Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,768,615 G→T 6.0% intergenic (+42/‑50) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,768,6150GT 6.0% 730.0 / 25.1 199intergenic (+42/‑50)yfjP/yfjQCP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (90/97);  new base T (7/5);  total (97/102)
Fisher's exact test for biased strand distribution p-value = 5.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 200 total aligned reads displayed.

CGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCA  >  NC_000913/2768467‑2768764
                                                                                                                                                    |                                                                                                                                                     
cGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTACTGAGTGTTTAATTCTGCCCGATTATTCTCCGAGCCCTGTCGCAAGt                                                                                                                                                      >  1:1661276/1‑150 (MQ=255)
  gTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGc                                                                                                                                                    >  2:80403/1‑150 (MQ=255)
  gTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCATGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGc                                                                                                                                                    >  2:640742/1‑150 (MQ=255)
   tAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCg                                                                                                                                                   <  1:1134694/150‑1 (MQ=255)
      gCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCa                                                                                                                                                >  2:1074365/1‑150 (MQ=255)
       cACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                               <  1:1184759/150‑1 (MQ=255)
       cACCTTTCCCCTTAGTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                               <  1:2700501/150‑1 (MQ=255)
        aCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAgg                                                                                                                                              <  1:2823183/150‑1 (MQ=255)
        aCCTTTCCCCTGATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAgg                                                                                                                                              <  2:2794289/150‑1 (MQ=21)
         ccTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCAAGCCCTGCCGCACTTGCGACAggg                                                                                                                                             <  1:255297/150‑1 (MQ=25)
               cccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttct                                                                                                                                       >  1:1521882/1‑150 (MQ=255)
                 cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttcttt                                                                                                                                     >  2:2742236/1‑150 (MQ=255)
                 cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttcttt                                                                                                                                     >  1:2207472/1‑150 (MQ=255)
                 cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAAGTCTGCCCTATGATTCTCCGAGCCCTGTCGCACGGGCGGCAGGGctttctgt                                                                                                                                     >  1:57424/1‑148 (MQ=255)
                  ttATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTa                                                                                                                                    >  1:1742261/1‑150 (MQ=255)
                  ttATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTa                                                                                                                                    >  2:1716418/1‑150 (MQ=255)
                       cccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGt                                                                                                                               >  1:1562369/1‑150 (MQ=255)
                         cGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGttt                                                                                                                             >  1:549572/1‑150 (MQ=255)
                         cGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGttt                                                                                                                             <  1:867208/150‑1 (MQ=255)
                           ccccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTa                                                                                                                           <  1:2669974/150‑1 (MQ=255)
                            cccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAc                                                                                                                          <  1:2297631/150‑1 (MQ=255)
                             ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                         <  1:3130968/150‑1 (MQ=255)
                             ccGGTGCGGACCATTAGTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTAGTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                         <  1:720488/150‑1 (MQ=255)
                              cGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAccc                                                                                                                        >  1:2448996/1‑150 (MQ=255)
                                 tGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATa                                                                                                                     <  2:2407608/150‑1 (MQ=255)
                                     gACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTga                                                                                                                 >  1:1392461/1‑150 (MQ=255)
                                     gACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTga                                                                                                                 >  2:1052646/1‑150 (MQ=255)
                                     gACCATAATTCAGGCCGTGCGTTCCACGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTAGTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTga                                                                                                                 >  2:1442060/1‑150 (MQ=255)
                                        cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgagga                                                                                                              >  1:2644434/1‑150 (MQ=255)
                                         attattCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTATTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgaggag                                                                                                             <  1:808205/150‑1 (MQ=255)
                                                aGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCtt                                                                                                      >  2:274756/1‑150 (MQ=255)
                                                  gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTAt                                                                                                    <  2:2026422/150‑1 (MQ=255)
                                                  gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTAt                                                                                                    <  1:2762546/150‑1 (MQ=255)
                                                  gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTAt                                                                                                    <  2:416363/150‑1 (MQ=255)
                                                     gTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                 >  1:2893018/1‑150 (MQ=255)
                                                     gTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                 <  1:1485380/150‑1 (MQ=255)
                                                         gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                             <  2:1855949/150‑1 (MQ=255)
                                                         gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTGTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                             >  1:100923/1‑150 (MQ=255)
                                                         gTTCCTCGGTGGTGTCAGTGGCCAGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGACGCTAGTGCGGCAGGGCGTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                             >  2:1196884/1‑150 (MQ=255)
                                                           tCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCt                                                                                           >  1:82682/1‑150 (MQ=255)
                                                             cTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTgg                                                                                         >  1:1610625/1‑150 (MQ=255)
                                                              tCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGc                                                                                        >  1:1146483/1‑150 (MQ=255)
                                                                 gtggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTc                                                                                     >  1:2660984/1‑150 (MQ=255)
                                                                   ggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTcgc                                                                                   <  2:2284615/150‑1 (MQ=255)
                                                                        cAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCttt                                                                              <  1:197308/150‑1 (MQ=255)
                                                                            ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                          <  1:3188078/150‑1 (MQ=255)
                                                                            ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                          <  1:2345467/150‑1 (MQ=255)
                                                                            ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                          <  1:1147718/150‑1 (MQ=255)
                                                                             gCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcgc                                                                         <  2:1287565/150‑1 (MQ=255)
                                                                             gCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcgc                                                                         >  1:363494/1‑150 (MQ=255)
                                                                                 gcgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCa                                                                     >  2:1283765/1‑150 (MQ=255)
                                                                                  cgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCaa                                                                    >  1:3220918/1‑150 (MQ=255)
                                                                                     tGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATc                                                                 <  1:1254866/150‑1 (MQ=255)
                                                                                     tGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATc                                                                 <  1:1823942/150‑1 (MQ=255)
                                                                                          gggATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAtt                                                            <  2:2498557/150‑1 (MQ=255)
                                                                                            gATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCg                                                          <  1:2793914/150‑1 (MQ=255)
                                                                                              tttCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGcc                                                        <  1:1269706/150‑1 (MQ=255)
                                                                                              tttCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGcc                                                        <  2:549572/150‑1 (MQ=255)
                                                                                               ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                       >  2:2874463/1‑150 (MQ=255)
                                                                                               ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                       >  2:2570657/1‑150 (MQ=255)
                                                                                                  ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                    >  2:1558199/1‑150 (MQ=255)
                                                                                                  ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                    >  2:2081164/1‑150 (MQ=255)
                                                                                                  ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                    >  2:1409230/1‑150 (MQ=255)
                                                                                                     ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccg                                                 >  1:575362/1‑150 (MQ=255)
                                                                                                     ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccg                                                 >  2:1653333/1‑150 (MQ=255)
                                                                                                     ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTAGGCGCTGCAAATCTTATTCGCCGTGAcat                                                 >  1:2000429/1‑148 (MQ=255)
                                                                                                        tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                              <  2:1945714/150‑1 (MQ=255)
                                                                                                        tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                              >  1:2180/1‑150 (MQ=255)
                                                                                                              tttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAc                                        <  1:3222189/150‑1 (MQ=255)
                                                                                                               ttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAcc                                       >  2:1508706/1‑150 (MQ=255)
                                                                                                                tAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAccc                                      >  2:309514/1‑150 (MQ=255)
                                                                                                                  aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                    <  2:286469/150‑1 (MQ=255)
                                                                                                                  aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                    <  1:2082075/150‑1 (MQ=255)
                                                                                                                  aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                    <  1:1922755/150‑1 (MQ=255)
                                                                                                                  aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCGTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                    <  1:2494377/150‑1 (MQ=255)
                                                                                                                      tGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAag                                <  1:240817/150‑1 (MQ=255)
                                                                                                                      tGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAag                                >  2:57960/1‑150 (MQ=255)
                                                                                                                       gCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAaga                               <  1:1405042/150‑1 (MQ=255)
                                                                                                                       gCCCTATTAGTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAagn                               <  1:3041484/150‑2 (MQ=255)
                                                                                                                         ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                             >  2:1758069/1‑150 (MQ=255)
                                                                                                                         ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAATTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                             >  1:2496071/1‑150 (MQ=255)
                                                                                                                              tattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAttt                        >  2:432639/1‑150 (MQ=255)
                                                                                                                              tattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAttt                        >  1:3204958/1‑150 (MQ=255)
                                                                                                                                 tctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcgc                     >  2:845161/1‑150 (MQ=255)
                                                                                                                                 tctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcgc                     <  1:1168665/150‑1 (MQ=255)
                                                                                                                                   tcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt                   >  2:343986/1‑150 (MQ=255)
                                                                                                                                   agaaaGCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt                   >  2:2215964/5‑150 (MQ=21)
                                                                                                                                      gAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt                <  2:292469/150‑1 (MQ=255)
                                                                                                                                      gAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAATTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt                <  2:737048/150‑1 (MQ=255)
                                                                                                                                       aGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggtg               <  2:3031971/150‑1 (MQ=255)
                                                                                                                                        gCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGc              <  2:1795882/150‑1 (MQ=255)
                                                                                                                                          ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCACATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc            >  2:1260092/1‑150 (MQ=255)
                                                                                                                                          ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc            >  2:1590589/1‑150 (MQ=255)
                                                                                                                                          ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACTCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc            >  2:2629276/1‑150 (MQ=21)
                                                                                                                                          ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc            >  2:3097703/1‑150 (MQ=255)
                                                                                                                                          ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc            >  2:3035212/1‑150 (MQ=255)
                                                                                                                                            tGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGATGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAg          <  1:1910748/150‑1 (MQ=255)
                                                                                                                                            tGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAg          <  1:2798982/150‑1 (MQ=255)
                                                                                                                                             gTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGc         <  2:717705/150‑1 (MQ=255)
                                                                                                                                                  aaGTGCGTCAGTGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTAtt    <  2:31872/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
CGGTAAGCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCA  >  NC_000913/2768467‑2768764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: