Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,431 G→T 13.4% R434I (AGA→ATA)  gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4310GT13.4% 552.4 / 4.2 205R434I (AGA→ATA) gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (80/88);  new base T (20/4);  total (113/92)
Fisher's exact test for biased strand distribution p-value = 9.56e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 208 total aligned reads displayed.

AGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470282‑2470580
                                                                                                                                                     |                                                                                                                                                      
 gtaTCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGa                                                                                                                                                       >  2:1645282/1‑150 (MQ=255)
    tctcTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                                                                                                    <  2:3911423/150‑1 (MQ=255)
      tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCa                                                                                                                                                  <  2:3936321/150‑1 (MQ=255)
      tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAtaa                                                                                                                                                  >  1:3131032/1‑148 (MQ=255)
      tctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTCAAATaaaaaa                                                                                                                                                  >  2:438186/1‑147 (MQ=255)
       ctTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                                                                                                                 <  1:3332225/150‑1 (MQ=255)
            tGGAATATCTCAATTTTTTGTGTCTTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTTTTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                            <  1:1499210/150‑1 (MQ=255)
            tGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACaa                                                                                                                                            <  1:262152/150‑1 (MQ=255)
               aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAATAAATCAAAACaaaat                                                                                                                                         >  1:2325115/1‑147 (MQ=255)
               aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAAAAAATACAAAAg                                                                                                                                         >  1:3639820/1‑150 (MQ=255)
                   tctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                                                                                                    <  1:1145504/150‑1 (MQ=255)
                     tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTTTTAAATAATTAAAAAAAAAAAACAAAAAAACGATGt                                                                                                                                  >  1:1846600/1‑150 (MQ=255)
                     tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAATAAAAAAACGATGt                                                                                                                                  >  1:2915237/1‑150 (MQ=255)
                     tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAGAAATAAATAAAAAAGCGATGt                                                                                                                                  >  1:3403468/1‑150 (MQ=255)
                     tcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTATTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAAAGCGATGt                                                                                                                                  >  2:2419843/1‑150 (MQ=255)
                      cAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                                                                                                 <  1:1577202/150‑1 (MQ=255)
                         ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                                                                                                              <  2:1124448/150‑1 (MQ=255)
                           ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                                                                                                            <  1:2332718/150‑1 (MQ=255)
                               gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTaa                                                                                                                        <  1:888686/150‑1 (MQ=255)
                                 gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATCAATAAAAAATCGATGTTAAGCACAAAGt                                                                                                                      >  1:1747859/1‑150 (MQ=255)
                                 gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAATCAAGAAAATAGCGATGTAAAGCACAAAGt                                                                                                                      >  2:3989092/1‑150 (MQ=255)
                                  tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGACGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                     <  1:1725313/150‑1 (MQ=255)
                                    aTTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAATAAATAAAGACAATAGCGATGTAAAGCACGAAGTTTa                                                                                                                   >  2:800358/1‑150 (MQ=255)
                                     tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTaa                                                                                                                  >  2:620878/1‑150 (MQ=255)
                                          cGGGGAAGGATATAGAGATCTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgc                                                                                                             <  1:2683322/150‑1 (MQ=255)
                                             ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATAAAATAATTGAAAGAAATACATAAAAAAGCGGTGTGAAGCACGAAGTTGTAATTtcgcg                                                                                                          >  1:2886151/1‑150 (MQ=255)
                                              gAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcgc                                                                                                         <  2:151911/150‑1 (MQ=255)
                                                aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                       <  2:1394804/150‑1 (MQ=255)
                                                 ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCaa                                                                                                      <  1:2037907/150‑1 (MQ=255)
                                                  gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                                                                                                     <  2:3453646/150‑1 (MQ=255)
                                                      taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAATTAAAAAAAAAAAAAAAAAAAGCGATGTAAAGCACAAAGTTTAAATTTCGCGcccaaccgg                                                                                                 >  1:4107140/1‑143 (MQ=255)
                                                        gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc                                                                                               <  1:3225786/150‑1 (MQ=255)
                                                         agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAAAAATAAAGAAAAAAGCGATGTAAAGCAATAAGTTTAAATTTCGCGCCAATCATGgcg                                                                                              >  1:2420231/1‑150 (MQ=255)
                                                          gaTTTAAGCACGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgc                                                                                             <  1:3276111/150‑1 (MQ=255)
                                                           aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAACTCAAGAAAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAACATGgcgcg                                                                                            >  2:334832/1‑150 (MQ=255)
                                                            tttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAAAAAAAAAGAAAATAGCGATGTAAAGCACTAAGTTTTAATTTCGCGCCAATGAAGgcgcgc                                                                                           >  1:2672192/1‑150 (MQ=255)
                                                              tAAGCAATCATTTATTTTGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCATGATTGGCGCGcaa                                                                                         <  2:2133585/150‑2 (MQ=255)
                                                                agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATTAAATAAATAAAAAAAAAAAAAAAAAAATCGATTTAAAACAAAAAAGTTAAATTTAGCGCCAAACAATGCGCGCACaa                                                                                       >  1:3265314/1‑150 (MQ=255)
                                                                agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa                                                                                       <  2:2764574/150‑1 (MQ=255)
                                                                  caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACACGc                                                                                     <  2:1281419/150‑1 (MQ=255)
                                                                  caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                     <  2:579379/150‑1 (MQ=255)
                                                                   aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTAATTTATAAAAAAAATAAAATAAATCAAGACAAAAGCGCTGTAAAGAACTAAGTTTAAAATTCGCGCCCAGCCGTGCGCGCACaaaca                                                                                    >  1:2361320/1‑147 (MQ=255)
                                                                    agcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAAAAAAAAAAAAAGCGATTGTAAACAAAAATATTTAAATTAGCGCCAAACAATGCGCGCACaaacaa                                                                                   >  1:3715668/1‑146 (MQ=255)
                                                                        tttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAAACAACACAAAACCGAAGTAAAGCACTAAGTTTAAATTTCGCGCCCAACATGGCGCCCCCAACCAataaa                                                                               >  2:1963353/1‑149 (MQ=255)
                                                                          tatttGGATTTTATTTTTCTTTCGACCTTTGATTATACATAACTGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATAGCTTTTTAAAGCAATATTTTTAATTTGCGCGCAAATCATGGCGCGCACAAGCTATAATAc                                                                             <  1:3796866/150‑1 (MQ=255)
                                                                              tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAAACAAGAAAAAAGCGCTGTAAATCACTAAGTTTAAAATTCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAAc                                                                         >  2:52811/1‑150 (MQ=255)
                                                                                gAATGTATTTTTCTTTCTACCTTTGCTTATACATAAATGTGTTTTTTTTATTTTATAAAATAATTCAACGAAATCAAGAAAATCGCGTTTTAAACCACTATTTTTAAATGGCGCGCAAATCTTGGCGCGCACAAGCTATAATACCAACCt                                                                       <  1:1290566/150‑1 (MQ=255)
                                                                                  aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                                     <  2:1330576/150‑1 (MQ=255)
                                                                                  aTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTaa                                                                     <  2:698591/150‑1 (MQ=255)
                                                                                        ctcccgctcgACCTCTGCTCACCCATAACCGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGACGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTtctc                                                               <  1:2195978/143‑1 (MQ=255)
                                                                                         tttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                              <  2:3446024/150‑1 (MQ=255)
                                                                                           tcgttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACCAGCTATAATACCAAACAAATTTctcctc                                                            >  2:4139714/1‑150 (MQ=255)
                                                                                              ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCAATAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcctc                                                         >  2:2392540/1‑150 (MQ=255)
                                                                                              ttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATACAATAGCGAAGTAAAGCACTAAGTTTAAATTGCGCGCCAAACATTGCGCGCACAAGCTAAAAAACCAACCTAATTTctccaccac                                                         >  2:2728018/1‑148 (MQ=255)
                                                                                                  aCCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAATGAATCAATAAAATATCGATGTAAATCACTAAGTTTAAAATTCGCGCCAAACAAGGCGCGCACAAGCTATAAAACAAACCAAAATTCTCCTCCACTTat                                                     >  2:88680/1‑149 (MQ=255)
                                                                                                   ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                    <  2:3418220/150‑1 (MQ=255)
                                                                                                   ccTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                    >  2:338699/1‑150 (MQ=255)
                                                                                                   ccTTTGCTTATAAATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATTTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                    <  1:2293194/150‑1 (MQ=255)
                                                                                                        gCTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAATAAATCAAGACAATAGCGATGTAAAGCACTATGTTTAAATGGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                               <  1:1855281/150‑1 (MQ=255)
                                                                                                        gCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGAc                                               <  1:1703230/150‑1 (MQ=255)
                                                                                                         cTTATACATAACAGTCTTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACt                                              <  2:2974101/150‑1 (MQ=255)
                                                                                                         cTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATCAAGACAATAGCGATGTAAAGCACAAAGTTTAAATTTCGCGCCAATCATTGCGCGCACAAGCAATAATACCAAACTAATTTATACTACTCATAGATTGACt                                              >  2:3566682/1‑150 (MQ=255)
                                                                                                          ttATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCAAGGCGCGCACAATCTATAATAACAACCTAATTTCTCCTCCTCTAAGAGTGACta                                             >  2:2704407/1‑150 (MQ=255)
                                                                                                          ttATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAAGAAATCAAGACAAAAGCGATGTAAAGCACTAAGTTTAAATTTCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAACCAAATTTATACTCCTCTTAGAGTTACta                                             >  2:2186822/1‑150 (MQ=255)
                                                                                                           tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACtat                                            <  2:3625531/150‑1 (MQ=255)
                                                                                                           tataCATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAAGACAATAGCGATGTAAAGCACTAAGTTTTAATTTCGCGCCAATAATGGCGCGCACAAGCTATAATAACAACCGAAATTATCCTACACTTAGAGTGACtat                                            >  2:856308/1‑150 (MQ=255)
                                                                                                            ataCATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAATAAAAAATCGATTTAAATCAATAAGTTTAAATTTAGCGCCAATCAATGGGCGCCCCACCGATAAAAACAACCGAAATTATCCTCCTCGTAGAGTGGAtata                                           >  2:3503641/1‑150 (MQ=255)
                                                                                                                aTAACAGTCGTTTTTTTAAATTATAAAAAAATTCAAAGAAAACAAGACAAAAGCGAAGTAAAGCACTAAGTTTAAAATGCGCGCCAAACAAGGCGCGCACAAGCTAAAATACCAACCAAAATTCTCCGCCGCGTAGAGTGACAATAtctc                                       >  2:657882/1‑150 (MQ=255)
                                                                                                                    cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                   <  2:2338967/150‑1 (MQ=255)
                                                                                                                    cAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGa                                   <  1:4133833/150‑1 (MQ=255)
                                                                                                                             ttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTAAAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCATGATAGAAtttc                          >  1:3848036/1‑148 (MQ=255)
                                                                                                                              tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         >  2:2545752/1‑150 (MQ=255)
                                                                                                                              tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  2:3210392/150‑1 (MQ=255)
                                                                                                                              tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                         <  2:3998512/150‑1 (MQ=255)
                                                                                                                                tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                       <  1:1645282/150‑1 (MQ=255)
                                                                                                                                 aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                      >  1:1547428/1‑150 (MQ=255)
                                                                                                                                  aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     >  1:740252/1‑150 (MQ=255)
                                                                                                                                  aTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTAt                     >  2:2733518/1‑150 (MQ=255)
                                                                                                                                   tttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACCAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATCGAATTGCGGTAtt                    >  1:2240838/1‑150 (MQ=255)
                                                                                                                                    ttATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTg                   >  2:3001511/1‑150 (MQ=255)
                                                                                                                                     tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  <  1:957786/150‑1 (MQ=255)
                                                                                                                                     tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                  >  1:132617/1‑150 (MQ=255)
                                                                                                                                      ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                 <  2:2325115/150‑1 (MQ=255)
                                                                                                                                       taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                >  1:3921231/1‑150 (MQ=255)
                                                                                                                                        aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa               <  1:3989092/150‑1 (MQ=255)
                                                                                                                                        aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTa               >  2:1874173/1‑150 (MQ=255)
                                                                                                                                        aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATGGACTa               >  2:2063687/1‑150 (MQ=255)
                                                                                                                                             aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  1:523243/150‑1 (MQ=255)
                                                                                                                                             aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa          <  1:3653811/150‑1 (MQ=255)
                                                                                                                                              atTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAAt         >  1:1535431/1‑150 (MQ=255)
                                                                                                                                                tCAAAGTAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCATGGCGGGCACAAGCTATAATACCAACGTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAATTGc       >  2:3121185/1‑150 (MQ=255)
                                                                                                                                                tCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAAGACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGc       <  2:2886151/150‑1 (MQ=255)
                                                                                                                                                 cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc      >  1:1421340/1‑150 (MQ=255)
                                                                                                                                                  aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  2:3790924/1‑150 (MQ=255)
                                                                                                                                                  aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     <  1:338699/150‑1 (MQ=255)
                                                                                                                                                  aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGAGGTATTGACTATCAAATGccc     >  1:3896752/1‑150 (MQ=255)
                                                                                                                                                  aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGATATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc     >  2:1730753/1‑150 (MQ=255)
                                                                                                                                                   aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCCATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  1:1417887/1‑150 (MQ=255)
                                                                                                                                                   aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:2168924/1‑150 (MQ=255)
                                                                                                                                                   aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt    >  2:3255218/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                      
AGTATCTCTTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGA  >  NC_000913/2470282‑2470580

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: