BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	781,269	C→T	7.8%	intergenic (+47/‑100)	lysY → / → lysZ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,269	0	C	T	7.8%	444.7 / 18.9	148	intergenic (+47/‑100)	lysY/lysZ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base C (74/57); new base T (5/6); total (85/63)
Fisher's exact test for biased strand distribution p-value = 5.38e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 154 total aligned reads displayed.

TCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTC  >  NC_000913/781122‑781416

tCGATCCCGTACTCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCa                                                                                                                                                   <  1:879968/150‑1 (MQ=255)

 cgctCCCGTCATCACCCACCACTCGTGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                                                                                                                                                  <  1:2125787/147‑1 (MQ=255)

   aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTATAGCAGTTGACGTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAAAGCAGGTGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                                                >  1:550942/1‑150 (MQ=14)

   aTCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCa                                                                                                                                                >  2:2327733/1‑150 (MQ=255)

      ccGTCATCACCCACCACTCGGATCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTTAAGGATAACGTTGCGACAGCAACg                                                                                                                                             >  2:730014/1‑150 (MQ=255)

         tcatcaCCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCCGCAACGGcc                                                                                                                                          >  1:3177220/1‑150 (MQ=255)

               cccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTTACTTTTAATCAATTTGTGGCCGGTTCGAATACAGCACGACCCACCACGTTTAACATCAAAATAAGATGTTAAGAGTGTAGGATAACGTTGCGCCACCAACCGccccgagg                                                                                                                                    >  1:1291403/1‑145 (MQ=14)

               cccaccACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAgg                                                                                                                                    <  2:1637331/150‑1 (MQ=255)

                                    tCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCt                                                                                                               >  1:2405405/1‑150 (MQ=255)

                                      aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                                                                                                                                                  >  2:938316/1‑113 (MQ=255)

                                      aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAg                                                                                                                                                  <  1:938316/113‑1 (MQ=255)

                                      aGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGc                                                                                                             >  2:1362393/1‑150 (MQ=255)

                                            tAGAGCAGTTGACTTTTAATCATTTGGTCGCTGTTTCGAATCCTGCACGACCCACATGTTTTAACATCAAAATCATATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGAcc                                                                                                       <  1:1721634/150‑1 (MQ=14)

                                               agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACccac                                                                                                    >  1:1280403/1‑150 (MQ=255)

                                               agCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACACAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCTGCACGACccac                                                                                                    >  1:1712568/1‑150 (MQ=255)

                                                   gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt                                                                                                >  2:284887/1‑150 (MQ=255)

                                                    ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGAAGGGCGAGAGAAGCTAGTCAAACACCACAACccaccacta                                                                                               >  1:702256/1‑147 (MQ=11)

                                                     tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAATCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATACTGCACGACCCACCAATCt                                                                                              >  2:2151313/1‑150 (MQ=255)

                                                        gttttAATCTTTTGGTCGGTGGTTCGAATCCTGCACGACCCACGTGTTTTAACATCAAAGTCTGTTGTTTTGCGTTATGGATACGGTTGCGCCTGCAACGGCCCGTAGGGCGTGCGAAGCGAGTCATCCTGCACGACCCTCCAATCTTaa                                                                                           <  1:2486896/149‑1 (MQ=14)

                                                         ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTaaa                                                                                          >  2:1492155/1‑150 (MQ=255)

                                                           ttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGa                                                                                        <  2:687313/150‑1 (MQ=255)

                                                             aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGATTTAACTTCAAACTCAGAAGTTAAGCGAGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGAGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTAAAAGAtt                                                                                      >  1:2561361/1‑150 (MQ=17)

                                                             aaTCAATTGGTCGCAGGTTCGAATCCTGCACGACACACCAGTTTTAACTTCAAACTCAGAAGATAAGCTTGAAGGAAAACTTTCCTACAGCAACGGCCCGAAGGCCGAGCGAATAGAGTCATCCATCACAACCCCCACATCACAAAGAta                                                                                      >  1:1777379/1‑149 (MQ=9)

                                                               tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGATGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTgg                                                                                    <  1:2442334/150‑1 (MQ=255)

                                                               tCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGAGAAGCGAGTCATCATGCACGACACACCAATCTAAAAGATTgg                                                                                    >  1:1320069/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                                  >  2:434652/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                                  >  2:434240/1‑150 (MQ=255)

                                                                 aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGcc                                                                                  >  2:2162627/1‑150 (MQ=255)

                                                                  tttGGTCGCTGGTTCGACTCCTGCACGACCCACCTGTTTTAACATCAAACTCAGATGTTAAGCGTGATGGATACCGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGccc                                                                                 <  1:2539629/149‑1 (MQ=17)

                                                                  aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACGTCAAACTCAGATGTTAACCGGGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCAACCAGCACGACCCACCAATCGTAAAGAATgtcac                                                                                 >  2:215935/1‑146 (MQ=14)

                                                                    tGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCg                                                                               >  2:2130966/1‑150 (MQ=255)

                                                                      gTCGCAGGTTCTTATCCTGCTCGACCCTCCAGTTTTTACATCAAACTCAGATGTTAAGCGTGTAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                             <  1:2155168/150‑1 (MQ=17)

                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGGCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                             >  1:479743/1‑150 (MQ=255)

                                                                      gTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCATGACCCACCAATCTTAAAGATTGGCCCCGAg                                                                             >  1:3036375/1‑150 (MQ=255)

                                                                       tCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGt                                                                            >  2:665069/1‑150 (MQ=255)

                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                                                        >  2:2629554/1‑150 (MQ=255)

                                                                           aGGTTCGAATCCTGCACGACCCACCAGTTTTAACAACAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTaaaa                                                                        >  2:2629551/1‑150 (MQ=255)

                                                                             gTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACGCAGATGTTAAGCGGGAAGGATAACGTTGCGACAGCAACGGCCCGAGGGCCGAGCGAAGCCGGGCCTCCGGGACGACCCCCCCATCATAAAGGTTTGCCCCCAGaaaaaaa                                                                      >  2:1047624/1‑149 (MQ=9)

                                                                                 gAATCCTGCACGACCCACCAGTTTTAACATCAACCTCCGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                                  <  1:1766346/150‑1 (MQ=255)

                                                                                   tccgatcACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                <  2:2330895/144‑1 (MQ=255)

                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                <  2:2753481/150‑1 (MQ=255)

                                                                                   aTCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCACCGAGTAAAAATCATTCa                                                                >  1:3201362/1‑150 (MQ=255)

                                                                                        gCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTaa                                                           <  2:3177220/150‑1 (MQ=255)

                                                                                            gACCCACCAGTTTTAACATCGAAGACAGATGTTAAGCGTGTAGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCa                                                                <  2:131579/141‑1 (MQ=14)

                                                                                               ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                                    >  1:1963212/1‑150 (MQ=255)

                                                                                               ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGt                                                    >  1:1720755/1‑150 (MQ=255)

                                                                                               ccaccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAAACGTTCAGGTAACACCCgg                                                    >  2:454288/1‑149 (MQ=255)

                                                                                                caccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCCCCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACACGTa                                                   >  2:2128929/1‑150 (MQ=255)

                                                                                                caccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATAGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTa                                                   >  1:429269/1‑150 (MQ=255)

                                                                                                 accaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTAt                                                  >  1:1359366/1‑150 (MQ=255)

                                                                                                  ccaGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTTTg                                                 <  2:1727006/150‑1 (MQ=255)

                                                                                                   caGTTTTAACATCGAAGACAGATGTTAAGCGTGTTGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCttt                                                                  <  2:1421627/132‑1 (MQ=14)

                                                                                                   caGTTTTAACATCCAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATgg                                                <  2:361202/150‑1 (MQ=255)

                                                                                                     gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGt                                              >  1:2633081/1‑150 (MQ=255)

                                                                                                     gTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCAAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATACTGAACGACCCACCAATCTTAAAGATTGGACCCGAGTAAAAATCTTACAGGTAACAACCGTATCGGt                                              >  1:2632944/1‑150 (MQ=21)

                                                                                                       tttAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCg                                            <  2:195417/150‑1 (MQ=255)

                                                                                                         tAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGtt                                          <  1:1699323/150‑1 (MQ=255)

                                                                                                           ccATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTACAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                                        <  1:897861/149‑1 (MQ=255)

                                                                                                           aCATCACACTACGCTGATAATCGAGCAGGATAACGCTGCGCCAGCACCGGCCGGTCGTGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAg                                        <  2:2341256/150‑1 (MQ=25)

                                                                                                             aTCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCt                                      >  1:1685636/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc                                     >  1:476117/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc                                     >  2:1591004/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc                                     >  1:3113046/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc                                     >  2:3040570/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTc                                     >  2:2319437/1‑150 (MQ=255)

                                                                                                              tCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAACTTAAAGATTGGCCCCGAGTAAAAAACATTCAGGTAACACCCGAATGGGTCGTTaccac                                     >  1:1886103/1‑146 (MQ=25)

                                                                                                               cAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa                                    <  1:375445/150‑1 (MQ=255)

                                                                                                               cAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCa                                    <  2:1777515/150‑1 (MQ=255)

                                                                                                                aaaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTAAAAGATAGGCCACGAGTAAAAATATTTCAGGTAACAAACCTAAGGGACGATAGcaaag                                   >  2:2428547/1‑146 (MQ=18)

                                                                                                                 aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGt                                  <  1:1492155/150‑1 (MQ=255)

                                                                                                                 aaCTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGt                                  <  2:306648/150‑1 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAATATAGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                               >  1:108720/1‑150 (MQ=255)

                                                                                                                    tCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTATCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTgg                               >  1:566011/1‑150 (MQ=255)

                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                             <  1:2084402/150‑1 (MQ=255)

                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                             <  1:2084391/150‑1 (MQ=255)

                                                                                                                      aGATGTTAAGCGTGAAGGATAACGTTGCGACAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTa                             >  2:886872/1‑150 (MQ=255)

                                                                                                                        aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga                           <  1:632160/150‑1 (MQ=255)

                                                                                                                        aTGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTaga                           <  1:144467/150‑1 (MQ=255)

                                                                                                                                cGTGAAGGATAACGTTGCGCCAGCAACGGCCGGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa                   <  2:2497494/150‑1 (MQ=255)

                                                                                                                                cGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa                   <  2:2346098/150‑1 (MQ=255)

                                                                                                                                cGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGa                   <  1:2130966/150‑1 (MQ=255)

                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                 >  1:696676/1‑150 (MQ=255)

                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                 <  1:216088/150‑1 (MQ=255)

                                                                                                                                  tGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACt                 >  1:2406983/1‑150 (MQ=255)

                                                                                                                                   gAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                <  1:2970863/150‑1 (MQ=255)

                                                                                                                                   gAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACtt                <  1:28427/150‑1 (MQ=255)

                                                                                                                                    taGGATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGc                                                                                   >  2:470457/2‑82 (MQ=255)

                                                                                                                                    aaGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACttt               >  1:2923171/1‑150 (MQ=255)

                                                                                                                                       gATAACGTTGCGTCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa            <  2:1534769/150‑1 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTTGAGCAGTTGACTTTTat            >  2:349373/1‑149 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTat            >  1:436641/1‑149 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa            <  1:2162627/150‑1 (MQ=255)

                                                                                                                                       gATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTaa            <  1:434652/150‑1 (MQ=255)

                                                                                                                                        aTAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAt           <  1:284887/150‑1 (MQ=255)

                                                                                                                                            cGTTGCTCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt       <  1:2088236/150‑1 (MQ=255)

                                                                                                                                            cGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAAt       <  2:1000554/150‑1 (MQ=255)

                                                                                                                                                 cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc  >  1:344380/1‑150 (MQ=255)

                                                                                                                                                 cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTc  >  1:1076124/1‑150 (MQ=255)

                                                                                                                                                 cgcCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGAGTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAGTTGGTc  >  1:822754/1‑150 (MQ=255)

                                                                                                                                                   ccAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAAt                                                                                                <  1:2238355/54‑1 (MQ=35)

TCGATCCCGTCATCACCCACCACTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAGTTTTAACATCAAACTCAGATGTTAAGCGTGAAGGATAACGTTGCGCCAGCAACGGCCCGTAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATCTTAAAGATTGGCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTC  >  NC_000913/781122‑781416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 11 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`