BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,818,046	A→G	6.0%	intergenic (‑186/+13)	argQ ← / ← argZ	tRNA‑Arg/tRNA‑Arg

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,046	0	A	G	6.0%	533.8 / 14.8	174	intergenic (‑186/+13)	argQ/argZ	tRNA‑Arg/tRNA‑Arg
Reads supporting (aligned to +/- strand): ref base A (100/56); new base G (4/6); total (104/70)
Fisher's exact test for biased strand distribution p-value = 1.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

GCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817897‑2818193

gCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTGGCTAAAGCAACGTTAACATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGa                                                                                                                                                      >  1:1488238/1‑150 (MQ=255)

    ctaAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTc                                                                                                                                                  <  1:3719905/150‑1 (MQ=255)

     taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                 <  1:1475138/150‑1 (MQ=255)

     taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCg                                                                                                                                                 <  1:3318639/150‑1 (MQ=255)

     taAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGATGAAGATTTCg                                                                                                                                                 >  1:2284312/1‑150 (MQ=255)

       aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCGGGCCGCACGCCCTACGGGCCGATGCGAAACCACCGGTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCACTTCGATACCATCCCTGATCAAGATTTCGga                                                                                                                                               >  2:1458006/1‑150 (MQ=17)

       aaGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTAGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGAAACGTTATCATCCCTATTGCCTGCGGTTAATTCCGCTACCTCTTAGATACCTTCATTGATGAAGATTTCGga                                                                                                                                               >  2:2996368/1‑150 (MQ=18)

        aGCCATATCAATTAAGAGGTGGTGCATCCGGGAGGATTACTCGCCTAGCGGCACGCCCTTCTGGCCGTTGCTAAAGCAACGATATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCTATACATTCATTGCTGAAGATTTCGgag                                                                                                                                              >  1:3674706/1‑150 (MQ=17)

               tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                       >  1:1036552/1‑150 (MQ=255)

               tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                       <  1:3870877/150‑1 (MQ=255)

               tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                       >  1:3368517/1‑150 (MQ=255)

               tCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTAGCGGCTCGCCCTTCGGGCCGTTGCGAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATgg                                                                                                                                       >  2:3627227/1‑150 (MQ=255)

                 aaTTAAGTGATTGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                                                                                                     <  1:292297/150‑1 (MQ=255)

                 aaTTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTg                                                                                                                                     >  2:113460/1‑150 (MQ=255)

                   ttAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCa                                                                                                                                   >  2:3265815/1‑150 (MQ=255)

                         gaTGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCggg                                                                                                                             >  2:2557096/1‑150 (MQ=255)

                             gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                         >  2:4104187/1‑150 (MQ=255)

                             gTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGggagga                                                                                                                         >  2:351864/1‑150 (MQ=255)

                                cATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATAGCTGAAGATTTCGGGGAATAGGGTGCAACCGGGAGGAtac                                                                                                                      >  1:2437249/1‑148 (MQ=17)

                                 aTCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCACCGTTATCATCCCTAGGGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGCAGATTTCGGAGAAGATGGTGCATCCGGGAGGATTCg                                                                                                                     >  1:1219578/1‑150 (MQ=255)

                                          gaTTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCTAA‑CCTCCGa                                                                                                           <  2:2372941/150‑1 (MQ=255)

                                            ttACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGG‑TTCGAATcctcccgga                                                                                                         <  1:995673/150‑5 (MQ=18)

                                               cTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCt                                                                                                      >  2:1817023/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                     >  1:2482205/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                     >  1:2490013/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATAATCCCTAGTGCCTGCGGTTAATTACGCTACCTCTTCGATACCTTCATTGCTGAAGATTTAGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCACCGACCGCTc                                                                                                     >  1:1842320/1‑150 (MQ=255)

                                                tCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATAATACCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTc                                                                                                     >  1:950175/1‑150 (MQ=255)

                                                  gCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCgg                                                                                                   >  2:1853796/1‑150 (MQ=255)

                                                     ttGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTc                                                                                                >  2:431637/1‑150 (MQ=255)

                                                           cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGcc                                                                                          >  1:3074332/1‑150 (MQ=255)

                                                           cTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCAGCGGTTAATTCCGCTACCTCTTCGATACCGTCAGTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCGCGGTTCGTAGcc                                                                                          >  1:46135/1‑150 (MQ=255)

                                                            tCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCg                                                                                         <  1:399403/150‑1 (MQ=255)

                                                            tCGCCCTTCGGGCCGTAGCTAAAGCGACGTTATCATCCCTAGTGCCTGCGGTAAATTACGAAACCTCTTCGATACCTTCATTGCTGAAGATTTCGGGGACTATGGTGCATCCGGGAGGATTCGAG‑CCTCCGCCCGCTCGGTTCGTAGCCg                                                                                         >  2:623095/1‑150 (MQ=17)

                                                             cGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGa                                                                                        <  2:297909/150‑1 (MQ=255)

                                                               cccTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGt                                                                                      >  2:1905742/1‑150 (MQ=255)

                                                                     ataacGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                >  2:1981323/5‑150 (MQ=255)

                                                                     ataacGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                >  1:4034776/5‑150 (MQ=255)

                                                                     ataacGTTGCTTTAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTa                                                                                >  2:1981378/5‑150 (MQ=255)

                                                                          gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGTAGATTTCGGTGTATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           >  1:1057512/1‑150 (MQ=255)

                                                                          gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           >  1:3683785/1‑150 (MQ=255)

                                                                          gTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAg                                                                           >  1:2136271/1‑150 (MQ=255)

                                                                           ttGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc                                                                          >  2:1386995/1‑150 (MQ=255)

                                                                             gCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTg                                                                        >  2:1489761/1‑150 (MQ=255)

                                                                               tAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAg                                                                      >  1:578603/1‑150 (MQ=255)

                                                                                 gcgCAACGTTATCCTACCTCGTGCCTGCGGTTAATACCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGa                                                                                        <  1:660233/128‑1 (MQ=11)

                                                                                  aGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTa                                                                   >  1:2696071/1‑150 (MQ=255)

                                                                                   gCAACGTTATCCTTCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGAttt                                                                                                                                                   <  1:302271/70‑1 (MQ=255)

                                                                                    cAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACg                                                                 <  2:893521/150‑1 (MQ=255)

                                                                                    cAACGTTATCATCCCTAGTGCCTGCGGTTAATACCGATAGCTGAACGTAAACTTCAATGGACAGTGTTTGGGAGAAAATGTAGAATACGGGTGGATGCGAA‑ACAAAGAACGCTCGGTTCGTAGCCGTGTACTCTCTGgagctgagctggg                                                                 >  2:1780928/1‑147 (MQ=255)

                                                                                        gTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTTCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATg                                                             >  1:3756300/1‑150 (MQ=255)

                                                                                         ttATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGc                                                            <  2:2692328/150‑1 (MQ=255)

                                                                                             catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                        <  1:527954/150‑1 (MQ=255)

                                                                                             catcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCg                                                        <  1:527804/150‑1 (MQ=255)

                                                                                              atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCgg                                                       >  1:1040938/1‑150 (MQ=255)

                                                                                              atcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTATATCCAGCTGAGCTACGGATGCATCgg                                                       >  2:2428951/1‑150 (MQ=255)

                                                                                               tcCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCggg                                                      >  1:1776017/1‑150 (MQ=255)

                                                                                                cccTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGa                                                     >  2:1103739/1‑150 (MQ=255)

                                                                                                 ccTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaa                                                    >  1:1501881/1‑150 (MQ=255)

                                                                                                  ctcgTGCCTGCGGTTAATACCGCTACCTCGTCGATACCTTCATTGCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                   <  2:3591385/147‑1 (MQ=255)

                                                                                                  cTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGaaa                                                   <  2:3772680/150‑1 (MQ=255)

                                                                                                     gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattt                                                <  2:3027935/150‑1 (MQ=255)

                                                                                                     gTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAattt                                                <  2:1809583/150‑1 (MQ=255)

                                                                                                        ccTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAatttatt                                             >  1:1895700/1‑150 (MQ=255)

                                                                                                         cTGCGGTTAATTCCGCTACCTCTTCGATACCTTAATTGCTGAATATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCGACGGATGCATCGGGAAatttattt                                            >  1:856481/1‑150 (MQ=255)

                                                                                                            cGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAc                                         >  2:780999/1‑150 (MQ=255)

                                                                                                             ggTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                        <  2:3873782/150‑1 (MQ=255)

                                                                                                             ggTTAAGTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCACGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTAct                                        <  2:3874125/150‑1 (MQ=255)

                                                                                                              gctatttCCGCTACCTCTCCGATACCTTCCGTGCTGAAGATTTCTGCGATTTGTGTGGATCCGGGTGGATTCGTA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCTTCGGGAAATTTATTTTActg                                       <  1:1223012/145‑1 (MQ=14)

                                                                                                               ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgc                                      >  1:3293609/1‑150 (MQ=255)

                                                                                                               ttAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCCAA‑CCTCAGACCCCACGGGTAGTGGCCGAGTACTCTATACCGCTGAGCTACGGAGGCATCGGGCGATTTATTTTActgc                                      >  2:3816190/1‑150 (MQ=14)

                                                                                                                tAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgct                                     <  2:1696826/150‑1 (MQ=255)

                                                                                                                 aaTTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTActgctg                                    <  2:2329121/150‑1 (MQ=255)

                                                                                                                   ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGat                                  <  1:2092892/150‑1 (MQ=255)

                                                                                                                   ttCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAACTTAGTTTACTGCAGat                                  >  2:1472440/1‑150 (MQ=255)

                                                                                                                     ccGCTAACTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGatat                                <  1:469986/150‑1 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               >  1:1144005/1‑150 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  1:1167499/150‑1 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  1:2183387/150‑1 (MQ=255)

                                                                                                                      cGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATAtt                               <  1:3503698/150‑1 (MQ=255)

                                                                                                                            ctctTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGAGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGAAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTGCGGATATTGATAcc                         >  2:2183546/1‑150 (MQ=255)

                                                                                                                             tctTCGATACCTTCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCg                        >  1:1807112/1‑150 (MQ=18)

                                                                                                                               ttCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAAGTTATTTTACTGCTGATATTGATACCGct                      >  1:3186091/1‑150 (MQ=255)

                                                                                                                                 cGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGTGCTACGGATGCATGGGGAAATTTATTTTAGTGCTGTTATTGTTAGCGctag                    >  2:3911727/1‑149 (MQ=14)

                                                                                                                                 agATACCTTCATTTCTGAAGTTTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGACGATTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGctac                    <  2:775905/149‑1 (MQ=255)

                                                                                                                                      ccTTCATTGCTGAAGTTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATTCCGCTACTAAAg               <  1:3828636/150‑1 (MQ=255)

                                                                                                                                      ccTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAg               <  1:3828657/150‑1 (MQ=255)

                                                                                                                                        ttCATTGCTGAAGGTTACGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc             >  2:1060481/1‑150 (MQ=18)

                                                                                                                                        ttCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAGTTTACTGCTGATATTGATACCGCTACTAAAGcc             >  1:674226/1‑150 (MQ=255)

                                                                                                                                           aTTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCata          >  2:3074716/1‑150 (MQ=255)

                                                                                                                                            ttGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGcc             >  2:2722869/1‑146 (MQ=255)

                                                                                                                                             tGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATc        <  2:2490013/150‑1 (MQ=255)

                                                                                                                                               cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAATCCATATCaa      >  1:687659/1‑150 (MQ=255)

                                                                                                                                               cTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCaa      <  1:63898/150‑1 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCacg     >  1:4030747/1‑148 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg     <  1:52001/150‑1 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg     >  1:3338009/1‑150 (MQ=255)

                                                                                                                                                tGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAg     >  1:2784076/1‑150 (MQ=255)

                                                                                                                                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGtt                                                                                                 >  1:2318917/1‑56 (MQ=255)

                                                                                                                                                  aaGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTAATTTACTGCTGATATTTATAAAGCTAAAAAAGACAGATCactta   >  2:2120623/1‑146 (MQ=14)

                                                                                                                                                   aGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTaa  <  1:899763/150‑1 (MQ=255)

GCTACTAAAGCCATATCAATTAAGAGATGGTGCATCCGGGAGGATTACTCGCCTTGCGGCTCGCCCTTCGGGCCGTTGCTAAAGCAACGTTATCATCCCTAGTGCCTGCGGTTAATTCCGCTACCTCTTCGATACCTTCATTGCTGAAGATTTCGGAGAATATGGTGCATCCGGGAGGATTCGAA‑CCTCCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTACGGATGCATCGGGAAATTTATTTTACTGCTGATATTGATACCGCTACTAAAGCCATATCAAGTAA  >  NC_000913/2817897‑2818193

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`