Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,245 G→T 14.7% intergenic (‑41/+267) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2450GT14.7% 382.8 / 13.9 174intergenic (‑41/+267)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (70/71);  new base T (22/8);  total (92/82)
Fisher's exact test for biased strand distribution p-value = 2.56e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 176 total aligned reads displayed.

TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NC_000913/2177098‑2177393
                                                                                                                                                   |                                                                                                                                                    
tCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaa                                                                                                                                                    >  1:2982164/1‑150 (MQ=255)
 cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                   <  1:2149017/150‑1 (MQ=255)
 cTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGaaa                                                                                                                                                   <  1:1665525/150‑1 (MQ=255)
   accaccACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAatt                                                                                                                                                 >  2:2087293/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAatta                                                                                                                                                >  1:1737590/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATaattaaatta                                                                                                                                                >  1:821848/1‑150 (MQ=255)
    ccaccaCTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAATTAAaata                                                                                                                                                >  1:1104105/1‑150 (MQ=255)
     caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                               >  1:333496/1‑150 (MQ=255)
     caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattat                                                                                                                                               >  2:1754342/1‑150 (MQ=255)
     caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAATGAAaatat                                                                                                                                               >  1:2281307/1‑150 (MQ=255)
     caccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTACAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAattat                                                                                                                                               >  1:3206351/1‑150 (MQ=255)
      accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                              >  2:230348/1‑150 (MQ=255)
      accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                              >  2:215584/1‑150 (MQ=255)
      accacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                              >  1:489751/1‑150 (MQ=255)
      accacTTGCATCGTTTCGAGATCGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAattatt                                                                                                                                              <  1:1314112/150‑1 (MQ=255)
       ccacTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTAttt                                                                                                                                             >  2:817899/1‑150 (MQ=255)
         acTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAAATAAAATAttttt                                                                                                                                           >  1:2592940/1‑148 (MQ=255)
          cTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGtt                                                                                                                                          <  2:2296451/150‑1 (MQ=255)
              cATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTa                                                                                                                                      <  1:986380/150‑1 (MQ=255)
               aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTaa                                                                                                                                     <  2:2438852/150‑1 (MQ=255)
               aTCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAATTATTTTTTTTTaa                                                                                                                                     >  2:584506/1‑150 (MQ=255)
                   tttCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAatat                                                                                                                                 <  1:1424090/150‑1 (MQ=255)
                      cGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCga                                                                                                                              >  1:1568355/1‑150 (MQ=255)
                             gtgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACg                                                                                                                       >  2:504632/1‑150 (MQ=255)
                              tgAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATAAAATGAAAATATTTTTTTTTAAATATCGAGATAAAGa                                                                                                                      >  2:143303/1‑150 (MQ=255)
                               gAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGAt                                                                                                                     <  1:391189/150‑1 (MQ=255)
                                     tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaa                                                                                                               >  2:2328061/1‑150 (MQ=255)
                                       aaagCCGCAACCGCATAACCGCCGCGCAGTGCGAAGAACACCATCTGCATTCACGATACCACCAACAATTTCATATCCTCTCGATAGATTTCGAATTCAAAATATAATCAAATTATTTCTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                             <  2:1668197/147‑1 (MQ=255)
                                         tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaaag                                                                                                           >  1:1044046/1‑148 (MQ=255)
                                           ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTGCGGGTTCAAAATATAATGAAAGAATTTTTTTTTAAATATCGCGATAACCAGCACCAAAAcccc                                                                                                         >  1:650409/1‑147 (MQ=255)
                                            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                        >  2:522759/1‑150 (MQ=255)
                                            cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAAAGACa                                                                                                        >  1:1356362/1‑150 (MQ=255)
                                               aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAAAGAAATTATTTTTTTTTTAATATAGTGATAAAGATCACAAAAAACACAata                                                                                                     >  1:89627/1‑150 (MQ=255)
                                                aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTTAATTATTTGTTTTTATATATAGAGATAAAGATCACAAAAACCACAatat                                                                                                    >  1:2344083/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGACATAACGATCACAAAAAAGACAATATGaaa                                                                                                >  2:3134612/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTaaa                                                                                                >  2:431199/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATTTAATTATTTGTTTTTAAATATAGAGATAAAGATCACCAAAACGACAATATTaaa                                                                                                >  1:486498/1‑150 (MQ=255)
                                                    cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTAAAAATATAAAGTAATTATTTGTTTTTAAATATCGAGATAAAGATAAAAAAAAAGACAAAATTaaa                                                                                                >  1:1896407/1‑150 (MQ=255)
                                                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                           <  2:325215/150‑1 (MQ=255)
                                                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                           <  2:1088746/150‑1 (MQ=255)
                                                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                           <  2:610783/150‑1 (MQ=255)
                                                         ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAATAAAGTAAATATTTTTTTTTTAATATAGAGATAAAGAAAAAAAAAAAAACAAAATGAAAaatat                                                                                           >  1:1167895/1‑150 (MQ=255)
                                                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                         <  2:904029/150‑1 (MQ=255)
                                                           gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGTTTTCAAAATATAAATAAAATATTTTTTTTTTAATATAGTGATAACGATAACAAAAACGACACAATTAAAattttta                                                                                         >  1:3037694/1‑149 (MQ=255)
                                                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                       <  2:351064/150‑1 (MQ=255)
                                                             cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATTAAAATTATTCgg                                                                                       >  2:353390/1‑149 (MQ=255)
                                                                 cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATTGAATTATTTTTTTTTTAATATAGTGAGAAAGATGACAAAAAAAACAAAAATAAAATTATTAgaggtg                                                                                   >  1:1500129/1‑148 (MQ=255)
                                                                  tgtgCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAAAGAAATTAATTGTTTTTAAAAATCGAGATAACCAACACAAAAACCCCAATAAGAAAAATATACcaggaga                                                                                  >  2:2645647/1‑149 (MQ=255)
                                                                    tgCGTTGTTCAGCATCTGCTTTGTCGATACCCCGAACATTTTCATCTCCTGTGTTTTTTTTTCTATTACAAAATAAAATTAATTTTTTTTTTTTTAATTATCTAGATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGa                                                                                <  1:1920301/150‑1 (MQ=255)
                                                                      cttttcccAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                              <  2:3051828/143‑1 (MQ=255)
                                                                      cGTTGTACCGCACCTGCTTTGACGATACCCCGTACATTTTCATATCCTGTCTTTTGTTTTCGATTAAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                              <  2:3051811/150‑1 (MQ=255)
                                                                       gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAAAATTAAAATTAATGGAGGGGTGAAAg                                                                             >  1:778464/1‑150 (MQ=255)
                                                                         tgttCAGCATCTGCTTTTTCGATACCACTTACATTTTCATATCCTTTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                           <  2:2595131/150‑1 (MQ=255)
                                                                          gttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCa                                                                          <  2:1447668/150‑1 (MQ=255)
                                                                           ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAAAACAAAAAAGACAATATTAAAATTATTATAGGAGTGAAAGGcca                                                                         >  2:2807672/1‑148 (MQ=255)
                                                                            tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAAAATCGAGATAACGATCAAAAAAAAGACAATATGAAAATTATTAGAGGCGTGGAAGGCaaa                                                                        >  2:2826417/1‑150 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGTATTAAAAATATAAATTAAAAATTTTTTTTTATATATAAATATAAACATAAAAAAAAAACAAATAATAAAATTATTAGAAGATTGGAATGGaaaaaa                                                                     >  1:2619285/1‑150 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAAAATTAAAATATATTATTTTTAATATATATATAAACAAAAAAAAAAAGACAAAAATATAAATATTAGAGGGGTGTAAAGCagaaaa                                                                     >  1:2486379/1‑150 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAAAAAAGAGATAAAGCAAAAAAAAAAAAAAAAATTAAAATTATTAGAGGGGGGTAAGGCaaaaaa                                                                     >  1:1676585/1‑150 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAAATAAAATATTTTTTTTTTAATAAAGAGATAACAATAACAAAAAACAAAAAAATAAAATTATAAGATGAGTGTAATGCcaaaaa                                                                     >  1:1374782/1‑150 (MQ=255)
                                                                               gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTTAAAATATAGATAAACATAAAAAAAAAAAAAAAAATAAAAAAAATAGAGGGGGGTAAGGCaaaaaa                                                                     >  1:17256/1‑150 (MQ=255)
                                                                                cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAACGCaaaaaaa                                                                    >  2:2936595/1‑150 (MQ=255)
                                                                                 aTCGGCTTTGTCGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                   <  2:2288245/150‑1 (MQ=255)
                                                                                         tGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAAAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                           <  2:1369806/150‑1 (MQ=255)
                                                                                         tGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                           <  2:1543387/150‑1 (MQ=255)
                                                                                             taTACCACTTAATTTTTAATTTCTTTTTTTTTTTTTTCTATTTAAAAATATATTTAATTTATTTTTTTTTAATTTTCTATATAACTAAAAAAAAAACGAAAATATTAAATTTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATa                                                       <  1:2219702/149‑1 (MQ=255)
                                                                                                      tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGAGAGGGAAGCCg                                              >  2:1954964/1‑150 (MQ=255)
                                                                                                        aaTTTTCTTATCCTGTCTTTTTTTTTCTATTACAAAATAAAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                            <  1:2045937/149‑1 (MQ=255)
                                                                                                        aaTTTACATATCCTTTCTTTTTTTTTCTATAAAAAAATATAATAAAATTATTTTTTTAAAAATATCCACAAAACGAACACAAAAACGACAATATTAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                            <  1:1765132/149‑1 (MQ=255)
                                                                                                             tcttttCCTGTCTTTTGTTTTCGATTAAAAATTATAATTAATTTATTTGTTTTTAAATTTCTATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                       <  1:2397725/145‑1 (MQ=255)
                                                                                                                tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                    >  2:1344028/1‑150 (MQ=255)
                                                                                                                   ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                 <  2:89627/150‑1 (MQ=255)
                                                                                                                     tGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                               >  2:3037123/1‑150 (MQ=255)
                                                                                                                         gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                           >  1:2987642/1‑150 (MQ=255)
                                                                                                                           ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                         >  2:2556519/1‑150 (MQ=255)
                                                                                                                             gtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                       <  1:215584/150‑1 (MQ=255)
                                                                                                                             gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                       <  2:1896407/150‑1 (MQ=255)
                                                                                                                              ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                      >  1:1708010/1‑150 (MQ=255)
                                                                                                                               tttCGTTTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                     <  1:612346/150‑1 (MQ=255)
                                                                                                                               tttCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                     <  1:612094/150‑1 (MQ=255)
                                                                                                                                ttagATTTCAAAATATAATGACATTATTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGTGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                    <  1:1875513/147‑1 (MQ=255)
                                                                                                                                 tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                   <  2:268969/150‑1 (MQ=255)
                                                                                                                                 tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                   <  1:2430774/150‑1 (MQ=255)
                                                                                                                                    aTTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGAt                <  2:817489/150‑1 (MQ=255)
                                                                                                                                        cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg            >  2:1903840/1‑150 (MQ=255)
                                                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt           <  2:2813434/150‑1 (MQ=255)
                                                                                                                                         aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt           <  1:2666020/150‑1 (MQ=255)
                                                                                                                                          aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          <  2:1227839/150‑1 (MQ=255)
                                                                                                                                          aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGGAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg          >  1:409442/1‑150 (MQ=255)
                                                                                                                                            atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa        <  1:2904234/150‑1 (MQ=255)
                                                                                                                                              ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  2:94320/150‑1 (MQ=255)
                                                                                                                                              ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:353390/150‑1 (MQ=255)
                                                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:2985033/150‑1 (MQ=255)
                                                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  2:704985/150‑1 (MQ=255)
                                                                                                                                              ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa      <  1:2794851/150‑1 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  1:1814849/1‑150 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:1746544/1‑150 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:1166509/1‑150 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  2:105003/1‑150 (MQ=255)
                                                                                                                                                  tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa  >  1:317554/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
TCTACCACCACTTGCATCGTTTCGAGATTGTGAATATTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATA  >  NC_000913/2177098‑2177393

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: