Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,608,695 C→G 8.3% intergenic (+46/+56) zntA → / ← tusA zinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,608,6950CG 8.3% 478.8 / 9.2 144intergenic (+46/+56)zntA/tusAzinc, cobalt and lead efflux system/mnm(5)‑s(2)U34‑tRNA 2‑thiolation sulfurtransferase
Reads supporting (aligned to +/- strand):  ref base C (81/49);  new base G (2/12);  total (83/61)
Fisher's exact test for biased strand distribution p-value = 9.28e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 150 total aligned reads displayed.

CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAAT  >  NC_000913/3608547‑3608843
                                                                                                                                                     |                                                                                                                                                    
  cGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTgg                                                                                                                                                   <  2:68971/150‑1 (MQ=255)
   gCTGTTAGGGATGACCGGGTTGTGGCTGGCTGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  1:3092165/150‑1 (MQ=255)
   gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  1:2067478/150‑1 (MQ=255)
   gCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTggg                                                                                                                                                  <  2:1420584/150‑1 (MQ=255)
     tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGCTCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                <  2:1056196/150‑1 (MQ=255)
     tGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGga                                                                                                                                                <  2:2678722/150‑1 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                              <  1:2269460/150‑1 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                              <  2:2549411/150‑1 (MQ=255)
       ttAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGgaga                                                                                                                                              <  2:1706896/150‑1 (MQ=255)
        tAGGGCTGCCCGGGTTGGGGCTGGCAGTGCTGGCAGAGACGGGGGCGACGGTGCGGGTGACAGCGAATGCGTTAAGAGTGTTGCGCCGGAGAGACGGCAAACCGATCGCACCAGGGCGCGCGAGCGGTCCCCGCGCCCCTCTGGGGagag                                                                                                                                             <  2:2627407/150‑1 (MQ=255)
        tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGTGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             <  1:2330077/150‑1 (MQ=255)
        tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGTGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             <  1:2329973/150‑1 (MQ=255)
        tAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGagag                                                                                                                                             <  1:1971802/150‑1 (MQ=255)
               gACCGGGTGGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAgg                                                                                                                                      <  2:1726478/150‑1 (MQ=255)
                aCCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAggg                                                                                                                                     >  2:3062072/1‑150 (MQ=255)
                  cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                                                   >  1:1029787/1‑150 (MQ=255)
                  cGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTg                                                                                                                                   >  1:606207/1‑150 (MQ=255)
                      gtgtgGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGAGTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAggg                                                                                                                               <  1:642698/149‑1 (MQ=255)
                         tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                            >  1:1805995/1‑150 (MQ=255)
                         tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                            >  2:2614255/1‑150 (MQ=255)
                         tggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaa                                                                                                                            >  1:510540/1‑150 (MQ=255)
                          ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                           >  1:1532563/1‑150 (MQ=255)
                          ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                           >  1:2539870/1‑150 (MQ=255)
                          ggctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaa                                                                                                                           >  1:2539842/1‑150 (MQ=255)
                           gctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGaaaa                                                                                                                          >  1:1251159/1‑150 (MQ=255)
                            ctggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCACCATTGAGCGCGAGCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGACAAg                                                                                                                         >  2:2879306/1‑150 (MQ=255)
                             tggcAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGACTGCGGTAGGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAgg                                                                                                                        >  2:616128/1‑150 (MQ=255)
                              ggcAGTGGTGGCAGATTCGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCATCGGGGGATAGGGTTAGGGTGAGGTTAAAAggc                                                                                                                       <  2:2252765/150‑1 (MQ=255)
                                ctgTGCTGGCATTTAGGGGGGCGAGGGTGGTGGTTACTGCTATGTGTTTTTGTTTTTTGCGCTGGTGTTAAGGCAACCGTATCGAAACTTTTAGCGCGTTGGGTCCCCTCGCCCCTCTGGGGTGTGGGTTTGGGTGCGGGTAAAAggcgg                                                                                                                     <  2:91333/148‑1 (MQ=255)
                                cAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcgg                                                                                                                     >  2:1062862/1‑150 (MQ=255)
                                 aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                    >  2:869611/1‑150 (MQ=255)
                                 aGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAggcggc                                                                                                                    >  1:1584551/1‑150 (MQ=255)
                                  gTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCa                                                                                                                   >  2:1988702/1‑150 (MQ=255)
                                    gctggctgATACGGGGGCGACGGTGCTGGTGACCGCGATTGCGTTAATATTGTTGCGCAGGAGATATGGCAAACCGATCGCAACATTGAGCGCGTTGGGTCCCCTCGCCCCTCTGGGGATTGGGTTAGGGTGAGGGGAAAAGGCGGCATc                                                                                                                 <  2:763931/150‑1 (MQ=38)
                                      tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                               >  1:1416995/1‑150 (MQ=255)
                                      tGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGa                                                                                                               >  1:1416932/1‑150 (MQ=255)
                                         ctgttcggggggCGAGGGTGGTGGTGACAGCGAATGCGTTAAGTTTGTTGCGCAGGAGATAAGGCAAACCGATCGAAACATTGAGCGCGATCGCTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGTAAAAGGCGGCATCGAAGc                                                                                                            <  2:2045113/143‑1 (MQ=38)
                                               cGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCa                                                                                                      >  1:2709044/1‑150 (MQ=255)
                                               cGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCa                                                                                                      >  1:1886866/1‑150 (MQ=255)
                                                   ggCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTGGCGCAGGAGATAAGGCAAACCGATCGCAACATTGCGCGCGAGCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGccc                                                                                                  <  2:2397641/150‑1 (MQ=255)
                                                           tGCTGGTGACAGCCAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAAc                                                                                          >  2:1875487/1‑150 (MQ=255)
                                                                gTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCt                                                                                     >  1:2296119/1‑150 (MQ=255)
                                                                gTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCt                                                                                     >  1:1602147/1‑150 (MQ=255)
                                                                 tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                    >  2:3223532/1‑150 (MQ=255)
                                                                 tGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCtt                                                                                    >  1:2867088/1‑150 (MQ=255)
                                                                   aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGCTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                  >  2:1235727/1‑150 (MQ=255)
                                                                   aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGTCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                  >  1:1940613/1‑150 (MQ=255)
                                                                   aCAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTa                                                                                  >  2:1028162/1‑150 (MQ=255)
                                                                    cAGCGAATGCGTTAAGATGGTTGCGCCGGAGATACGGCAACCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGTGAGGGTTAGGGGGAGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTAc                                                                                 <  2:2714638/150‑1 (MQ=38)
                                                                     aGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTGAGGGTGAGGGGAAAAGGCGGCATCGAAGCCACTCAGCCCCTATCAACCGCCGTTACg                                                                                >  2:116576/1‑150 (MQ=255)
                                                                      gCGAAGGCGGTAGGATGGTTGCGCTGGAGATCCGGCCACCGGTGCGCTCCATGGCGCGCGCGGGGTCCCCGCGCCCCTGTGGGGAGTGGGTTGGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGa                                                                               <  2:2814003/150‑1 (MQ=255)
                                                                        gAAGGCGTTATGTTTGTGGCGCTGTACATAAGGCAACCCGAGCGAAACATTGCGCGCTATGGGGCCCCTCGCCCCTCTGGGGTGTGGGTTAGGGTGCGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAt                                                                             <  1:2713297/150‑1 (MQ=255)
                                                                            gCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCaaa                                                                         >  1:1187434/1‑150 (MQ=255)
                                                                             ggTTATGTTTGTTGCGCTGTTTATAAGGCAAACCGATCGAAACATTGCGCGCGATGGGTCCCCTCGCCCCTCTGGGTTTTGGTTTTGGGTGTGGGTAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAAt                                                                        <  1:2430769/149‑1 (MQ=255)
                                                                              gTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATa                                                                       >  2:432882/1‑150 (MQ=255)
                                                                                      ttgttgCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATaa                                                                >  1:1117447/1‑150 (MQ=255)
                                                                                        gttgCGCAGTAGATAAGGCAACCCTATCGCAACTTTGAGCGCGTTGGGTCCCCTCGCCCCTCTGGGTAGTGGGTTCGGGTGAGGGGACAAGGCGGCATCGAAGCCAACCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAgg                                                              <  2:1820445/150‑1 (MQ=255)
                                                                                            cgcAGGATATAAGGAAACCCGATCGCAACTTTGCGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGCGGGTTCGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGt                                                          <  2:760774/150‑1 (MQ=38)
                                                                                               aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCa                                                       >  1:2635193/1‑150 (MQ=255)
                                                                                               aGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCGGGGGAGAGGGGTAGGGTGAGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAAGCAAATAACGAGAAGGCCGTCCa                                                       >  2:125941/1‑150 (MQ=39)
                                                                                                    aTAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGt                                                  >  2:2797353/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                           >  1:1011761/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                           >  2:3011412/1‑150 (MQ=255)
                                                                                                           aaaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTa                                           >  2:3011480/1‑150 (MQ=255)
                                                                                                            aaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAg                                          >  1:1110445/1‑150 (MQ=255)
                                                                                                            aaCCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAg                                          >  1:2320277/1‑150 (MQ=255)
                                                                                                               cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                       >  2:572426/1‑150 (MQ=255)
                                                                                                               cGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCaa                                       >  2:1225312/1‑150 (MQ=255)
                                                                                                                taTCGAAACTTTGCGCGCGATCGGCCCCCGCGCCCCTGTGGGGAGTGGGTTTGGGGGTGGGTACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                      <  1:23323/149‑1 (MQ=255)
                                                                                                                gATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                      >  1:221176/1‑150 (MQ=255)
                                                                                                                gATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAAc                                      >  2:2905649/1‑150 (MQ=255)
                                                                                                                  tCGCAACTTTGAGCGCGATGGGGCCCCTCGCCCCTCTGGGTAGTGGTTTCGGGTGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCa                                    <  2:3066780/150‑1 (MQ=255)
                                                                                                                   cGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGGTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTGTACGAATCAAATAACGATAAGGCCGGCCATCCGTCTCTTTAGCACCCAg                                   >  2:2371775/1‑150 (MQ=39)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  1:2361917/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  1:730327/1‑150 (MQ=255)
                                                                                                                     cAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGtt                                 >  1:730335/1‑150 (MQ=255)
                                                                                                                      aaCATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTc                                >  1:3191785/1‑150 (MQ=255)
                                                                                                                           tGAGCGCGATGGGTCCCCTCGCCCCTCTGGGGTGTGGTTTTGGGTGTGGGGACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGtt                           <  2:1251159/150‑1 (MQ=255)
                                                                                                                             aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                         >  2:1919955/1‑150 (MQ=255)
                                                                                                                             aGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTcc                         >  2:1919847/1‑150 (MQ=255)
                                                                                                                                 cgctCGGTCCCCTCGCCCCTGTGGGGTGCGGTTTTGGGTGTGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa                     <  1:2480607/147‑1 (MQ=255)
                                                                                                                                 cgATCGGTCCCCGCGCCCCTGTGGGGATTGGGTTCGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa                     <  1:2355040/150‑1 (MQ=255)
                                                                                                                                 cgATCGGCCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGACTCAAATCACGATAAGGCAGTCCATCCTTCTCTTTAGCAACCAGTTCGTGTTCCATaa                     <  2:3191160/150‑1 (MQ=39)
                                                                                                                                 cgATCGGCCCCCCCGCCCCTGGGGGGTGCGGGTTTGGGGGTGGGGCCAAGGCGGCATCGAAGCCAATCAGCCCCTATCACCCGCCTTTACGAATCACATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaa                     <  2:2494945/150‑1 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCCGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                    >  1:2650227/1‑150 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATaaa                    >  2:1143994/1‑150 (MQ=255)
                                                                                                                                  gATCGGTCCCCTCGCCCCTCTGGGGAGAGGGGGAGGGGGAGGGGAAAGGGCGGCCTCGAAGCCGATCCGCCCCGATCAACCGCCTGTACGAATAAAATAACGATAAGGCAGGCCTTCCGTCTCTGTAGCAGCCAGTTCGGGGTcccgaac                    >  2:1143987/1‑145 (MQ=25)
                                                                                                                                   aTCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAg                   >  2:2904538/1‑150 (MQ=255)
                                                                                                                                    tCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAgg                  >  1:3108470/1‑150 (MQ=255)
                                                                                                                                       gcccccGCGCCCCTGTGGGGTGTGGGTTCGGGGGAGGGCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc               <  1:837523/148‑1 (MQ=255)
                                                                                                                                       gTCCCCGCGCCCGTGGGGGGTGTGGTTTAGGGGGCGGGCACAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTAc               <  1:837420/150‑1 (MQ=255)
                                                                                                                                          cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaa            >  1:2125519/1‑150 (MQ=255)
                                                                                                                                          cccTCTCCCCAGAGGGGCGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaa            >  2:3154979/1‑150 (MQ=255)
                                                                                                                                          cccTCGCCCCTCGGGGGGGAGGGGTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCCTCCGTCTCTTTAGCAACCAGTTCGTGTTCCAGAAAGGTACaaa            >  2:2951784/1‑150 (MQ=255)
                                                                                                                                           ccTCCCCCCTCTGGGTAGAGGTTTAGGGTGAGGCCAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACaaaa           <  2:1029787/150‑1 (MQ=255)
                                                                                                                                            cTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAAc          >  2:2063304/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGTGGGTTTGGGTGTGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat  <  1:613386/150‑1 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTTGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat  <  2:1639866/150‑1 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat  >  2:679818/1‑150 (MQ=255)
                                                                                                                                                    tctGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAat  >  2:1615852/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CACGCTGTTAGGGATGACCGGGTTGTGGCTGGCAGTGCTGGCAGATACGGGGGCGACGGTGCTGGTGACAGCGAATGCGTTAAGATTGTTGCGCAGGAGATAAGGCAAACCGATCGCAACATTGAGCGCGATCGGTCCCCTCGCCCCTCTGGGGAGAGGGTTAGGGTGAGGGGAAAAGGCGGCATCGAAGCCAATCAGCCCCTATCAACCGCCTTTACGAATCAAATAACGATAAGGCAGTCCATCCGTCTCTTTAGCAACCAGTTCGTGTTCCATAAAGGTACAAAACCCAGGAAT  >  NC_000913/3608547‑3608843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: