Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,781,088 T→A 8.5% intergenic (+71/‑127) lldD → / → trmL L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,0880TA 8.5% 444.1 / 3.0 147intergenic (+71/‑127)lldD/trmLL‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (51/72);  new base A (10/2);  total (72/75)
Fisher's exact test for biased strand distribution p-value = 6.45e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

GCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGA  >  NC_000913/3780939‑3781237
                                                                                                                                                      |                                                                                                                                                     
gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                                                                       <  1:4018183/150‑1 (MQ=255)
gCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCt                                                                                                                                                       <  2:2769744/150‑1 (MQ=255)
    gATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCAct                                                                                                                                                   <  1:1939069/150‑1 (MQ=255)
      ttCGCGGGTGCTGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGATCCCATGGCGAAAGGGATTGCGGCATAGTCGTTTGCGCACCCTCACCCTAACCCTCTCCGTCTGGGAGAGGGGACCGTTCTGCGCTGTATGTACTCCCTCActct                                                                                                                                                 <  1:2354932/150‑1 (MQ=255)
         gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAACCCTCTCCCACAGGGAGAGGGGACCGTTCGGCGCTGTTTGTACTACCGCACACTgta                                                                                                                                              >  1:1356678/1‑148 (MQ=255)
         gCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAACCACTACCGCAGGGCGAGGGGACCCTTAGGCGCTGGATGTAAACCCCCACACTGaa                                                                                                                                              >  1:2720857/1‑150 (MQ=255)
          cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                             <  2:1840342/150‑1 (MQ=255)
          cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                             <  1:727348/150‑1 (MQ=255)
          cTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGaaa                                                                                                                                             <  1:3374775/150‑1 (MQ=255)
           tGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCAAAAACAAAACCACAGGGAGTGGGGACCGGTCGGCGCTTTATTTACTACCGCACACTaaaaa                                                                                                                                            >  1:682227/1‑149 (MQ=255)
            ggTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACg                                                                                                                                           <  1:3062842/150‑1 (MQ=255)
             gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCCCCCGAAACCACCACCACACGGGGGGGGGGCCCGTCGGCGCGGGTTTTTATCCCCCACGCTGaaaaaa                                                                                                                                          >  1:24155/1‑147 (MQ=255)
             gTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCACACCCAAACCCACGACCACCGGGGGCGGGGGCCCGTCGGGGCGGTTTTTACAACCACACACAGAAACGa                                                                                                                                          >  1:3596820/1‑150 (MQ=255)
              tGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCGAAACCACTCCCACACGGGGAGGGGTCCCGTCGGCGCTGTATGTAAAACCACACACAGAAACGac                                                                                                                                         >  1:1670533/1‑150 (MQ=255)
                cAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCACACCCGAACCCAAACCCTAAGGGCGGGGGGGCCGGTCGGCCCTGGGTGGACTGCCACACACTGGAAccccac                                                                                                                                       >  1:542028/1‑145 (MQ=255)
                 aGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACAcc                                                                                                                                      <  2:2302457/150‑1 (MQ=255)
                 aGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACAcc                                                                                                                                      <  2:3697066/150‑1 (MQ=255)
                           tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                            <  2:1102258/150‑1 (MQ=255)
                           tAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCtttt                                                                                                                            <  1:3299337/150‑1 (MQ=255)
                             aaGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCACACCCACACCCACAGGGGGAGGGGCCCGGTCGGCGCGGTTTGTTCTCCCGCACGCAGAAACGCCACCGCCCACgttttt                                                                                                                          >  1:4059484/1‑150 (MQ=255)
                               gagTTGCCTGCGGCACTGGCTCCCTTGGCGAAAGGTAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                        <  1:3272822/150‑1 (MQ=255)
                               gagTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtc                                                                                                                        <  2:4130396/150‑1 (MQ=255)
                                agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGTAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                       <  1:3439230/150‑1 (MQ=255)
                                agTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtct                                                                                                                       <  1:2634185/150‑1 (MQ=255)
                                 gTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                                      <  1:81031/150‑1 (MQ=255)
                                 gTTGCCTGCGGCACTGGCGCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTtctc                                                                                                                      <  1:1477947/150‑1 (MQ=255)
                                     ccTGCGGCACTGGCTCCCATGGCGCAAGGGAATGCGGCATAGGCGTTGGCGCCCCCTCACCCTAACCCTCTCCCGCCGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                                  <  1:1567117/150‑1 (MQ=255)
                                     ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTc                                                                                                                  <  2:3789240/150‑1 (MQ=255)
                                     ccTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCCCACCCGAACCCACTACCAAAGGGGGAGGGGGCCGGTGGGCGCGGTTTGTACACCCCCCCACGGAAACGACCCCCCACACATTTTTTGTcccgc                                                                                                                  >  1:2740744/1‑148 (MQ=255)
                                      cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                                                 <  1:3862679/150‑1 (MQ=255)
                                      cTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCg                                                                                                                 <  1:2506417/150‑1 (MQ=255)
                                        gCGGCCGTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTGGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGTGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                                               <  1:1262346/150‑1 (MQ=255)
                                        gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                                               <  2:3951541/150‑1 (MQ=255)
                                        gCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCGCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGcc                                                                                                               <  2:1279819/150‑1 (MQ=255)
                                         cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                                              <  2:1445740/150‑1 (MQ=255)
                                         cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                                              <  2:2164615/150‑1 (MQ=255)
                                         cGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGccc                                                                                                              <  2:1445994/150‑1 (MQ=255)
                                               tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                        <  1:2033286/150‑1 (MQ=255)
                                               tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                        <  1:3047774/150‑1 (MQ=255)
                                               tGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCgg                                                                                                        <  1:1319276/150‑1 (MQ=255)
                                                ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                       <  1:2295052/150‑1 (MQ=255)
                                                ggCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCggg                                                                                                       <  2:2887721/150‑1 (MQ=255)
                                                 gCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCAAAACCACACCCCCAGGGCGACGGGGCCCTTACGCGCGGTATGTAATCCCCCACACTGAAAAGACCCCGCACTCATTTTTTCTCCCGCGCCCCGCCgggg                                                                                                      >  2:1992884/1‑150 (MQ=255)
                                                   tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTTCGCCCCCGCACCCAAAACCAATCCCCCAGGGGGATGGGGCCCGGTGGCGCTGGTTTTGATACCACACACTGTAACCACACCCCACAAATTTTTTTTAACTCGCCCCACCcggggg                                                                                                    >  2:70838/1‑148 (MQ=255)
                                                   tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGGGCCCCCACCCCCCAAACCACACCCAAAGGGCGAGGGGGCCGTGCCGCCCGGGATGTACTCCCGCAAACAGGAACCACACCCCCCACATTTTTTCTCCCACCCCCCCCCcgggcg                                                                                                    >  1:4042057/1‑148 (MQ=255)
                                                   tCCCATGGCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgag                                                                                                    <  1:3976881/150‑1 (MQ=255)
                                                    cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                   <  1:1802518/150‑1 (MQ=255)
                                                    cccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                   <  1:2501760/150‑1 (MQ=255)
                                                    cccAGGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGgaga                                                                                                   <  1:1802803/150‑1 (MQ=255)
                                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                  <  1:1016750/150‑1 (MQ=255)
                                                     ccATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGagag                                                                                                  <  1:1015518/150‑1 (MQ=255)
                                                      cATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAgg                                                                                                 <  1:980918/150‑1 (MQ=255)
                                                       aTGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAggg                                                                                                <  2:2260413/150‑1 (MQ=255)
                                                          gcgcacgggACTGCGGCAGAGTCGGGTGCGCCCCCTCACCCGACCCCTCGCCCTCAGGGAGAGGGGCCCGGTCGGCGCTGTATGTACTCCCGCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                             <  2:70086/144‑1 (MQ=255)
                                                          gCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCg                                                                                             <  2:482190/150‑1 (MQ=255)
                                                          gCGAAAGGGAATGCGGCATAGTCGTGTGCGCCCCCGCAACCAAAACCACACCCACAACGAGATGGGACCGGACGGCGCTGTATGTAATCCCACACACAGAAACGACAACGCACACATTTTTTAAACCTAGCCCCACCCGGGGGAGGGCCg                                                                                             >  2:4010818/1‑150 (MQ=255)
                                                             aaaGGGAATGCGGCATAGTCGTTTGCGCCCCCCCACCCCAAAACACACCCCCAGGGGGAGGGGTCCCGTGGGCGCGGGAATTACTACCACACACTGAAAACACACCCCCCACTTTTTTTATCCCGCCCCCCCCCCGGGGGGGGGGCgggg                                                                                          >  2:3316146/1‑150 (MQ=255)
                                                              aaGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGt                                                                                         <  2:132196/150‑1 (MQ=255)
                                                                gggAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGa                                                                                       <  2:3427316/150‑1 (MQ=255)
                                                                  gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                     <  2:4090141/150‑1 (MQ=255)
                                                                  gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                     <  2:794036/150‑1 (MQ=255)
                                                                  gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                     <  1:2965856/150‑1 (MQ=255)
                                                                  gAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAgg                                                                                     <  2:793911/150‑1 (MQ=255)
                                                                   aaTGCGGCATAGTCGTTTGCGCCCCCTCACCCGAAACCACTACCTAACGGAGTGGGGACCCTTAGGCGCGGTATGTACTACCTCACACTGAAAACACACCGCACACATTTTTTATACCTCGCCCCACCCGGGGGAGGGCCgggggggggg                                                                                    >  2:3088191/1‑150 (MQ=255)
                                                                        ggCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGaaaa                                                                               <  1:998672/150‑1 (MQ=255)
                                                                          cATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGCCACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAgg                                                                             <  2:2059960/150‑1 (MQ=255)
                                                                              gTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAAAGACACCGCACTCTTTTTTTCTCCCTCGCCCCACCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGc                                                                         >  2:2493785/1‑150 (MQ=255)
                                                                                 gTTTGCGCCCCCTCACCCTAACCCACTACCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACACTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACt                                                                      >  2:1959219/1‑150 (MQ=255)
                                                                                              cACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAAtttt                                                         >  2:1277910/1‑150 (MQ=255)
                                                                                                 ccTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTg                                                      >  1:2244608/1‑150 (MQ=255)
                                                                                                  cTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                     >  2:2609456/1‑150 (MQ=255)
                                                                                                   tAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCt                                                    >  1:1868089/1‑150 (MQ=255)
                                                                                                         tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATACTg                                              >  1:10782/1‑150 (MQ=255)
                                                                                                         tctcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTATCTGCTATCCTg                                              >  2:2398251/1‑150 (MQ=255)
                                                                                                         cctcCCCCCGGGTGTGGGGACCGTGGGGGTTTTTTTGAACCCCCACACTCTCAACCCACCCCCAAATCTTTTTTTCCCCCCCCCCCCGCGGGGGAGCGGGCGGGGGTGCGGGCACAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                              <  2:3434966/149‑1 (MQ=255)
                                                                                                           tcCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                            >  2:3933857/1‑150 (MQ=255)
                                                                                                               tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                        >  1:1728574/1‑150 (MQ=255)
                                                                                                               tCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTGTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAACAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                        >  2:2711293/1‑150 (MQ=255)
                                                                                                                 aGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCa                                      >  1:3220159/1‑150 (MQ=255)
                                                                                                                   ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                    >  1:3952482/1‑150 (MQ=255)
                                                                                                                   ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGACGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTGCTGCTATCCTGCCCCCGCACt                                    >  2:606647/1‑150 (MQ=255)
                                                                                                                   ggAGAGGGGACCGTTCGGCGCTGGATGTACTCCCTCACTCGGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGGGAGGGGAAAAGGCCGCACTGCTCCCCAATTGTCTGCTATCCTGCCCCCGCACt                                    >  2:2778024/1‑150 (MQ=255)
                                                                                                                    gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                   >  1:2855181/1‑150 (MQ=255)
                                                                                                                    gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTa                                   >  1:3897522/1‑150 (MQ=255)
                                                                                                                          gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                              >  2:2576224/1‑150 (MQ=255)
                                                                                                                          gggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAgggg                              >  1:3717714/1‑150 (MQ=255)
                                                                                                                            gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                            >  2:898581/1‑150 (MQ=255)
                                                                                                                             aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCa                           >  2:3728029/1‑150 (MQ=255)
                                                                                                                               cGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGt                         >  2:1309722/1‑150 (MQ=255)
                                                                                                                                 ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                       >  1:3916675/1‑150 (MQ=255)
                                                                                                                                 ttCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTAt                       >  1:3857900/1‑150 (MQ=255)
                                                                                                                                       gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                 >  1:659457/1‑150 (MQ=255)
                                                                                                                                       gcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                 >  1:3794206/1‑150 (MQ=255)
                                                                                                                                                  tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt      >  1:1508544/1‑150 (MQ=255)
                                                                                                                                                  tCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACttt      >  1:1489788/1‑150 (MQ=255)
                                                                                                                                                    ccTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc    >  1:2403222/1‑150 (MQ=255)
                                                                                                                                                     cTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg   >  2:1937670/1‑150 (MQ=255)
                                                                                                                                                      tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCGCTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa  >  1:1032327/1‑150 (MQ=255)
                                                                                                                                                      tCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa  >  2:1907133/1‑150 (MQ=255)
                                                                                                                                                      tCACTCTGAAACGACACCGCACGCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGCGAACAGGCCGCACTGCTCACCAATTTTCTGCTCTCCTGCCCCCGCCCTCAGGGGGCAGTATGCTCAACATCGTACTTTACGa  >  2:214262/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
GCAAGATTCGCTGGTGCAGGGGCTGGGTAAAGAGTTGCCTGCGGCACTGGCTCCCATGGCGAAAGGGAATGCGGCATAGTCGTTTGCGCCCCCTCACCCTAACCCTCTCCCTCAGGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTCTCCCTCGCCCCTCCGGGGAGAGGGCCGGGGTGAGGGGAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGA  >  NC_000913/3780939‑3781237

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: