Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,177,296 G→T 9.4% intergenic (‑92/+216) gatY ← / ← fbaB D‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,177,2960GT 9.4% 571.0 / 2.6 171intergenic (‑92/+216)gatY/fbaBD‑tagatose 1,6‑bisphosphate aldolase 2, catalytic subunit/fructose‑bisphosphate aldolase class I
Reads supporting (aligned to +/- strand):  ref base G (68/80);  new base T (20/3);  total (88/83)
Fisher's exact test for biased strand distribution p-value = 2.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 171 total aligned reads displayed.

CATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGC  >  NC_000913/2177150‑2177443
                                                                                                                                                  |                                                                                                                                                   
cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAATATAAAGAAATTATTTTTTTTTAAATATAGACATAACGATCACAAAAAAGACAATATGaaa                                                                                                                                                  >  2:3134612/1‑150 (MQ=255)
cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTCAAAATATAAAGAAATTATTTGTTTTTAAATATAGAGATAAAGATCACAAAAACGACAATATTaaa                                                                                                                                                  >  2:431199/1‑150 (MQ=255)
cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCAACAAAACGACAAAATGaaa                                                                                                                                                  >  2:2292569/1‑150 (MQ=255)
cATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAATAAATAAATTATTTGTTTTTAAAAATCGAGATAAAGATAACAAAAAAGACAAAATTaaa                                                                                                                                                  >  1:1503236/1‑150 (MQ=255)
     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                                             <  2:325215/150‑1 (MQ=255)
     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAattat                                                                                                                                             <  2:610783/150‑1 (MQ=255)
     ccgccgCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAAAATAAAGTAAATATTTTTTTTTTAATATAGAGATAAAGAAAAAAAAAAAAACAAAATGAAAaatat                                                                                                                                             >  1:1167895/1‑150 (MQ=255)
       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTc                                                                                                                                           <  2:904029/150‑1 (MQ=255)
       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGGTTTAGTTTTCAAAATATAAATAAAATATTTTTTTTTTAATATAGTGATAACGATAACAAAAACGACACAATTAAAattttta                                                                                                                                           >  1:3037694/1‑149 (MQ=255)
       gccgcGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGTTTTGAAAATATAAATTAAATATTTTTTTTTTAAAATGGAGCTAAAGAACGAAAAAAACACAAAATTTAAaatattt                                                                                                                                           >  1:1428292/1‑149 (MQ=255)
         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCga                                                                                                                                         <  2:351064/150‑1 (MQ=255)
         cgcgCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAAAGATCACAAAAACGACAAAATTAAAATTATTCgg                                                                                                                                         >  2:353390/1‑149 (MQ=255)
             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAATAAATAAATTATTTTTTTTTTAAAATAGAGATAAAGATCAAAAAAAAGACAATATTAAAATTATTAgaggag                                                                                                                                     >  1:456635/1‑150 (MQ=255)
             cTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTAAAAATATAATTGAATTATTTTTTTTTTAATATAGTGAGAAAGATGACAAAAAAAACAAAAATAAAATTATTAgaggtg                                                                                                                                     >  1:1500129/1‑148 (MQ=255)
             cTGTGCGTTGTTCAGAATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCgaggag                                                                                                                                     <  2:857005/150‑1 (MQ=255)
                tgCGTTGTTCAGCATCTGCTTTGTCGATACCCCGAACATTTTCATCTCCTGTGTTTTTTTTTCTATTACAAAATAAAATTAATTTTTTTTTTTTTAATTATCTAGATAACTAACAAAAACACGACAATATGAAAATTATTCGAGGAGTGa                                                                                                                                  <  1:1920301/150‑1 (MQ=255)
                  cttttcccAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                                <  2:3051828/143‑1 (MQ=255)
                  cGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                                <  2:1036794/150‑1 (MQ=255)
                  cGTTGTACCGCACCTGCTTTGACGATACCCCGTACATTTTCATATCCTGTCTTTTGTTTTCGATTAAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGaaa                                                                                                                                <  2:3051811/150‑1 (MQ=255)
                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAATATAATTAAAATATTTGTTTTTTAATATCGAGATAAAGATAACAAAAAAGACAAAATTAAAATTAATGGAGGGGTGAAAg                                                                                                                               >  1:778464/1‑150 (MQ=255)
                   gttgttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTAGATTTAAAAAAATAATGAAATTATTTTTTTTTAAATATAGATATAAACATCACAAAAAAGAAAATATTAAAATAATAAGATGAGTTAAAg                                                                                                                               >  1:1804821/1‑150 (MQ=255)
                     tgttCAGCATCTGCTTTTTCGATACCACTTACATTTTCATATCCTTTCGTTTTTTTTCTATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGc                                                                                                                             <  2:2595131/150‑1 (MQ=255)
                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTCAAAAAATAATGAAATTATTTTTTTTTAAATATAGAGATAAACATCACAAAAAAGACAAAATGAAAATTATTAGAGGGGTGTAATGCaa                                                                                                                           >  2:684122/1‑150 (MQ=255)
                       ttCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGAAAACAAAAAAGACAATATTAAAATTATTATAGGAGTGAAAGGcca                                                                                                                           >  2:2807672/1‑148 (MQ=255)
                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTAAAAAAAGAGATAAAGCAAAAAAAAAAAAAAAAATTAAAATTATTAGAGGGGGGTAAGGCaaaaaa                                                                                                                       >  1:1676585/1‑150 (MQ=255)
                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTTTTTTAGATTTAAAAAAATAAATAAAATATTTTTTTTTTAATAAAGAGATAACAATAACAAAAAACAAAAAAATAAAATTATAAGATGAGTGTAATGCcaaaaa                                                                                                                       >  1:1374782/1‑150 (MQ=255)
                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTGGATTTGAAAAAATAAATAAAATTTTTTTTTTTTAATAACAATATAAAGATAACAAAAAACAAAAAATTAAAAATATTATAAGAGTGGAATGCaaaaaa                                                                                                                       >  1:2619183/1‑150 (MQ=255)
                           gCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTAGATTTAAAAAAAAAAATAAAATATTTTTTTTTTAAAATATAGATAAACATAAAAAAAAAAAAAAAAATAAAAAAAATAGAGGGGGGTAAGGCaaaaaa                                                                                                                       >  1:17256/1‑150 (MQ=255)
                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                                      <  2:2668505/150‑1 (MQ=255)
                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCaaaaaaa                                                                                                                      <  2:1737590/150‑1 (MQ=255)
                            cATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAAAGATCACAAAAAAGACAATATGAAAATTATTCGAGGAGTGAAACGCaaaaaaa                                                                                                                      >  2:2936595/1‑150 (MQ=255)
                             aTCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTAGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATAGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAAc                                                                                                                     >  2:2118686/1‑150 (MQ=255)
                               cTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTGTGTTTTTGATTTGAAAAAATAAATAAAATATTTTTTTTTTAATATAGAGATAAAGAAAACAAAAAAGACAAAAAGAAAAATATTAGATGGGTGGAAGGCcaaaaaaaag                                                                                                                   >  1:2767602/1‑147 (MQ=255)
                                     tGTCTATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCTATTTAAAAATAAAATGAAATTATTTGTTTTTAAATATCGATATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                                             <  2:1369806/150‑1 (MQ=255)
                                     tGTCGATACCACGTACATTTTCATATCCTGTCGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTccc                                                                                                             <  2:1543387/150‑1 (MQ=255)
                                         gATACCACGTACATTTTCATTTCCTGTCTTTTGTTTTCTATTTCAAAATATATTTAAATTATTTTTTTTTAAATATCTAGATAACGATCACAAAAACTACAATATGAAAATTATTCGAGGTGTGAAAGGCAAAAAAACGGCCTCCCGATa                                                                                                         <  1:2268153/150‑1 (MQ=255)
                                               aCGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTAGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                                   >  2:488396/1‑150 (MQ=255)
                                               aCGTACATTTTCATATCCTGTCGTGTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAg                                                                                                   >  2:2831113/1‑150 (MQ=255)
                                                  tACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATAACAAAAACGACAATATGTAAATTATTCGAGGAGTGAAAGGCGAAAAAACGGCCTCCCGAGAGGGAAGCCg                                                                                                >  2:1954964/1‑150 (MQ=255)
                                                         taatatCCTGTCGTTTTTTTTCTATTTCAAAATATAATTAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCaaa                                                                                         <  1:2397653/148‑1 (MQ=255)
                                                            tatCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAAAATGAAAATTATTCGAGGCGTGAAAGGCAAAAAAAAGGCCTCCCGATAGGGAAGCCGTAGCAAAGTg                                                                                      >  2:1343971/1‑150 (MQ=255)
                                                               ccTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTgcgc                                                                                   <  2:89627/150‑1 (MQ=255)
                                                                    cGTTTTTTTTCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGtt                                                                              <  2:2560593/150‑1 (MQ=255)
                                                                     gtttgtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGttt                                                                             >  1:2987642/1‑150 (MQ=255)
                                                                         gtttTCTATTTCAAAATATAATTAAATTATTTTTTTTTAAATATCTAGTTAACGATAACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                                         <  2:1104105/150‑1 (MQ=255)
                                                                         gtttTCGATTTCAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                                         <  1:215584/150‑1 (MQ=255)
                                                                         gtttTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTAt                                                                         <  2:1896407/150‑1 (MQ=255)
                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                                        >  1:1708010/1‑150 (MQ=255)
                                                                          ttttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATg                                                                        <  2:1345360/150‑1 (MQ=255)
                                                                           tttCGTTTTAAAAATATAATCAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                                       <  1:612346/150‑1 (MQ=255)
                                                                           tttCGATTTAAAAATATAATGAAATTATTTTTTTTTAAATATCGAGATAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGc                                                                       <  1:612094/150‑1 (MQ=255)
                                                                            ttagATTTCAAAATATAATGACATTATTTTTTTTTAAATATCGAGATAACTATCACAAACACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGTGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                                      <  1:1875513/147‑1 (MQ=255)
                                                                            ttCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGcc                                                                      <  1:174211/150‑1 (MQ=255)
                                                                             tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                                     <  2:268969/150‑1 (MQ=255)
                                                                             tCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCg                                                                     <  1:2430774/150‑1 (MQ=255)
                                                                              cGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCgg                                                                    <  1:3166962/150‑1 (MQ=255)
                                                                                    cAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCgg                                                              >  2:1903840/1‑150 (MQ=255)
                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAACAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                                             <  2:2813434/150‑1 (MQ=255)
                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                                             >  1:1246284/1‑150 (MQ=255)
                                                                                     aaaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgt                                                             <  1:2666020/150‑1 (MQ=255)
                                                                                      aaaTATAATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                                            <  2:1227839/150‑1 (MQ=255)
                                                                                      aaaTATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtg                                                            >  1:2198698/1‑150 (MQ=255)
                                                                                       aaTATAATGAAATTATTTGTTTTTAAATATCTAGATACCTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGgtgt                                                           <  1:1392005/150‑1 (MQ=255)
                                                                                          ataATGATATTATTTGTTTTTAAATATCGAGTTAACTATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  1:2985070/150‑1 (MQ=255)
                                                                                          ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  2:94320/150‑1 (MQ=255)
                                                                                          ataATGAAATTATTTTTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  1:353390/150‑1 (MQ=255)
                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  1:28451/150‑1 (MQ=255)
                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  2:704985/150‑1 (MQ=255)
                                                                                          ataATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTaaa                                                        <  1:368314/150‑1 (MQ=255)
                                                                                              tGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATa                                                    >  2:105039/1‑150 (MQ=255)
                                                                                                aaaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACg                                                  <  2:298122/150‑1 (MQ=255)
                                                                                                 aaTTATTTGTTTTTAAATATCGAGATAACGATCACAAAATCGACAATATGTAAATTATTCGAGGAGTGAAAGGCAAATAAACGGCCTCCCGTTAGGGAAGCCGTAGCTAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGt                                                 >  2:709470/1‑150 (MQ=255)
                                                                                                   ttattTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTgg                                               <  1:2328061/150‑1 (MQ=255)
                                                                                                      tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                                            >  2:1378435/1‑150 (MQ=255)
                                                                                                      tttGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAg                                            >  2:1442322/1‑150 (MQ=255)
                                                                                                          tttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGACGCCGTAGCACAGTGCGCGTGTTTTTATGCCGGAGGCGGGGTAATCTTACGTGGCAGTGCt                                        >  1:2475485/1‑150 (MQ=255)
                                                                                                           ttttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTa                                       >  1:2626114/1‑150 (MQ=255)
                                                                                                            tttAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTAt                                      >  1:901005/1‑150 (MQ=255)
                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                                   >  2:816979/1‑150 (MQ=255)
                                                                                                               aaaTATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTa                                   <  2:2466475/150‑1 (MQ=255)
                                                                                                                      gagaTAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGa                            <  1:504632/150‑1 (MQ=255)
                                                                                                                           aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa                       <  1:3205679/150‑1 (MQ=255)
                                                                                                                           aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa                       <  1:2500326/150‑1 (MQ=255)
                                                                                                                           aaCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGa                       <  2:1789217/150‑1 (MQ=255)
                                                                                                                            aCGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGAc                      <  1:2937076/150‑1 (MQ=255)
                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACga                    >  1:2994141/1‑149 (MQ=255)
                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc                    >  1:2070739/1‑150 (MQ=255)
                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc                    >  2:1530028/1‑150 (MQ=255)
                                                                                                                              gATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGc                    >  1:1464881/1‑150 (MQ=255)
                                                                                                                                tCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCtt                  <  2:17256/150‑1 (MQ=255)
                                                                                                                                  acaAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCa                >  2:1655853/1‑150 (MQ=255)
                                                                                                                                    aaaaaCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGc              >  2:365783/1‑150 (MQ=255)
                                                                                                                                    aaaaaCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGc              <  2:1171215/150‑1 (MQ=255)
                                                                                                                                    aaaaaCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGc              <  1:1867742/150‑1 (MQ=255)
                                                                                                                                    aaaaaCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGc              >  2:1713071/1‑150 (MQ=255)
                                                                                                                                       aaCGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTc           <  2:3037694/150‑1 (MQ=255)
                                                                                                                                              atatGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAg    >  2:2736553/1‑150 (MQ=255)
                                                                                                                                              atatGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAg    >  2:2736522/1‑150 (MQ=255)
                                                                                                                                              atatGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAg    >  2:1927453/1‑150 (MQ=255)
                                                                                                                                              atatGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAg    >  2:1927343/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
CATAACCGCCGCGCTGTGCGTTGTTCAGCATCTGCTTTGTCGATACCACGTACATTTTCATATCCTGTCGTTTGTTTTCGATTTCAAAATATAATGAAATTATTTGTTTTTAAATATCGAGATAACGATCACAAAAACGACAATATGAAAATTATTCGAGGAGTGAAAGGCAAAAAAACGGCCTCCCGATAGGGAAGCCGTAGCAAAGTGCGCGTGTTTTTATGCCGGATGCGGTGTAAACATACGTGGCAGTGCTATGTAGGCATGATAAGACGCTTCAGCGTCGCATCAGGC  >  NC_000913/2177150‑2177443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: