Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 9.2% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 9.2% 344.3 / 22.1 136noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (56/64);  new base A (6/6);  total (62/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.28e-01

Only 100 of 136 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287001‑1287297
                                                                                                                                                    |                                                                                                                                                    
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                     <  2:151196/150‑1 (MQ=255)
ttCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                     <  2:151342/150‑1 (MQ=255)
gtCCCCGCCTGGGCAGCATATTTGATTGCGGAGTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                     <  1:2738386/149‑1 (MQ=255)
 tCCCCGCATGGGCAGAATATGTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTGTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCATCTCCCGTCGTGTAGATTCCTCAGCCAACGCTCTCTCGCGTGCGCACGAg                                                                                                                                                    >  2:2390315/1‑150 (MQ=14)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCACGAGTCg                                                                                                                                                 >  1:3219054/1‑150 (MQ=255)
      gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                               >  1:3139441/1‑150 (MQ=255)
      gCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGaa                                                                                                                                               >  1:2125729/1‑150 (MQ=255)
          gggCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTGCCTGAGGCAACGGTCTCTCGCTTTCGCTCGAGTCGAACCtt                                                                                                                                           >  2:575901/1‑150 (MQ=14)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCACACACGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                         >  1:2751969/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                        <  1:1491482/150‑1 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCAAGCTGGCGCTCGAGTAGAACCTTGGta                                                                                                                                       >  1:2155499/1‑149 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                       >  2:2695814/1‑150 (MQ=255)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCACTCAAGCTGGCGCTCGAGTCGAACCGTGGTc                                                                                                                                       >  1:1281334/1‑150 (MQ=14)
               gAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGAACCGGGGTCg                                                                                                                                      >  1:584074/1‑150 (MQ=255)
                    tttGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGca                                                                                                                                 >  2:2852237/1‑149 (MQ=255)
                     ttGATTTCGGATTCGCTTGAGATTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                                <  1:655386/150‑1 (MQ=255)
                          tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCAGGGGCACCGCACTCAAGCTGGCGCGCGAGTCGGACCCTGGGCGAAACttataaa                                                                                                                           >  1:2993730/1‑145 (MQ=14)
                          tGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTAATCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCCCTGGCGCTCGAGTCGAACCTTGGGCGAAGCTTCTCaa                                                                                                                           >  2:2860454/1‑149 (MQ=255)
                            cGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCGCACTCGCAGGCGCACGAGTCGAACCGAGGTCCAAGCTTCAAATcc                                                                                                                         >  1:2970066/1‑150 (MQ=14)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                  <  2:1155219/150‑1 (MQ=255)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCg                                                                                                                  <  2:2806851/150‑1 (MQ=255)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCCGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAATCTTCTAAACCTTCCCCg                                                                                                                  >  2:1758988/1‑150 (MQ=14)
                                   gCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCTCTCGCTGGCGCGCGAGTCGAACCTTGGTCGAAGCTTATCATACATCCCCg                                                                                                                  >  2:382908/1‑150 (MQ=14)
                                      tGAGAGTTCCGGGCCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGATCCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                               <  1:2131496/150‑1 (MQ=255)
                                      tGAGAGTTCAGGGCCTTTTGTATGTGCTGGTGGTGGTGGATGTATTACGCCTCGCCGCGCGATTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                               <  1:313983/150‑1 (MQ=14)
                                          agTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGGTCGTTGCCTGGGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                           <  2:2237474/150‑1 (MQ=255)
                                          agTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                           <  2:3022397/150‑1 (MQ=255)
                                          agTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGc                                                                                                           <  2:3022410/150‑1 (MQ=255)
                                               aGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCAGCGGCAACGCACACTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCATATCAACCTTCCCCGCAGGGGCCGaaa                                                                                                      >  1:2720310/1‑149 (MQ=14)
                                                  gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAAAGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGCATCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                   >  2:1349606/1‑150 (MQ=255)
                                                  gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTACGGGTCGTTGCCTGCGGCAACCCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATAtt                                                                                                   >  2:2078983/1‑150 (MQ=255)
                                                  gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCACTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTCGAAGAATCTCATCCTTCCCCGCATGTGCAGAATAtt                                                                                                   >  2:2698749/1‑150 (MQ=11)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCTCCCTTCGGTTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                >  2:1876620/1‑150 (MQ=255)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                >  2:2228321/1‑150 (MQ=38)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACACAGCGCTGCGCGCATCGCCCTTCGGGTAGTTGCCTGACGCAACGCTCTCTCGCTGGCGCTCGAGTCTAACCTTGGTCGAAGCTTATAATCCTTCACCGCATTGGCACAAAATTTaa                                                                                                >  2:1874978/1‑150 (MQ=11)
                                                      tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCACGCTGGCCCGCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCCCAGGGGCAGAAAATTTAAt                                                                                               >  2:2296083/1‑150 (MQ=14)
                                                       ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACATGGTCGAAGCATCTCAACCATCCCCGCAAGGGCAGAATaattaatt                                                                                              >  2:159142/1‑150 (MQ=14)
                                                                 tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                                     >  2:2973331/1‑101 (MQ=32)
                                                                 tggtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGa                                                                                                                                     <  1:2973304/101‑1 (MQ=32)
                                                                         ggggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCACAATATTTAATTTCGGAAGCGTTgggccgg                                                                            >  2:1023101/1‑146 (MQ=14)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                        <  1:177661/150‑1 (MQ=255)
                                                                             aaGGACTACTCAGCGCCGCGCGCTACGCCCTTCGGGTCGTTGCCTGCGTCAACGCTCTCTCGCTTGCGATCGTGGCGAAGCTTTGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAg                                                                        <  1:177615/150‑1 (MQ=11)
                                                                                 aTTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCCGAATCATTTATTGCGGATTCGGTTGGGAGGTCAGGGAc                                                                    >  2:2256408/1‑150 (MQ=11)
                                                                                    aCTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACttt                                                                 <  1:682311/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                <  2:2014602/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                <  1:241182/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                <  1:159794/150‑1 (MQ=255)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                <  1:159782/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                               >  2:2057932/1‑150 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCGGCGGCAACGCTCTCGCGCGGGCGCGCGGGTCGCACCGTGGGCGGAGCGTATCAACCGTCCCCCCCGGGGCAGCGTATTTAATTTCGGGTTGGGTGGGGAGGTCAGGGGCtggtgtg                                                             >  2:1382992/1‑144 (MQ=2)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTAATTGCGGATTCGTTTGGAAGTTCAGGGACTTTTGaa                                                             >  2:2433191/1‑150 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCgg                                                                                          <  1:101322/121‑1 (MQ=38)
                                                                                           gcTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCGCACTCCCAGGCGCACGAGTCGAACCATGGTCGAAGCTTCTACACCTTCCCCCCCGGGGCAGAAAATTTAATTTCGGCTTCGGTGGGGAGGTCCGGGACGGTTGAAAGt                                                          >  2:831569/1‑150 (MQ=9)
                                                                                            cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                        >  2:2301424/1‑71 (MQ=34)
                                                                                            cTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGt                                                                                                                                        <  1:2301424/71‑1 (MQ=34)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTg                                                                                             <  1:2102015/113‑1 (MQ=39)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                        <  1:2228321/150‑1 (MQ=255)
                                                                                                 cgcTTCGCCCTTCGGGTCGTGTGCGGCGGCCACCCGCTCTAGCTGGGGCACAAGTCGCAACTGGGTCGAAGCGTATCAAACATACCCCCCAGGGCCGTAAAATTTATTGCGGAGTGGGTGGGGAGTTGAGGGGCGTTTGTAAGTggtggt                                                    >  2:1380882/1‑150 (MQ=2)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTaa                                                                                                >  2:2962781/1‑103 (MQ=14)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtgg                                               <  1:324357/150‑1 (MQ=255)
                                                                                                         ccTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGGATGGGCCGAAGATTTAATTTCGGATACGTTGTTCCGTTCAGGGACTTTTGCAAGTGGTGGTGGTggggg                                            >  2:1819993/1‑150 (MQ=14)
                                                                                                          cTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGa                                           <  1:2433191/150‑1 (MQ=255)
                                                                                                           ttCGGGTGGGTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                          <  1:3000513/150‑1 (MQ=255)
                                                                                                           ttCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGaa                                          >  2:1811117/1‑150 (MQ=255)
                                                                                                                gTCGTTGCCAGCGGCAACGCTCTCGCGCTGGCGCTCCAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATATAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGAtt                                     <  1:1380882/150‑1 (MQ=255)
                                                                                                                 tCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTc                                    <  1:1027295/150‑1 (MQ=255)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGa                                  <  2:1656445/150‑1 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCAGGGGCAGAATATTTAATTGCGGATTCGTGGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGGGGGGGCAGGATTCgcc                                 >  2:2491379/1‑148 (MQ=17)
                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTAGAAGCTTCTCATGCTTCCCCGCATGGGCAGAATATTTAATTGCGTATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGTATTCGAAcc                               <  1:698121/150‑1 (MQ=17)
                                                                                                                      gCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGCCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAcc                               <  1:1138903/150‑1 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAATATTGAATTGCGGATGCGTTGGGACGTTCAGGGACTTTTGAAAGTGGTGGTGGTGGGGGAAGGATTCGAACCt                              >  2:2886353/1‑150 (MQ=17)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                               <  1:790430/101‑1 (MQ=38)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                               >  2:790430/1‑101 (MQ=38)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                               <  1:871023/101‑1 (MQ=38)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                               <  1:376936/101‑1 (MQ=38)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                               <  1:3236448/101‑1 (MQ=38)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCAACCATACCCCCAAGGGCAGAAAATTTAATTGCGGGTTCGTTGGGGAGTTCAGGGACTTTTTAAATTGATGGTGGTGGGGGGAGGATTGGAAccat                             >  1:2641192/1‑148 (MQ=14)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTg                                                                                                                                          <  1:2471032/40‑1 (MQ=40)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCAGAAAATTGAATATCTCAGACTGTGTGAAGGACAGGTACTTTTGAATGTGATGGTGGTGGGGGAAGGATTAGCACCTTc                            >  2:529019/1‑150 (MQ=14)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                      <  1:1833237/121‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                         <  2:1156134/150‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCGAGGCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGaa                         <  1:1328806/150‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                      <  1:1833218/121‑1 (MQ=255)
                                                                                                                            ggCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCTTGGGCAGAATATTTGATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                      >  2:1833218/1‑121 (MQ=255)
                                                                                                                               aaCGCTCTCTCGCTGCCGCACGAGCCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGTGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCATGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTc                      <  2:2327258/150‑1 (MQ=17)
                                                                                                                                        tcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTAAAAACCAAACCCCCAAGGGCAGAAAAATTAAATGCGGATTAGTTTGGAAGTTCATGGACTTTTGAAAGTTATGTTGGTGTGGGAATGATTCGAAACATCGAAGTCGATGACGGc             >  1:2455880/1‑150 (MQ=16)
                                                                                                                                         cggtGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCACCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa            <  1:149663/147‑1 (MQ=255)
                                                                                                                                         cGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCa            <  1:149623/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa          <  1:1382992/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa          <  1:1382889/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  2:2447353/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  1:159142/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  1:1811117/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  1:2078983/150‑1 (MQ=255)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa    <  1:1200045/150‑1 (MQ=255)
                                                                                                                                                  tCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCCCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAg   >  1:824802/1‑150 (MQ=255)
                                                                                                                                                  tCGAGTCGAACCTTGGTCGAAGCTTCTCAACCAACCCCGCAAGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGGGGGGGGGGAAGGCTTCGAACCAACGACGTCGATGACCGCAGATTTACAg   >  1:3031433/1‑150 (MQ=18)
                                                                                                                                                   cGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt  <  1:3178931/150‑1 (MQ=255)
                                                                                                                                                   cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt  <  1:2256408/150‑1 (MQ=255)
                                                                                                                                                   cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt  <  1:2256383/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGT  >  NC_000913/1287001‑1287297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: