Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,286,965 A→T 13.9% intergenic (+439/+101) narI → / ← rttR nitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,286,9650AT13.9% 225.7 / 2.7 104intergenic (+439/+101)narI/rttRnitrate reductase 1, gamma (cytochrome b(NR)) subunit/rtT sRNA, processed from tyrT transcript
Reads supporting (aligned to +/- strand):  ref base A (33/48);  new base T (3/11);  total (42/62)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 104 total aligned reads displayed.

CTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG  >  NC_000913/1286822‑1287111
                                                                                                                                               |                                                                                                                                                  
cTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGACTTCCGGGGCTGTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCg                                                                                                                                              >  2:177661/1‑150 (MQ=255)
cTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGATATTCAGAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTACGCCCTTCGGGTCGGTTCCAGCGGCAACGCGCTCTCGCTGGcgcgcg                                                                                                                                              >  2:177615/1‑150 (MQ=14)
   ccccGCTTGGGCAGAATATTTGATTGGGGATTCGTTTGAGAATTCGGGGTCTTTTGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                           <  2:3070834/150‑1 (MQ=255)
   ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                           <  1:3006293/150‑1 (MQ=255)
   ccccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGc                                                                                                                                           <  1:3006311/150‑1 (MQ=255)
     ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCg                                                                                                                                         >  1:2336854/1‑150 (MQ=255)
     ccGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGACg                                                                                                                                         >  2:1328806/1‑150 (MQ=255)
       gCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                       <  2:2830758/150‑1 (MQ=255)
       gCTTGGGCAGAATATTTGATTGCGGATTAGTTTGAGAATTCCTGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGaa                                                                                                                                       <  2:2830765/150‑1 (MQ=255)
            ggCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTa                                                                                                                                  >  1:2278860/1‑150 (MQ=255)
              cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                <  2:141924/150‑1 (MQ=255)
              cAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGt                                                                                                                                <  1:44376/150‑1 (MQ=255)
               aGAATTTTTGATTGCGGATTCGTTTGAGAATTCCGGTGCTTTTGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTc                                                                                                                               <  1:2958273/150‑1 (MQ=255)
               aGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACACTCGCTGACGCTCGAGCCGAACCGTAGta                                                                                                                               >  1:2769730/1‑149 (MQ=255)
                   tatTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAg                                                                                                                           >  1:1780486/1‑150 (MQ=255)
                     tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGACGAACCTTAGTCGAAGCt                                                                                                                         >  2:159789/1‑150 (MQ=255)
                     tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                         >  2:1809773/1‑150 (MQ=255)
                     tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCt                                                                                                                         >  2:241182/1‑150 (MQ=255)
                     tttGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTCTTCGAAGCt                                                                                                                         >  2:159782/1‑150 (MQ=255)
                     tttGATTGCGGATTCGTTTGAGAATTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCTCTGCGCGCTTCGCCCATCGGGTCGTTGCCTGCCGCAAACCTCTCTCGCTGACGCTCGAGGCGAACCTTAGTCGAAGCt                                                                                                                         >  2:159794/1‑150 (MQ=11)
                       tGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCTCTCACGCAGACGCTCGAGCCCAACCAAAGTCGAAGCAtc                                                                                                                       >  2:2738386/1‑150 (MQ=14)
                          ttGCGGATTCGTTTGAGTATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                    <  1:684027/150‑1 (MQ=255)
                          ttGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATAACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCATTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCa                                                                                                                    <  1:2964839/150‑1 (MQ=255)
                           tGCGGAGTCGTTGGTGTTTTCCGGGTCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCAt                                                                                                                   <  1:2369380/150‑1 (MQ=14)
                            gCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATc                                                                                                                  <  2:3153781/150‑1 (MQ=255)
                                aTTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCACAtacgta                                                                                                              >  2:655386/1‑145 (MQ=255)
                                  tCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGTCCAGCGGCAACCCACACTCGCAGGCCCTCGAGACGAAACTTTGTCGAAGCTTCTCATCCTTccc                                                                                                            >  1:1656445/1‑150 (MQ=11)
                                     tttGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCAACGCGCTCTCGCTGTCGCTCGAGCCGAACCTTGGTCGAACCTTCTCCTCATTCCCCGc                                                                                                         >  2:2569410/1‑150 (MQ=11)
                                       tGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                       >  2:1610184/1‑150 (MQ=255)
                                        gagaATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACCCACACTCGCGGACGCACGAGACCAACCATAGACGAAGCATCACATCCAAcccccccag                                                                                                      >  1:2014602/1‑145 (MQ=14)
                                           aaTTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCATCGCTGCGCCCTACGCCCTTCGCGTCGTTTACTCCGCCAACTCTCTCTCGCTGTCCCACGAGACCAAACATAGTCGAAGCTTCTCATCCTTGCCCGCATGGGc                                                                                                   >  2:1948348/1‑150 (MQ=11)
                                              tcggggtCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCTTCCTTCCCCGCATGGACAGa                                                                                                <  1:3021961/147‑1 (MQ=255)
                                              tcgggggCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGACAGa                                                                                                <  1:3021912/147‑1 (MQ=255)
                                              tCCGGGGCTTTTGCTAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                <  1:2827804/150‑1 (MQ=255)
                                              tCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGa                                                                                                <  1:2040516/150‑1 (MQ=255)
                                                    tcTTTTGTAAGAGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                          <  2:1588721/149‑1 (MQ=255)
                                                    gCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAttt                                                                                          <  2:2307785/150‑1 (MQ=38)
                                                      ttttGTAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                                        <  1:1882140/150‑1 (MQ=25)
                                                      ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACGCACTCAAGCTGACCCACGAGGCCAACCTAAGTCGAAGCATCTCAAACTTCCCCGCCGGGGCCGaagagttta                                                                                        >  1:2327258/1‑143 (MQ=11)
                                                      tttagtaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                                        <  1:28353/144‑1 (MQ=255)
                                                      ttaagtaaGCGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGACGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGa                                                                                        <  1:1882207/144‑1 (MQ=255)
                                                         tGAAAGTGTTGGTGGTGGGTGAAGGATTCCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATTGGCAGAATATTTGATTg                                                                                     <  1:1034056/150‑1 (MQ=255)
                                                         tGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTg                                                                                     <  1:1034073/150‑1 (MQ=39)
                                                                gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTc                                                                              <  2:368850/150‑1 (MQ=37)
                                                                gATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTc                                                                              <  2:368867/150‑1 (MQ=39)
                                                                   ggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCACTCGCTGGCGCGCGAGCCCAACCGTAGTCGAAGCGTCTCATCCGTCCCCGCCGGGGGCGCATATTTGATTGGGGGttgggg                                                                           >  1:1739252/1‑146 (MQ=11)
                                                                    gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                          <  1:1109200/150‑1 (MQ=255)
                                                                    gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                          <  1:1109215/150‑1 (MQ=255)
                                                                    gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCtt                                                                          <  2:2672475/150‑1 (MQ=255)
                                                                      tgtggGGGAAGGATTACTCAGCGCTTCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTga                                                                        <  2:2465896/149‑1 (MQ=255)
                                                                       gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                       <  1:2303820/150‑1 (MQ=255)
                                                                       gcgctctaaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTgag                                                                       <  1:2303829/143‑1 (MQ=255)
                                                                             gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCa                                                                 <  2:3023781/150‑1 (MQ=255)
                                                                                  aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGGTTGGGAGTTCAGGGAc                                                            >  2:2256383/1‑150 (MQ=255)
                                                                                  aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGGTTGAGAGTTCAGGGAc                                                            >  2:3178931/1‑150 (MQ=255)
                                                                                  aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGTGAGTTCAGGGAc                                                            >  2:2256924/1‑150 (MQ=255)
                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCTAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                        <  2:1058274/150‑1 (MQ=255)
                                                                                      cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACtttt                                                        <  2:2309086/150‑1 (MQ=255)
                                                                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCACGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTACCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGaa                                                     >  2:1654697/1‑150 (MQ=255)
                                                                                         aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACTCTCGCTGACGCTCGAGCCGACCCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTGGGCAGGTCAGGGCCTTTTGaa                                                     >  2:1776441/1‑150 (MQ=11)
                                                                                           cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAg                                                   <  1:2580439/150‑1 (MQ=255)
                                                                                                cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtg                                              <  1:316678/150‑1 (MQ=255)
                                                                                                  cgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTAGCTATAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCAGCTTGTGCAGAATATTTGATTGCGGATTCGATTGAGAGTTCAGGGACTTTTGAAAGTGAtggt                                            <  1:2782556/150‑1 (MQ=11)
                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        <  2:725184/150‑1 (MQ=255)
                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        <  2:261203/150‑1 (MQ=255)
                                                                                                      tCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                        <  2:725133/150‑1 (MQ=255)
                                                                                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCTAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCTGAATATTTGATTGCGGATTGGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                       <  2:789674/150‑1 (MQ=14)
                                                                                                       cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                       <  2:789359/150‑1 (MQ=255)
                                                                                                        gCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggg                                      <  1:2853154/150‑1 (MQ=255)
                                                                                                          ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                    <  2:1916115/150‑1 (MQ=255)
                                                                                                          ccTGCGGGCCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTggggg                                    <  2:3037170/150‑1 (MQ=255)
                                                                                                            ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                  <  1:1461318/150‑1 (MQ=255)
                                                                                                             tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                 <  2:1678530/150‑1 (MQ=255)
                                                                                                             tCGGGTCGTTGCCTGCGGCAACGCTCTCTAGCTGCCGCTCGATCCGAAACATAGTCGAAGCATCTCAAACGTCCCCGCATGGGCAGAATAATTGATTGCGGATTAGCTTGGGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                 >  2:698121/1‑150 (MQ=14)
                                                                                                             tCGGGTCGTTGCCTGCGGCAACCCACTCTCGCTGACGCTCGAGCCGAACCATAGTCCAAGCTTCACAACCATACCCCCAAGGGCAGAATATTTGATTTCGGATTCGCTTGGTAGTTAAGGGACTTTTTAAAGTGATTGTGGTGGGGGAAg                                 >  2:1138903/1‑150 (MQ=14)
                                                                                                                ggTCGTTGCCGGCGGCAACGCTCTCACGCTGACCCTCGAGCCGAACCATAGTCGAAGCTTATAATACATACCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGATATTTAAGGGACATTTTAAAGTGATGGTGGTGGGGGAAGGAt                              >  2:1491482/1‑150 (MQ=14)
                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGGCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATGGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                            <  1:409405/150‑1 (MQ=255)
                                                                                                                  tCGTTGCCTGCGGCAACGCTCTCTCGCTGCCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTa                            <  1:409422/150‑1 (MQ=255)
                                                                                                                   cGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAc                           <  1:1488020/150‑1 (MQ=255)
                                                                                                                   cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTCGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAc                           <  1:3098842/150‑1 (MQ=255)
                                                                                                                   cGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAc                           <  1:1488018/150‑1 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTATCATCCATCCCCCCCTGGGCAGAATATTTTATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGTAGGATTaaaa                         >  1:1147073/1‑147 (MQ=14)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCGTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCGTGTGAGTTCAGGGACTTTTGGAAGTGATGGTGGTGGGGGAAGGATTACTc                         >  1:184844/1‑150 (MQ=255)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCTGACGCACGAGACGAACCATACTCGAAGCATCACATCCATCCCCGCAAGGGCAGCATATTTGATTTCGGATTCGATTGTGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTaata                         >  1:2575796/1‑147 (MQ=14)
                                                                                                                     ttGCCTGCGGCAACGCTCTCTCGCGGACGCTCGAGCCGAACCGTAGTCGAAGCGTCTCATCCGTCCCCGCAGGGGCCGAATATTTGATTGCGGATTCGCGTGAGAGTTCAGGGACATTTGAAAGTGATGGGGGTGGGGGCAGGATTACTc                         >  1:529451/1‑150 (MQ=17)
                                                                                                                     ttGCCTGCGGCAACGCTCTCACGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCATCCCCCCATGGGCAGAATATTTGATTTCGGATTAGCTTGAGAGTTCAGGGACTTTTGAAATTGATGGTGGTGGGGGAAGGATTACta                         >  1:2575916/1‑149 (MQ=17)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                      >  2:2728286/1‑150 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                      <  2:1679396/150‑1 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAgc                      <  2:3196683/150‑1 (MQ=255)
                                                                                                                        ccTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGGGATGGTGGTGGGGGAAGGATTACTCAgc                      >  2:2346016/1‑150 (MQ=255)
                                                                                                                               cAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAGTCCTCAGCGCTgcgc               >  2:1714479/1‑150 (MQ=255)
                                                                                                                                 aCGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTgcgcgc             <  1:144478/150‑1 (MQ=255)
                                                                                                                                  cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt            >  1:3022397/1‑150 (MQ=255)
                                                                                                                                  cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt            >  1:3022410/1‑150 (MQ=255)
                                                                                                                                  cGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGAGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCt            >  1:2237474/1‑150 (MQ=255)
                                                                                                                                      ctctcGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGc        <  1:2665317/150‑1 (MQ=255)
                                                                                                                                       tctcGCTGTCGCTCGAGCCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGcc       <  1:3172928/150‑1 (MQ=255)
                                                                                                                                           gCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTc   <  1:1610184/150‑1 (MQ=255)
                                                                                                                                            cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg  <  2:715163/150‑1 (MQ=255)
                                                                                                                                            cTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCg  <  2:2868989/150‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                  
CTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTTGAGAATTCCGGGGCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGACGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCG  >  NC_000913/1286822‑1287111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: