Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,721 A→C 8.8% intergenic (+72/+30) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,7210AC 8.8% 603.6 / 9.9 216intergenic (+72/+30)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base A (120/71);  new base C (2/23);  total (122/94)
Fisher's exact test for biased strand distribution p-value = 1.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 224 total aligned reads displayed.

TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGG  >  NC_000913/3608572‑3608869
                                                                                                                                                     |                                                                                                                                                    
tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                                                      >  1:1579968/1‑150 (MQ=255)
 ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                                                     >  2:1079150/1‑150 (MQ=255)
 ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                                                     >  1:3933448/1‑150 (MQ=255)
    tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATACGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                                                  <  2:742717/150‑1 (MQ=255)
      gcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcg                                                                                                                                                >  1:1403999/1‑150 (MQ=255)
          tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                                                                            >  2:2977341/1‑150 (MQ=255)
          tGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCAt                                                                                                                                            >  1:1769085/1‑150 (MQ=255)
            cTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAAGGCGGCATCg                                                                                                                                          >  2:1951505/1‑150 (MQ=255)
             tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                                                         >  2:2420142/1‑150 (MQ=255)
              ggCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAATGGCGGCATCGaa                                                                                                                                        <  2:2740263/150‑1 (MQ=255)
              gccaaaTACGGGGGCGACGGTGCTGGTCACAGCTAATGCTTTAATATTTTTGCCAAGCACAAAAGCAAAACCCAACGAAAAATTCACCCCTATCGGTCCCCACCCCCCTCTGGCTACTGGTTTAGGCTCAGGCCAAAAGGCGGCATCGaa                                                                                                                                        <  2:865427/145‑1 (MQ=255)
               gCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAg                                                                                                                                       >  1:2815728/1‑150 (MQ=255)
                aaGATAGGGGGGGGAGGTTGCTGGTGACAGCGAATGCTTTATTTTTGTTGCGCAGTTGATAAGGCAAACCTATCGCAACATTGTGCGCGATGGGTCCCCTCGCCCCTCTGGGTAGTGGTTTTGGGTGAGGGGAAAAGGCGGCATCGAAGc                                                                                                                                      <  2:2038410/149‑1 (MQ=255)
                 aGATAGGGGGGCGAGGGTGCTGGTGACCGGGAATGATTTAATATTGTTGCGCAGGAGATAAGGCAACCCGATCGCAACATTGAGCGCTATCGGTCCCCTCGCCCCTCTGGGGAGTGGTTTTGGGTGAGGGGAAAAGGCGGCATCGAAGcc                                                                                                                                     <  2:2937826/150‑1 (MQ=37)
                      cGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCa                                                                                                                                >  2:3507490/1‑150 (MQ=255)
                        ggggCGTGGGTGGTGGTTACCGCGATTGCGTTAAGTTTGTTGCGCAGTAGTTACGGCAACCCTCCCGCAACTTTGCGCGCGATGGGGCCCCGCGCCCCTCTGGGTTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGc                                                                                                                              <  2:2881749/150‑1 (MQ=255)
                           gCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGcccc                                                                                                                           >  1:1955400/1‑150 (MQ=255)
                                 gTGCTGGTGACCGCTATTGGGTTATGTTTGTTGCGCTGGAGATACGGCAAACGGATCGCAACATTGAGCGCTATCGGTCCCCTCGCCCCTCTGGGTAGTGGGTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCaa                                                                                                                     <  2:161946/150‑1 (MQ=255)
                                  tGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCAGTCCCCTCGACCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                                                    >  2:3147158/1‑150 (MQ=255)
                                        tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGccgt                                                                                                              >  1:1104587/1‑148 (MQ=255)
                                        tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                                              >  2:3519702/1‑150 (MQ=255)
                                        tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                                              >  1:1637537/1‑150 (MQ=255)
                                          aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                                            >  1:2291215/1‑150 (MQ=255)
                                           ctgCGAATGCGTTAAGATTGTTGCGCAGTAGATAAGGCAAACCGATCGCAACTTTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTAc                                                                                                           <  2:2695959/148‑1 (MQ=255)
                                            aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACg                                                                                                          >  2:2001904/1‑150 (MQ=255)
                                             gCGATTGCTTTAAGTTTGTTGCGCAGGAGATACGGCAACCCGATCGCAACTTTGCGCGCGATCGGTCCCCTCGCCCCTGTGGGGAGTGGTTTCGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                                                         <  2:906029/150‑1 (MQ=255)
                                               gAATGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAAACCGTTCGAAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGTGGGTTTGGTTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                                                       <  2:2535810/150‑1 (MQ=255)
                                               gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCACCATTGAGCGCGATCGGTCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                                                      <  2:2324255/150‑1 (MQ=255)
                                               gAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                                                       >  2:336333/1‑150 (MQ=255)
                                                attGCGTTATGTTTGTTGCGCTGGATTTATGCAAAACCGATCTAAACTTTGGGCGCTATGGGTCCCCTCGCCCCTCTGGGTTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                                                      <  1:4040545/148‑1 (MQ=255)
                                                actGCTTTAAGTTTGTTGCGCTGGAGTTAGGGAAACCCGATCGAAAATTTGCGGGCGATCGGTCCCCGCGCCCCTGTGGGGATTGGGTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                                                      <  2:458185/148‑1 (MQ=255)
                                                aaTGCTTTAAGTTTGTTGCGCAGGAGATAAGGCAAAACGATCGCAAAATTGCGCGCTTTCGGTCCCCACGCCCCTCTGGGTATAGGTTTAGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                                                      <  2:609233/150‑1 (MQ=255)
                                                aaTGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATc                                                                                                      <  2:2070151/150‑1 (MQ=255)
                                                     gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                                                 >  2:1659941/1‑150 (MQ=255)
                                                      ttAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATaa                                                                                                >  1:3599283/1‑150 (MQ=255)
                                                         aGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGa                                                                                             >  1:3057593/1‑150 (MQ=255)
                                                           aTTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATa                                                                                           >  2:47494/1‑150 (MQ=255)
                                                             tgttgGGCTGTATATAAGGAAAACCTATCGAAACTTTGTGCGGGAGGGGTCCCCTCGCCCGTGTGGTTTGTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                                         <  1:3298426/150‑1 (MQ=255)
                                                             tgttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAg                                                                                         >  1:178152/1‑150 (MQ=255)
                                                              ttggcgcTGGAGTTTAGGAAAACCGATCGCAACATTGAGCGCTATCGGACCCCTCGCCCCTGTGGGTTGTGGTTTTGGGTGAGGTCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                                                        <  1:1962143/147‑1 (MQ=255)
                                                              gttgCGCTGGAGATACGGCAACCCGATCGAATATTTGCGCGCTATGGGTCCCCCCCCCCCTGGGGGGTGTGGGTTGGGGTGGGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                                                        <  1:364613/150‑1 (MQ=255)
                                                                   gcAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTc                                                                                   >  1:268786/1‑150 (MQ=255)
                                                                    cAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTcc                                                                                  >  1:2513222/1‑150 (MQ=255)
                                                                       gagaTACGACAACCCGATCGACACTTTGCGCGCTCTCGGCCCCCCGGCCCCTGGGGGTAGGGGGTTTGGGTGGGGGGCCAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATc                                                                               <  2:1964238/150‑1 (MQ=255)
                                                                       gagaTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATc                                                                               >  1:2832205/1‑150 (MQ=255)
                                                                          aTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                                                            >  1:4126239/1‑150 (MQ=255)
                                                                              gccAAACCGATCGCAACATTGCGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGGGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTctct                                                                        <  1:711371/148‑1 (MQ=255)
                                                                               gCAAACCGATCGCAACCTTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  2:3466216/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGCATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  2:2217474/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  2:284335/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  2:1421224/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  1:2291992/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  1:2604218/1‑150 (MQ=255)
                                                                               gCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCtt                                                                       >  1:303053/1‑150 (MQ=255)
                                                                                   aCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGc                                                                   >  1:1916963/1‑150 (MQ=255)
                                                                                    cgtttCGCATCATTGAGCGGGATGGGTCCCGTCCCCGCTCTGGGGAGAGGGTTTGGGGGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                                                  <  2:3253146/146‑1 (MQ=255)
                                                                                    ccGATCGCAACAGTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCACTCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                                                  >  1:3923993/1‑150 (MQ=255)
                                                                                    ccGAACGCAAAATTGCGCGCGATGGGTCCCCGCGCCCCTCTGGGGTGAGGGTTAGGGTGTGGGGAAACGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCa                                                                  <  1:601957/150‑1 (MQ=255)
                                                                                      gaccGCAACTTTGAGCGCTCGCGGCCCCCCCCCCCCTGTGGGGTGTGGGTTTGGGTGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                                                <  1:3840975/147‑1 (MQ=255)
                                                                                           cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                                           >  1:2055988/1‑150 (MQ=255)
                                                                                            aaCTTTGTGCGCCAGCGGCCCCCCCGCCCCTCTGGGTCGTGGGTTTGGGTGAGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTc                                                          <  2:21619/150‑1 (MQ=255)
                                                                                             aCATGGCGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGTGGGTTAGGGTGAGGGGAACAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCg                                                         <  1:3437046/150‑1 (MQ=39)
                                                                                                gtgcgcgcGATCGGCCCCCCCGCCCCTGTGGGGTGTGGGTTTGGGTGTGGCCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCgtgt                                                      <  1:1082554/146‑1 (MQ=255)
                                                                                                    gcgcGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCa                                                  >  1:327452/1‑150 (MQ=255)
                                                                                                        gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                                              >  1:869976/1‑150 (MQ=255)
                                                                                                        gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                                              >  1:3632603/1‑150 (MQ=255)
                                                                                                         cgcgtgccccccccccCGCGGGGGCGTGGGTGCGGGTGAGGGTAAACGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                                             <  1:1416617/144‑1 (MQ=255)
                                                                                                             taacccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc                                         >  1:1897907/4‑150 (MQ=255)
                                                                                                              tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAATGGTACa                                        >  2:496931/1‑150 (MQ=255)
                                                                                                              tCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACa                                        >  2:1564062/1‑150 (MQ=255)
                                                                                                               ccccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaa                                       >  2:1568778/1‑150 (MQ=255)
                                                                                                                cccTCGCCCCTCGGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTTCaaa                                      <  2:4082711/150‑1 (MQ=255)
                                                                                                                 ccTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTGACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCACCCAGTTCGTGTTCCATAAAGGTACaaaa                                     >  2:3796655/1‑150 (MQ=255)
                                                                                                                    cGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                 <  2:3190815/150‑1 (MQ=255)
                                                                                                                     gCCCCTGTGGGTAGCGGGTTTGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                 <  2:2908129/150‑1 (MQ=255)
                                                                                                                     cccccccTGGGGAGAGGGTAAGGGTCAGGGTAAAAGGCGGCATCGAAGCCAAACAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCa                                 <  2:3664693/149‑1 (MQ=255)
                                                                                                                       cccTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAgg                               >  2:3532193/1‑150 (MQ=255)
                                                                                                                         ctctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCAATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGaa                             >  2:1622541/1‑150 (MQ=255)
                                                                                                                          tggggggTGTGGTTTTGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat                            <  2:1091999/147‑1 (MQ=255)
                                                                                                                            tGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAatat                          >  2:132728/1‑150 (MQ=255)
                                                                                                                            tGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAatat                          >  1:1153456/1‑150 (MQ=255)
                                                                                                                             ggggAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATc                         >  2:2749339/1‑150 (MQ=255)
                                                                                                                              gggAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATcg                        >  1:3160969/1‑150 (MQ=255)
                                                                                                                                gagtGGGTTAGGTTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATcgcg                      <  2:2408679/150‑1 (MQ=255)
                                                                                                                                gagaGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATcgcg                      >  1:100886/1‑150 (MQ=255)
                                                                                                                                 tgtgggTTCGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCgg                     <  2:780228/147‑1 (MQ=255)
                                                                                                                                    gggTTCGGGTGCGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTa                  <  1:2929733/150‑1 (MQ=255)
                                                                                                                                     ggTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAg                 >  2:2103237/1‑150 (MQ=255)
                                                                                                                                      gTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGt                >  2:157516/1‑150 (MQ=255)
                                                                                                                                       tttgggTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTg               <  1:3507490/147‑1 (MQ=255)
                                                                                                                                          gggggcggggACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGcc            <  1:1423679/144‑1 (MQ=255)
                                                                                                                                          gggTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGACTCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGcc            <  2:2783875/150‑1 (MQ=255)
                                                                                                                                              gAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAt        <  1:2030737/150‑1 (MQ=255)
                                                                                                                                               aGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtc       <  1:1659941/150‑1 (MQ=255)
                                                                                                                                                 gggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgt     >  1:1075124/1‑150 (MQ=255)
                                                                                                                                                 gggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgt     <  2:1718857/150‑1 (MQ=255)
                                                                                                                                                 gggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgt     <  1:666635/150‑1 (MQ=255)
                                                                                                                                                  ggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgtc    <  2:4126239/150‑1 (MQ=255)
                                                                                                                                                  ggAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGAtcgtc    >  2:1450248/1‑150 (MQ=255)
                                                                                                                                                    aaaaGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCgg  >  2:1024051/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAATATCGCGGGTAGTGGCCGGATCGTCGG  >  NC_000913/3608572‑3608869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: