Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,279 C→A 9.3% intergenic (‑75/+233) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2790CA 9.3% 449.5 / 6.1 164intergenic (‑75/+233)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base C (66/76);  new base A (18/3);  total (85/79)
Fisher's exact test for biased strand distribution p-value = 7.96e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 164 total aligned reads displayed.

TTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTT  >  NC_000913/2177134‑2177427
                                                                                                                                                 |                                                                                                                                                    
 tGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaa                                                                                                                                                 >  2:2328061/1‑150 (MQ=255)
   aaagCCGCAACCGCATAACCGCCGCGCAGTGCGAAGAACACCATCTGCATTCACGATACCACCAACAATTTCATATCCTCTCGATAGATTTCGAATTCAAAATATAATCAAATTATTTCTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                               <  2:1668197/147‑1 (MQ=255)
   aaTGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACaaaaa                                                                                                                                               <  1:1144225/150‑1 (MQ=255)
     tGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACaaaaaag                                                                                                                                             >  1:1044046/1‑148 (MQ=255)
       ccGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGGTTGCGGGTTCAAAATATAATGAAAGAATTTTTTTTTAAATATCGCGATAACCAGCACCAAAAcccc                                                                                                                                           >  1:650409/1‑147 (MQ=255)
        cGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGTAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACa                                                                                                                                          >  2:522759/1‑150 (MQ=255)
           aaCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTGGATTTGAAAATATAAAGAAATTATTTTTTTTTTAATATAGTGATAAAGATCACAAAAAACACAata                                                                                                                                       >  1:89627/1‑150 (MQ=255)
            aCCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAATTTAATTATTTGTTTTTATATATAGAGATAAAGATCACAAAAACCACAatat                                                                                                                                      >  1:2344083/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGACATAACGATCACAAAAAAGACAATATGaaa                                                                                                                                  >  2:3134612/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTaaa                                                                                                                                  >  2:431199/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAAAATGaaa                                                                                                                                  >  1:1708198/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTGAAAATATAATTTAATTATTTGTTTTTAAATATAGAGATAAAGATCACCAAAACGACAATATTaaa                                                                                                                                  >  1:486498/1‑150 (MQ=255)
                cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTATTTGTTTTTAAAAATCGAGATAAAGATAACAAAAAAGACAAAATTaaa                                                                                                                                  >  1:1503236/1‑150 (MQ=255)
                    aCCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAatta                                                                                                                              >  2:3166962/1‑150 (MQ=255)
                     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                             <  2:1088746/150‑1 (MQ=255)
                     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                             <  2:610783/150‑1 (MQ=255)
                       gccgcGCTGTGCGTTGTTCTGCATCTGCTTTGTGGATACCACGTACATTTTCATTTCCTGTGGTTTGTTTTCGTTTTCAAATTATTCTGAAATTTTTTGTTTTTAATTATCTTGATAAGTATCACAAAAACGACAATATGTAAATTTTTc                                                                                                                           <  2:362020/150‑1 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                                                           <  2:904029/150‑1 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGTTTTCAAAATATAAATAAAATATTTTTTTTTTAATATAGTGATAACGATAACAAAAACGACACAATTAAAattttta                                                                                                                           >  1:3037694/1‑149 (MQ=255)
                       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTGAAAATATAAATTAAATATTTTTTTTTTAAAATGGAGCTAAAGAACGAAAAAAACACAAAATTTAAaatattt                                                                                                                           >  1:1428292/1‑149 (MQ=255)
                         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATTAAAATTATTCgg                                                                                                                         >  2:353390/1‑149 (MQ=255)
                             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTTAAAATAGAGATAAAGATCAAAAAAAAGACAATATTAAAATTATTAgaggag                                                                                                                     >  1:456635/1‑150 (MQ=255)
                             cTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                                                                     <  2:857005/150‑1 (MQ=255)
                                tgCGTTGTTCAGCATCTGCTTTGTCGATACCCCGAACATTTTCATCTCCTGTGTTTTTTTTTCTATTACAAAATAAAATTAATTTTTTTTTTTTTAATTATCTAGATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGa                                                                                                                  <  1:1920301/150‑1 (MQ=255)
                                  cttttcccAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                <  2:3051828/143‑1 (MQ=255)
                                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                <  2:1036794/150‑1 (MQ=255)
                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAAAATTAAAATTAATGGAGGGGTGAAAg                                                                                                               >  1:778464/1‑150 (MQ=255)
                                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGATATAAACATCACAAAAAAGAAAATATTAAAATAATAAGATGAGTTAAAg                                                                                                               >  1:1804821/1‑150 (MQ=255)
                                   gttgttCAGCATCTGCTTTGCCGACCCCACTTACTTTTAAATATCCTGTCTTTTGTTTTCGATTTCAAAATATATTTAATTTTTTTTTTTTTAAATATCGATAAAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAg                                                                                                               <  1:2312300/150‑1 (MQ=255)
                                     tgttCAGCATCTGCTTTTTCGATACCACTTACATTTTCATATCCTTTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                                                             <  2:2595131/150‑1 (MQ=255)
                                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAAAACAAAAAAGACAATATTAAAATTATTATAGGAGTGAAAGGcca                                                                                                           >  2:2807672/1‑148 (MQ=255)
                                        tCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAAAATCGAGATAACGATCAAAAAAAAGACAATATGAAAATTATTAGAGGCGTGGAAGGCaaa                                                                                                          >  2:2826417/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGTATTAAAAATATAAATTAAAAATTTTTTTTTATATATAAATATAAACATAAAAAAAAAACAAATAATAAAATTATTAGAAGATTGGAATGGaaaaaa                                                                                                       >  1:2619285/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAAAAATTAAAATATATTATTTTTAATATATATATAAACAAAAAAAAAAAGACAAAAATATAAATATTAGAGGGGTGTAAAGCagaaaa                                                                                                       >  1:2486379/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAATATAAATAAATTATTTTTTTTTTAAAATAGAGATAAAAATAAAAAAAAAGACAAAATTAAAAATATTATAGGGGTGTAAGGGaaaaaa                                                                                                       >  1:1676666/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAAATAAAATATTTTTTTTTTAATAAAGAGATAACAATAACAAAAAACAAAAAAATAAAATTATAAGATGAGTGTAATGCcaaaaa                                                                                                       >  1:1374782/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAAATAAATAAAATTTTTTTTTTTTAATAACAATATAAAGATAACAAAAAACAAAAAATTAAAAATATTATAAGAGTGGAATGCaaaaaa                                                                                                       >  1:2619183/1‑150 (MQ=255)
                                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTTAAAATATAGATAAACATAAAAAAAAAAAAAAAAATAAAAAAAATAGAGGGGGGTAAGGCaaaaaa                                                                                                       >  1:17256/1‑150 (MQ=255)
                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                      <  2:1737590/150‑1 (MQ=255)
                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                      <  2:2668505/150‑1 (MQ=255)
                                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAACGCaaaaaaa                                                                                                      >  2:2936595/1‑150 (MQ=255)
                                             aTCGGCTTTGTCGATACCACGTACATTTTCAGATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                     <  2:2288245/150‑1 (MQ=255)
                                              tCTGCTTTGTCTATACCACGTACTTTTTCATATCATTTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACg                                                                                                    <  2:1836536/150‑1 (MQ=255)
                                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAAAATAAATAAAATATTTTTTTTTTAATATAGAGATAAAGAAAACAAAAAAGACAAAAAGAAAAATATTAGATGGGTGGAAGGCcaaaaaaaag                                                                                                   >  1:2767602/1‑147 (MQ=255)
                                                 gCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATAGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTAGAGGTGTGTAAGGCAAAAAAACGGcc                                                                                                 >  2:3046331/1‑150 (MQ=255)
                                                     tGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAAAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                             <  2:1369806/150‑1 (MQ=255)
                                                     tGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                             <  2:1543387/150‑1 (MQ=255)
                                                       tCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGa                                                                                           <  2:816901/150‑1 (MQ=255)
                                                         taTACCACTTAATTTTTAATTTCTTTTTTTTTTTTTTCTATTTAAAAATATATTTAATTTATTTTTTTTTAATTTTCTATATAACTAAAAAAAAAACGAAAATATTAAATTTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATa                                                                                         <  1:2219702/149‑1 (MQ=255)
                                                          aTACCACTTAATTTTTAATATCTTGTTTTTTTTTTTCTATTTAAAAATATAATTAAATTTTTTTTTTTTAAATATCTAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAg                                                                                        <  1:3221643/150‑1 (MQ=255)
                                                               aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                   >  2:488396/1‑150 (MQ=255)
                                                                  tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGAGAGGGAAGCCg                                                                                >  2:1954964/1‑150 (MQ=255)
                                                                    aaTTTTCTTATCCTGTCTTTTTTTTTCTATTACAAAATAAAATGAAATTATTTTTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                              <  1:2045937/149‑1 (MQ=255)
                                                                    aaTTTACATATCCTTTCTTTTTTTTTCTATAAAAAAATATAATAAAATTATTTTTTTAAAAATATCCACAAAACGAACACAAAAACGACAATATTAAAATTATTCGAGGAGTTAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTa                                                                              <  1:1765132/149‑1 (MQ=255)
                                                                         taatatCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                         <  1:2397653/148‑1 (MQ=255)
                                                                            tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGCGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                                                      >  2:1343971/1‑150 (MQ=255)
                                                                             ttaatttCGTTTGTTTTCGTTTTCTATATATACTGAATTTTTTTGTTTTTATTTTTCGAGATAAGGATAAGATAATCGACAATTTGAAAATTATTCGTGGAGTGAAAGGCATAAAAACGGCCTCCCGATAGGGAAGCCGTAGCTAAGTgc                                                                     <  2:904001/144‑1 (MQ=255)
                                                                                 tGTCGTTTGTTTTCGATTTCAAAAAATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCgt                                                                 >  2:3037123/1‑150 (MQ=255)
                                                                                     gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                             >  1:2987692/1‑150 (MQ=255)
                                                                                       ttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttttt                                                           >  2:2556519/1‑150 (MQ=255)
                                                                                        tgtttTCTATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTa                                                          <  2:2725905/150‑1 (MQ=255)
                                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                        <  2:1345360/150‑1 (MQ=255)
                                                                                           tttCGTTTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                       <  1:612346/150‑1 (MQ=255)
                                                                                            ttagATTTCAAAATATAATGACATTATTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGTGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                      <  1:1875513/147‑1 (MQ=255)
                                                                                             tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                     <  1:2430774/150‑1 (MQ=255)
                                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                    <  1:3166962/150‑1 (MQ=255)
                                                                                               gATTTCAAAATATAATGAAACTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGACAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCACAGTGCGCGTGTGTTTGTGCCGGa                                                   >  1:858267/1‑150 (MQ=255)
                                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                              >  2:1903840/1‑150 (MQ=255)
                                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                             <  2:2813434/150‑1 (MQ=255)
                                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                             >  1:1246284/1‑150 (MQ=255)
                                                                                                      aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                            <  2:1227839/150‑1 (MQ=255)
                                                                                                      aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                            >  1:2198698/1‑150 (MQ=255)
                                                                                                       aaTATAATGAAATTATTTGTTTTTAAATATCTAGATACCTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                           <  1:1392005/150‑1 (MQ=255)
                                                                                                        atatAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTa                                          <  1:2904234/150‑1 (MQ=255)
                                                                                                          ataATGATATTATTTGTTTTTAAATATCGAGTTAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  1:2985070/150‑1 (MQ=255)
                                                                                                          ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  2:94320/150‑1 (MQ=255)
                                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  1:2985033/150‑1 (MQ=255)
                                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  2:704985/150‑1 (MQ=255)
                                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  1:2794851/150‑1 (MQ=255)
                                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                        <  1:368314/150‑1 (MQ=255)
                                                                                                            aattaaattaTTTGTTTTTAAATATCGATATAACGAACACAAAAACGACAATATTAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACa                                      <  1:1665877/150‑1 (MQ=255)
                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                    >  2:1166509/1‑150 (MQ=255)
                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                    >  2:105039/1‑150 (MQ=255)
                                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGGAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTGAACATa                                    >  2:3254131/1‑150 (MQ=255)
                                                                                                                      tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                            >  2:1442322/1‑150 (MQ=255)
                                                                                                                        tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                          >  1:1295001/1‑150 (MQ=255)
                                                                                                                        tGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTg                          >  1:60333/1‑150 (MQ=255)
                                                                                                                          tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGAGGCGGGGTAATCTTACGTGGCAGTGCt                        >  1:2475485/1‑150 (MQ=255)
                                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                       >  2:1957659/1‑150 (MQ=255)
                                                                                                                             ttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATg                     >  1:2091987/1‑150 (MQ=255)
                                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTCTGTa                   >  2:644788/1‑150 (MQ=255)
                                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                   >  2:816979/1‑150 (MQ=255)
                                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                   <  2:2466475/150‑1 (MQ=255)
                                                                                                                                        gaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATa          <  2:2588859/150‑1 (MQ=255)
                                                                                                                                           aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa       <  1:3205679/150‑1 (MQ=255)
                                                                                                                                           aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa       <  2:1789217/150‑1 (MQ=255)
                                                                                                                                             cGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACg     <  1:2631484/150‑1 (MQ=255)
                                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc    >  2:1530028/1‑150 (MQ=255)
                                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc    >  1:1464881/1‑150 (MQ=255)
                                                                                                                                                tCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCtt  <  2:17256/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
TTGAATGCCGGAACCGCATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTT  >  NC_000913/2177134‑2177427

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: