Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,239,609 T→G 6.4% intergenic (+64/‑335) alx → / → sstT putative membrane‑bound redox modulator/sodium:serine/threonine symporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,239,6090TG 6.4% 572.9 / 16.9 191intergenic (+64/‑335)alx/sstTputative membrane‑bound redox modulator/sodium:serine/threonine symporter
Reads supporting (aligned to +/- strand):  ref base T (121/49);  new base G (3/10);  total (124/67)
Fisher's exact test for biased strand distribution p-value = 8.12e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 195 total aligned reads displayed.

TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757
                                                                                                                                                      |                                                                                                                                                    
    cGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                                                                                                                                                  <  1:3945031/150‑1 (MQ=255)
    cGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAg                                                                                                                                                  <  1:1936079/150‑1 (MQ=255)
     gtggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAgg                                                                                                                                                 <  1:3269369/150‑1 (MQ=255)
       ggtgTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGga                                                                                                                                               <  2:1775926/150‑1 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGgag                                                                                                                                              <  1:3141187/150‑1 (MQ=255)
        gtgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGgag                                                                                                                                              <  1:1875969/150‑1 (MQ=255)
         tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             <  2:520702/150‑1 (MQ=255)
         tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             <  2:3809233/150‑1 (MQ=255)
         tgtTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGgaga                                                                                                                                             >  1:1974873/1‑150 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           <  1:1118251/150‑1 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAgg                                                                                                                                           >  1:3726733/1‑150 (MQ=255)
           tttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTGGAAGGGAGAgg                                                                                                                                           <  2:3781738/150‑1 (MQ=255)
            ttGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAggg                                                                                                                                          <  1:2753134/150‑1 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         <  1:257100/150‑1 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGt                                                                                                                                         <  2:2101896/150‑1 (MQ=255)
             tGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCACCCTCTCCCTGGAAGGGAGAggga                                                                                                                                         <  2:3892425/150‑2 (MQ=255)
              ggCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTc                                                                                                                                        >  2:2681976/1‑150 (MQ=255)
               gCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCg                                                                                                                                       <  2:779926/150‑1 (MQ=255)
                cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                      >  1:1374209/1‑150 (MQ=255)
                cATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgg                                                                                                                                      <  1:3982543/150‑1 (MQ=255)
                  ttCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCgggg                                                                                                                                    >  2:4036587/1‑150 (MQ=255)
                   tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAGCATGAGCACCGTTTTCTCCCTCGCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                   <  2:4111193/150‑1 (MQ=255)
                   tCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGt                                                                                                                                   <  2:229948/150‑1 (MQ=255)
                     tGGTGATGCCGTTTATTATCAACGCCTGGGTGAATGATCGGCATGATACGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGa                                                                                                                                 <  2:1468035/150‑1 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 >  1:1548752/1‑150 (MQ=255)
                     tGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGa                                                                                                                                 <  2:2148339/150‑1 (MQ=255)
                      ggTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAg                                                                                                                                >  2:3536013/1‑150 (MQ=255)
                            gaCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATACGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGATTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAt                                                                                                                          >  2:3223042/1‑150 (MQ=255)
                             aCGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAACTTTTAATCTGCCTAAGCCGTGTACCCTGTCACTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtt                                                                                                                         >  1:98312/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  1:2385688/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  1:1693782/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  2:3488060/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  2:3407523/1‑150 (MQ=255)
                               gTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAtttt                                                                                                                       >  2:2816703/1‑150 (MQ=255)
                                tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      >  1:3835203/1‑150 (MQ=255)
                                tttATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAttttt                                                                                                                      >  1:3968465/1‑150 (MQ=255)
                                       tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                               >  1:2555215/1‑150 (MQ=255)
                                       tCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCg                                                                                                               >  2:1724074/1‑150 (MQ=255)
                                        cAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGa                                                                                                              >  2:1851328/1‑150 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATCAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                             >  2:1356585/1‑150 (MQ=255)
                                         aaCGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGAt                                                                                                             >  1:4041601/1‑150 (MQ=255)
                                               tGGGTGAATTATCGGTATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGc                                                                                                       >  2:2702143/1‑150 (MQ=255)
                                               tGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATTCTGGc                                                                                                       >  2:3992068/1‑150 (MQ=255)
                                                            ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                          >  2:3092609/1‑150 (MQ=255)
                                                            ggCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAc                                                                                          >  1:1615002/1‑150 (MQ=255)
                                                             gCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCAcc                                                                                         >  1:228426/1‑150 (MQ=255)
                                                                 gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTTTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                     <  2:1019373/150‑1 (MQ=255)
                                                                 gATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTaa                                                                                     >  2:2000093/1‑150 (MQ=255)
                                                                   tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                   >  1:2895255/1‑150 (MQ=255)
                                                                   tAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTGTCTCCCTCCCCCTCCCTGGGATAGGGTCGGGGTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAAcc                                                                                   <  1:3031629/150‑1 (MQ=39)
                                                                                 gATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCACCCCAAACgg                                                                     >  2:1438801/1‑150 (MQ=255)
                                                                                   tattttttAATCTGCCTACGCCGTGTACCCTGAAATTAACATGAGCACCTTTTTCTCCCCCCCCCTACCCGGGCGTGGGTGGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   <  2:3943990/147‑1 (MQ=25)
                                                                                   aaaTTTTTAATCTCCATAAGCCGTTAACCCTGTAATTAACATTACCACCTTTTTCCCCCTCACCCTACCCGGTAGAGGCTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACgggg                                                                   <  1:2030272/149‑1 (MQ=21)
                                                                                       ttttAATCTGCCTAAGCCGTGTACCCTGTCTTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGTGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAg                                                               <  2:2058799/150‑1 (MQ=255)
                                                                                        tttAATCTGCCTAGGCGGTGCACCCTTTAATTAACTTGCGCCCCGTTTTCCCCCGCCCCCTGCGCGGGTGTGGGGGGGGTTGTGGGAAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                              <  1:2439267/150‑1 (MQ=16)
                                                                                        tttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAgg                                                              >  1:1189117/1‑150 (MQ=255)
                                                                                         ttAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                             >  2:2282382/1‑150 (MQ=255)
                                                                                         ttAATCTCCATAAGCGTTTAACCCTGACTTAACATTGAGCACGGTTTCCCCCCTCCCCCTCCCGGGTTGTGGGTGGGGTTTTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                             <  1:3663114/150‑1 (MQ=12)
                                                                                         ttAATCGGCCTACGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCGCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAggg                                                             <  2:2421012/150‑1 (MQ=255)
                                                                                           aaTCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGGGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCCGAGGGGa                                                           <  2:671206/150‑1 (MQ=255)
                                                                                            aTCCGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGAc                                                          >  2:4006678/1‑150 (MQ=255)
                                                                                             tCTGCCTAAGCCGTGTACCCTGTCATTATCATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACt                                                         >  1:3507548/1‑150 (MQ=255)
                                                                                              cTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTg                                                        >  1:1411323/1‑150 (MQ=255)
                                                                                                  cTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCg                                                    >  1:1990000/1‑150 (MQ=255)
                                                                                                   tAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCACACCCCAAACGGGGCGAGGGGACTGACCGa                                                   >  1:1817218/1‑150 (MQ=255)
                                                                                                    acgCCGTGAACCCTTTCATTAACTTGAGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                  <  1:499476/148‑1 (MQ=255)
                                                                                                    aaGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAg                                                  >  2:2292540/1‑150 (MQ=255)
                                                                                                          tgtACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCttg                                            >  2:3558619/1‑149 (MQ=255)
                                                                                                             ccccTTTAATTAACATGTGCACGGTTTTCTCCCTCTCCGTTCCCGGGTTTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCtttttt                                         <  2:2554253/149‑1 (MQ=25)
                                                                                                               ccTTTCATTAACTTGTGCACCGTTTTCTCCCTCTCCCTTCCCGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGa                                       <  2:4101139/150‑1 (MQ=25)
                                                                                                               ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGGTGGCCTGTTCCCCTCACCCTAACCCACTCCCCGAACGGGGCGATGGGACTGACCGAGTCCTTTTTTGa                                       >  2:308324/1‑150 (MQ=255)
                                                                                                               ccTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTGTTTTgt                                       >  1:1579869/1‑149 (MQ=255)
                                                                                                                cTGTCATTAACATGAGCACCGTTTACTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                      <  2:3731424/150‑1 (MQ=255)
                                                                                                                cTGACATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTGCGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAt                                      <  2:3457445/150‑1 (MQ=255)
                                                                                                                 tatCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCATGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtg                                     >  1:2673210/3‑150 (MQ=255)
                                                                                                                     aTTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGAtgttgt                                 >  1:3360074/1‑150 (MQ=255)
                                                                                                                      ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGTGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                <  1:4076485/150‑1 (MQ=255)
                                                                                                                      ttAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAAATTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGACTCCTTTTTTGATGTTGtc                                >  2:3234815/1‑150 (MQ=255)
                                                                                                                      ttAACATGAGCACCGTTTTCGCCCACTCCCTTCCCGGGAGAGGGTGGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtc                                <  2:3400795/150‑1 (MQ=255)
                                                                                                                       tAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGtca                               >  1:2941131/1‑150 (MQ=255)
                                                                                                                           aTGAGCACCGTTTTCTCCCTCCCCCTCCCCGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAg                           <  1:1033436/150‑1 (MQ=255)
                                                                                                                               aGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCt                        >  2:1661651/1‑150 (MQ=255)
                                                                                                                                gCACCGTTTTCTCCCTCTCCCTCCCTGGGTTTGGTTCGGTTTGTGGTTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                       <  1:3443767/150‑1 (MQ=37)
                                                                                                                                gCACCGTTTTCTCCCTCTCCCGGCCCGGGAGAGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTg                       <  2:249765/150‑1 (MQ=255)
                                                                                                                                          ctccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCAAACCCACTCCCCCAACGGGGCGAGGGGACTGACCGCGTCCATTTTTTGTGTTGTCATCAGTCTGGAAGCCGCAc             >  1:711009/1‑150 (MQ=255)
                                                                                                                                           tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            >  1:2101743/1‑150 (MQ=255)
                                                                                                                                           tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            >  1:2662560/1‑150 (MQ=255)
                                                                                                                                           tccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACg            >  1:3605497/1‑150 (MQ=255)
                                                                                                                                            ccctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCGAACCCTCTCCCCACACGGGGCGAGGGGACTGACCGAGTCCGTTTTTGATGGTGTCATCAGTCTGGAAGCCGCACGt           >  2:1180635/1‑150 (MQ=255)
                                                                                                                                             cctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGtt          >  2:366870/1‑150 (MQ=255)
                                                                                                                                             cctctccctGGAAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTACCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTTATGTTGTCATCAGTCAGGAAGCCGCACGtt          >  2:419829/1‑150 (MQ=37)
                                                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         >  1:3111199/1‑150 (MQ=255)
                                                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         >  1:3115128/1‑150 (MQ=255)
                                                                                                                                              ctctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCACACCCTAACCCACTCCCCAAACGGGGCGAGGGGACTGACCGAGTACTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTg         >  1:919608/1‑150 (MQ=255)
                                                                                                                                                ctccctTCCCGGGAGTGGGTCGGGGTGTGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc       <  2:347755/150‑1 (MQ=255)
                                                                                                                                                ctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCACCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGc       >  2:1217016/1‑150 (MQ=255)
                                                                                                                                                ctccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCGCACCCAAACCCGCTCCCCCAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGGTGGc       >  1:1072649/1‑150 (MQ=255)
                                                                                                                                                 tccctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCt      >  2:3124422/1‑150 (MQ=255)
                                                                                                                                                   cctTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCttt    >  2:929153/1‑150 (MQ=255)
                                                                                                                                                     ttCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACAGATGCTGGCCTGTTCCCCTCACCCTAACCCTCACCCCAAACGGGGCGAGGGGACTGACAGAGTCCTATTTTGATGTTGTCAACAGTATGGAAGCCGAACGTTGGCTTTAt  >  2:1263634/1‑150 (MQ=37)
                                                                                                                                                      |                                                                                                                                                    
TGGGCGTGGTGTTTGGCATTCTGGTGATGACGTTTATTATCAACGCCTGGGTGAATTATCGGCATGATAAGCAGCGGGGTGGATAATTTTTAATCTGCCTAAGCCGTGTACCCTGTCATTAACATGAGCACCGTTTTCTCCCTCTCCCTTCCAGGGAGAGGGTCGGGGTGAGGGTAATTTTTCGCACCGATGCTGGCCTGTTCCCCTCACCCTAACCCTCTCCCCAAACGGGGCGAGGGGACTGACCGAGTCCTTTTTTGATGTTGTCATCAGTCTGGAAGCCGCACGTTGGCTTTAT  >  NC_000913/3239460‑3239757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: