGCATCAGTCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTc                                                                                                                                 <  2:2076484/150‑1 (MQ=255)
                      gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                                                                                                >  1:87018/1‑150 (MQ=255)
                      gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                                                                                                >  1:1903928/1‑150 (MQ=255)
                      gCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCa                                                                                                                                >  1:2250528/1‑150 (MQ=255)
                         tGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAAt                                                                                                                             <  1:137883/150‑1 (MQ=255)
                           aTAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAAGCGGCGTGAAAGACTGATCCGGCCTGAAAACCCTTCAAATcc                                                                                                                           >  2:1195510/1‑150 (MQ=21)
                              aGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                                                                                                        >  2:3112873/1‑150 (MQ=255)
                              aGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAt                                                                                                                        <  2:1283921/150‑1 (MQ=255)
                                    gcgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGTTGCGGCGTGTAGGGGTTATGCGGCTTGAAAACCCTTCAAATCCAATAGATTg                                                                                                                  >  2:1634951/1‑150 (MQ=18)
                                     cgcATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGc                                                                                                                 >  1:401774/1‑150 (MQ=255)
                                       cATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAg                                                                                                               >  2:2772030/1‑150 (MQ=255)
                                        aTCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGt                                                                                                              >  2:858929/1‑150 (MQ=255)
                                        aTCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGt                                                                                                              >  2:1902743/1‑150 (MQ=255)
                                            ggCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGAGGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAAc                                                                                                          >  1:453205/1‑150 (MQ=255)
                                             gCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACg                                                                                                         >  2:2935487/1‑150 (MQ=255)
                                              cAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTTTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgt                                                                                                        <  1:3348091/150‑1 (MQ=255)
                                               aaTGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATAAAATAGATTGCAGTGAACgtg                                                                                                       >  1:3310083/1‑150 (MQ=255)
                                                aTGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGAAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                                                                                      <  1:969992/150‑1 (MQ=255)
                                                   ttGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAgg                                                                                                   <  2:2976432/150‑1 (MQ=255)
                                                    tGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGc                                                                                                  <  1:4029429/150‑1 (MQ=255)
                                                      cGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCt                                                                                                <  1:933371/150‑1 (MQ=255)
                                                            tcatcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAg                                                                                          >  2:3637209/1‑150 (MQ=40)
                                                             catcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGc                                                                                         <  2:292154/150‑1 (MQ=40)
                                                               tcaGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGt                                                                                       >  2:1512915/1‑150 (MQ=38)
                                                                 aGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAg                                                                                     >  1:2132204/1‑150 (MQ=37)
                                                                   tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcg                                                                                   >  1:3674458/1‑150 (MQ=35)
                                                                   tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcg                                                                                   >  1:3645845/1‑150 (MQ=35)
                                                                   tttCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAgcg                                                                                   >  1:788822/1‑150 (MQ=35)
                                                                    ttaaaaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACgtgt                                                                                                      <  1:3364243/127‑1 (MQ=14)
                                                                     tCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCa                                                                                 >  1:2272362/1‑150 (MQ=33)
                                                                      cAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAt                                                                                <  2:1931314/150‑1 (MQ=32)
                                                                      aaaaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATa                                                                                                                       <  1:2597327/110‑1 (MQ=14)
                                                                       aaaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATCGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATc                                                                               >  1:1181840/1‑150 (MQ=17)
                                                                        aaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCa                                                                              >  1:2626122/1‑150 (MQ=32)
                                                                        aaTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCa                                                                              >  1:2626554/1‑150 (MQ=32)
                                                                         aTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATCGATTGCAGTGCACGTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                             >  2:1353837/1‑150 (MQ=17)
                                                                         aTGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGACTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGTAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAg                                                                             >  1:1454512/1‑150 (MQ=17)
                                                                              gcTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                        >  2:2730894/1‑150 (MQ=32)
                                                                              gcTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                        >  2:112944/1‑150 (MQ=32)
                                                                              gcTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAAt                                                                        >  1:2097604/1‑150 (MQ=32)
                                                                                tGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGt                                                                      >  2:3089132/1‑150 (MQ=32)
                                                                                   aaaaGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGc                                                                   <  1:390380/150‑1 (MQ=32)
                                                                                    aaaGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCg                                                                  >  2:69114/1‑150 (MQ=32)
                                                                                     aaGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGt                                                                 <  1:1790148/150‑1 (MQ=32)
                                                                                      aGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGtt                                                                <  1:1734031/150‑1 (MQ=32)
                                                                                         cGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGt                                                             <  2:2699389/150‑1 (MQ=32)
                                                                                                      ttGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTaaa                                                >  2:2652195/1‑150 (MQ=33)
                                                                                                      ttGTAGACAACGTAGGCTTGGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTGTGTCATCAGTTCTaaa                                                >  2:3805759/1‑150 (MQ=18)
                                                                                                            aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGgcgc                                          >  2:678053/1‑150 (MQ=34)
                                                                                                            aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGgcgc                                          >  2:1086020/1‑150 (MQ=34)
                                                                                                            aCAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAAGAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGgcgc                                          >  2:2390087/1‑150 (MQ=21)
                                                                                                             cAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCt                                         <  1:292600/150‑1 (MQ=34)
                                                                                                                   aGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATaa                                   <  2:4052985/150‑1 (MQ=39)
                                                                                                                     gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAg                                 <  2:1755996/150‑1 (MQ=40)
                                                                                                                     gCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAg                                 <  1:3112873/150‑1 (MQ=40)
                                                                                                                          gTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATGGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTGGTCAGCAGTTCTAAATGGCGCTTTATAAAGTGCCa                            >  2:2562676/1‑150 (MQ=255)
                                                                                                                             cATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAttt                         <  1:2073804/150‑1 (MQ=255)
                                                                                                                             cATGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCACTGTTGCGTTAGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAttt                         >  2:2904223/1‑150 (MQ=14)
                                                                                                                               tGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTTTAAAGTGCCAttttt                       >  1:2755123/1‑150 (MQ=255)
                                                                                                                               tGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTAGAAATGGCGCTGTATAAAGAGACAttttg                       >  2:2479054/1‑149 (MQ=11)
                                                                                                                               tGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAAACCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCAGCAGGCAATGTTGCGGTTGTCATCAGTTCTAAATGGCGCGTTATAAAGTGCCAttttt                       >  1:3284007/1‑150 (MQ=255)
                                                                                                                               tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGACCGTGTAGGCCTGATAAGCGTAGCGCCTCAGGCAATGTTGCGGGTGTCAGCAGTTCTAAATGGCGCTGTATAAAGTGCCAttttt                       >  2:118603/1‑150 (MQ=14)
                                                                                                                               tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAttttt                       >  1:421482/1‑150 (MQ=14)
                                                                                                                               tGCCGGATAAGGCGTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGGGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAttttt                       >  2:1133629/1‑150 (MQ=14)
                                                                                                                                 ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAttttttt                     >  2:746895/1‑150 (MQ=255)
                                                                                                                                 ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCACTTTATAAAGCGCCAtt                          <  1:2325164/145‑1 (MQ=255)
                                                                                                                                 ccGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCACTTTATAAAGCGCCAtt                          >  2:2325164/1‑145 (MQ=255)
                                                                                                                                    gataaggcgTTCACGCCGCATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTAtt                  >  1:2318624/6‑150 (MQ=17)
                                                                                                                                    gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTAtt                  <  2:2250554/150‑1 (MQ=255)
                                                                                                                                    gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTAtt                  <  1:1623604/150‑1 (MQ=255)
                                                                                                                                    gATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTAtt                  <  1:1623417/150‑1 (MQ=255)
                                                                                                                                       gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCg               >  1:2857193/1‑150 (MQ=255)
                                                                                                                                       gcggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCg               <  2:4106097/150‑1 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTa             >  1:471464/1‑150 (MQ=255)
                                                                                                                                         ggcgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTa             >  1:2752223/1‑150 (MQ=255)
                                                                                                                                           cgTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAAc           <  1:1336975/150‑1 (MQ=255)
                                                                                                                                             tGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCa         <  2:122685/150‑1 (MQ=255)
                                                                                                                                               aaCGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGa       >  1:1516225/1‑150 (MQ=255)
                                                                                                                                                 cGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACg     >  2:54872/1‑150 (MQ=255)
                                                                                                                                                 cGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTGTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACg     >  2:2162499/1‑150 (MQ=255)
                                                                                                                                                  gCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACgg    >  1:3479450/1‑150 (MQ=255)
                                                                                                                                                  gCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACgg    >  1:2459553/1‑150 (MQ=255)
                                                                                                                                                   ccTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGc   <  2:453205/150‑1 (MQ=255)
                                                                                                                                                   ccTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCACCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCAATTTTTTATTGCGTAACCAGACGGc   <  1:2491343/150‑1 (MQ=255)
                                                                                                                                                    cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCg  >  1:1886503/1‑150 (MQ=255)
                                                                                                                                                    cTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCg  >  1:1011718/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
ATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTTCAAATGGCGCTGTAAAAGGCGTCATTTTCATATTGTAGACAACGTAGGCTTTGTTCATGCCGGATGCGGCGTGAACGCCTTATCCGGCATGAAAACCCTTCAAATCCAATAGATTGCAGTGAACGTGTAGGCCTGATAAGCGTAGCGCATCAGGCAATGTTGCGTTTGTCATCAGTTCTAAATGGCGCTTTATAAAGTGCCATTTTTTTATTGCGTAACCAGACGGCG  >  NC_000913/1552121‑1552418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: