BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	1,856,333	T→G	5.9%	E199A (GAG→GCG) ‡	ydjH ←	putative kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,333	0	T	G	5.9%	634.1 / 23.1	191	E199A (GAG→GCG) ‡	ydjH	putative kinase
Reads supporting (aligned to +/- strand): ref base T (103/75); new base G (7/5); total (111/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 195 total aligned reads displayed.

CTGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCA  >  NC_000913/1856184‑1856482

 tGCGACCGCCGGCACCTTCATCGTCAGGTCACCCCGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTc                                                                                                                                                      <  2:1400325/150‑1 (MQ=255)

   cGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCgg                                                                                                                                                    <  1:2036777/150‑1 (MQ=255)

     aCCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCa                                                                                                                                                  >  2:1771709/1‑150 (MQ=255)

       cgccgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCaaa                                                                                                                                                <  2:579973/150‑1 (MQ=255)

         ccgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAAt                                                                                                                                              <  1:30063/150‑1 (MQ=255)

          cgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                                                                             >  2:1162848/1‑150 (MQ=255)

          cgGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAAtt                                                                                                                                             >  2:2926488/1‑150 (MQ=255)

           ggCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGAGGCAAAATTa                                                                                                                                            <  2:540378/150‑1 (MQ=255)

             cACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                                                                          <  2:1837613/150‑1 (MQ=255)

             cACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAgg                                                                                                                                          <  2:3310061/150‑1 (MQ=255)

                cgacagagTCAAGTCACCACGCAGGATAAAGCAGCCGTCTTTACCCGTCTTAATCACCCCCGTTGTAACGCCGCACCCAAGAAAGCAGTCACCAATTTCATCCAGTGTCTCATTCCCGGTGAGAAAATTTGCCACGGCAAAATTAGGaaa                                                                                                                                       >  2:2492595/8‑150 (MQ=255)

                 ttCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                                                                      >  2:1111876/1‑150 (MQ=255)

                 ttCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAc                                                                                                                                      >  2:261118/1‑150 (MQ=255)

                  tCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTAACCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTATTCCTCGGCAAAATTAGGAAACa                                                                                                                                     >  2:246379/1‑150 (MQ=255)

                     tCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGAt                                                                                                                                  <  2:702152/150‑1 (MQ=255)

                          aTGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                                                                             <  2:2827749/150‑1 (MQ=255)

                          aTGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCg                                                                                                                             <  2:2827727/150‑1 (MQ=255)

                           gggcaccacGCTGGAGACAGCAGCCGTCTTTACCCGTTTGAAGCACCACCGTTGTTACGCCGCCCGCAAGAACGCAGTCAGCAATTACATCCAGTGTCTCTGTCCCGGGGAGTAATTTTGCCGCGGCAAAATTAGGACACAGATAATCGa                                                                                                                            <  2:2165050/147‑1 (MQ=255)

                                 cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAATATTAGGAAACAGATAATCGACATAAc                                                                                                                      >  2:2322099/1‑150 (MQ=255)

                                 cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                                                                      >  1:2847145/1‑150 (MQ=255)

                                 cacGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAAc                                                                                                                      >  1:3728836/1‑150 (MQ=255)

                                   cGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCCAAATTAGGAAACAGATAATCGACATAACTc                                                                                                                    >  1:848217/1‑150 (MQ=255)

                                       tGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATg                                                                                                                <  2:462142/150‑1 (MQ=255)

                                        gATAAAGCAGCCGTCTTAACCCGTTTTAATCACCACCGTATTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCCTCCAGTGTCTCTTTCCCGGAGAGTAATTTTGCCTCGGCCAAATTAGGAAACAGATAATCGACATAACTCAATGc                                                                                                               >  2:194367/1‑150 (MQ=255)

                                                 gcCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTATTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAat                                                                                                      <  1:2348006/150‑1 (MQ=255)

                                                    gTCTTTACCCGTTTTAATAACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATATCAACCAGTGTATCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAAAAGATAAACGACATAAATCAATGCTTAGCAAATatc                                                                                                   >  2:1637061/1‑150 (MQ=255)

                                                    gTCTTTACCCGTATTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatc                                                                                                   <  2:4017664/150‑1 (MQ=255)

                                                     tCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATatca                                                                                                  >  2:1061036/1‑150 (MQ=255)

                                                          aCCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATAGACATAACTCAATGCTTCGCAAATATCATCCAg                                                                                             >  1:1870425/1‑150 (MQ=255)

                                                           cccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAAAATCATACAGc                                                                                            >  1:2905835/1‑150 (MQ=255)

                                                            ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATTCTTCGCAAATATAATCCAGCg                                                                                           >  1:3083656/1‑150 (MQ=255)

                                                            ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCg                                                                                           >  2:2058570/1‑150 (MQ=255)

                                                            ccGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCGCGGCAAAATTAGGAAACAGATAATCGACATAAATCAATGCTTCGCAAATATCATCCAGCg                                                                                           >  1:1184502/1‑150 (MQ=255)

                                                            ccGTTTTAATCACCACCGTTTTAAAGCCGCAAGCAAGAAAGCAGTCAGCAATTACATCCATTGACTCATTCCCGGTGAGTAATTTTGACTCGGAAAAAATAGGAAAAATAAAATAGACAAAACTAAAAGCTTAGCAAATATCATCCAGct                                                                                           >  1:335465/1‑149 (MQ=255)

                                                               ttttAATCACCACCGTTTTTACGCCGCCCGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGttt                                                                                        <  2:1883035/150‑1 (MQ=255)

                                                                 ttAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttca                                                                                      <  1:2515888/150‑1 (MQ=255)

                                                                    aTCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTttcattc                                                                                   <  2:2000687/150‑1 (MQ=255)

                                                                       accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                                                                                >  1:2004580/1‑150 (MQ=255)

                                                                       accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                                                                                >  1:3855953/1‑150 (MQ=255)

                                                                       accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAAc                                                                                >  1:829270/1‑150 (MQ=255)

                                                                       accaccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTAATCCAGTGTCTCTTTCCCTGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACACAATGCTTCGCAAATATCATCCAGCGATTCAGTCAAc                                                                                >  1:2728884/1‑150 (MQ=255)

                                                                         caccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcg                                                                              >  2:2374871/1‑150 (MQ=255)

                                                                          accGTTTTTACGCCGCACGCAAGAAAGCAGACAGCAATTTCATCCAGTGTCTCTTTCCCGGGGAGTAATTTTGCCTCGGCACAATTAGGAAACAGATAATCGACATAACTCAATGCATCGCAAAAATCATCCAGCGGTGCAATCAACcgc                                                                             >  2:3363752/1‑150 (MQ=255)

                                                                           ccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCGATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAata                                                                                                     >  1:2425280/1‑125 (MQ=255)

                                                                           ccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                                                                            >  1:673400/1‑150 (MQ=255)

                                                                           ccGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACcgcg                                                                            >  1:519831/1‑150 (MQ=255)

                                                                                  tACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGAt                                                                     <  2:964443/150‑1 (MQ=255)

                                                                                    cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                                                                   <  2:149076/150‑1 (MQ=255)

                                                                                    cgccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCa                                                                   <  2:153729/150‑1 (MQ=255)

                                                                                     gccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCat                                                                  >  1:3464639/1‑150 (MQ=255)

                                                                                      ccgcACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCata                                                                 >  1:4136570/1‑150 (MQ=255)

                                                                                       cgcacgcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCatat                                                                <  1:1540001/150‑1 (MQ=255)

                                                                                            gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGca                                                           <  2:2723001/150‑1 (MQ=255)

                                                                                            gcaAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGca                                                           <  1:1010289/150‑1 (MQ=255)

                                                                                                 aaaGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGAt                                                      <  1:2389709/150‑1 (MQ=255)

                                                                                                          aGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTg                                             >  1:2822803/1‑150 (MQ=255)

                                                                                                           gCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGc                                            <  2:391482/150‑1 (MQ=255)

                                                                                                            cAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATACCGGCACAGATAATCATCTGcc                                           >  2:3031774/1‑150 (MQ=255)

                                                                                                             aaTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCg                                          >  2:1523119/1‑150 (MQ=255)

                                                                                                              aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCgg                                         <  2:3698461/150‑1 (MQ=255)

                                                                                                              aTTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCgg                                         <  2:3346095/150‑1 (MQ=255)

                                                                                                                    tCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTa                                   >  1:984606/1‑150 (MQ=255)

                                                                                                                     ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAg                                  <  2:2390782/150‑1 (MQ=255)

                                                                                                                     ccAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAg                                  >  1:4129658/1‑150 (MQ=255)

                                                                                                                      cAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGc                                 <  2:1249703/150‑1 (MQ=255)

                                                                                                                       aGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGTTCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCt                                >  1:1281909/1‑150 (MQ=255)

                                                                                                                        gtgtCTCTTTCCCTGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCtt                               <  2:1130622/150‑1 (MQ=255)

                                                                                                                        gtgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCtt                               <  1:2598621/150‑1 (MQ=255)

                                                                                                                        gtgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCtt                               <  1:3209053/150‑1 (MQ=255)

                                                                                                                        gtgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAAAGATTCGCAAATATAATCCAGCGTTTCATTCAACCGCGGTTTGATCATATAGGCACAGATAATCATATGACGGGCTTTATCtt                               >  1:3120024/1‑150 (MQ=255)

                                                                                                                         tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTg                              >  2:3563133/1‑150 (MQ=255)

                                                                                                                         tgtCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTg                              >  1:4052063/1‑150 (MQ=255)

                                                                                                                            ctctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGt                           <  2:2556162/150‑1 (MQ=255)

                                                                                                                             tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTa                          <  1:1523119/150‑1 (MQ=255)

                                                                                                                             tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTa                          >  1:3037219/1‑150 (MQ=255)

                                                                                                                             tctTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTa                          >  2:3358950/1‑150 (MQ=255)

                                                                                                                                    cGGTGAGTACTTTTGCCTCGGCACAATTAGGAAACAGATAATCGACATAACTCAATGCTGCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCAGATCGGCACAGATCATCATCTGCCGGGCTGTAGCTTGCGTACAAAgtt                   >  2:1688001/1‑150 (MQ=255)

                                                                                                                                    cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCCTCTGCCGGGCTTTAGCTTGCGTAAACAttt                   >  2:3749346/1‑150 (MQ=255)

                                                                                                                                    cGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAAttt                   >  1:1005341/1‑150 (MQ=255)

                                                                                                                                     ggagAGTAATATTGCCTCGCCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCACCGATTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTc                  <  2:3016145/147‑1 (MQ=255)

                                                                                                                                      gTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCt                 <  2:4136570/150‑1 (MQ=255)

                                                                                                                                         cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGtt              >  2:1382374/3‑150 (MQ=255)

                                                                                                                                         cctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAAGTTCTGtt              >  2:3081206/3‑150 (MQ=255)

                                                                                                                                          gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTa             <  1:1627464/150‑1 (MQ=255)

                                                                                                                                          gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTa             <  1:286410/150‑1 (MQ=255)

                                                                                                                                          gTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTa             <  1:3776826/150‑1 (MQ=255)

                                                                                                                                          ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTCAAAAGTTCTGttc             >  2:3598234/2‑149 (MQ=255)

                                                                                                                                          ctAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTa             >  2:3510510/2‑150 (MQ=255)

                                                                                                                                           tAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAg            <  2:3006579/150‑1 (MQ=255)

                                                                                                                                           tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAg            >  1:2123038/1‑150 (MQ=255)

                                                                                                                                           tAATTTTGCCGAGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAg            >  2:2903158/1‑150 (MQ=255)

                                                                                                                                            aaTTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAgc           <  1:3798041/150‑1 (MQ=255)

                                                                                                                                               tttGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCt        >  2:353653/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  1:1908548/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  2:33200/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  1:3643022/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  2:500891/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  1:917942/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  1:3151837/1‑150 (MQ=255)

                                                                                                                                                    cTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTAcc   >  2:272552/1‑150 (MQ=255)

CTGCGACCGCCGGCACCTTCATCGTCATGTCACCACGCTTGATAAAGCAGCCGTCTTTACCCGTTTTAATCACCACCGTTTTTACGCCGCACGCAAGAAAGCAGTCAGCAATTTCATCCAGTGTCTCTTTCCCGGTGAGTAATTTTGCCTCGGCAAAATTAGGAAACAGATAATCGACATAACTCAATGCTTCGCAAATATCATCCAGCGTTTCATTCAACCGCGGTTTGATCATATCGGCACAGATAATCATCTGCCGGGCTTTAGCTTGCGTAAAAATTTCTGTTAGCGCTTTACCA  >  NC_000913/1856184‑1856482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`