Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,504,855 T→G 6.5% intergenic (+72/+80) frlR → / ← yhfS putative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,504,8550TG 6.5% 427.8 / 2.3 165intergenic (+72/+80)frlR/yhfSputative DNA‑binding transcriptional regulator/FNR‑regulated pyridoxal phosphate‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base T (56/93);  new base G (3/12);  total (60/105)
Fisher's exact test for biased strand distribution p-value = 2.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 169 total aligned reads displayed.

GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504706‑3505004
                                                                                                                                                     |                                                                                                                                                     
   aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTcc                                                                                                                                                    >  1:2359665/1‑150 (MQ=255)
   aaaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCAACGCATCCGGCGATGCTGGCGCGGTGAATTTTACATCCCGTACGttac                                                                                                                                                    >  1:3498850/1‑148 (MQ=255)
    aaactttGCACGTCTCAGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACAGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                   <  2:1788509/144‑1 (MQ=255)
    aaCCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTccc                                                                                                                                                   <  1:1640235/150‑1 (MQ=255)
           gCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCAccc                                                                                                                                            >  2:2798454/1‑150 (MQ=255)
             aCGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAcccaa                                                                                                                                          >  1:1294314/1‑148 (MQ=255)
               gTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAc                                                                                                                                        <  1:3859184/150‑1 (MQ=255)
                tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  2:857862/150‑1 (MQ=255)
                tctcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAcc                                                                                                                                       <  1:1493072/150‑1 (MQ=255)
                  tcCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACAATTCATAAAAAAAGACATAAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCGaaacaa                                                                                                                                     >  2:3188865/1‑146 (MQ=38)
                     gAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTGGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAaccacac                                                                                                                                  >  1:3782236/1‑146 (MQ=255)
                      aaCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGGCCGCACCCAGAcccacaac                                                                                                                                 >  1:1063729/1‑145 (MQ=38)
                       aCTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTccc                                                                                                                                <  2:639587/150‑1 (MQ=255)
                           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCCGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCCCCCCAACCCACAcccccca                                                                                                                            >  1:451683/1‑147 (MQ=39)
                           ttctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCaaa                                                                                                                            <  1:2423428/150‑1 (MQ=255)
                            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATATGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           <  2:2543755/150‑1 (MQ=255)
                            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAg                                                                                                                           >  2:4001007/1‑150 (MQ=255)
                            tctGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCTAACCCACTCCCCCAAg                                                                                                                           >  2:3994090/1‑150 (MQ=255)
                               gCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCCAAgggg                                                                                                                        <  2:3402964/150‑1 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTCCCCAAAGGGGc                                                                                                                       >  2:2642051/1‑150 (MQ=255)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCACCCCACCCAAACCCACACACCCAATGGGc                                                                                                                       >  1:3611639/1‑150 (MQ=38)
                                ccGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCACACCCACACCCACACCCCCAAGGGGc                                                                                                                       >  1:3611294/1‑150 (MQ=38)
                                 cgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGTATTTTACATCCCGTACGTTGGCCTGCCCCGAAACCTATAGCCAAAgggggg                                                                                                                      >  2:3973502/1‑148 (MQ=37)
                                 cgcgCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCGCCGCACCCAAAACCACACCCCCAAGGGGCg                                                                                                                      >  2:711870/1‑150 (MQ=38)
                                  gcgcCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACAACCAAACCCACACCCCAAAAGGGCGa                                                                                                                     >  2:810622/1‑150 (MQ=39)
                                     ccAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAggg                                                                                                                  <  1:24081/150‑1 (MQ=255)
                                           gCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGt                                                                                                            >  2:3412647/1‑150 (MQ=255)
                                            cATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAACGGGGCGAGGGGACCGtt                                                                                                           <  1:3333759/150‑1 (MQ=255)
                                             aTCACCTTAACTATTGATAATAAAAGACATTAACCGTCGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCGAACCCCCACCCCCAAGGGGCGGGGGGGCCgggc                                                                                                          >  1:2032811/1‑147 (MQ=38)
                                             aTCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACCGTTc                                                                                                          >  2:3727100/1‑150 (MQ=255)
                                              tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTTAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:145928/150‑1 (MQ=255)
                                              tCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCa                                                                                                         <  1:3835760/150‑1 (MQ=255)
                                                 ccTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAAACCACTCCCCAAAGGGGCGAGGGGGCCGGGCAGTa                                                                                                      >  1:1749167/1‑150 (MQ=38)
                                                          ttGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCACCCAAACCCACAACCCAAAAGGGCGAGGGGGCCCGTCAGTACGGAAATTa                                                                                             >  1:2889799/1‑150 (MQ=37)
                                                           tGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                            <  1:772185/150‑1 (MQ=255)
                                                           cgATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAg                                                                                            <  1:772105/149‑1 (MQ=255)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCAAAACAACACCCCCAACGGGCGCGGGGACCGGTCAGTAAGGAATTTatg                                                                                           >  2:1569729/1‑148 (MQ=37)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                           <  2:189555/150‑1 (MQ=255)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGc                                                                                           <  2:198600/150‑1 (MQ=255)
                                                            gATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCACACCCCAAAGGGGCGTGGGGACCCGTCAGTACCGAAATTTGc                                                                                           >  2:1692926/1‑150 (MQ=38)
                                                             atcttaaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                          <  1:3689005/146‑1 (MQ=255)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCTCACCCAAAACCACAACCCAAAGGGGGGAGGGGGCCGGTAAGTTCGGAAATTAgga                                                                                          >  1:4021761/1‑148 (MQ=37)
                                                             ataataAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCt                                                                                          <  2:2204797/150‑1 (MQ=255)
                                                              taataaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCACACCCGAACCCACACCCCAAAGGGGCGCGGGGACCCGTCAGTTCGGAAGTTAGcat                                                                                         >  2:3599689/1‑148 (MQ=37)
                                                                 taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGAc                                                                                      <  1:2234675/150‑1 (MQ=255)
                                                                 taaAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCGCACCCTAACCCTCTACCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGGATGAc                                                                                      >  2:4088829/1‑150 (MQ=39)
                                                                  aaaaGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCACACCCGCTCCCCCAAGGGGCGAGGGGCCCCTTCACTACGGAAGTTAGCTTGACg                                                                                     >  2:2876163/1‑150 (MQ=38)
                                                                     aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:1076270/150‑1 (MQ=255)
                                                                     aGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTg                                                                                  <  1:1829268/150‑1 (MQ=255)
                                                                          ttAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATc                                                                             <  1:674848/150‑1 (MQ=255)
                                                                           tAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGGTCAGTACGGAATTTAGCTTGACGGTGCTATcc                                                                            <  2:1972212/150‑1 (MQ=255)
                                                                            taCCGTAGGCAGGATAAGATGCGCCAGCATCGCATCAGGCTATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCTCACACTAACCCTCTCCACAAAGGGGCGAGGGGTCCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  2:758704/149‑1 (MQ=255)
                                                                            aaCCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  1:3965835/150‑1 (MQ=255)
                                                                            aaCCGTAGGCCGGATAAGATGCGCCAGCAACGCATCCGGCGATGCTGGCGCGATGAATATTACATCCCGTACCTTCCCCTCACCCTAACCCTCTCCCCAAACGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCa                                                                           <  2:2510098/150‑1 (MQ=39)
                                                                              ccGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCCACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGc                                                                         <  2:243256/150‑1 (MQ=255)
                                                                               cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCa                                                                        <  1:2690369/150‑1 (MQ=255)
                                                                               cGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTACCCACACCCAAAACCACTACCCAAAGGGGCGCGGGGGCCGTTCAGTACGGAATTTAGGTTGACCGTGCTATACAGCa                                                                        >  2:124407/1‑150 (MQ=37)
                                                                                 tAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCAtt                                                                      <  2:2925995/150‑1 (MQ=255)
                                                                                   ggCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaa                                                                    <  1:3367441/150‑1 (MQ=255)
                                                                                    gCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTaaa                                                                   <  1:2998960/150‑1 (MQ=255)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCACACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGGGGAGTACGGAAATTAGCTTGACGGGGCTATCCCGCAAAAAAAACTg                                                              >  2:2467320/1‑150 (MQ=38)
                                                                                         aTAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTg                                                              <  2:2772608/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCACAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  2:2371643/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  1:486625/150‑1 (MQ=255)
                                                                                          tAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTgg                                                             <  1:2818785/150‑1 (MQ=255)
                                                                                           aaGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCTTACGTTCCCCTCACCCTCACCCTCTCCCCAACGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGc                                                            <  1:2040115/150‑1 (MQ=255)
                                                                                               tGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATa                                                        <  2:2256245/150‑1 (MQ=255)
                                                                                                 cgcCAGCATCGCCGGATGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCACACCCCAAAGGGGCGAGGGGACCCGTCAGTACGGAAGTTAGCTTGACGGTGCGATCCAGCAGTAAATAATGGCaaaaat                                                      >  2:424608/1‑146 (MQ=25)
                                                                                                  gcCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTGCCCGCCCCCGAACCCGCTCCCCCCCGGGGGGGGGGGGCCGGGCCGTACGGAAATTAGCTTTGCGGTGGTTGCCCGCCTTAAAAAGTGGGCATActc                                                     >  2:1205037/1‑150 (MQ=255)
                                                                                                    cAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACtctc                                                   <  2:2321790/150‑1 (MQ=255)
                                                                                                      gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATGCAGCATTAAATACTGGCAATACTCTCAc                                                 <  1:2492220/150‑1 (MQ=255)
                                                                                                      gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                 <  1:3511144/150‑1 (MQ=255)
                                                                                                      gcatcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCAc                                                 <  2:3694228/150‑1 (MQ=255)
                                                                                                        atcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGc                                               <  1:1936001/150‑1 (MQ=255)
                                                                                                         tcgcatcCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCAAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTATCTTGAAGGTGCTATCCAGCATTAAATAATGGCAATACACACAAGCa                                              >  2:341682/1‑150 (MQ=38)
                                                                                                           gcatcCGGCGATGCTGGCGCGTAGACTGTTACCTCCCGTACGGTCCCCTCACCCTACCCCCCTCCCCCACCGGGCGAGGGGACCGTTCAGTCCGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCaaa                                            <  2:1158641/150‑1 (MQ=255)
                                                                                                                cGGCGATTCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:1291862/150‑1 (MQ=255)
                                                                                                                cGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCg                                       <  2:2620774/150‑1 (MQ=255)
                                                                                                                 ggCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACGGGCAATACTCTCACGCATAATCCgc                                      <  2:3213702/150‑1 (MQ=255)
                                                                                                                  gCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCgca                                     <  1:301341/150‑1 (MQ=255)
                                                                                                                       gCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCAcc                                <  1:921416/150‑1 (MQ=255)
                                                                                                                         tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  1:1796473/150‑1 (MQ=255)
                                                                                                                         tGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGt                              <  1:1589252/150‑1 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCACTACCCACAGGGGCGAGGGGACCGGTCAGTACGGAATTTATCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTAACCCAAAATACGCAGCACCGtct                            >  2:506003/1‑150 (MQ=255)
                                                                                                                           gcgcGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAAACCACTCCCCAAAGGGGCCACGGGACCGGTCAGTACCGAATTTAGCATGGCGGTGCTATCCACCAATAAAAACGGGCAAAACACACACCCAAAAAACGCAGCACCctct                            >  2:2155942/1‑150 (MQ=255)
                                                                                                                              cgTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCtt                         <  2:2133828/150‑1 (MQ=255)
                                                                                                                                    aTTTTACATCCCGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGc                   <  1:4072241/150‑1 (MQ=255)
                                                                                                                                     ttttACATCCCGTACGTTCCCCTCACCCTAACCCTCACCCCAAAGGGGCGAGGGGACCCTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAGCAATAAAAACAGGCAAAACTCACACCCAAAAACCCCACCACCGACTCTTCAcccca                  >  2:2208756/1‑147 (MQ=25)
                                                                                                                                       ttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAACATTAAAAACAGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCAGc                >  2:3466791/1‑150 (MQ=255)
                                                                                                                                       ttACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAAATTAGCTTGACGGTGCTATCCAACATTAAATACTGGCAATACAATAACGCAAAAACCGCAGCACCCTCACTACACCGCAGc                >  2:3466789/1‑150 (MQ=37)
                                                                                                                                         aCATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGa              <  2:2717755/150‑1 (MQ=255)
                                                                                                                                              ccGTACGGTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCgg         <  2:3449294/150‑1 (MQ=255)
                                                                                                                                               cGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCggg        >  2:3079054/1‑150 (MQ=255)
                                                                                                                                               cGTACGTTCCCCTCACCCTAAACCTCTCCACAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATACAGCAATAAAAACTGGCAATAATATCACGCAAAATACGCAGCACCGTCTCTTCACCGCTGCGATTCggg        >  2:138232/1‑150 (MQ=38)
                                                                                                                                               cGTACGTTCCCCTCACCCTAAACCACTACCCCAAGGGGCGAGGGGACCGTTCCGTACGGAAATTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATAATCTCACGCAAAATACGCCCCACCCTCTCTTCACCGCTGCGATTCtgg        >  2:2498038/1‑150 (MQ=255)
                                                                                                                                                  aCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTa     <  2:3731568/150‑1 (MQ=255)
                                                                                                                                                  aCGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTa     <  2:1063729/150‑1 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCAc                      >  1:3310309/1‑131 (MQ=255)
                                                                                                                                                    gTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAAt   >  2:2805273/1‑150 (MQ=255)
                                                                                                                                                     ttCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCCCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATg  <  1:1207562/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GACAAACCGGTGCACGTCTCCGAACTCTTCTGCCGCGCCAATCGCATCACCTTAACTATTGATAATAAAAGACATTAACCGTAGGCCGGATAAGATGCGCCAGCATCGCATCCGGCGATGCTGGCGCGTTGAATTTTACATCCCGTACGTTCCCCTCACCCTAACCCTCTCCCCAAAGGGGCGAGGGGACCGTTCAGTACGGAATTTAGCTTGACGGTGCTATCCAGCATTAAATACTGGCAATACTCTCACGCAAAATCCGCAGCACCGTCTCTTCACCGCTGCGATTCGGGTTAATG  >  NC_000913/3504706‑3505004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: