Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,708,429 T→C 5.1% intergenic (‑724/+187) dppA ← / ← proK dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,708,4290TC 5.1% 790.4 / 21.2 238intergenic (‑724/+187)dppA/proKdipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base T (89/135);  new base C (3/9);  total (93/145)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 239 total aligned reads displayed.

ATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGA  >  NC_000913/3708281‑3708577
                                                                                                                                                    |                                                                                                                                                    
    ggCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                                                                                 >  1:106962/1‑150 (MQ=255)
    ggCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCa                                                                                                                                                 <  1:3353057/150‑1 (MQ=255)
     gCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAg                                                                                                                                                <  1:995213/150‑1 (MQ=255)
      cGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAgg                                                                                                                                               <  1:1446227/150‑1 (MQ=255)
           gcgcCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAATTGCCTGCTCGGTGAGCAGGCtttt                                                                                                                                          >  1:1286449/1‑150 (MQ=255)
           gcgcCGCATCCGACAATGGCCGGCACATTTGACTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAATAAGCCTGCTCGTTGAGCAGGCtgtg                                                                                                                                          >  1:1891286/1‑147 (MQ=255)
            cgccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTc                                                                                                                                         <  1:1349387/150‑1 (MQ=255)
            cgccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCAGCTCGTTGAGCAGGCTTTTc                                                                                                                                         >  1:432450/1‑150 (MQ=255)
             gccgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCGGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCACATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCtttttt                                                                                                                                        <  1:2707238/150‑3 (MQ=25)
               cgcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                                      <  1:1589556/150‑1 (MQ=255)
                gcATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCAACGAGCAGGCttttttgca                                                                                                                                     <  2:2695000/150‑6 (MQ=255)
                  aTCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                                   <  1:732924/150‑1 (MQ=255)
                  aTCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                                   <  1:2065624/150‑1 (MQ=255)
                  aTCCGACAAGGGCCGGCACATTGGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCGGCTCGTTGAGCAGGCTTTTCGAAttt                                                                                                                                   <  1:3181243/150‑1 (MQ=255)
                       ccactGGCCGGCACATGTGCCTGATGCGACGCTGCCGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTATCGAATTTGGTCg                                                                                                                              <  2:499583/146‑1 (MQ=255)
                       aCAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCg                                                                                                                              <  1:3450503/150‑1 (MQ=255)
                         aaTGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGt                                                                                                                            <  2:3558654/150‑1 (MQ=255)
                          aTGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                                      >  2:2167580/1‑139 (MQ=255)
                          aTGGCCGGCACATTTGCCTGATGCGACGCGGACGCGTCTTATCAGGCCTACCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGaa                                                                                                                                      <  1:2167580/139‑1 (MQ=255)
                             gCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATa                                                                                                                        <  2:551321/150‑1 (MQ=255)
                              ccagCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATag                                                                                                                       >  2:3503931/4‑150 (MQ=255)
                              ccGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTGTTCGAATTTGGTCGGTGATag                                                                                                                       >  1:2408716/1‑150 (MQ=255)
                              ccGGCACATGGGCCTGATGCGACGCTGACGCGTCTTATCCGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATag                                                                                                                       <  2:4019857/150‑1 (MQ=255)
                               cGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAAATTGGTCGGGGATaga                                                                                                                      >  1:679445/1‑150 (MQ=255)
                                ggCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATagag                                                                                                                     <  2:1935443/150‑1 (MQ=255)
                                  cacaTTTGCCTGATGCGCCGCTGACGCGTCTTATCAGGCCGACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                   <  2:1156273/150‑1 (MQ=255)
                                  cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                   <  2:2524096/150‑1 (MQ=255)
                                  cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                   <  1:3413224/150‑1 (MQ=255)
                                  cacaTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGa                                                                                                                   <  2:3016432/150‑1 (MQ=255)
                                     aTTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTa                                                                                                                >  2:3330907/1‑150 (MQ=255)
                                     aTTAGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGGTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGAttc                                                                                                                >  2:1725095/1‑149 (MQ=255)
                                       ttGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACt                                                                                                              <  2:2463838/150‑1 (MQ=255)
                                          ccTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGc                                                                                                           <  1:1573501/150‑1 (MQ=255)
                                                  gACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTg                                                                                                  >  1:3460846/1‑150 (MQ=255)
                                                   aCGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCACATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTg                                                                                                  <  1:1731567/150‑1 (MQ=255)
                                                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                                                                                                 <  2:3915918/150‑1 (MQ=255)
                                                    cGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTgg                                                                                                 <  1:3623202/150‑1 (MQ=255)
                                                     gCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGc                                                                                                >  1:607012/1‑150 (MQ=255)
                                                          cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                                                                           <  2:1324655/150‑1 (MQ=255)
                                                          cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                                                                           <  2:1884274/150‑1 (MQ=255)
                                                          cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                                                                           <  2:3539061/150‑1 (MQ=255)
                                                          cgcgTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCAGCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGcc                                                                                           <  2:3023481/150‑1 (MQ=255)
                                                             gTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGCGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTg                                                                                        <  1:2028054/150‑1 (MQ=255)
                                                              tCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGc                                                                                       >  1:1248270/1‑150 (MQ=255)
                                                                ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg                                                                                     <  1:1176730/150‑1 (MQ=255)
                                                                ttATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCgg                                                                                     <  1:1640357/150‑1 (MQ=255)
                                                                   tCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGcc                                                                                  <  2:2226184/150‑1 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGtt                                                                               >  2:1370553/1‑150 (MQ=255)
                                                                      ggCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGtt                                                                               <  1:408828/150‑1 (MQ=255)
                                                                       gCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTg                                                                              >  1:3796075/1‑150 (MQ=255)
                                                                       gCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCACCGTCGGGGCACGCCCGGCGGGCGGTTg                                                                              >  2:3186019/1‑150 (MQ=14)
                                                                             gtcgagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGGAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGgcg                                                                        <  2:2600540/150‑1 (MQ=255)
                                                                                gagtcgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCaa                                                                     <  1:3293245/150‑1 (MQ=255)
                                                                                    cgaACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGtt                                                                 <  2:1837965/150‑1 (MQ=255)
                                                                                      aaCCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGGTGCTGGCGCAACGGtct                                                               >  2:2386407/1‑150 (MQ=255)
                                                                                       aCCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATTCAAAAAAGCCTGCTCGTTGAGCAGGCTGTTCGAAATTGGTCGGTGATATAGGATTACACGCCACAACGTCGCTCGCCCTGCGGGCCGTTGCTGGCGCACCGAtctc                                                              >  1:1699461/1‑150 (MQ=17)
                                                                                        ccTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct                                                             <  1:919113/150‑1 (MQ=255)
                                                                                        ccTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct                                                             <  2:1277862/150‑1 (MQ=255)
                                                                                        ccTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTtctct                                                             <  2:3520732/150‑1 (MQ=255)
                                                                                          tCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcg                                                           <  1:333252/150‑1 (MQ=255)
                                                                                          tCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGGTCTCtcg                                                           >  1:584688/1‑150 (MQ=255)
                                                                                          tCCCTGCCCCCGGAGGTTCACATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCtcg                                                           <  1:2510195/150‑1 (MQ=255)
                                                                                             cTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGAGGCTCGCCCGGCGGCCCGTAGCTGGCGCAACGATCACtcgcta                                                        >  2:648811/1‑149 (MQ=18)
                                                                                                   ccGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGGTCTCTCGCTTCGCTCg                                                  >  1:1285620/1‑150 (MQ=255)
                                                                                                    cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                                 <  2:3909374/150‑1 (MQ=255)
                                                                                                    cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGa                                                 <  1:2286204/150‑1 (MQ=255)
                                                                                                    cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGGTCTCTCGCTTCGCTCGa                                                 >  1:1428045/1‑150 (MQ=255)
                                                                                                    cGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACATTCTCTCGCTTCGCTCGa                                                 <  2:2881070/150‑1 (MQ=255)
                                                                                                      gAGGTTCTCATCCTCATGAGCTGCAGATGCAAACAAGCCTGCTCGTTGAGCAGGCTTTTCGACTTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGt                                               <  2:342938/150‑1 (MQ=255)
                                                                                                      gAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGt                                               <  1:2122430/150‑1 (MQ=255)
                                                                                                         gTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGa                                            <  1:528564/150‑1 (MQ=255)
                                                                                                           tctcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAAc                                          <  1:2575380/150‑1 (MQ=255)
                                                                                                           tctcATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAAc                                          <  1:3925937/150‑1 (MQ=255)
                                                                                                                tCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTccc                                     >  2:3698478/1‑150 (MQ=255)
                                                                                                                 ccTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGTCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCt                                    <  1:1865890/150‑1 (MQ=255)
                                                                                                                 ccTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCt                                    <  1:1820599/150‑1 (MQ=255)
                                                                                                                     aTGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGccc                                >  1:3974347/1‑150 (MQ=255)
                                                                                                                           tGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                          <  1:2534962/150‑1 (MQ=255)
                                                                                                                           tGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAg                          >  1:231742/1‑150 (MQ=255)
                                                                                                                            gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAgg                         <  1:1156735/150‑1 (MQ=255)
                                                                                                                            gCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAgg                         >  1:3712872/1‑150 (MQ=255)
                                                                                                                             cAGATGCAAAAATGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGt                        >  2:743954/1‑150 (MQ=255)
                                                                                                                             cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGt                        >  2:370970/1‑150 (MQ=255)
                                                                                                                             cAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGt                        <  1:3303204/150‑1 (MQ=255)
                                                                                                                               gATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTtc                      <  2:1806356/150‑1 (MQ=255)
                                                                                                                                     aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATcc                <  1:793754/150‑1 (MQ=255)
                                                                                                                                     aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATcc                <  1:3584175/150‑1 (MQ=255)
                                                                                                                                     aaaaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATcc                >  1:1485206/1‑150 (MQ=255)
                                                                                                                                      aaaaGCCTGCTCAACGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCACCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCt               >  2:2285772/1‑150 (MQ=255)
                                                                                                                                      aaaaGCCCGCTCGTTGACCAGGCTAATCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTTCTGTCTGAAAGTTCTCTAGCGTCGCTCGAGTCGTACCTCCCTGCCCCCGGAGGTTCTCATCCt               <  1:2814734/150‑1 (MQ=37)
                                                                                                                                       aaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTc              <  2:432450/150‑1 (MQ=255)
                                                                                                                                       aaaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTc              <  1:1367934/150‑1 (MQ=255)
                                                                                                                                        aaGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCa             >  2:3978742/1‑150 (MQ=255)
                                                                                                                                           ccTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGAGGCTCGCCCTGCGGGACTTTGCTGGCGCAACGTTCTCTCGCTACGCTCCAGTCGAAACACACTGCCCCAGCAGCTTCAAATCCTCAAGa          >  2:462635/1‑150 (MQ=25)
                                                                                                                                           ccTGCTCGTTGAGCAGGCTTTTAGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCACCCTGCGGGCCGTTTCTGGCGCAACGTTCTCTAGCTTCGCTCGAGTCGAACCTCCCAGCCCCCGCAGGTTCTCATCCTCATGa          >  1:2848082/1‑150 (MQ=38)
                                                                                                                                            cTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGGTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAagag         >  1:1908580/1‑150 (MQ=255)
                                                                                                                                                 ggTTGAGGAGTCTTTTCGTATGTGGTCGGCGCTAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGGTGCAGGCGCATCGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCa    <  2:3466943/149‑1 (MQ=37)
                                                                                                                                                 cGTTGAGCAGGCTTTTCGTATTTGGTCGGTGCTAGAGGATTACTCGCCACTTCGGGGCTCGCCCTGCGGGCGGTTGCAGGCGCAACGTTCTCTCGCGTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCa    <  2:1595378/150‑1 (MQ=38)
                                                                                                                                                 cGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCa    <  1:2953142/150‑1 (MQ=255)
                                                                                                                                                   ttGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCAGGAGCTGCAGa  >  1:3849729/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
ATAAGGCGGTCGCGCCGCATCCGACAATGGCCGGCACATTTGCCTGATGCGACGCTGACGCGTCTTATCAGGCCTACGTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGATGCAAAAAAGCCTGCTCGTTGAGCAGGCTTTTCGAATTTGGTCGGTGATAGAGGATTACTCGCCACTTCGTGGCTCGCCCTGCGGGCCGTTGCTGGCGCAACGTTCTCTCGCTTCGCTCGAGTCGAACCTCCCTGCCCCCGGAGGTTCTCATCCTCATGAGCTGCAGA  >  NC_000913/3708281‑3708577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: