Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 758,459 G→A 5.3% intergenic (+54/‑247) sdhB → / → sucA succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913758,4590GA 5.3% 845.5 / 22.7 246intergenic (+54/‑247)sdhB/sucAsuccinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamine triphosphate‑binding
Reads supporting (aligned to +/- strand):  ref base G (116/115);  new base A (2/11);  total (118/128)
Fisher's exact test for biased strand distribution p-value = 2.00e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

Only 100 of 250 total aligned reads displayed.

TCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTAC  >  NC_000913/758314‑758607
                                                                                                                                                 |                                                                                                                                                    
 catGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATgcggcg                                                                                                                                                 >  2:565403/1‑150 (MQ=255)
      aCTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGTTAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAAc                                                                                                                                            >  2:619297/1‑150 (MQ=255)
        tGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGc                                                                                                                                          <  2:1824524/150‑1 (MQ=255)
           gTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCTAGTCGTTGTTGTTGCAACGTAATGCGTACACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                       <  2:2437927/150‑1 (MQ=255)
           gTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                       <  2:1800531/150‑1 (MQ=255)
           gTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                       >  2:166525/1‑150 (MQ=255)
           gTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                       >  2:1345979/1‑150 (MQ=255)
           gTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCtt                                                                                                                                       >  1:2257054/1‑150 (MQ=255)
             cAGTGTATGTCCGACGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                     <  1:1427658/150‑1 (MQ=255)
             cAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTAt                                                                                                                                     <  1:1433203/150‑1 (MQ=255)
                tgtATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCg                                                                                                                                  <  1:548512/150‑1 (MQ=255)
                 gtATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                 <  2:2586294/150‑1 (MQ=39)
                 gtATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATAAGGCGTTCACGCCGCATCCgg                                                                                                                                 <  2:3082638/150‑1 (MQ=39)
                   aTGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGcc                                                                                                                               >  1:3083727/1‑150 (MQ=255)
                      tCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGGCCGGATAAGGCGTTCACGCCGCATCCGGcacttg                                                                                                                           <  2:970940/150‑6 (MQ=38)
                      tCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGGCCGGATAAGGCGTTCACGCCGCATCCGGcactgg                                                                                                                           <  2:1855028/150‑6 (MQ=38)
                       ccGATGGGGCTGAACCCGACGCGCGCCATCGGCCATATAATGTGGTTGTTGTTGCAACGTAATGCTTAAACCGTAGGCGTGATAAGACGCGCATGCGTCGCATCTGGAAACCTGTGCGGGATATGGCGTTCACGCCGCATCCGGcactgg                                                                                                                           <  1:2356006/150‑6 (MQ=255)
                       ccGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa                                                                                                                           >  2:955055/1‑150 (MQ=255)
                        cGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACaa                                                                                                                          <  1:1416575/150‑1 (MQ=255)
                             gggCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAt                                                                                                                     >  2:308954/1‑150 (MQ=255)
                              ggCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAtt                                                                                                                    >  1:1596805/1‑150 (MQ=255)
                                cTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCATGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTAc                                                                                                                  >  1:545409/1‑150 (MQ=255)
                                 tGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTAcc                                                                                                                 >  1:1228157/1‑150 (MQ=255)
                                  gAACCCGACGCGCGCCCTCGGCCATATCAAGTCGAGGTTGTTGCAACGGAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAGTAccc                                                                                                                <  2:1502104/150‑1 (MQ=255)
                                   aaCCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCg                                                                                                               >  2:1279123/1‑150 (MQ=255)
                                      ccGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAg                                                                                                            >  1:1748942/1‑150 (MQ=255)
                                       cGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAgg                                                                                                           <  1:2103666/150‑1 (MQ=255)
                                            cgcgcCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAGTACCCGTAGGCCTGa                                                                                                      <  2:1118350/150‑1 (MQ=255)
                                             gcgcCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGAt                                                                                                     <  2:2615952/150‑1 (MQ=255)
                                               gcCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATaa                                                                                                   <  1:422203/150‑1 (MQ=255)
                                                      gCCATATCAAGTCGATGTTGTTGCAACGTAATTCGTAAAACGTAGGCCTGATAAGACGCGCCAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCGTATCCGGCCTACAAGTCAATACCCGTAGGCCTGATAagcgcagc                                                                                            >  1:2776211/1‑150 (MQ=37)
                                                      gCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAagcgcagc                                                                                            >  2:1829250/1‑150 (MQ=255)
                                                         atatCAAGTCGATGTTGTTTCAACGTAAATCGTAAACCCTAGGCCTGATAAGACCCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCAGATAAgcgcagcgca                                                                                         >  1:1341513/1‑150 (MQ=37)
                                                            tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCa                                                                                      >  2:2808056/1‑150 (MQ=255)
                                                             cAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAg                                                                                     >  2:712962/1‑150 (MQ=255)
                                                             cAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAg                                                                                     <  2:493441/150‑1 (MQ=255)
                                                             cAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAg                                                                                     <  2:1534975/150‑1 (MQ=255)
                                                              aaGTCGATGTTGTTGCAACGTAATGCGTAAAACGTAGGCCGGATAAGAAGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAgg                                                                                    >  1:987579/1‑150 (MQ=255)
                                                                 tCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGt                                                                                 >  2:2076805/1‑150 (MQ=255)
                                                                      gttgttGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACaa                                                                            >  1:2293251/1‑150 (MQ=255)
                                                                          ttgCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGaa                                                                        <  2:686984/150‑1 (MQ=255)
                                                                             cAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATg                                                                     <  1:589621/150‑1 (MQ=255)
                                                                             cAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATg                                                                     >  1:890130/1‑150 (MQ=255)
                                                                               aCGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCa                                                                   <  2:2702909/150‑1 (MQ=255)
                                                                               aCGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCa                                                                   <  1:2563799/150‑1 (MQ=255)
                                                                               aCGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCa                                                                   <  1:1996358/150‑1 (MQ=255)
                                                                               aCGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCa                                                                   >  1:1727328/1‑150 (MQ=255)
                                                                                cGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAg                                                                  <  1:3153522/150‑1 (MQ=255)
                                                                                cGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAg                                                                  <  1:955055/150‑1 (MQ=255)
                                                                                 gTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAgg                                                                 <  1:719285/150‑1 (MQ=255)
                                                                                 gTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAgg                                                                 >  2:1681714/1‑150 (MQ=255)
                                                                                        gTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACACAGAAATGCAGGAAATCtt                                                          >  1:1857998/1‑150 (MQ=255)
                                                                                         tAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCttt                                                         <  2:1596805/150‑1 (MQ=255)
                                                                                         tAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCttt                                                         >  2:1995871/1‑150 (MQ=255)
                                                                                         tAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCttt                                                         >  2:873455/1‑150 (MQ=255)
                                                                                          aaaCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTa                                                        >  1:2589260/1‑150 (MQ=255)
                                                                                           aaCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTaa                                                       <  2:2233887/150‑1 (MQ=255)
                                                                                           aaCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTaa                                                       >  1:1564161/1‑150 (MQ=255)
                                                                                           aaCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTaa                                                       <  2:243129/150‑1 (MQ=255)
                                                                                            aCCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTaaa                                                      >  1:2191316/1‑150 (MQ=255)
                                                                                              cGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTaaaaa                                                    <  1:82075/150‑1 (MQ=255)
                                                                                                   gCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGcc                                               >  1:1110811/1‑150 (MQ=255)
                                                                                                    ccTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGccc                                              >  2:420311/1‑150 (MQ=255)
                                                                                                      tGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCt                                            >  1:2644607/1‑150 (MQ=255)
                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGa                                          <  2:2496657/150‑1 (MQ=255)
                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGa                                          <  2:2415861/150‑1 (MQ=255)
                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGa                                          <  2:2776041/150‑1 (MQ=255)
                                                                                                        aTAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGa                                          <  2:1108574/150‑1 (MQ=255)
                                                                                                          aaGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGaca                                        <  2:2274480/150‑1 (MQ=255)
                                                                                                           aGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGacac                                       >  1:2975371/1‑150 (MQ=255)
                                                                                                            gACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGTAATCTATAAAAACTGCCCCTGACACt                                      >  1:746946/1‑150 (MQ=255)
                                                                                                            gACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACt                                      >  2:668541/1‑150 (MQ=255)
                                                                                                            gACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACt                                      >  2:1359447/1‑150 (MQ=255)
                                                                                                            gACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACt                                      >  2:2971865/1‑150 (MQ=255)
                                                                                                             aCGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTa                                     <  1:2808056/150‑1 (MQ=255)
                                                                                                                cgcAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGa                                  >  2:2293977/1‑150 (MQ=255)
                                                                                                                     gCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGACATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAAACTTTAAAAACTGCCCCTGACACTAAGACAGtt                             <  1:1915491/150‑1 (MQ=255)
                                                                                                                     gCGTCGCATCAGGCAACCAGGGCCGGATGCGGCGTGAACGCCTGATCCGGCCTACAAGTCATTACCCGGAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGtt                             <  2:1592/150‑1 (MQ=255)
                                                                                                                          gCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTaa                        <  1:968219/150‑1 (MQ=255)
                                                                                                                           cATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTaaa                       <  2:812823/150‑1 (MQ=255)
                                                                                                                           cATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTaaa                       >  1:452747/1‑150 (MQ=255)
                                                                                                                             tCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCTGCCTACAAGTCATTACCCGTAGGCCTGATATGCGCAGCGCATCAGGCGTTACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTTAAgg                     <  2:901557/150‑1 (MQ=255)
                                                                                                                              cAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGt                    >  1:1480912/1‑150 (MQ=255)
                                                                                                                               aGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCCTCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGtt                   >  1:1673308/1‑150 (MQ=255)
                                                                                                                                ggCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTc                  <  1:1974549/150‑1 (MQ=255)
                                                                                                                                ggCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTc                  <  1:1267557/150‑1 (MQ=255)
                                                                                                                                  cAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCt                >  2:1751774/1‑150 (MQ=255)
                                                                                                                                  cAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCAGACACTAAGACAGTTTTTAAAGGTTCCt                >  1:2848572/1‑150 (MQ=255)
                                                                                                                                  cAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTATAAAAACTGACCCAGACACTAAGACAGTTTTTAAAGGTTACt                >  2:2383049/1‑150 (MQ=255)
                                                                                                                                        gTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGa          <  2:452747/150‑1 (MQ=255)
                                                                                                                                        gTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGa          <  1:1380736/150‑1 (MQ=255)
                                                                                                                                          gCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGc        >  1:1499709/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  1:1352670/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  1:1160257/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  2:395499/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  1:75995/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  2:1182193/1‑150 (MQ=255)
                                                                                                                                           ccGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGcc       >  2:2091211/1‑150 (MQ=255)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTAc  <  1:1627495/150‑1 (MQ=255)
                                                                                                                                                tGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTAc  <  1:833475/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TCATGAACTGCGTCAGTGTATGTCCGAAGGGGCTGAACCCGACGCGCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCAAGCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGTAGGCCTGATAAGCGCAGCGCATCAGGCGTAACAAAGAAATGCAGGAAATCTTTAAAAACTGCCCCTGACACTAAGACAGTTTTTAAAGGTTCCTTCGCGAGCCACTAC  >  NC_000913/758314‑758607

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: