Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 420,942 G→T 5.3% intergenic (+32/‑44) brnQ → / → proY branched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913420,9420GT 5.3% 710.3 / 16.9 194intergenic (+32/‑44)brnQ/proYbranched‑chain amino acid transport system 2 carrier protein; LIV‑II transport system for Ile, Leu, and Val/proline‑specific permease
Reads supporting (aligned to +/- strand):  ref base G (86/96);  new base T (4/8);  total (90/104)
Fisher's exact test for biased strand distribution p-value = 3.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 196 total aligned reads displayed.

CGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACG  >  NC_000913/420794‑421091
                                                                                                                                                    |                                                                                                                                                      
cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCTCGGGGCTGc                                                                                                                                                       <  2:3117907/150‑1 (MQ=255)
cGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGc                                                                                                                                                       <  2:2170108/150‑1 (MQ=255)
    tACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATg                                                                                                                                                   >  1:1294309/1‑150 (MQ=255)
      ccGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGcc                                                                                                                                                 <  1:1560910/150‑1 (MQ=255)
         cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCg                                                                                                                                              <  2:1150554/150‑1 (MQ=255)
         cTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACAGTTGTTTTAACCACGGGGCTGCGATGCCCCg                                                                                                                                              >  2:237641/1‑150 (MQ=255)
          tGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGt                                                                                                                                             <  2:2993825/150‑1 (MQ=255)
          tGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGt                                                                                                                                             <  2:1001486/150‑1 (MQ=255)
           ggCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTg                                                                                                                                            <  1:1199077/150‑1 (MQ=255)
           ggCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTg                                                                                                                                            <  1:1179932/150‑1 (MQ=255)
            gCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTgg                                                                                                                                           >  1:412470/1‑150 (MQ=255)
            gCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTgg                                                                                                                                           >  1:3205881/1‑150 (MQ=255)
            gCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCATCGCAGCCCCGTgg                                                                                                                                           <  2:1568225/150‑1 (MQ=255)
              cGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGtt                                                                                                                                        >  2:721428/1‑150 (MQ=255)
               gAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGttt                                                                                                                                       <  1:754784/150‑1 (MQ=255)
                aaCAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGTTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTTCGATGCCCCGTGGtttt                                                                                                                                      <  2:1857234/150‑1 (MQ=255)
                aaCAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGtttt                                                                                                                                      <  1:1257112/150‑1 (MQ=255)
                    aGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAt                                                                                                                                  <  2:1292771/150‑1 (MQ=255)
                    aGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTAt                                                                                                                                  <  2:1632166/150‑1 (MQ=255)
                        cTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATtgtg                                                                                                                              <  2:1197353/150‑1 (MQ=255)
                        cTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATtgtg                                                                                                                              <  2:2263404/150‑1 (MQ=255)
                                gTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATggg                                                                                                                      >  1:1094762/1‑150 (MQ=255)
                                 ttAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                                                                                                                     <  2:269840/150‑1 (MQ=255)
                                 ttAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGt                                                                                                                     >  2:485446/1‑150 (MQ=255)
                                  tAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGtt                                                                                                                    <  1:717776/150‑1 (MQ=255)
                                   aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTa                                                                                                                   >  1:1622187/1‑150 (MQ=255)
                                   aaTGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTa                                                                                                                   >  1:2543683/1‑150 (MQ=255)
                                       ccAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGaa                                                                                                               >  1:2130328/1‑150 (MQ=255)
                                            aGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGAt                                                                                                          <  2:2817601/150‑1 (MQ=255)
                                             gtggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGACTTGATg                                                                                                         >  1:2770633/1‑150 (MQ=255)
                                               ggtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGa                                                                                                       <  1:1921932/150‑1 (MQ=255)
                                                gtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATTGaa                                                                                                      <  2:2116358/150‑1 (MQ=255)
                                                gtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGaa                                                                                                      <  2:2823235/150‑1 (MQ=255)
                                                gtgATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGaa                                                                                                      <  2:3036304/150‑1 (MQ=255)
                                                      gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAACTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAg                                                                                                <  1:2888753/150‑1 (MQ=255)
                                                      gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAg                                                                                                <  2:2386805/150‑1 (MQ=255)
                                                      gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAg                                                                                                <  1:2269973/150‑1 (MQ=255)
                                                      gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAg                                                                                                >  2:1336451/1‑150 (MQ=255)
                                                      gtggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAg                                                                                                <  1:450788/150‑1 (MQ=255)
                                                       tggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGa                                                                                               <  1:485446/150‑1 (MQ=255)
                                                        ggtTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGaa                                                                                              <  2:174325/150‑1 (MQ=255)
                                                          ttCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACa                                                                                            >  1:1297297/1‑150 (MQ=255)
                                                            cTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAg                                                                                          <  1:2420053/150‑1 (MQ=255)
                                                             tGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGc                                                                                         >  1:2345941/1‑150 (MQ=255)
                                                              ggCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTGTTTATTGTGTTGATGGGTTAGGACTTGATGGAAAGTAAGAACAAGCt                                                                                        >  1:1583266/1‑150 (MQ=255)
                                                               gCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTa                                                                                       >  1:1706195/1‑150 (MQ=255)
                                                                            aTCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTaa                                                                          <  2:1417258/150‑1 (MQ=255)
                                                                            aTCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTaa                                                                          <  1:876835/150‑1 (MQ=255)
                                                                                 gcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTAcc                                                                     >  1:2222103/1‑150 (MQ=255)
                                                                                 gcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTAcc                                                                     >  2:586664/1‑150 (MQ=255)
                                                                                 gcgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTATATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTAcc                                                                     >  2:1072217/1‑150 (MQ=255)
                                                                                  cgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTAccc                                                                    >  1:928856/1‑150 (MQ=255)
                                                                                           gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                           <  1:1056701/150‑1 (MQ=255)
                                                                                           gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                           >  1:2112448/1‑150 (MQ=255)
                                                                                           gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                           >  2:2317995/1‑150 (MQ=255)
                                                                                           gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                           >  2:605638/1‑150 (MQ=255)
                                                                                           gtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATAc                                                           >  1:2272368/1‑150 (MQ=255)
                                                                                             cAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGc                                                         <  1:721491/150‑1 (MQ=255)
                                                                                                gTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCttt                                                      >  2:1586584/1‑150 (MQ=255)
                                                                                                 tGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                     <  1:2073490/150‑1 (MQ=255)
                                                                                                 tGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTa                                                     <  1:2580327/150‑1 (MQ=255)
                                                                                                 tGACCTCCAGCGCTCACTAAATCACTGAACAGTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTGTTTATTGGGTTGCTGGGTTAGGAAGTGAGGGACAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCtttc                                                     >  2:2224616/1‑149 (MQ=255)
                                                                                                   aCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATg                                                   <  1:1408293/150‑1 (MQ=255)
                                                                                                   aCCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATg                                                   <  1:2213170/150‑1 (MQ=255)
                                                                                                   aCCTCCAGCGCTAACTAAATCACAGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATg                                                   >  2:2421078/1‑150 (MQ=255)
                                                                                                      tCCAGCGCTCACTAAATCACTGAACATTTGTTTTCACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCa                                                <  2:2875782/150‑1 (MQ=255)
                                                                                                       ccAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCAc                                               >  2:553578/1‑150 (MQ=255)
                                                                                                          gcgcTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTgg                                            <  1:2543300/150‑1 (MQ=255)
                                                                                                          gcgcTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTgg                                            <  1:559007/150‑1 (MQ=255)
                                                                                                              tCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTc                                        >  2:3129270/1‑150 (MQ=255)
                                                                                                               cACTAACTCACTGAACCTTTGTTTTAACCCCGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCa                                       <  2:1930860/150‑1 (MQ=255)
                                                                                                               cACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCa                                       <  1:2979794/150‑1 (MQ=255)
                                                                                                               cACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCa                                       <  1:477478/150‑1 (MQ=255)
                                                                                                                aCTAAATCACTGAACCTTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGAGGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTGTATGGCACTGGGTTCAg                                      >  2:731187/1‑150 (MQ=255)
                                                                                                                     aTCCCTGAACATTTGTTTTAACCTCGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAGCAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                                 <  2:2736501/150‑1 (MQ=255)
                                                                                                                     aTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAGCAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                                 <  2:2883105/150‑1 (MQ=255)
                                                                                                                     aTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                                 <  2:2943286/150‑1 (MQ=255)
                                                                                                                     aTCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAAtt                                 <  2:2555807/150‑1 (MQ=255)
                                                                                                                      tCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTg                                <  2:2071441/150‑1 (MQ=255)
                                                                                                                       cACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTgg                               <  1:209777/150‑1 (MQ=255)
                                                                                                                         cTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCa                             <  1:1919783/150‑1 (MQ=255)
                                                                                                                         cTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTGTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCAAAAGCGTGGGCTAAGTACCCGCCCCAGACGCGTTATGGCACAGGGTTCAGCACATGGCa                             >  1:2517968/1‑150 (MQ=255)
                                                                                                                           taaCATATGTTTTAACCACGGCGCTGCGATGCCCCGTGGTATATTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCAcc                           <  1:916468/149‑1 (MQ=255)
                                                                                                                                tttGTTTTAACCACGGGGCTTCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCt                      <  2:1147315/150‑1 (MQ=255)
                                                                                                                                tttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCt                      <  2:2501139/150‑1 (MQ=255)
                                                                                                                                tttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCt                      >  1:1822975/1‑150 (MQ=255)
                                                                                                                                tgtGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCt                      <  2:2214742/148‑1 (MQ=255)
                                                                                                                                 ttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTTTGTTGTTTGGTTAGGAATTTATGGAAAGTAAGAAAAAGCTAAAACCTGGGCTAAGTAACCCCCACAAAACCGTTATTGCACAGGGTTAAGCAATTGGCACCGGGCTg                     >  1:1479479/1‑150 (MQ=255)
                                                                                                                                 aataaaaaaCCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTg                     >  2:2788806/8‑150 (MQ=255)
                                                                                                                                      ttAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTa                <  2:907514/150‑1 (MQ=255)
                                                                                                                                       aaaCCACGGGGCATCGCAGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGTTGGTAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACAAACGCATTATGGCACGGGGTTCATCAATTGGCACCGGGCTGTTTTAc               >  1:2843081/2‑150 (MQ=255)
                                                                                                                                        aaCCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACg              <  1:2004825/150‑1 (MQ=255)
                                                                                                                                        aaCCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACg              <  1:1336451/150‑1 (MQ=255)
                                                                                                                                            aCGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATTGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTAACCGCCACATACGCTTTATGGCACGGGGTTCAGCAATTTGCACCGGGCTGTTTTACGGTTc          >  1:3111819/1‑150 (MQ=255)
                                                                                                                                            aCGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCGTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTc          >  1:2016834/1‑150 (MQ=255)
                                                                                                                                                ggCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGATAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCa      <  2:1965601/150‑1 (MQ=255)
                                                                                                                                                ggCTGCGATGCCCCGTGGTGTTTTATTTTGTTTATGGGTTAGGAATTTATGGAAAGTAAGAACAAGCAAAAACGTGGGCTAAGTAACCGCCACAAAAGCTTTATGGCACTGGGTTAAGCAAATGGCACCCGGCTGTTTTAAGGTTAGGCa      >  1:591827/1‑150 (MQ=255)
                                                                                                                                                   tGCGATGCCCCGTGGTTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGa    >  2:437775/1‑150 (MQ=255)
                                                                                                                                                   tGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGAc   >  2:2351582/1‑150 (MQ=255)
                                                                                                                                                    gCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACg  <  2:1294309/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
CGTTTACCGCTGGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTTACGGTTCGGCAGACG  >  NC_000913/420794‑421091

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: