Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,332,823 T→C 5.0% intergenic (‑88/+39) yhbE ← / ← rpmA EamA family inner membrane putative transporter/50S ribosomal subunit protein L27

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,332,8230TC 5.0% 878.5 / 23.5 258intergenic (‑88/+39)yhbE/rpmAEamA family inner membrane putative transporter/50S ribosomal subunit protein L27
Reads supporting (aligned to +/- strand):  ref base T (153/92);  new base C (6/7);  total (159/99)
Fisher's exact test for biased strand distribution p-value = 2.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.89e-01

Only 100 of 266 total aligned reads displayed.

GCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTT  >  NC_000913/3332674‑3332972
                                                                                                                                                     |                                                                                                                                                     
   ccccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGtt                                                                                                                                                    <  2:2385556/150‑1 (MQ=255)
    cccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTg                                                                                                                                                   >  1:2521066/1‑150 (MQ=255)
    cccAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTg                                                                                                                                                   >  1:1413615/1‑150 (MQ=255)
        gCATATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                               <  1:3481459/150‑1 (MQ=255)
        gCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCggg                                                                                                                                               <  1:1175722/150‑1 (MQ=255)
        gCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCggg                                                                                                                                               <  1:459355/150‑1 (MQ=255)
         cAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCgggg                                                                                                                                              <  2:3587990/150‑1 (MQ=255)
          aaaTTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACACGTTGCGGGGc                                                                                                                                             <  2:1853650/150‑1 (MQ=255)
               gCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCa                                                                                                                                        <  2:1897251/150‑1 (MQ=255)
                  gtggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATcc                                                                                                                                     <  1:1330414/150‑1 (MQ=255)
                   tggtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCg                                                                                                                                    >  2:3987312/1‑150 (MQ=255)
                     gtgAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGtt                                                                                                                                  >  2:3510618/1‑150 (MQ=255)
                          cgcCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCgg                                                                                                                             <  2:1420372/150‑1 (MQ=255)
                              aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcg                                                                                                                         >  1:2324368/1‑150 (MQ=255)
                              aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGAcg                                                                                                                         >  2:3077297/1‑150 (MQ=255)
                              aaaaGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTGACCGGGAcg                                                                                                                         >  1:3935839/1‑150 (MQ=255)
                                     tgccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGaaaaa                                                                                                                  >  1:3268524/1‑150 (MQ=255)
                                       ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACt                                                                                                                >  1:900015/1‑150 (MQ=255)
                                       ccaatgccTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACt                                                                                                                <  2:808848/150‑1 (MQ=255)
                                             cctgcctgCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCa                                                                                                          <  2:2778782/150‑1 (MQ=255)
                                                  ctgctgCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTc                                                                                                     >  2:2757836/1‑150 (MQ=255)
                                                    gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa                                                                                                   <  1:2116894/150‑1 (MQ=255)
                                                    gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa                                                                                                   >  1:3224101/1‑150 (MQ=255)
                                                    gctgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCAGGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGa                                                                                                   >  1:3159894/1‑150 (MQ=255)
                                                      tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATg                                                                                                 <  1:3713758/150‑1 (MQ=255)
                                                      tgctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATg                                                                                                 <  1:1596360/150‑1 (MQ=255)
                                                      tgctTAATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATg                                                                                                 >  2:2469993/1‑150 (MQ=255)
                                                        ctTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCt                                                                                               >  2:2290128/1‑150 (MQ=255)
                                                         ttCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTg                                                                                              <  1:257138/150‑1 (MQ=255)
                                                         ttCATACCGGATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTg                                                                                              <  2:2773563/150‑1 (MQ=255)
                                                             tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAATGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaa                                                                                          >  1:6944/1‑150 (MQ=255)
                                                             tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaa                                                                                          >  1:526726/1‑150 (MQ=255)
                                                             tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaa                                                                                          >  1:1019777/1‑150 (MQ=255)
                                                             tACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATaa                                                                                          >  1:6934/1‑150 (MQ=255)
                                                               ccGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAt                                                                                        <  2:3873437/150‑1 (MQ=255)
                                                               ccGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAAt                                                                                        >  2:264284/1‑150 (MQ=255)
                                                                  tATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTa                                                                                     >  1:2419743/1‑150 (MQ=255)
                                                                       ccTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGtt                                                                                >  2:559286/1‑150 (MQ=255)
                                                                         tGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGtttt                                                                              >  2:2153808/1‑150 (MQ=255)
                                                                           cAAAGCGCATTTGCGGATTAACGTCGCACCCTGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTc                                                                            >  1:369308/1‑150 (MQ=255)
                                                                              aGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCggg                                                                         <  1:1818073/150‑1 (MQ=255)
                                                                               gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                        >  2:4050441/1‑150 (MQ=255)
                                                                               gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                        >  2:2821842/1‑150 (MQ=255)
                                                                               gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                        >  1:3595647/1‑150 (MQ=255)
                                                                               gcgcATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                        >  1:3389281/1‑150 (MQ=255)
                                                                                   aTTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCttt                                                                    >  1:2944523/1‑150 (MQ=255)
                                                                                    tttGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTa                                                                   >  1:3980940/1‑150 (MQ=255)
                                                                                       gCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGc                                                                        <  2:3683008/142‑1 (MQ=255)
                                                                                       gCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACt                                                                >  1:1424690/1‑150 (MQ=255)
                                                                                         ggATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTc                                                              <  1:1210221/150‑1 (MQ=255)
                                                                                          gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg                                                             >  1:929879/1‑150 (MQ=255)
                                                                                          gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg                                                             >  2:3001615/1‑150 (MQ=255)
                                                                                          gATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCg                                                             >  2:1372774/1‑150 (MQ=255)
                                                                                            ttAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGaa                                                           <  1:2694656/150‑1 (MQ=255)
                                                                                             tAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAt                                                          >  1:435017/1‑150 (MQ=255)
                                                                                               aCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAttt                                                        >  1:504550/1‑150 (MQ=255)
                                                                                               aCGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAttt                                                        >  1:2062683/1‑150 (MQ=255)
                                                                                                cGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCGTCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTc                                                       <  2:3706906/150‑1 (MQ=255)
                                                                                                 gTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCa                                                      >  1:3342292/1‑150 (MQ=255)
                                                                                                 gTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTAATTCAGCTTCGATGCTGATAAATATACGGTTTTTCGGGCCTTTAACTTCGAATTTCa                                                      >  1:2241959/1‑150 (MQ=255)
                                                                                                    gCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACtt                                                   >  1:1936562/1‑150 (MQ=255)
                                                                                                     cACCATGCGCATTTTTATCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACttt                                                  >  2:210199/1‑150 (MQ=255)
                                                                                                      aCCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCATCGTGTTGCTGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTTACTTCGAATTTCACTTTa                                                 <  2:3124861/150‑1 (MQ=255)
                                                                                                        cATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTAcc                                               >  1:2616075/1‑150 (MQ=255)
                                                                                                           gcgcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTc                                            >  2:3346058/1‑150 (MQ=255)
                                                                                                           gcgcATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTc                                            <  1:3089675/150‑1 (MQ=255)
                                                                                                                 ttttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTTCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCttt                                      >  1:3149611/1‑150 (MQ=255)
                                                                                                                  tttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTa                                     <  1:165692/150‑1 (MQ=255)
                                                                                                                   ttACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAg                                    >  2:2458422/1‑150 (MQ=255)
                                                                                                                       cGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCaaa                                <  1:3863627/150‑1 (MQ=255)
                                                                                                                        ggTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAAc                               >  2:2696700/1‑150 (MQ=255)
                                                                                                                         gtaaTCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCAACCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAAcc                              >  2:150561/4‑149 (MQ=255)
                                                                                                                         gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACa                              >  1:3304248/1‑150 (MQ=255)
                                                                                                                         gTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACa                              >  1:477029/1‑150 (MQ=255)
                                                                                                                           tATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACaga                            <  1:1978302/150‑1 (MQ=255)
                                                                                                                             tCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAgt                          >  2:3442085/1‑150 (MQ=255)
                                                                                                                               gAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAgtgt                        >  2:2319069/1‑150 (MQ=255)
                                                                                                                               gAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAgtgt                        >  1:3196369/1‑150 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAACACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       <  2:2961147/150‑1 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       <  1:2868309/150‑1 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       <  2:2007823/150‑1 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       >  2:1658413/1‑150 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       <  1:1502315/150‑1 (MQ=255)
                                                                                                                                aaTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGtgtg                       <  1:4089129/150‑1 (MQ=255)
                                                                                                                                 aTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTgg                      <  2:1346727/150‑1 (MQ=255)
                                                                                                                                 aTGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAAGTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTgg                      <  2:538310/150‑1 (MQ=255)
                                                                                                                                      gaaaGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACg                 >  2:1072023/2‑150 (MQ=255)
                                                                                                                                         aGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAcc              >  2:2951331/1‑150 (MQ=255)
                                                                                                                                         aGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAcc              >  2:3774970/1‑150 (MQ=255)
                                                                                                                                         aGCCCCGCAACACGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGAcc              >  1:812001/1‑150 (MQ=255)
                                                                                                                                             ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCaa          >  2:1532950/1‑150 (MQ=255)
                                                                                                                                             ccGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCaa          >  1:1290927/1‑150 (MQ=255)
                                                                                                                                             ccGAAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCGCTTTACCGTCTGCTTTAGCCAACCGAGTGTGGTACCGACCGCaa          >  2:1640518/1‑150 (MQ=255)
                                                                                                                                              cGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAAc         >  1:3507502/1‑150 (MQ=255)
                                                                                                                                              cGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAAc         >  1:1440965/1‑150 (MQ=255)
                                                                                                                                                cAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCt       >  2:51948/1‑150 (MQ=255)
                                                                                                                                                    gtgtTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGt   <  1:2205638/150‑1 (MQ=255)
                                                                                                                                                    gtgtTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGt   <  1:2918561/150‑1 (MQ=255)
                                                                                                                                                    gtgtTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGt   <  1:1711109/150‑1 (MQ=255)
                                                                                                                                                     tgtTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGtt  >  1:1148826/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCCCCCCAGCAAATTGCTGTGGTGAGCGCCAAAAGAATGCCAATGCCTGCCTGCTGCTTCATACCGTATTCCCTGCAAAGCGCATTTGCGGATTAACGTCGCACCATGCGCATTTTTACCGGTTATCGAATGTAAAAAGCCCCGCAACGTGTTGCGGGGCTTTCATCCGTTACCGGGACGCGAAAAACTTATTCAGCTTCGATGCTGATAAATTTACGGTTTTTCGGGCCTTTAACTTCGAATTTCACTTTACCGTCTGCTTTAGCAAACAGAGTGTGGTCACGACCGCAACCTACGTT  >  NC_000913/3332674‑3332972

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: