Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,234 C→A 17.0% intergenic (‑30/+278) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2340CA17.0% 352.5 / 11.1 159intergenic (‑30/+278)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (52/67);  new base A (24/10);  total (82/77)
Fisher's exact test for biased strand distribution p-value = 6.57e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 161 total aligned reads displayed.

GGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177088‑2177383
                                                                                                                                                  |                                                                                                                                                     
ggCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaa                                                                                                                                                    <  2:1126402/150‑1 (MQ=255)
 gCAGCGGTTTCTCCCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                   <  2:2144790/150‑1 (MQ=255)
 gCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCaaaa                                                                                                                                                   <  2:858497/150‑1 (MQ=255)
     cGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATATTCATATCCTGTCGTTTGTTTTCGATTTCAAAAtata                                                                                                                                               >  2:1595111/1‑150 (MQ=255)
      ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATaa                                                                                                                                              <  2:2868947/150‑1 (MQ=255)
      ggTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCAATTTCAAAATATaa                                                                                                                                              <  2:1200196/150‑1 (MQ=255)
          tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          >  1:2982164/1‑150 (MQ=255)
          tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                          >  1:2982163/1‑150 (MQ=255)
             accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                       >  2:2087293/1‑150 (MQ=255)
              ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAatta                                                                                                                                      >  1:1737590/1‑150 (MQ=255)
              ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAaata                                                                                                                                      >  1:1104105/1‑150 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTGAAAATATAATTAAattat                                                                                                                                     >  1:333505/1‑150 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     >  1:333496/1‑150 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                     >  2:1754342/1‑150 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAaatat                                                                                                                                     >  1:2281307/1‑150 (MQ=255)
               caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAattat                                                                                                                                     >  1:3206351/1‑150 (MQ=255)
                accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    >  2:230348/1‑150 (MQ=255)
                accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    >  2:215584/1‑150 (MQ=255)
                accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    >  1:489751/1‑150 (MQ=255)
                accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAattatt                                                                                                                                    >  1:489745/1‑150 (MQ=255)
                accacTTGCATCGTTTCGAGATCGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                    <  1:1314112/150‑1 (MQ=255)
                 ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                   >  2:817899/1‑150 (MQ=255)
                    cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                <  2:2296451/150‑1 (MQ=255)
                       gCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGttttt                                                                                                                             <  2:294502/150‑1 (MQ=255)
                        cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  1:255671/150‑1 (MQ=255)
                        cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                            <  1:986380/150‑1 (MQ=255)
                         aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                           <  1:633467/150‑1 (MQ=255)
                         aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                           <  1:205171/150‑1 (MQ=255)
                         aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                           <  2:2438670/150‑1 (MQ=255)
                         aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTaa                                                                                                                           >  2:584506/1‑150 (MQ=255)
                             tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  1:1930760/150‑1 (MQ=255)
                             tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                       <  1:1424090/150‑1 (MQ=255)
                                cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCga                                                                                                                    >  1:1568355/1‑150 (MQ=255)
                                         gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                           <  2:1409266/150‑1 (MQ=255)
                                              ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATTAAAATATTTTTTTTTTAATATCGAGATAACGCTAACaa                                                                                                      >  2:2529401/1‑150 (MQ=255)
                                              ttGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaa                                                                                                      <  2:605537/150‑1 (MQ=255)
                                               tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaa                                                                                                     >  2:2328061/1‑150 (MQ=255)
                                                 aaagCCGCAACCGCATAACCGCCGCGCAGTGCGAAGAACACCATCTGCATTCACGATACCACCAACAATTTCATATCCTCTCGATAGATTTCGAATTCAAAATATAATCAAATTATTTCTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                   <  2:1668197/147‑1 (MQ=255)
                                                   tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaaag                                                                                                 >  1:1044046/1‑148 (MQ=255)
                                                     ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTGCGGGTTCAAAATATAATGAAAGAATTTTTTTTTAAATATCGCGATAACCAGCACCAAAAcccc                                                                                               >  1:650409/1‑147 (MQ=255)
                                                      cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACa                                                                                              >  1:1356362/1‑150 (MQ=255)
                                                         aaCCACATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAAATAAATTATATGTTTTTAAATATAGAGATAAAGATAACAAAAAAGACCata                                                                                           >  1:780013/1‑150 (MQ=255)
                                                          aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTTAATTATTTGTTTTTATATATAGAGATAAAGATCACAAAAACCACAatat                                                                                          >  1:2344083/1‑150 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGAATTAAAAATAAAATGAAAATATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAAAATGaaa                                                                                      >  2:3134305/1‑150 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTaaa                                                                                      >  2:431199/1‑150 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAAAATGaaa                                                                                      >  1:1708198/1‑150 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAAAGTAATTATTTGTTTTTAAATATCGAGATAAAGATAAAAAAAAAGACAAAATTaaa                                                                                      >  1:1896407/1‑150 (MQ=255)
                                                              cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTATTTGTTTTTAAAAATCGAGATAAAGATAACAAAAAAGACAAAATTaaa                                                                                      >  1:1503236/1‑150 (MQ=255)
                                                                  aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                  >  2:3166962/1‑150 (MQ=255)
                                                                   ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAATAAAGTAAATATTTTTTTTTTAATATAGAGATAAAGAAAAAAAAAAAAACAAAATGAAAaatat                                                                                 >  1:1167895/1‑150 (MQ=255)
                                                                     gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                               <  2:904029/150‑1 (MQ=255)
                                                                     gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTGAAAATATAAATTAAATATTTTTTTTTTAAAATGGAGCTAAAGAACGAAAAAAACACAAAATTTAAaatattt                                                                               >  1:1428292/1‑149 (MQ=255)
                                                                       cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                             <  2:351064/150‑1 (MQ=255)
                                                                       cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATTAAAATTATTCgg                                                                             >  2:353390/1‑149 (MQ=255)
                                                                           cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTTAAAATAGAGATAAAGATCAAAAAAAAGACAATATTAAAATTATTAgaggag                                                                         >  1:456635/1‑150 (MQ=255)
                                                                              tgCGTTGTTCAGCATCTGCTTTGTCGATACCCCGAACATTTTCATCTCCTGTGTTTTTTTTTCTATTACAAAATAAAATTAATTTTTTTTTTTTTAATTATCTAGATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGa                                                                      <  1:1920301/150‑1 (MQ=255)
                                                                                cttttcccAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  2:3051828/143‑1 (MQ=255)
                                                                                cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                    <  2:1036794/150‑1 (MQ=255)
                                                                                 gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAAAATTAAAATTAATGGAGGGGTGAAAg                                                                   >  1:778464/1‑150 (MQ=255)
                                                                                 gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGATATAAACATCACAAAAAAGAAAATATTAAAATAATAAGATGAGTTAAAg                                                                   >  1:1804821/1‑150 (MQ=255)
                                                                                   tgttCAGCATCTGCTTTTTCGATACCACTTACATTTTCATATCCTTTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                 <  2:2595131/150‑1 (MQ=255)
                                                                                   tgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                 <  2:515863/150‑1 (MQ=255)
                                                                                    gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                <  2:1447668/150‑1 (MQ=255)
                                                                                     ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAAACATCACAAAAAAGACAAAATGAAAATTATTAGAGGGGTGTAATGCaa                                                               >  2:684122/1‑150 (MQ=255)
                                                                                     ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAAAACAAAAAAGACAATATTAAAATTATTATAGGAGTGAAAGGcca                                                               >  2:2807672/1‑148 (MQ=255)
                                                                                         gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGTATTAAAAATATAAATTAAAAATTTTTTTTTATATATAAATATAAACATAAAAAAAAAACAAATAATAAAATTATTAGAAGATTGGAATGGaaaaaa                                                           >  1:2619285/1‑150 (MQ=255)
                                                                                         gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAAAAAAGAGATAAAGCAAAAAAAAAAAAAAAAATTAAAATTATTAGAGGGGGGTAAGGCaaaaaa                                                           >  1:1676585/1‑150 (MQ=255)
                                                                                         gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAAATAAATAAAATTTTTTTTTTTTAATAACAATATAAAGATAACAAAAAACAAAAAATTAAAAATATTATAAGAGTGGAATGCaaaaaa                                                           >  1:2619183/1‑150 (MQ=255)
                                                                                          cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                          <  2:2668505/150‑1 (MQ=255)
                                                                                           aTCGGCTTTGTCGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                         <  2:2288245/150‑1 (MQ=255)
                                                                                            tCTGCTTTGTCTATACCACGTACTTTTTCATATCATTTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                        <  2:1836536/150‑1 (MQ=255)
                                                                                             cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAAATAAAATATTTTTTTTTTAATATAAAGATAAAGATAAAAAAAAAGACAAAATTAAAAATATTAGAGGGGTGAAAGGCaaaaaaaaga                                                       >  1:2439275/1‑147 (MQ=255)
                                                                                               gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTAGAGGTGTGTAAGGCAAAAAAACGGcc                                                     >  2:3046331/1‑150 (MQ=255)
                                                                                                   tGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAAAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                 <  2:1369806/150‑1 (MQ=255)
                                                                                                   tGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                 <  2:1543387/150‑1 (MQ=255)
                                                                                                     tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                               <  2:816901/150‑1 (MQ=255)
                                                                                                       taTACCACTTAATTTTTAATTTCTTTTTTTTTTTTTTCTATTTAAAAATATATTTAATTTATTTTTTTTTAATTTTCTATATAACTAAAAAAAAAACGAAAATATTAAATTTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATa                                             <  1:2219702/149‑1 (MQ=255)
                                                                                                             aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       >  2:488396/1‑150 (MQ=255)
                                                                                                             aCGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                       >  2:2831113/1‑150 (MQ=255)
                                                                                                                tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGAGAGGGAAGCCg                                    >  2:1954964/1‑150 (MQ=255)
                                                                                                                       taatatCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                             <  1:2397653/148‑1 (MQ=255)
                                                                                                                          tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGCGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                          >  2:1343971/1‑150 (MQ=255)
                                                                                                                          tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                          >  2:1344028/1‑150 (MQ=255)
                                                                                                                             ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                       <  2:89627/150‑1 (MQ=255)
                                                                                                                               tGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                     >  2:3037123/1‑150 (MQ=255)
                                                                                                                                  cGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                  <  2:2560593/150‑1 (MQ=255)
                                                                                                                                   gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                 >  1:2987692/1‑150 (MQ=255)
                                                                                                                                       gtttTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTAGTTAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:1104105/150‑1 (MQ=255)
                                                                                                                                       gtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  1:215584/150‑1 (MQ=255)
                                                                                                                                       gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt             <  2:1896407/150‑1 (MQ=255)
                                                                                                                                        ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg            >  1:1708010/1‑150 (MQ=255)
                                                                                                                                         tttCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc           <  1:612094/150‑1 (MQ=255)
                                                                                                                                          ttagATTTCAAAATATAATGACATTATTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGTGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  1:1875513/147‑1 (MQ=255)
                                                                                                                                          ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc          <  1:174211/150‑1 (MQ=255)
                                                                                                                                           tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  1:2430774/150‑1 (MQ=255)
                                                                                                                                           tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg         <  2:268969/150‑1 (MQ=255)
                                                                                                                                            cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg        <  1:3166962/150‑1 (MQ=255)
                                                                                                                                             gATTTCAAAATATAATGAAACTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGACAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTGTTTGTGCCGGa       >  1:858267/1‑150 (MQ=255)
                                                                                                                                              aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt      >  2:1630700/1‑150 (MQ=255)
                                                                                                                                                  cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg  >  2:1903840/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
GGCAGCGGTTTCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGG  >  NC_000913/2177088‑2177383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: