Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,704,308 C→T 7.1% intergenic (‑245/+27) rnc ← / ← lepB RNase III/leader peptidase (signal peptidase I)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,704,3080CT 7.1% 464.4 / 17.0 144intergenic (‑245/+27)rnc/lepBRNase III/leader peptidase (signal peptidase I)
Reads supporting (aligned to +/- strand):  ref base C (63/68);  new base T (7/3);  total (70/74)
Fisher's exact test for biased strand distribution p-value = 2.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01

Only 100 of 148 total aligned reads displayed.

TATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCA  >  NC_000913/2704162‑2704457
                                                                                                                                                   |                                                                                                                                                     
tataTGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGc                                                                                                                                                    <  1:2922865/150‑1 (MQ=255)
 atatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCg                                                                                                                                                   >  2:411497/1‑150 (MQ=255)
  tatGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGa                                                                                                                                                  <  1:1038505/150‑1 (MQ=255)
   atGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGAc                                                                                                                                                 >  2:2901441/1‑150 (MQ=255)
      gggACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCGTTATGGCGACAAc                                                                                                                                              <  2:249530/150‑1 (MQ=255)
           cAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGaa                                                                                                                                         >  1:3110299/1‑150 (MQ=255)
            aaCGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGACTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTCTCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAAc                                                                                                                                        >  2:1874539/1‑150 (MQ=255)
                    ttggtgtGCGGTGGCAACCTGGAGGTGCCAACCTTAAACTTGGGGGGAATATTCTATACACAACGACGGGGGATGTCTTTAGCCACGGTAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATgg                                                                                                                                <  2:1176856/147‑1 (MQ=255)
                    ttCGTGTGCCTTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACTACGGGGGATGTCGTTAGCCACGGTAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATgg                                                                                                                                <  2:2494698/150‑1 (MQ=255)
                    ttCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATgg                                                                                                                                <  2:1242212/150‑1 (MQ=255)
                            cgttGGCAACCTGGAGTTGCCAACCTTAAACTTCGGGGGAATTTTCTATACACAACGACGGGGGATGTCTTTAGCCACGGGATATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAAt                                                                                                                        <  2:1119566/147‑1 (MQ=255)
                             cGTGGCCACCTGGAGGGGCCAACCTTAACCTTCGGGGGAATATTCTATACCCAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATg                                                                                                                       <  2:2032874/150‑1 (MQ=255)
                                             tGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                       <  2:699127/150‑1 (MQ=255)
                                              gCCAACCCTTAACCTGGGGGTTAATATTATAAACACACCGAGGGGGGTTGTCGTTCGCCACGGTATTTTTATCTAATAAATAATTCACGTTGTCGCAATAAGGGCTACAACTTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGa                                                                                                       <  1:1580158/150‑1 (MQ=255)
                                               ccAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACt                                                                                                     >  1:1840675/1‑150 (MQ=255)
                                                cAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACtt                                                                                                    >  2:1896066/1‑150 (MQ=255)
                                                 aaCCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTa                                                                                                   >  1:621448/1‑150 (MQ=255)
                                                 aaCATTAAACTTGGGGGTAATTTTCTAAACACAACGTGGGGGGATGTCGTTAGCCACGGGTTATTTATCTAATAAATAATCCACGTTGTCGCAATAAGGGCGACACCGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTa                                                                                                   <  1:1282696/150‑1 (MQ=255)
                                                  aCCTTAAACTTCGGGGTAATATTCTAAACACAACGAGGGGGGATGTCGTTAGCCAGGGGTGATTTATCTAATAAATAATTCACGTTGTCGCCATAACGGCTACAAGGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTaa                                                                                                  <  1:2415708/150‑1 (MQ=255)
                                                    ccTTAAACTTCGGGGGAATATTCTAGACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAg                                                                                                 <  2:2083173/150‑1 (MQ=255)
                                                     cTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAgc                                                                                                >  1:2222433/1‑150 (MQ=255)
                                                        aacctgggggggAATTTTATATACACAACGAGGGGGGATGTCGTTAGCCAGGGGAGATTTATCTAATAAATAATTCACGTTGTCGCATTACCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                             <  1:1647784/143‑1 (MQ=255)
                                                        aaccTTCGGGGGAATATTCTATAAACAACGAGGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                             <  1:402775/147‑1 (MQ=255)
                                                        aaaCGTCGGGGGAATATACTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATACCGGCGACACCGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCa                                                                                             <  1:3183358/150‑1 (MQ=255)
                                                           cTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAc                                                                                          <  2:518142/150‑1 (MQ=255)
                                                            ttCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGAcc                                                                                         >  1:2045009/1‑150 (MQ=255)
                                                                ggggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTc                                                                                     <  1:1721326/150‑1 (MQ=255)
                                                                 gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCg                                                                                    >  1:1837172/1‑150 (MQ=255)
                                                                 gggAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCg                                                                                    >  2:59985/1‑150 (MQ=255)
                                                                    aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGcc                                                                                 <  2:2282079/150‑1 (MQ=255)
                                                                    aaTATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGcc                                                                                 <  2:2282067/150‑1 (MQ=255)
                                                                     atatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCa                                                                                >  2:238293/1‑150 (MQ=255)
                                                                      tatTCTATACACACCGACGGGGGATGTCGTTAGCCACGGGATATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAt                                                                               <  1:413096/150‑1 (MQ=255)
                                                                      tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAt                                                                               >  1:684835/1‑150 (MQ=255)
                                                                      tatTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCAt                                                                               >  1:684740/1‑150 (MQ=255)
                                                                         tCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCg                                                                            >  1:2721921/1‑150 (MQ=255)
                                                                          cTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGc                                                                           >  2:1441331/1‑150 (MQ=255)
                                                                              acacAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCcttc                                                                       >  1:2946908/1‑150 (MQ=255)
                                                                                 caACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTg                                                                    <  2:3187670/150‑1 (MQ=255)
                                                                                  aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCTACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                                   <  1:1004146/150‑1 (MQ=255)
                                                                                  aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                                   <  2:441029/150‑1 (MQ=255)
                                                                                  aaCGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGc                                                                   <  2:264071/150‑1 (MQ=255)
                                                                                    cgacgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCtt                                                                 <  1:900413/150‑1 (MQ=255)
                                                                                       cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                              <  2:2180979/150‑1 (MQ=255)
                                                                                       cgGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATc                                                              <  1:1064391/150‑1 (MQ=255)
                                                                                         ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTTTCGCCATAACGGCGACAACGTGAACGAATATGGCTATTAATGGATGCCGCCAATGCTACTTAAGAGCAGACAAGTCGGCCATTCGCCATCTTGCTTATCGa                                                            >  1:3236940/1‑150 (MQ=255)
                                                                                         ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGa                                                            >  1:652349/1‑150 (MQ=255)
                                                                                         ggggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGa                                                            >  1:2342646/1‑150 (MQ=255)
                                                                                          gggATGTCGTTAGCCTCGGTTTATTTATCTAATATATAATTCTGGTTGTCGCATTACGGGCGACACCGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                           <  1:1463509/150‑1 (MQ=255)
                                                                                          gggATGTCGTTAGCCACGGGATATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                           <  1:1463521/150‑1 (MQ=255)
                                                                                          gggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                           <  1:2774175/150‑1 (MQ=255)
                                                                                          gggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                           <  2:1220912/150‑1 (MQ=255)
                                                                                          gggATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGaa                                                           <  2:1444909/150‑1 (MQ=255)
                                                                                                 cGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATc                                                    >  2:2681612/1‑150 (MQ=255)
                                                                                                     aGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                                >  1:1111122/1‑150 (MQ=255)
                                                                                                     aGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                                <  2:752214/150‑1 (MQ=255)
                                                                                                     aGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGa                                                >  1:1302309/1‑150 (MQ=255)
                                                                                                          cGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGcc                                           <  2:2903608/150‑1 (MQ=255)
                                                                                                           gggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCg                                          >  2:273248/1‑150 (MQ=255)
                                                                                                            ggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                         >  1:3242310/1‑150 (MQ=255)
                                                                                                            ggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                         >  2:1563573/1‑150 (MQ=255)
                                                                                                            ggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                         >  1:1665384/1‑150 (MQ=255)
                                                                                                            ggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                         >  2:469301/1‑150 (MQ=255)
                                                                                                            ggAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGt                                         >  2:2640068/1‑150 (MQ=255)
                                                                                                               gaTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGc                                      >  1:1037054/1‑150 (MQ=255)
                                                                                                                aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGca                                     <  2:1771985/150‑2 (MQ=255)
                                                                                                                aTTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCAGCCAGATAGCCGTTGcc                                     >  2:2974150/1‑150 (MQ=255)
                                                                                                                 tttATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGccc                                    <  1:586199/150‑1 (MQ=255)
                                                                                                                  atATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGAGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccg                                   <  2:1850174/149‑1 (MQ=255)
                                                                                                                     tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgacc                                <  1:1244433/150‑1 (MQ=255)
                                                                                                                     tctcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgacc                                <  1:2860985/150‑1 (MQ=255)
                                                                                                                       tcATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCccgaccga                              >  1:3087176/1‑150 (MQ=255)
                                                                                                                        catacttaatTCACGTTGTCGCCATAACGGCGACAACGTGAACGACGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                             <  2:3239329/144‑1 (MQ=255)
                                                                                                                        cATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                             <  2:856444/150‑1 (MQ=255)
                                                                                                                        cATAAATAATCCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCcgaccgac                             <  1:191119/150‑1 (MQ=255)
                                                                                                                         ataaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCgaccgacc                            <  2:1028535/150‑1 (MQ=255)
                                                                                                                          taaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                           >  1:1521521/1‑150 (MQ=255)
                                                                                                                          taaataaTTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCa                           >  1:477912/1‑150 (MQ=255)
                                                                                                                            aataatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGa                         <  1:2411262/150‑1 (MQ=255)
                                                                                                                               aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                      <  1:1874760/150‑1 (MQ=255)
                                                                                                                               aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                      <  1:2407668/150‑1 (MQ=255)
                                                                                                                               aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                      <  1:1681895/150‑1 (MQ=255)
                                                                                                                               aatTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTc                      <  1:2407684/150‑1 (MQ=255)
                                                                                                                                    aCGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTc                 <  1:3047314/150‑1 (MQ=255)
                                                                                                                                      gTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCg               >  2:976280/1‑150 (MQ=255)
                                                                                                                                      gTTGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGGTGCCCGACCGACCAGATTCGCTTCCg               >  2:2073293/1‑150 (MQ=255)
                                                                                                                                       ttGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCgg              >  2:2172645/1‑150 (MQ=255)
                                                                                                                                       ttGTCGCCGTTATGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCgg              >  2:97124/1‑150 (MQ=255)
                                                                                                                                       ttGTCGCCATACCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCgg              <  1:2634228/150‑1 (MQ=255)
                                                                                                                                       ttGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCgg              <  1:2129587/150‑1 (MQ=255)
                                                                                                                                        tGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGc             >  2:2587073/1‑150 (MQ=255)
                                                                                                                                            gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaa         >  2:2774079/1‑150 (MQ=255)
                                                                                                                                            gCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaa         >  2:257655/1‑150 (MQ=255)
                                                                                                                                             ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa        <  1:2862664/150‑1 (MQ=255)
                                                                                                                                             ccATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACaaa        >  1:900881/1‑150 (MQ=255)
                                                                                                                                              cATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAg       <  2:2448856/150‑1 (MQ=255)
                                                                                                                                                 aaCGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGccc    >  2:2946463/1‑150 (MQ=255)
                                                                                                                                                   cGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCa  <  2:2839430/150‑1 (MQ=255)
                                                                                                                                                   cGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCa  >  2:2533296/1‑150 (MQ=255)
                                                                                                                                                   cGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCa  <  1:1131502/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
TATATGGGGACCAACGCTGTTTCGTGTGCCGTGGCAACCTGGAGGTGCCAACCTTAAACTTCGGGGGAATATTCTATACACAACGACGGGGGATGTCGTTAGCCACGGGAGATTTATCTCATAAATAATTCACGTTGTCGCCATAACGGCGACAACGTGAACGAAGATGGCTATTAATGGATGCCGCCAATGCGACTTAAGCGCAGACCAGTCGGCCATTCGCCTTCTTGCTTATCGAAGCTCATCCAGATAGCCGTTGCCCGACCGACCAGATTCGCTTCCGGCACAAAGCCCCA  >  NC_000913/2704162‑2704457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: