Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,153,447 A→G 5.9% intergenic (‑349/+249) ppc ← / ← argE phosphoenolpyruvate carboxylase/acetylornithine deacetylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,153,4470AG 5.9% 467.2 / 6.1 152intergenic (‑349/+249)ppc/argEphosphoenolpyruvate carboxylase/acetylornithine deacetylase
Reads supporting (aligned to +/- strand):  ref base A (54/85);  new base G (7/2);  total (63/89)
Fisher's exact test for biased strand distribution p-value = 3.30e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAG  >  NC_000913/4153301‑4153596
                                                                                                                                                  |                                                                                                                                                     
tAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGtt                                                                                                                                                    <  1:1847570/150‑1 (MQ=255)
  aCTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTgg                                                                                                                                                  <  1:2697629/150‑1 (MQ=255)
     tATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCa                                                                                                                                               >  1:1300284/1‑150 (MQ=255)
      aTGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAt                                                                                                                                              <  1:1443359/150‑1 (MQ=255)
      aTGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCAt                                                                                                                                              <  2:228298/150‑1 (MQ=255)
        ggAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg                                                                                                                                            <  2:190548/150‑1 (MQ=255)
        ggAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg                                                                                                                                            >  2:3006838/1‑150 (MQ=255)
        ggAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg                                                                                                                                            >  2:3006851/1‑150 (MQ=255)
        ggAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATACGGCCTACAGTTGGGCATCg                                                                                                                                            >  2:2093797/1‑150 (MQ=255)
         gAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGt                                                                                                                                           <  1:670052/150‑1 (MQ=255)
          aaaTGTTATAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGtt                                                                                                                                          <  2:1380950/150‑1 (MQ=255)
          aaaTGTTAAAAAATCGCCCCAAGTAACGCCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCTTCGtt                                                                                                                                          <  2:1380986/150‑1 (MQ=255)
          aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCGTCGtt                                                                                                                                          >  2:3080444/1‑150 (MQ=255)
          aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGtt                                                                                                                                          <  2:2158790/150‑1 (MQ=255)
          aaaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGtt                                                                                                                                          <  2:2159078/150‑1 (MQ=255)
           aaTGTTAAAAAATCGCCCCAAGTAACACCTAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                                                         <  2:3038726/150‑1 (MQ=255)
           aaTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGttt                                                                                                                                         <  2:1028299/150‑1 (MQ=255)
            aTGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTg                                                                                                                                        <  1:272455/150‑1 (MQ=255)
             tGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGa                                                                                                                                       <  2:2636830/150‑1 (MQ=255)
                 aaaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCa                                                                                                                                   <  2:1675037/150‑1 (MQ=255)
                 aaaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCa                                                                                                                                   <  2:3142235/150‑1 (MQ=255)
                  aaaacTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                                                  <  2:1935441/150‑1 (MQ=255)
                  aaaaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCAc                                                                                                                                  <  2:1234922/150‑1 (MQ=255)
                    aaaTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGTTTGGGAAACGTGTGAGTCCCTg                                                                                                                                >  2:1597324/1‑150 (MQ=17)
                      aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg                                                                                                                                            >  1:927229/1‑136 (MQ=255)
                      aTCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCg                                                                                                                                            <  2:927229/136‑1 (MQ=255)
                         gCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGATGGGCATCGTTTGAGTCACTgtcggt                                                                                                                           >  1:2613572/1‑150 (MQ=255)
                          ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                                                          <  2:840688/150‑1 (MQ=255)
                          ccccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtc                                                                                                                          >  2:842129/1‑150 (MQ=255)
                           cccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTgtcggtcg                                                                                                                         <  1:3117931/150‑1 (MQ=255)
                            ccAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGGTTGAGTGACTGtcggtcgg                                                                                                                        >  1:402811/1‑150 (MQ=255)
                             cAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGa                                                                                                                       >  1:1653925/1‑150 (MQ=255)
                             cAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGa                                                                                                                       <  2:2648354/150‑1 (MQ=255)
                                gTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATaa                                                                                                                    <  2:2144741/150‑1 (MQ=255)
                                gTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATaa                                                                                                                    <  2:2853043/150‑1 (MQ=255)
                                gTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATaa                                                                                                                    >  2:2997140/1‑150 (MQ=255)
                                  aaCACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGa                                                                                                                  <  2:2406190/150‑1 (MQ=255)
                                  aaCACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGa                                                                                                                  <  1:3129590/150‑1 (MQ=255)
                                   acacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGAt                                                                                                                 <  1:1532624/150‑1 (MQ=255)
                                   acacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGAt                                                                                                                 <  1:1532590/150‑1 (MQ=255)
                                    cacCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATg                                                                                                                <  2:2469436/150‑1 (MQ=255)
                                     acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATaa                                                                                                                    >  1:430542/1‑145 (MQ=18)
                                     acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATaa                                                                                                                    <  2:430542/145‑1 (MQ=18)
                                     acCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGAATCGTGAGAGTCACTGTAGGTCGGGTAATATgc                                                                                                               >  1:2277553/1‑150 (MQ=11)
                                       cAACGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgcgc                                                                                                             <  1:1089135/150‑1 (MQ=255)
                                       cAAAGGTGTAGGTCGGATAAGATGCGCTAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATgcgc                                                                                                             >  1:61672/1‑150 (MQ=255)
                                       cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTCGTTCGGCTAAGATgcgc                                                                                                             >  1:527015/1‑150 (MQ=255)
                                       cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTAAGAGTCAGTGTCTGTCGGGGAAGATgcgc                                                                                                             >  1:884533/1‑150 (MQ=11)
                                       cAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGAGAAGATgcgc                                                                                                             >  1:61731/1‑150 (MQ=255)
                                                 ggTCTGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCa                                                                                                   <  1:1949120/150‑1 (MQ=37)
                                                 ggTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGATTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCa                                                                                                   <  1:1048884/150‑1 (MQ=37)
                                                  gTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGGTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGGATCGCAt                                                                                                  >  1:1541224/1‑150 (MQ=21)
                                                   tCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATc                                                                                                 <  2:2826555/150‑1 (MQ=37)
                                                    cGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATcc                                                                                                <  1:2042515/150‑1 (MQ=37)
                                                    cGGATAAGATGCGCAAGTAGCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATcc                                                                                                <  2:1925228/150‑1 (MQ=37)
                                                                gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg                                                                                    <  1:1806681/150‑1 (MQ=37)
                                                                gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg                                                                                    <  2:2991340/150‑1 (MQ=37)
                                                                gcAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTGCTCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg                                                                                    <  2:618492/150‑1 (MQ=255)
                                                                gcAAGTATCGCAGCCGACATTATTGCGGCACTGGAGGTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCg                                                                                    <  2:618528/150‑1 (MQ=255)
                                                                   aGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCa                                                                                 <  1:586812/150‑1 (MQ=37)
                                                                       tCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGAATCGCATCCGACATTATTGCGGCACTgg                                                                             >  2:2754196/1‑150 (MQ=21)
                                                                       tCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTAAGAGTAACTGTCTTTCGGATAAGATGCGAAAGAATCGCATCAGACAATAAAGCGGCACTgg                                                                             >  2:1491415/1‑150 (MQ=11)
                                                                        cGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                                                            <  1:714013/150‑1 (MQ=37)
                                                                        cGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGa                                                                            <  1:2253886/150‑1 (MQ=37)
                                                                             ccGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTg                                                                       <  1:209922/150‑1 (MQ=37)
                                                                             ccGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTg                                                                       <  1:3006851/150‑1 (MQ=37)
                                                                                     attTCGGCTCTGGATTTTGTCTACTGTTCATGTTTCGGCGAGAGCGCCTTATCAGTCATACAGTTGGGCATCGTCTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGt                                                               <  2:1195647/150‑1 (MQ=11)
                                                                                         cGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                                                           >  2:2596773/1‑150 (MQ=39)
                                                                                         cGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGACGGATAAGATGCGCTAGGATCGCAGCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCg                                                           >  2:1804732/1‑150 (MQ=255)
                                                                                         cGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGTTAAGATGCGCAAGTATCGCATCCGACATTAATGCGGCACTGGAGTTTGGCAACAGTGCCg                                                           >  2:1311244/1‑150 (MQ=255)
                                                                                           gCACTGGAGTGTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGa                                                         <  2:2129043/150‑1 (MQ=255)
                                                                                               tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                     >  1:2539177/1‑150 (MQ=39)
                                                                                               tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcg                                                     >  1:2597498/1‑150 (MQ=39)
                                                                                               tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTAGTGCGGCACTGGAGTTTGGCAACAGAGCCGGATgcg                                                     >  2:3173051/1‑150 (MQ=255)
                                                                                               tGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGAATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGGATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGAAgcg                                                     >  2:2506838/1‑150 (MQ=255)
                                                                                                tgAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATgcgg                                                    <  1:2192396/149‑1 (MQ=39)
                                                                                                 gAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATgcggc                                                   >  2:626702/1‑150 (MQ=37)
                                                                                                 gAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGAGCCGGATgcggc                                                   >  2:626598/1‑150 (MQ=37)
                                                                                                        gCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcg                                            <  1:3252757/150‑1 (MQ=39)
                                                                                                        gCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcg                                            >  1:2453013/1‑150 (MQ=39)
                                                                                                        gCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGCATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAgcg                                            >  1:2453014/1‑150 (MQ=21)
                                                                                                          aaCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCCTCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGcc                                          >  2:3186329/1‑150 (MQ=255)
                                                                                                           aCAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCt                                         <  1:449795/150‑1 (MQ=39)
                                                                                                            cAGTGACGGATGCGGCGCGAGCGCCTTATCCTGCCTACAGTTGGGCATAGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCtt                                        <  2:2613572/150‑1 (MQ=255)
                                                                                                                gCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATcc                                    <  2:1099238/150‑1 (MQ=39)
                                                                                                                 ccGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGGATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCg                                   >  2:1032743/1‑150 (MQ=37)
                                                                                                                       aaggcgCTCGCGCCGCATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTAc                             >  2:337871/3‑150 (MQ=11)
                                                                                                                          gcgcGAGCGCCTTATCCGGCCTACAGTTTTGCAGCGTATGAGTCACTGTCGGTCGGATAAGATGAGCACGAATCGCATCCGACATTATTGCGGCACTGGATTTTGGCAACAGTGCCGGAAGCGGCGCGAGCGCCTTATCCGGCCTAcctt                          >  1:3202378/1‑147 (MQ=11)
                                                                                                                          gcgcGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGt                          >  1:2301460/1‑150 (MQ=40)
                                                                                                                          gcgcGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGt                          >  2:1352999/1‑150 (MQ=40)
                                                                                                                          gcgcGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGt                          >  1:3202394/1‑150 (MQ=40)
                                                                                                                            gcgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTg                        <  1:1701328/150‑1 (MQ=255)
                                                                                                                            gcgAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTg                        <  1:590107/150‑1 (MQ=255)
                                                                                                                                gcgcCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCa                    >  1:2890309/1‑150 (MQ=255)
                                                                                                                                        tCCGGCCTACTGTTGGGCATCGTGTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGa            <  2:2075691/150‑1 (MQ=255)
                                                                                                                                        tCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGa            <  1:1603652/150‑1 (MQ=255)
                                                                                                                                            tccTAAAGTTGGGCATCTTTTGAGTCTCTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCa        <  2:2389211/149‑1 (MQ=255)
                                                                                                                                            gCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATTCGCAAGTATAGCAACCCACAATATTGCGGGACTGGAGTTTGGCAACAGTGCCCGATGCGGCGCGAGCGCCTTATCCGGCCAACGGTTGGGCATCGTTTGAGTCa        >  1:2061057/1‑150 (MQ=17)
                                                                                                                                                  aGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAg  >  1:1977344/1‑150 (MQ=255)
                                                                                                                                                  aGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAg  >  1:1346107/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
TAACTTATGGAAATGTTAAAAAATCGCCCCAAGTAACACCAAAGGTGTAGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACAGTTGGGCATCGTTTGAGTCACTGTCGGTCGGATAAGATGCGCAAGTATCGCATCCGACATTATTGCGGCACTGGAGTTTGGCAACAGTGCCGGATGCGGCGCGAGCGCCTTATCCGGCCTACGGTTGGGCATCGTTTGAGTCACTGTAG  >  NC_000913/4153301‑4153596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: