Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,635,942 T→A 5.5% pseudogene (426/528 nt) nohQ ← pseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,635,9420TA 5.5% 267.4 / 3.0 83pseudogene (426/528 nt)nohQpseudogene, Qin prophage; Phage DNA packaging protein Nu1 family;Phage or Prophage Related; DNA packaging protein NU1 homolog from lambdoid prophage Qin
Reads supporting (aligned to +/- strand):  ref base T (55/23);  new base A (2/3);  total (57/26)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCT  >  NC_000913/1635794‑1636090
                                                                                                                                                    |                                                                                                                                                    
taACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGtt                                                                                                                                                     <  2:281982/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:1428266/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:1238729/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:579120/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  1:1682343/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  1:728002/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:3091386/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:1195681/150‑1 (MQ=255)
 aaCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTc                                                                                                                                                    <  2:3091453/150‑1 (MQ=255)
  aCTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTcc                                                                                                                                                   <  2:1140009/150‑1 (MQ=255)
    tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg                                                                                                                                                 >  1:759233/1‑150 (MQ=255)
    tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg                                                                                                                                                 >  2:1841595/1‑150 (MQ=255)
    tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg                                                                                                                                                 >  1:2117455/1‑150 (MQ=255)
    tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg                                                                                                                                                 >  2:566562/1‑150 (MQ=255)
    tCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGAATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCgg                                                                                                                                                 >  2:2733534/1‑150 (MQ=255)
    acAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCACGTTTCAGGAAATCAACATGTAGGTTTTCCAGTTcctg                                                                                                                                                 >  1:1245830/2‑148 (MQ=255)
     cAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGa                                                                                                                                                >  1:248147/1‑150 (MQ=255)
     cAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGa                                                                                                                                                >  1:4054416/1‑150 (MQ=255)
      aaGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGACATCAACATGTCGGTTTTCCAGTTCCGGaa                                                                                                                                               >  2:2140312/1‑150 (MQ=255)
      aaGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGaa                                                                                                                                               >  2:427297/1‑150 (MQ=255)
      aaGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGaa                                                                                                                                               >  2:1873811/1‑150 (MQ=255)
      aaGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGaa                                                                                                                                               >  2:1296810/1‑150 (MQ=255)
      aaGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACAAGTAGGTTTTCAAGTTCCGGaa                                                                                                                                               >  1:2198940/1‑150 (MQ=255)
         aCCAGCTGCCACCTGCGTATAGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCAACAAGCGCGGCTGCTTTGTTCATGGCGTTGATGATATCCCGATTCAGTAAATCAACATGTCGGTTTTCCAGAAACGGAAAAc                                                                                                                                            >  2:1680393/1‑150 (MQ=17)
           ctgctgCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAAcgc                                                                                                                                          <  1:3112554/150‑1 (MQ=255)
            tgctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAAcgcc                                                                                                                                         >  1:790934/1‑150 (MQ=255)
             gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCGGAACTGGAAAAccgac                                                                                                                                        <  2:886469/150‑5 (MQ=17)
             gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCGGAACTGGAAAAccgac                                                                                                                                        <  2:1438175/150‑5 (MQ=17)
             gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAAcgccg                                                                                                                                        >  2:2330639/1‑150 (MQ=255)
             gctgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGATTTCCGGAACTGGAAAAccgac                                                                                                                                        <  2:1010249/150‑5 (MQ=17)
               tgcCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCt                                                                                                                                      >  1:1786631/1‑150 (MQ=255)
                  ccccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCa                                                                                                                                   >  1:2327084/1‑150 (MQ=255)
                  ccccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCa                                                                                                                                   >  1:2327016/1‑150 (MQ=255)
                   cccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAc                                                                                                                                  >  2:1402056/1‑150 (MQ=255)
                   cccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAc                                                                                                                                  >  2:4071489/1‑150 (MQ=255)
                   cccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAc                                                                                                                                  >  2:955869/1‑150 (MQ=255)
                   cccTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCAc                                                                                                                                  >  1:521002/1‑150 (MQ=255)
                     cTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCg                                                                                                                                >  1:618304/1‑150 (MQ=255)
                     cTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCg                                                                                                                                >  2:4074865/1‑150 (MQ=255)
                     cTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCg                                                                                                                                >  2:4074976/1‑150 (MQ=255)
                     cTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCg                                                                                                                                >  2:3444359/1‑150 (MQ=255)
                          tATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAgg                                                                                                                           >  1:199967/1‑150 (MQ=255)
                          tATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAgg                                                                                                                           >  1:3395711/1‑150 (MQ=255)
                           tttGTCTTTCCGCGCGGTTATATTCACTCAGCAACCCGGTTATCAGTTCATCCTGCGGGGCTGCTTTGTTCTTGGCTTTGTTGATACCCCTTTACAGTAAATCAACTTGTGGTTTTTCCTGTTCCGCAAAACGCCGCTGCACCGACAggg                                                                                                                          <  1:3128189/149‑1 (MQ=11)
                           aTTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAggg                                                                                                                          <  2:405118/150‑1 (MQ=255)
                           aTTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAggg                                                                                                                          <  2:2433697/150‑1 (MQ=255)
                             tgtctgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAggggg                                                                                                                        >  1:1208630/1‑150 (MQ=255)
                               tctgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATAGCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGAt                                                                                                                      <  1:2525686/150‑1 (MQ=255)
                                ctgtcCGCGCGGTTATTTTCACTCAGAAACCCCGTTATCATTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATc                                                                                                                     <  1:284575/150‑1 (MQ=11)
                                ctgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATc                                                                                                                     >  1:2703755/1‑150 (MQ=40)
                                ctgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATc                                                                                                                     <  2:176026/150‑1 (MQ=40)
                                 tgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGGTcc                                                                                                                    >  1:2970455/1‑150 (MQ=255)
                                 tgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATcc                                                                                                                    >  1:3587476/1‑150 (MQ=39)
                                 tgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATcc                                                                                                                    >  1:2049507/1‑150 (MQ=39)
                                 tgtcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATcc                                                                                                                    >  1:1673076/1‑150 (MQ=39)
                                 tgtcAGCGCGGTTATAGTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGCTcc                                                                                                                    >  2:3644920/1‑150 (MQ=255)
                                   tcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCg                                                                                                                  >  1:2953592/1‑150 (MQ=38)
                                   tcAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCg                                                                                                                  >  1:2953868/1‑150 (MQ=38)
                                   tcAGCGCGGTTAAATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTGTGTTCAAGGCTTTGAAGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGAGCCGGACAACGCCGCTCCACCGCCAGGGGGATCCCg                                                                                                                  >  2:2755797/1‑150 (MQ=11)
                                     aGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTc                                                                                                                >  1:1187370/1‑150 (MQ=35)
                                     aGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTc                                                                                                                >  1:568099/1‑150 (MQ=35)
                                      gcgcGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCg                                                                                                               >  2:2658257/1‑150 (MQ=35)
                                       cgcgGTTATATTCACTCCGCAACCCCGGGATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCga                                                                                                              <  2:2013507/150‑1 (MQ=21)
                                       cgcgGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACTTGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCga                                                                                                              <  1:3751227/150‑1 (MQ=21)
                                       cgcgGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCga                                                                                                              >  2:4095540/1‑150 (MQ=34)
                                       cgcgGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCga                                                                                                              >  2:4095508/1‑150 (MQ=34)
                                       cgcgGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCga                                                                                                              <  2:2013506/150‑1 (MQ=34)
                                          ggTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGaa                                                                                                           >  2:317454/1‑150 (MQ=32)
                                          ggTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGaa                                                                                                           >  2:137376/1‑150 (MQ=32)
                                           gTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAAt                                                                                                          >  2:2846511/1‑150 (MQ=31)
                                           gTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGAAATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGCGGATCCCGTCGAGAAt                                                                                                          >  2:275587/1‑150 (MQ=14)
                                            ttATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATa                                                                                                         >  1:2461748/1‑150 (MQ=11)
                                                                        gtacATCCCGGGCGGCTGCTTTGTTCATGGCTTTGTTGATATCCCGTTTCAGTAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACa                                                                             <  2:3508616/147‑1 (MQ=1)
                                                                                                                                            ttttCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCg         >  1:584835/1‑150 (MQ=11)
                                                                                                                                               tCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCg      >  1:2180117/1‑150 (MQ=32)
                                                                                                                                                ccAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCgg     >  1:3759684/1‑150 (MQ=32)
                                                                                                                                                 cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGc    >  2:3573338/1‑150 (MQ=32)
                                                                                                                                                 cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGc    <  1:2658257/150‑1 (MQ=32)
                                                                                                                                                 cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGc    <  1:1841595/150‑1 (MQ=32)
                                                                                                                                                 cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGc    >  2:574462/1‑150 (MQ=32)
                                                                                                                                                 cAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGc    <  2:759233/150‑1 (MQ=32)
                                                                                                                                                  aGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGcc   >  2:1385916/1‑150 (MQ=32)
                                                                                                                                                  aGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGcc   >  2:789725/1‑150 (MQ=32)
                                                                                                                                                   gTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCt  >  2:1036635/1‑150 (MQ=32)
                                                                                                                                                    |                                                                                                                                                    
TAACTCAAGACCTGCTGCCCCCTGCGTATTGTCTGTCAGCGCGGTTATATTCACTCAGCAACCCCGGTATCAGTTCATCCAGCGCGGCTGCTTTGTTCATGGCTTTGATGATATCCCGTTTCAGGAAATCAACATGTCGGTTTTCCAGTTCCGGAAAACGCCGCTGCACCGACAGGGGGATCCCGTCGAGAATACTGGCAATTTCACCTGCGATCCGCGACAGCACGAAAGTACAGAATGCGGTTTCCACCACTTCAGCGGAGTCTCTGGCATTTTTCAGCTCCTGTGCGTCGGCCT  >  NC_000913/1635794‑1636090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: