Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,655,051 T→A 5.2% intergenic (‑118/+24) sseB ← / ← pepB rhodanase‑like enzyme, sulfur transfer from thiosulfate/aminopeptidase B

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,655,0510TA 5.2% 698.5 / 19.4 194intergenic (‑118/+24)sseB/pepBrhodanase‑like enzyme, sulfur transfer from thiosulfate/aminopeptidase B
Reads supporting (aligned to +/- strand):  ref base T (99/85);  new base A (8/2);  total (107/87)
Fisher's exact test for biased strand distribution p-value = 1.90e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 203 total aligned reads displayed.

AGGTCTTCAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTT  >  NC_000913/2654902‑2655200
                                                                                                                                                     |                                                                                                                                                     
agGTCTTCAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGtt                                                                                                                                                       <  2:590652/150‑1 (MQ=255)
     ttCAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                  >  2:2062678/1‑150 (MQ=255)
     ttCAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTAcgc                                                                                                                                                  <  2:1606438/150‑1 (MQ=255)
      tCAAGTTCGTTTTTTGTTTCGGACATAAGAAACACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTTGGCCGGATAAGGCGTTTAcgcc                                                                                                                                                 <  1:2229307/150‑1 (MQ=255)
       cAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTAcgccg                                                                                                                                                >  2:3207957/1‑150 (MQ=255)
           ttCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                            >  2:2336668/1‑150 (MQ=255)
           ttCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                            >  1:1718229/1‑150 (MQ=255)
           ttCGTTTTGTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                                                                                                                                            <  1:2008065/150‑1 (MQ=255)
              gTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                         >  1:1162609/1‑150 (MQ=255)
              gTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                                                                                                                                         <  2:2857018/150‑1 (MQ=255)
               ttttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATGCGGCGTAAACGCCTTATCCGGc                                                                                                                                        <  1:3249241/150‑1 (MQ=37)
               ttttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                        >  2:1172405/1‑150 (MQ=255)
               tattttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc                                                                                                                                        >  2:2988460/3‑150 (MQ=255)
                tttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                       <  1:1587458/150‑1 (MQ=255)
                tttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCa                                                                                                                                       <  1:679620/150‑1 (MQ=255)
                 ttttGTTTCGGACATAAGAACCTCAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCat                                                                                                                                      >  2:1895212/1‑150 (MQ=255)
                 ttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCat                                                                                                                                      >  2:2462678/1‑150 (MQ=255)
                 ttttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCat                                                                                                                                      >  1:1270716/1‑150 (MQ=255)
                  tttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCata                                                                                                                                     >  2:78029/1‑150 (MQ=255)
                   ttGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCatat                                                                                                                                    <  2:1822839/150‑1 (MQ=255)
                        tCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGtt                                                                                                                               >  1:1579780/1‑150 (MQ=255)
                               tAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCg                                                                                                                        >  1:343634/1‑150 (MQ=255)
                               tAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCg                                                                                                                        >  2:1788688/1‑150 (MQ=255)
                                aaGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGt                                                                                                                       >  1:270976/1‑150 (MQ=255)
                                aaGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGt                                                                                                                       >  1:271153/1‑150 (MQ=255)
                                    aCCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAAc                                                                                                                   <  1:2487772/150‑1 (MQ=255)
                                      cacaATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAg                                                                                                                 >  1:2324214/1‑150 (MQ=255)
                                      cacaATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAg                                                                                                                 >  1:2912206/1‑150 (MQ=255)
                                       acaATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGa                                                                                                                >  2:2977414/1‑150 (MQ=255)
                                       acaATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGa                                                                                                                >  2:2167109/1‑150 (MQ=255)
                                           ttcattcaACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAg                                                                                                            >  2:2096106/1‑150 (MQ=255)
                                           ttcattcaACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAg                                                                                                            >  1:943884/1‑150 (MQ=255)
                                              attcaACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTa                                                                                                         <  2:1232342/150‑1 (MQ=255)
                                                  aaCGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGt                                                                                                     >  1:570190/1‑150 (MQ=255)
                                                     gTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTgcg                                                                                                  <  2:1958257/150‑1 (MQ=255)
                                                     gTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTgcg                                                                                                  <  1:2165618/150‑1 (MQ=255)
                                                             cgcAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCaa                                                                                          <  1:28066/150‑1 (MQ=255)
                                                             cgcAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCaa                                                                                          <  1:782822/150‑1 (MQ=255)
                                                             cgcAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCaa                                                                                          <  1:3055548/150‑1 (MQ=255)
                                                                   tGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCg                                                                                    <  1:677945/150‑1 (MQ=255)
                                                                     ccTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTa                                                                                  <  1:2058700/150‑1 (MQ=255)
                                                                          tGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGcc                                                                             <  1:2462678/150‑1 (MQ=255)
                                                                          tGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGcc                                                                             <  1:1895212/150‑1 (MQ=255)
                                                                           gcgcTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGccc                                                                            <  2:2629029/150‑1 (MQ=255)
                                                                             gcTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGATTTTGTAGGCCGGTTAAGGCGTTTACGCCGCATCCGGCATATTTGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGc                                                                          <  1:1071179/150‑1 (MQ=255)
                                                                               tACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAg                                                                        >  2:2407125/1‑150 (MQ=255)
                                                                                 cGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGAc                                                                      >  2:273115/1‑150 (MQ=255)
                                                                                 cGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGAc                                                                      <  2:1151413/150‑1 (MQ=255)
                                                                                   cTTATCAGGCCTACAAGGGATTCGCAATGTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCa                                                                    >  2:2351923/1‑150 (MQ=255)
                                                                                          ggCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCa                                                             >  2:2545812/1‑150 (MQ=255)
                                                                                           gCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCaa                                                            >  2:527594/1‑150 (MQ=255)
                                                                                              tACAAGGGATTCGCAATTTGTTGGATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCg                                                         >  1:5063/1‑150 (MQ=255)
                                                                                               aCAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCgg                                                        >  1:3017536/1‑150 (MQ=255)
                                                                                                 aaGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGgcg                                                      >  2:2290863/1‑150 (MQ=255)
                                                                                                 aaGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGgcg                                                      >  2:579111/1‑150 (MQ=255)
                                                                                                 aaGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGgcg                                                      >  2:317912/1‑150 (MQ=255)
                                                                                                    ggATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCtt                                                   >  2:348866/1‑150 (MQ=255)
                                                                                                      aTTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTa                                                 >  1:3074284/1‑150 (MQ=255)
                                                                                                      aTTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTa                                                 >  1:1314584/1‑150 (MQ=255)
                                                                                                      aTTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTa                                                 <  2:3135177/150‑1 (MQ=255)
                                                                                                          gCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGt                                             >  2:884812/1‑150 (MQ=255)
                                                                                                          gCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGt                                             >  2:1414172/1‑150 (MQ=255)
                                                                                                            attttGTTGAATTTGTAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAATCGGCGCTTTACGGTaa                                           <  2:1677421/148‑1 (MQ=255)
                                                                                                            aaTTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTaa                                           <  1:2413263/150‑1 (MQ=255)
                                                                                                                  ttgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGcc                                     >  1:1547213/1‑150 (MQ=255)
                                                                                                                  ttgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGcc                                     >  1:72674/1‑150 (MQ=255)
                                                                                                                  ttgAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATACTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGcc                                     <  2:2612465/150‑1 (MQ=255)
                                                                                                                    gAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGa                                   <  1:231643/150‑1 (MQ=255)
                                                                                                                     aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAg                                  >  1:1385820/1‑150 (MQ=255)
                                                                                                                     aaTTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAg                                  <  1:1397518/150‑1 (MQ=255)
                                                                                                                         tGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGt                              <  2:458695/150‑1 (MQ=255)
                                                                                                                         tGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGt                              <  1:1958018/150‑1 (MQ=255)
                                                                                                                         tGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGt                              <  1:101425/150‑1 (MQ=255)
                                                                                                                           cAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCg                            <  1:177226/150‑1 (MQ=255)
                                                                                                                            aGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGa                           <  2:1984188/150‑1 (MQ=255)
                                                                                                                              aaTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGata                         >  1:163872/1‑150 (MQ=255)
                                                                                                                               aTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGatat                        >  2:1601754/1‑150 (MQ=255)
                                                                                                                                tttGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATg                       >  2:2953483/1‑150 (MQ=255)
                                                                                                                                  tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGca                     >  2:2671168/1‑150 (MQ=255)
                                                                                                                                  tGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGca                     >  2:1133528/1‑150 (MQ=255)
                                                                                                                                   gTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATGAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcag                    <  1:1841119/150‑1 (MQ=255)
                                                                                                                                      tgCCGGATGCGGCGTAAACGCCTTATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagcca                 >  2:297002/2‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagccag                >  1:1452520/1‑150 (MQ=255)
                                                                                                                                       gCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagccag                >  2:2484738/1‑150 (MQ=255)
                                                                                                                                        ccGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGcagccagc               >  2:2543668/1‑150 (MQ=255)
                                                                                                                                        ccGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAAAAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGATTTACGGGAAGTCGCCGAGCAGTCGATATGcatccagc               >  1:2964916/1‑150 (MQ=255)
                                                                                                                                         cGGATGCGGCGTAAACGCCTTATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCagccagcc              >  1:1618796/1‑150 (MQ=255)
                                                                                                                                         cGGATGCGGCGTAAACGCCTTATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGGCGATATGCagccagcc              >  1:2538471/1‑150 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCagccagcc              <  1:2748466/150‑1 (MQ=255)
                                                                                                                                         cGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCagccagcc              <  1:462789/150‑1 (MQ=255)
                                                                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCt             >  1:606335/1‑150 (MQ=255)
                                                                                                                                          ggATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCt             <  2:1523036/150‑1 (MQ=255)
                                                                                                                                            aTAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTg           <  1:2093187/150‑1 (MQ=255)
                                                                                                                                            aTAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTg           <  2:686782/150‑1 (MQ=255)
                                                                                                                                              gcggCGTAAACGCCTTATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCt         >  1:3188327/3‑150 (MQ=255)
                                                                                                                                              aaGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCt         <  1:2303100/150‑1 (MQ=255)
                                                                                                                                              aaGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCt         <  2:1273890/150‑1 (MQ=255)
                                                                                                                                                 gCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTGTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGAt      >  1:2924734/1‑150 (MQ=255)
                                                                                                                                                     ttACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGtt  <  1:49841/150‑1 (MQ=255)
                                                                                                                                                     ttACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGtt  >  1:2895006/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
AGGTCTTCAAGTTCGTTTTTTGTTTCGGACATAAGAACCACAATTCATTCAACGTTCGGGGCGCAAATGCCTGATGCGCTACGCTTATCAGGCCTACAAGGGATTCGCAATTTGTTGAATTTGCAGAATTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATATTAGTTTACGCCGTTAACAGATTAGCTATCGTGCGCACACCAAGTCCCGTAGCGCCCGCAGACCACTGTTCAACCGGCGCTTTACGGTAAGTCGCCGAGCAGTCGATATGCAGCCAGCCTTGCTGATAGTT  >  NC_000913/2654902‑2655200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: