Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,864,758 G→A 6.8% intergenic (+24/+24) yeaD → / ← yeaE D‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,864,7580GA 6.8% 579.3 / 20.7 175intergenic (+24/+24)yeaD/yeaED‑hexose‑6‑phosphate epimerase‑like protein/aldo‑keto reductase, methylglyoxal to acetol, NADPH‑dependent
Reads supporting (aligned to +/- strand):  ref base G (89/74);  new base A (5/7);  total (94/81)
Fisher's exact test for biased strand distribution p-value = 5.50e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 186 total aligned reads displayed.

GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907
                                                                                                                                                     |                                                                                                                                                     
gATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCggg                                                                                                                                                       >  2:3782582/1‑150 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                     <  1:1038972/150‑1 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                     <  1:1038997/150‑1 (MQ=255)
  tatGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTg                                                                                                                                                     <  1:1038935/150‑1 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCa                                                                                                                                                   >  2:537055/1‑150 (MQ=255)
    tGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCa                                                                                                                                                   >  1:1635900/1‑150 (MQ=255)
     gCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCaa                                                                                                                                                  >  2:1313009/1‑150 (MQ=255)
      ccGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAt                                                                                                                                                 >  1:3687147/1‑150 (MQ=255)
       cGGATGATGGCTACAAAACATTTGTTTTTGTAGAAACGGCTTACGCTACAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAAAGTTGTGTGGCGCGTGCAATg                                                                                                                                                >  2:3822778/1‑150 (MQ=255)
         gatgatGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCa                                                                                                                                              >  2:1878918/1‑150 (MQ=255)
             atgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCg                                                                                                                                          <  1:1755791/150‑1 (MQ=255)
              tgGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCgg                                                                                                                                         <  2:3387044/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTTTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       <  1:723536/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGca                                                                                                                                       >  1:3793067/1‑150 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                       <  1:3529519/150‑1 (MQ=255)
                gCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCATTGCACCCGGca                                                                                                                                       <  1:212447/150‑1 (MQ=255)
                  tACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcaca                                                                                                                                     >  2:3065453/1‑150 (MQ=255)
                   aCAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACAAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacac                                                                                                                                    <  2:3093880/150‑1 (MQ=255)
                    cAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGcacaca                                                                                                                                   >  2:960550/1‑150 (MQ=255)
                      aaaCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaac                                                                                                                                 >  2:961009/1‑150 (MQ=255)
                        aCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACacaacaa                                                                                                                               >  1:3348093/1‑149 (MQ=255)
                        aCATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAt                                                                                                                               >  1:2669295/1‑150 (MQ=255)
                         cATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATc                                                                                                                              >  2:795579/1‑150 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             >  1:2943721/1‑150 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             >  2:2413540/1‑150 (MQ=255)
                          aTTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATca                                                                                                                             <  2:3727958/150‑1 (MQ=255)
                            ttgtttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATcaca                                                                                                                           >  2:208148/1‑150 (MQ=255)
                               tttgtGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCa                                                                                                                        >  2:3598740/1‑150 (MQ=255)
                                 tgtgTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCata                                                                                                                      >  2:3115693/1‑150 (MQ=255)
                                  gtttAGAAACGGCTTACGCTTCAGAAACGAAAAAAGTGACCAACGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                                                                     <  2:1106948/147‑1 (MQ=255)
                                  gtgtAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCatat                                                                                                                     >  2:1644186/1‑150 (MQ=255)
                                    ttAGAAACGGATTACGCTTCATAAACGCAAAAAGTGACCAATTAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCTAAACGTTATTTTACGTTTATGTTTTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATcc                                                                                                                   <  2:1561358/149‑1 (MQ=255)
                                           cGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAg                                                                                                            >  1:1453754/1‑150 (MQ=255)
                                           cGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCACAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAg                                                                                                            >  2:2619492/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCCTATCCAGCGCAGtttg                                                                                                        >  2:4019172/1‑149 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        <  2:2398223/150‑1 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        >  2:1598861/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGtttt                                                                                                        >  2:4127460/1‑150 (MQ=255)
                                               ttACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGtttt                                                                                                        >  1:93194/1‑150 (MQ=255)
                                                  cGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTcc                                                                                                     <  2:2827144/150‑1 (MQ=255)
                                                   gCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCt                                                                                                    >  2:3465842/1‑150 (MQ=255)
                                                     ttCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCGCACCATATCCAGCGCAGTTTTTCCttt                                                                                                  <  2:4124655/150‑1 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                <  2:2043893/150‑1 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGTTg                                                                                                >  2:4133871/1‑150 (MQ=255)
                                                       cAGAAACGCAAAAAGTGACCAAAGAGAAACCGGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTg                                                                                                <  2:1899959/150‑1 (MQ=255)
                                                            ccGCAAAACGTGACCACAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGACACGTTAATTTCCGTTAATGTTGTGTGCCGGGTGCAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGcc                                                                                           <  2:3889529/149‑1 (MQ=255)
                                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACAACACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGat                                                                                       >  1:2823345/1‑150 (MQ=255)
                                                                aaaaaGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGATGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGat                                                                                       >  2:2774071/1‑150 (MQ=255)
                                                                  aatgTGACCATAGATAAACCTCAACATGTGGAGAAACCCTTGCGCGTTGATAATTTTTATTTTACTTTTTTTTTTTGTGCGTGGTTAAATTAATCCGCCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGatat                                                                                     <  1:3014825/147‑1 (MQ=255)
                                                                            acgacaaaCCTGCACATCTGGCGCAATCATTTCGGGTTGCTAAACGTTAATTTACGTTAATTTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCa                                                                           <  1:1289567/145‑1 (MQ=255)
                                                                              gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCGTATCCAGc                                                                         >  1:1075949/1‑150 (MQ=255)
                                                                              gagaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAAATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGc                                                                         >  2:3310550/1‑150 (MQ=255)
                                                                               agaACCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTCCGTTAAGGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                        <  2:13668/150‑1 (MQ=255)
                                                                               agaAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCa                                                                        <  2:2177708/150‑1 (MQ=255)
                                                                                 aaaCCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGACATGCCTTATCCAGCATa                                                                      >  2:1740618/1‑150 (MQ=255)
                                                                                     cTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAAAAAAAAACGCACACAACAACACACCATAACCAGCGCACTTTTTGAATTTGGTGCAGGATATGCCTTAACCAGCATATCTa                                                                  >  1:52747/1‑150 (MQ=255)
                                                                                           aTCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                            <  2:365253/150‑1 (MQ=255)
                                                                                           aTCTGGCGCAATCCAGTCGCGTTGCTAAACGTTAATTTAGTTTATTGTTGTGTGCGGGGTGAAATGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                            <  1:1595071/150‑1 (MQ=255)
                                                                                           aTCGGGCCCAATCCATTCGGGTTGCTAAACTTTAATTTCCTTTATTGTTGTGTGCGGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCg                                                            <  1:3835846/150‑1 (MQ=255)
                                                                                            tCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGc                                                           <  2:868296/150‑1 (MQ=255)
                                                                                             cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          <  1:3034393/150‑1 (MQ=255)
                                                                                             cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          <  2:1046675/150‑1 (MQ=255)
                                                                                             cTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCAGATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCt                                                          <  2:1632815/150‑1 (MQ=255)
                                                                                                 cgcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGGGATGCATCCGGCACACAAAAACACACCATATCCCGCGCAGTTTTTGCTTTTGGTGCCGGATATGGCTTTTCCAGCATATCTAATTACGCTgaag                                                      >  1:818478/1‑150 (MQ=255)
                                                                                                  gcAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTgcaga                                                     >  2:842874/1‑150 (MQ=255)
                                                                                                    aaTCCATTCGCGTTGCTAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGaaa                                                   <  1:2258416/150‑1 (MQ=255)
                                                                                                       ccATTCGCGTTGCTAAACTTTAATTTACTTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGtt                                                <  1:3890247/150‑1 (MQ=255)
                                                                                                          ttCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGa                                             >  2:2521082/1‑150 (MQ=255)
                                                                                                             gcgTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCt                                          <  2:1453754/150‑1 (MQ=255)
                                                                                                                ttGCGAAACGTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCaa                                       <  1:453648/150‑1 (MQ=255)
                                                                                                                ttGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCCGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGATCGACCTCaa                                       >  2:2686306/1‑150 (MQ=255)
                                                                                                                 tGCGAAACTTTAATTTACGTTATTGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAg                                      <  1:2499533/150‑1 (MQ=255)
                                                                                                                     aaaCGTTAATTTACGTTAAGGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAAGTCCGCTGAAGAAAGTTCGACCTCAAGcaca                                  <  2:3666091/150‑1 (MQ=255)
                                                                                                                        cGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGcc                               <  2:3112161/150‑1 (MQ=255)
                                                                                                                        cGTTAATTTACGTTAATGTTGTGTGCCGGGTGAAATGGATCCGGCACACAACATGACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATTTGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGTCGTCTAGCTCAGcc                               <  2:1802781/150‑1 (MQ=255)
                                                                                                                         gTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCGTTTGGTGCCGGATATGCCATATCCAGCCTAGCTAATTCCGCTGAAGAAAGTTCGACCGCAAGCACAGCCg                              >  2:714376/1‑150 (MQ=255)
                                                                                                                          ttAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGc                             >  2:2671938/1‑150 (MQ=255)
                                                                                                                               ttACGTTAATGTTGTGTGCCGGGTGCAATTCATACGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTGCGCTGGAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                        >  2:1576791/1‑150 (MQ=255)
                                                                                                                               ttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                        <  1:2061528/150‑1 (MQ=255)
                                                                                                                               ttACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                        >  2:816166/1‑150 (MQ=255)
                                                                                                                               ttACGTTAATGTTGTGTGCCGGGTGCAATGCAGCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCAtttt                        <  2:3193718/150‑1 (MQ=255)
                                                                                                                                tACGTGAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATCTCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTttg                       <  2:3384059/150‑1 (MQ=255)
                                                                                                                                   gTTAATGTTGTGTGCCGGGGGCAATGCATCCGGCACACAACATCACACCCTATCCAGCGCAGTTTTTCCGGTTGGTGCCGGATATGCCGTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCCCAGCCGCCTTttgttg                    >  2:1938994/1‑150 (MQ=255)
                                                                                                                                     tAATGTTGTGTGCCGGGTGCAATGCATCCGACACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGa                  >  1:303857/1‑150 (MQ=255)
                                                                                                                                          ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg             <  1:195083/150‑1 (MQ=255)
                                                                                                                                          ttgtGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATgg             <  1:3631337/150‑1 (MQ=255)
                                                                                                                                               tgCCGGGTGCAATGCATCCGGCACACAACATCACACCATAACAAGCGCAGTTTTTCCTTTTTGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACCTGGGCAAt        >  2:2477375/1‑150 (MQ=255)
                                                                                                                                               tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCCAGCACAGCCGCATTTTGTTGGACATGGGccag        >  2:2218719/1‑147 (MQ=255)
                                                                                                                                               tgCCGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAAt        >  1:1881935/1‑150 (MQ=255)
                                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:381397/1‑150 (MQ=255)
                                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:1673882/1‑150 (MQ=255)
                                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  2:2938784/1‑150 (MQ=255)
                                                                                                                                                 ccGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTGTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACCTGGGCAATCg      >  2:1853877/1‑150 (MQ=255)
                                                                                                                                                 ccGGATGCATTGCACCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCg      >  1:2122203/1‑150 (MQ=255)
                                                                                                                                                  cGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGt     <  2:3252161/150‑1 (MQ=255)
                                                                                                                                                   gggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTg    <  1:2034856/150‑1 (MQ=255)
                                                                                                                                                    ggTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTgg   <  2:2308567/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  <  2:3545596/150‑1 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  >  2:685306/1‑150 (MQ=255)
                                                                                                                                                     gTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGc  >  2:2311004/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GATATGCCGGATGATGGCTACAAAACATTTGTTTGTGTAGAAACGGCTTACGCTTCAGAAACGCAAAAAGTGACCAAAGAGAAACCTGCACATCTGGCGCAATCCATTCGCGTTGCGAAACGTTAATTTACGTTAATGTTGTGTGCCGGGTGCAATGCATCCGGCACACAACATCACACCATATCCAGCGCAGTTTTTCCTTTTGGTGCCGGATATGCCTTATCCAGCATAGCTAATTCCGCTGAAGAAAGTTCGACCTCAAGCACAGCCGCATTTTGTTGGACATGGGCAATCGTGGC  >  NC_000913/1864609‑1864907

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: