Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,904,212 G→A 100% intergenic (+838/+535) ygcE → / ← queE putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,904,2120GA99.5% 1140.7 / ‑5.0 310intergenic (+838/+535)ygcE/queEputative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (170/135);  minor base T (3/1);  total (173/137)
Fisher's exact test for biased strand distribution p-value = 6.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 319 total aligned reads displayed.

GTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTT  >  NC_000913/2904065‑2904356
                                                                                                                                                   |                                                                                                                                                
gTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGacg                                                                                                                                                >  1:3688219/1‑150 (MQ=255)
gTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACGATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGACTACCAAGCCGGGTAGCTGCTCGGTTTATCCCCGCTGacg                                                                                                                                                >  1:745568/1‑150 (MQ=255)
    ggCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCggg                                                                                                                                            <  1:3815794/150‑1 (MQ=255)
     gCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGGTTTCGCAAATCTATGGACTATGGCTCTTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGAGCACGGACTACCAAGCCGGGTATCTGCTCGGTGTATCCCCGCTGTCGCgggg                                                                                                                                           >  2:3887880/1‑150 (MQ=255)
       tCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGaa                                                                                                                                         <  2:1549714/150‑1 (MQ=255)
       tCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTAGGTTTATAACCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCAACGCTGACGCGGGGaa                                                                                                                                         >  1:556636/1‑150 (MQ=255)
          ggTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAacac                                                                                                                                      <  1:77906/150‑1 (MQ=255)
          ggTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATATATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGataaa                                                                                                                                      <  2:2337503/150‑5 (MQ=255)
            tttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGAAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTg                                                                                                                                    <  1:2648918/150‑1 (MQ=255)
             ttATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTgg                                                                                                                                   >  1:3025015/1‑150 (MQ=255)
                 ccccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTTCTATTCGGTTTATCCCCGCTGGCGCGGGGATCACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                               <  2:1634973/150‑1 (MQ=255)
                 ccccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGctct                                                                                                                               <  2:215194/150‑1 (MQ=255)
                  cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTg                                                                                                                              <  2:2085927/150‑1 (MQ=255)
                  cccGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTg                                                                                                                              <  2:3399098/150‑1 (MQ=255)
                  cccGCTGGCGCGGGGAACACCAGTTTTCGCAAATCTATTGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAATACATGTATCTGCTCGGTTTATACCCGCTGAAGCGGGGAACACTGGCTCTg                                                                                                                              >  1:200776/1‑150 (MQ=255)
                     gCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCaa                                                                                                                           <  1:3768641/150‑1 (MQ=255)
                        ggCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcag                                                                                                                        <  1:2066646/150‑1 (MQ=255)
                         gcgcGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagc                                                                                                                       <  2:2922925/150‑1 (MQ=255)
                          cgcgGGGAACACCTGTTTTCTCAAATCTATGGACTATTTCTATTCGGTTTATCCCCGCTGTCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTTCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                      <  1:1188617/150‑1 (MQ=255)
                          cgcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                      >  2:3761788/1‑150 (MQ=255)
                          cgcgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAAcagca                                                                                                                      >  1:3355558/1‑150 (MQ=255)
                            cgGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACagcagc                                                                                                                    <  1:2167366/150‑1 (MQ=255)
                             ggggAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAgcagca                                                                                                                   >  1:2601910/1‑150 (MQ=255)
                                 aaCACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCa                                                                                                               >  1:2773121/1‑150 (MQ=255)
                                  acacCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAt                                                                                                              <  1:3432362/150‑1 (MQ=255)
                                    acCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTATATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGa                                                                                                            >  2:3824055/1‑150 (MQ=255)
                                        gTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCAc                                                                                                        >  2:469320/1‑150 (MQ=255)
                                         ttttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTTTCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCTCCCATGACCACg                                                                                                       >  1:716977/1‑150 (MQ=255)
                                          tttCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGt                                                                                                      >  1:1049711/1‑150 (MQ=255)
                                            tCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCg                                                                                                    <  1:2215601/150‑1 (MQ=255)
                                             cGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCgg                                                                                                   <  2:2399287/150‑1 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGTCGCACGGACTACAAATCCGTGTATGTGCTCGGTTTCTCCGCGCTCACGCGGGGAACACTCGCTCAGCAACAGCAGCACCCATGAGCAGTTCGGt                                                                                                  >  2:2431808/1‑150 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGACTACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  >  2:3926593/1‑150 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGt                                                                                                  >  2:2315380/1‑150 (MQ=255)
                                              gCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGACGGt                                                                                                  >  1:3361902/1‑150 (MQ=255)
                                               cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                 >  1:1583070/1‑150 (MQ=255)
                                               cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                 <  2:1180037/150‑1 (MQ=255)
                                               cAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGtt                                                                                                 >  2:3966402/1‑150 (MQ=255)
                                                     tATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATccc                                                                                           >  2:252956/1‑150 (MQ=255)
                                                      aTGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATcccc                                                                                          >  1:2877068/1‑150 (MQ=255)
                                                       tGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCg                                                                                         >  2:2226436/1‑150 (MQ=255)
                                                        ggACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGc                                                                                        <  1:1980031/150‑1 (MQ=255)
                                                         gACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCt                                                                                       <  1:579618/150‑1 (MQ=255)
                                                          aCTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTg                                                                                      >  2:3126364/1‑150 (MQ=255)
                                                                 cTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                               >  1:2820892/1‑150 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCTGTTTATCCCCGCTGGCGCGGGGaa                                                                            >  1:1274218/1‑150 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            <  1:150674/150‑1 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            <  2:2011566/150‑1 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            >  1:975527/1‑150 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            >  1:3866533/1‑150 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            >  1:420280/1‑150 (MQ=255)
                                                                    ttCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCAAGACCACGTCGGTTTATCCCCGCTGGCGCGGGGaa                                                                            >  1:139776/1‑150 (MQ=255)
                                                                        gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                        >  1:2390213/1‑150 (MQ=255)
                                                                        gTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCCGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACg                                                                        >  2:2757806/1‑150 (MQ=255)
                                                                         tttATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGa                                                                       >  1:3043497/1‑150 (MQ=255)
                                                                            aTCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAAt                                                                    >  2:765104/1‑150 (MQ=255)
                                                                             tCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATg                                                                   >  1:3651690/1‑150 (MQ=255)
                                                                               cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCt                                                                 <  2:401951/150‑1 (MQ=255)
                                                                               cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCt                                                                 >  1:911109/1‑150 (MQ=255)
                                                                               cccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCt                                                                 >  1:3940369/1‑150 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGGCCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                <  2:682261/150‑1 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                <  1:4133972/150‑1 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                >  2:1793056/1‑150 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                >  2:3694235/1‑150 (MQ=255)
                                                                                ccGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTg                                                                <  2:3473654/150‑1 (MQ=255)
                                                                                   cTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGt                                                              >  2:5441/1‑149 (MQ=255)
                                                                                    tGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGGTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGGCCACGGCGGTTTATCCCCGCGGGCGCGGGGCCCACGTACTGCGGGTGa                                                            >  2:169004/1‑150 (MQ=255)
                                                                                       cgcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGTAATGCTGGTGAGCg                                                         >  1:579026/1‑150 (MQ=255)
                                                                                        gcgGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGt                                                        <  2:3269127/150‑1 (MQ=255)
                                                                                          ggggAACACGGGCGCACGGAATACACAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTa                                                      <  1:1304126/150‑1 (MQ=255)
                                                                                            ggAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAAt                                                    <  1:3634794/150‑1 (MQ=255)
                                                                                             gAACACGGGCGCACGGAATACAAAGCCGCGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATg                                                   <  1:599510/150‑1 (MQ=255)
                                                                                               acacGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGcc                                                 >  1:1160085/1‑150 (MQ=255)
                                                                                                     gcgcACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCggg                                                                               >  2:899272/1‑114 (MQ=255)
                                                                                                        cACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAAcaca                                        <  2:3039100/150‑1 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       <  2:2820892/150‑1 (MQ=255)
                                                                                                         aCGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAg                                       <  1:1805660/150‑1 (MQ=255)
                                                                                                          cGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAgg                                      <  2:401174/150‑1 (MQ=255)
                                                                                                           ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt                                     <  2:2671530/150‑1 (MQ=255)
                                                                                                           ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt                                     <  2:1870978/150‑1 (MQ=255)
                                                                                                           ggAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGt                                     <  2:2047421/150‑1 (MQ=255)
                                                                                                                aCAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCATCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATc                                <  2:1112062/150‑1 (MQ=255)
                                                                                                                   aaGCCGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATcccc                             <  2:1242044/150‑1 (MQ=255)
                                                                                                                       cGTGTATCTGCTCGGTTTCTCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGAGCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTg                         <  1:3144435/150‑1 (MQ=255)
                                                                                                                       cGTGTATCTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTg                         >  1:3512965/1‑150 (MQ=255)
                                                                                                                              cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                  <  2:3602315/150‑1 (MQ=255)
                                                                                                                              cTGCTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCggg                  <  1:3193205/150‑1 (MQ=255)
                                                                                                                                 cTCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACCGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGCACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTGTATCCCCACTGGCGCggggcc               >  2:1067045/1‑148 (MQ=255)
                                                                                                                                  tCGGTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAac              <  2:3385841/150‑1 (MQ=255)
                                                                                                                                  tCGGTTTATCCCCGCTGACGCGGGGAACAATGGCTCTTCAACAGCAGCACCCATGACCACGTCGGTTTATCACCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGATTATCCCCGCTGGCGCGGGGAac              >  1:947808/1‑150 (MQ=255)
                                                                                                                                    ggTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAacac            <  1:3268678/150‑1 (MQ=255)
                                                                                                                                     gTTTATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAAcaca           >  2:600389/1‑150 (MQ=255)
                                                                                                                                      tttATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACAt          <  1:4036505/150‑1 (MQ=255)
                                                                                                                                        tATCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTa        <  2:140031/150‑1 (MQ=255)
                                                                                                                                         aTCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTAc       >  2:3192216/1‑150 (MQ=255)
                                                                                                                                          tCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACg      <  1:1695989/150‑1 (MQ=255)
                                                                                                                                          tCCCCGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACg      >  1:3166508/1‑150 (MQ=255)
                                                                                                                                           ccccGCTGACGCGGGGAACACTGGATCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGc     <  1:482524/150‑1 (MQ=255)
                                                                                                                                            cccGCTGACGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGcc    >  1:1000864/1‑150 (MQ=255)
                                                                                                                                             ccGCTGACGCGGGGAACACTGGCTATGCAACAGCAGCACCCATGAACACGTCGGGTTATCCCCGCTGTCGCGGGGACCCCGAAATGCTGGTGCGCGTTAATGCCGCAAACGCAGGTTGATCCCCGCTGGCGCGGGGAACACGTTACGCCt   >  1:2792169/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
GTCAGGCTCCGGTTTATCCCCGCTGGCGCGGGGAACACCTGTTTTCGCAAATCTATGGACTATTGCTATTCGGTTTATCCCCGCTGGCGCGGGGAACACGGGCGCACGGAATACAAAGCCGTGTATCTGCTCGGTTTATCCCCGCTGGCGCGGGGAACACTGGCTCTGCAACAGCAGCACCCATGACCACGTCGGTTTATCCCCGCTGGCGCGGGGAACACGAAATGCTGGTGAGCGTTAATGCCGCAAACACAGGTTTATCCCCGCTGGCGCGGGGAACACATTACGCCTT  >  NC_000913/2904065‑2904356

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: