Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,270,143 C→T 6.9% intergenic (+541/+73) yhaC → / ← rnpB pentapetide repeats‑related protein/RNase P, M1 RNA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,270,1430CT 6.9% 322.9 / 8.2 117intergenic (+541/+73)yhaC/rnpBpentapetide repeats‑related protein/RNase P, M1 RNA component
Reads supporting (aligned to +/- strand):  ref base C (72/34);  new base T (3/5);  total (75/42)
Fisher's exact test for biased strand distribution p-value = 1.20e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 122 total aligned reads displayed.

ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269997‑3270291
                                                                                                                                                  |                                                                                                                                                    
aCTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGAAAGTCATTAATAGTTATGCCCCGACAAAAGCAAAAAACCCCCGAAGCGGGTTTTTAAGTAAAACAGGTGAAAAAGACCCATAATCCGCGTTATTTTGGGTATAGcgtc                                                                                                                                                   >  1:2638673/1‑150 (MQ=11)
  tGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                 <  1:2863469/150‑1 (MQ=17)
    aCCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCAACTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGAgg                                                                                                                                               >  2:2704714/1‑150 (MQ=255)
     ccGATAATCCGCTTTCTTTTGGGTATTGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGa                                                                                                                                              <  1:1176792/150‑1 (MQ=255)
           aGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtca                                                                                                                                        <  1:194079/150‑1 (MQ=37)
                ctttctttTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGtaattcat                                                                                                                                   >  2:1176444/1‑150 (MQ=17)
                     ttttGGGTATAGTGTCGTGGACAGTCAATCAACTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTTCGTAAACCAGGTGAAACAGACCGATAAGCCGCGTTCTTTTGGGTATAGCGTCGTGGACAGtcattcaacatta                                                                                                                              >  1:1100813/1‑144 (MQ=11)
                                      tGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCaa                                                                                                             <  1:2094251/150‑1 (MQ=37)
                                          cAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTCGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCACAAAc                                                                                                         >  2:1843542/1‑150 (MQ=255)
                                             tcattcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCAGCCCCGCCAAAAGCAAAAACccg                                                                                                      >  1:295805/1‑150 (MQ=255)
                                              cattcatCTTTCTGCCCCTCCAAAAGCATAAACCCGCCGAAGCGGTTTTTTACGTAAAGCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCTTTCTTCTTTCTGCCCCTCCAATAGCAAAAACccgc                                                                                                     <  2:2242448/150‑1 (MQ=11)
                                                ttcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCACCAAAACCAAAAACccgccg                                                                                                   >  1:3202675/1‑150 (MQ=255)
                                                 tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATCAGCCGCGGTCTGTTGGGGATAGCGTCGTGGACAGGCAATCAAATTTCTGCCCCTCCAAAAGCAAAATCccgccgc                                                                                                  >  2:1597823/1‑149 (MQ=11)
                                                 tcatCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCCTTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGa                                                                                                  >  2:129443/1‑150 (MQ=37)
                                                   atCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAg                                                                                                >  2:424539/1‑150 (MQ=37)
                                                      tttCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCgg                                                                                             <  1:1348708/150‑1 (MQ=37)
                                                          tGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGttt                                                                                         >  2:2821550/1‑150 (MQ=37)
                                                           gCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGtgtt                                                                                        >  2:1882271/1‑150 (MQ=255)
                                                            ccccTCCAAAAGCAAAAACCCGCCGACGCGGTTTTTTACGTAAACCTGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATCGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGttttt                                                                                       <  1:1354662/150‑1 (MQ=11)
                                                             cccTCCAAAAGCAAAAACCCGCCGCAGCGTGTTTTTACGTAAACCAGGTGAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTa                                                                                      <  1:1408879/150‑1 (MQ=255)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAACATCAAAAACCCGCCGAAGCGGGTGTTGACGt                                                                                   >  2:2160556/1‑150 (MQ=255)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                   >  1:1128165/1‑150 (MQ=37)
                                                                tCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGt                                                                                   >  1:856341/1‑150 (MQ=37)
                                                                tCCAAAACCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCTGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTGGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgctt                                                                                   <  1:3086916/150‑5 (MQ=11)
                                                                acaaaaaGCTAAAACCCGCCGCAGCGGTTTTTTACGTAAACCAGGTCAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCTTCGGCGGGtttttgctt                                                                                   <  1:1775769/147‑5 (MQ=11)
                                                                  cAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATCAGCCGCTTTCTTTTGGGGATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACggac                                                                                 >  2:2167216/1‑147 (MQ=11)
                                                                   aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTaaa                                                                                >  2:554832/1‑150 (MQ=37)
                                                                   aaaaGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCGTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTGGTTACGGaaa                                                                                >  2:554782/1‑150 (MQ=11)
                                                                    acaGCAAACACCCGCCGGAGCGGGTTTTTACGTAAACCAGGTTAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAt                                                                               <  2:2420283/148‑1 (MQ=255)
                                                                      cgCAAACACCCGCCGAAGCGGGTTTTTACGTACACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCa                                                                             <  1:99695/149‑1 (MQ=255)
                                                                       gtaaaaaCCCGCTTCGGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                            >  2:363120/3‑150 (MQ=255)
                                                                       gcaaccaCCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGACACGGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                            <  2:549839/144‑1 (MQ=255)
                                                                       gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATAATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAg                                                                            >  2:1168253/1‑150 (MQ=255)
                                                                       gCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTGTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGGCAATCAg                                                                            >  1:972756/1‑150 (MQ=11)
                                                                       gCAAAAACACGCCGAAGCGCGATATTAAGTAAACCAGGTGAAACTGAACGATAATCCGCTAACATTTGGGTATAGCGTCGTTGAACGTCAAACAAATATATACACATAAAAAATAAAAAACCCGCCGAAGCGGGTTTTAACGTAAATCAg                                                                            >  2:1167382/1‑150 (MQ=2)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAATTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                           >  2:1670379/1‑150 (MQ=255)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                           >  2:186979/1‑150 (MQ=38)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAgg                                                                           >  1:243834/1‑150 (MQ=38)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGGACATCAgg                                                                           >  2:612572/1‑150 (MQ=255)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGGCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGCAGCGGGTTTTTACGTAAATCAgg                                                                           >  2:612539/1‑150 (MQ=255)
                                                                        cAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGACGCGGGTTTTTACGTAAATCAgg                                                                           >  1:243841/1‑150 (MQ=255)
                                                                             aCCCGCCGGAGCGGGTTAGGACGTACATCAGGTTAAACGGCCCGATAAGCCGCTTTCTTTTGGGTAGAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCCCAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAACTCAGGTGaaa                                                                      <  2:3159839/150‑1 (MQ=11)
                                                                             aCCCGCCGCATCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGAGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                      <  2:1637078/150‑1 (MQ=255)
                                                                             aCCCGCCGAAGCGGTTTTTTACGTAAACCAGGTTAAACTGACCGATACGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGaaa                                                                      <  2:698552/150‑1 (MQ=255)
                                                                                cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGTGTATAGCGTCGTGGAGAGTCATTCATGTTTCTTCCCCTCCAAAATCAAAAACCCGCCTAAGAGGGTTTTTAAGTAAATAAGGTGAAAATg                                                                   >  1:295789/1‑150 (MQ=11)
                                                                                cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAAcag                                                                   >  1:1197913/1‑148 (MQ=37)
                                                                                cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATATTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTg                                                                   >  1:1197908/1‑150 (MQ=37)
                                                                                cgccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAATCAAAAACCCGCAGAAGCGGGTTTTTTAGTAAATCAGGTGTAAATg                                                                   >  1:295931/1‑150 (MQ=11)
                                                                                 gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCCAAAGCAAAAACCCCCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                  >  1:276202/1‑150 (MQ=255)
                                                                                 gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCCAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACAGa                                                                  >  1:276209/1‑150 (MQ=255)
                                                                                 gccgAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCGCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGa                                                                  >  1:40233/1‑150 (MQ=37)
                                                                                     aaGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAATCATCTTTCAGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGGTTTTACGTAAATCAGGTGAAACTGACCGa                                                              >  2:1912980/1‑150 (MQ=255)
                                                                                       gCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATa                                                            >  2:299732/1‑150 (MQ=37)
                                                                                        cGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATaa                                                           >  2:1753673/1‑150 (MQ=37)
                                                                                           gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                        >  2:1244865/1‑150 (MQ=37)
                                                                                           gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                        >  1:626263/1‑150 (MQ=37)
                                                                                           gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                        >  2:1894806/1‑150 (MQ=37)
                                                                                           gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGcc                                                        >  1:3122628/1‑150 (MQ=37)
                                                                                           gTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCAGTCATCATTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGcc                                                        >  1:1684110/1‑150 (MQ=255)
                                                                                             ttttACGTACACCAGGTTACTCTGACCGATCAGCCGCTTTCTTTTGGGTATCGGGTGGTGGACAGTCATTCATCTTTCTGCCCCTCCACAAGCAAAAACCCGCCGTAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCgg                                                      <  1:1389798/150‑1 (MQ=11)
                                                                                               ttACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGt                                                    >  2:2673996/1‑150 (MQ=39)
                                                                                                       aCCAGGTGAAACTGACCGATAAGCCGCTTTCTTTGGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGt                                            <  2:3024346/150‑1 (MQ=255)
                                                                                                         cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                          >  2:457666/1‑150 (MQ=255)
                                                                                                         cAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTAGTGGAAAGTCAAACATCATTAAGCCCATCCAAAAGAAAAAAAACGCAGAAGCGGGTTTTTACCTAAATCAGGTTAAACTGACCGATAAGCCGGGTTCTGTCGTgg                                          >  2:457669/1‑150 (MQ=14)
                                                                                                          aGGTAAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGa                                         >  2:1342065/1‑150 (MQ=255)
                                                                                                             tGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGcgtcgt                                                                                                                                                 <  1:264956/43‑1 (MQ=38)
                                                                                                                   tGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATATTTATGCCCCACCAAAAGCAAAAACCCCCCCAAGCGGGTTTTTACGTAAATAATGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGGCAGtcattc                                >  1:2650547/1‑150 (MQ=17)
                                                                                                                    gACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACAGACCGATAAGCCGGGTTCTGTCGTGGACAgtcagtca                               >  2:1486154/1‑150 (MQ=255)
                                                                                                                     aCCGATCAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCATCAGTAAAAACCCGCCGAAGCGGGTTTTTACGTAAAACAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattcac                              <  1:1925611/150‑2 (MQ=14)
                                                                                                                     aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATACGCCGGGTTCTGTCGTGGACAGtcattcat                              <  1:1365487/150‑1 (MQ=255)
                                                                                                                     aCCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTAATCTTTCTGCCCCTCCAAAAGCAAAAAAACGCCGAAGCGGGTTTTTACGTAAATAAGGTGAAAATTTACGATAATCCGGGTTCTGTAGTGGACAGtcattaat                              >  2:2557445/1‑150 (MQ=17)
                                                                                                                       cGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCt                            <  2:2716328/150‑1 (MQ=255)
                                                                                                                        gATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTa                           <  1:402257/150‑1 (MQ=255)
                                                                                                                         aTAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAg                          <  2:671542/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:514568/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGTCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      >  2:1586841/1‑150 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      <  1:740172/150‑1 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      >  2:2145218/1‑150 (MQ=255)
                                                                                                                             gCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCa                      >  2:1570250/1‑150 (MQ=255)
                                                                                                                              ccGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAATCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:528682/150‑1 (MQ=255)
                                                                                                                              ccGCATTCTATTGGCTACAGCCTCGTGGCCAGTCATTCATCTTTCCGCCCCACCAACAGCAAAAACCCGCCGACGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTACTGTCGTGGACAGTCATTCATCTAGGCCAg                     <  1:2513764/150‑1 (MQ=18)
                                                                                                                               cGCTTTCTTTTGGGCATAGGGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGc                    <  1:2969024/150‑1 (MQ=255)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAATCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCa                   >  2:1010940/1‑150 (MQ=255)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAAACCCCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCCATAAGCCGGGTTCAGTCGTGGACAGTCATTCAACTAGGCCAGCa                   >  2:2992413/1‑150 (MQ=18)
                                                                                                                                gCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCACCAAAACCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAAAAGACCGATAAGCCGGGTTCTGTCGTGGACAGGCATTCATCTAGGCCAGCa                   >  1:294002/1‑150 (MQ=18)
                                                                                                                                 ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                  >  2:1267375/1‑150 (MQ=255)
                                                                                                                                 ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCaa                  >  2:2044338/1‑150 (MQ=255)
                                                                                                                                 ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATATAGGCCAGCaa                  >  2:1700290/1‑150 (MQ=255)
                                                                                                                                 ctttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTAAGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTATGCCAGCaa                  >  2:2044648/1‑150 (MQ=255)
                                                                                                                                  tttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAAt                 >  2:1092490/1‑150 (MQ=255)
                                                                                                                                   ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                <  2:3064232/150‑1 (MQ=255)
                                                                                                                                   ttctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATc                <  1:2821550/150‑1 (MQ=255)
                                                                                                                                    tctttTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCg               <  2:2638673/150‑1 (MQ=255)
                                                                                                                                       tttGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGtcattc                                >  2:686267/1‑130 (MQ=255)
                                                                                                                                         tGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCAc          <  2:672207/150‑1 (MQ=255)
                                                                                                                                            gTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTgg       <  2:2320407/150‑1 (MQ=255)
                                                                                                                                             tataGCGTCGTGGACAGTCAATCATCTTACTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCCGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGc      <  1:57454/150‑1 (MQ=255)
                                                                                                                                               taGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTc    <  1:2321010/150‑1 (MQ=255)
                                                                                                                                                 gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa  <  1:2471000/150‑1 (MQ=255)
                                                                                                                                                 gCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCaa  <  2:2445358/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
ACTGACCGATAAGCCGCTTTCTTTTGGGTATAGTGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAACCAGGTGAAACTGACCGATAAGCCGCTTTCTTTTGGGTATAGCGTCGTGGACAGTCATTCATCTTTCTGCCCCTCCAAAAGCAAAAACCCGCCGAAGCGGGTTTTTACGTAAATCAGGTGAAACTGACCGATAAGCCGGGTTCTGTCGTGGACAGTCATTCATCTAGGCCAGCAATCGCTCACTGGCTCAA  >  NC_000913/3269997‑3270291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: