Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,768,620 G→A 6.1% intergenic (+47/‑45) yfjP → / → yfjQ CP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,768,6200GA 6.1% 675.0 / 21.5 196intergenic (+47/‑45)yfjP/yfjQCP4‑57 prophage; 50S ribosome‑binding GTPase family protein/CP4‑57 prophage; uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base G (86/97);  new base A (7/5);  total (93/103)
Fisher's exact test for biased strand distribution p-value = 5.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 209 total aligned reads displayed.

GCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAG  >  NC_000913/2768473‑2768769
                                                                                                                                                   |                                                                                                                                                     
 cACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                                    <  1:1184641/150‑1 (MQ=255)
 cACCTTTCCCCTTAGTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAg                                                                                                                                                    <  1:2700501/150‑1 (MQ=255)
  aCCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAgg                                                                                                                                                   <  1:2823152/150‑1 (MQ=255)
  aCCTTTCCCCTGATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGCCGCACTTGCGACAgg                                                                                                                                                   <  2:2794289/150‑1 (MQ=21)
   ccTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCAAGCCCTGCCGCACTTGCGACAggg                                                                                                                                                  <  1:255297/150‑1 (MQ=25)
         cccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttct                                                                                                                                            >  2:3032148/1‑150 (MQ=255)
          ccTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttctt                                                                                                                                           >  1:7652/1‑150 (MQ=255)
           cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttcttt                                                                                                                                          >  2:2742236/1‑150 (MQ=255)
           cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttcttt                                                                                                                                          >  1:1394809/1‑150 (MQ=255)
           cTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGctttcttt                                                                                                                                          >  1:2207472/1‑150 (MQ=255)
            ttATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTa                                                                                                                                         >  1:1742261/1‑150 (MQ=255)
                 cccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGt                                                                                                                                    >  1:1562137/1‑150 (MQ=255)
                  ccGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTGTCTTTATTTGtt                                                                                                                                   >  1:3011191/1‑150 (MQ=255)
                     ccccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTa                                                                                                                                <  1:2669974/150‑1 (MQ=255)
                     ccccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTa                                                                                                                                <  1:1920988/150‑1 (MQ=255)
                      cccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAc                                                                                                                               <  2:1063724/150‑1 (MQ=255)
                       ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                              <  1:566785/150‑1 (MQ=255)
                       ccGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAcc                                                                                                                              <  1:3130968/150‑1 (MQ=255)
                        cGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTAccc                                                                                                                             >  1:2448996/1‑150 (MQ=255)
                           tGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATa                                                                                                                          <  2:2407608/150‑1 (MQ=255)
                            gCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATAc                                                                                                                         <  2:552508/150‑1 (MQ=255)
                                aCCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgag                                                                                                                     >  2:387687/1‑150 (MQ=255)
                                  cATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTgagga                                                                                                                   >  1:2644434/1‑150 (MQ=255)
                                          aGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCtt                                                                                                           >  2:625356/1‑150 (MQ=255)
                                          aGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCtt                                                                                                           >  2:274756/1‑150 (MQ=255)
                                            gCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTAt                                                                                                         <  2:2026422/150‑1 (MQ=255)
                                              cGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGa                                                                                                       <  2:1436620/150‑1 (MQ=255)
                                              cGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGa                                                                                                       <  2:1652166/150‑1 (MQ=255)
                                               gTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                      <  2:2644434/150‑1 (MQ=255)
                                               gTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGAc                                                                                                      <  1:1485319/150‑1 (MQ=255)
                                                   gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                                  <  2:1855949/150‑1 (MQ=255)
                                                   gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTGTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGt                                                                                                  >  1:100923/1‑150 (MQ=255)
                                                   gTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGGGCGGCAGGGCTTTCTTTATTTGTGTTACCCATACTGAGGAGTCTGCTTATGACCCgg                                                                                                  >  2:789716/1‑149 (MQ=255)
                                                       cTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTgg                                                                                              >  1:1610625/1‑150 (MQ=255)
                                                       cTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTgg                                                                                              <  2:3026685/150‑1 (MQ=255)
                                                        tCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCGTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGc                                                                                             >  2:2591375/1‑150 (MQ=255)
                                                           gtggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTc                                                                                          >  1:2660984/1‑150 (MQ=255)
                                                             ggtgTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTcgc                                                                                        <  2:2284600/150‑1 (MQ=255)
                                                                gtCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCt                                                                                     >  1:2318237/1‑150 (MQ=255)
                                                                 gcAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCtt                                                                                    <  1:1156751/149‑1 (MQ=255)
                                                                  cAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCttt                                                                                   <  1:197308/150‑1 (MQ=255)
                                                                      ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                               <  1:3188078/150‑1 (MQ=255)
                                                                      ggCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcg                                                                               <  1:1147711/150‑1 (MQ=255)
                                                                       gCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcgc                                                                              <  2:1287565/150‑1 (MQ=255)
                                                                       gCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGgcgc                                                                              <  2:1287373/150‑1 (MQ=255)
                                                                         ccGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTg                                                                            >  1:1263625/1‑150 (MQ=255)
                                                                            cgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCaa                                                                         >  1:3220918/1‑150 (MQ=255)
                                                                            cgcTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCaa                                                                         >  1:2377438/1‑150 (MQ=255)
                                                                               tGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATc                                                                      <  1:1823942/150‑1 (MQ=255)
                                                                                    gggATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAtt                                                                 <  2:2498557/150‑1 (MQ=255)
                                                                                    gggATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACCGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTAtt                                                                 >  1:1487603/1‑150 (MQ=255)
                                                                                      gATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCg                                                               <  1:2793796/150‑1 (MQ=255)
                                                                                      gATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCg                                                               <  1:2793972/150‑1 (MQ=255)
                                                                                      gATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCg                                                               <  1:824903/150‑1 (MQ=255)
                                                                                        tttCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATTCTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGcc                                                             <  1:187944/150‑1 (MQ=255)
                                                                                         ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                            >  2:2874463/1‑150 (MQ=255)
                                                                                         ttcttcttcTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCg                                                            >  2:1354534/1‑150 (MQ=255)
                                                                                            ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                         >  2:2081164/1‑150 (MQ=255)
                                                                                            ttcttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGa                                                         <  2:1084861/150‑1 (MQ=255)
                                                                                               ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccg                                                      >  1:575337/1‑150 (MQ=255)
                                                                                               ttctGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTAGGCGCTGCAAATCTTATTCGCCGTGAcat                                                      >  1:2000429/1‑148 (MQ=255)
                                                                                                  tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                                   >  1:2180/1‑150 (MQ=255)
                                                                                                  tGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGAccgtcc                                                   <  2:1945714/150‑1 (MQ=255)
                                                                                                      tgtTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTa                                               >  1:124036/1‑150 (MQ=255)
                                                                                                        tttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAc                                             <  1:3222189/150‑1 (MQ=255)
                                                                                                         ttAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCTCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAcc                                            >  1:2853700/1‑150 (MQ=255)
                                                                                                          tAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAAccc                                           >  2:309642/1‑150 (MQ=255)
                                                                                                            aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                         <  1:2082075/150‑1 (MQ=255)
                                                                                                            aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                         <  2:286469/150‑1 (MQ=255)
                                                                                                            aTTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCGTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGt                                         <  1:2494377/150‑1 (MQ=255)
                                                                                                              tCTGCCCTATTATTCTCCGAGCACTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGa                                       <  1:459242/150‑1 (MQ=255)
                                                                                                                tGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAag                                     <  1:240817/150‑1 (MQ=255)
                                                                                                                 gCCCTATTAGTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAagn                                    <  1:3041484/150‑2 (MQ=255)
                                                                                                                   ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGTAGAGt                                  <  1:1925622/150‑1 (MQ=255)
                                                                                                                   ccTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGt                                  >  2:1758069/1‑150 (MQ=255)
                                                                                                                        tattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAttt                             >  1:1387804/1‑150 (MQ=255)
                                                                                                                        tattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAttt                             >  2:541203/1‑150 (MQ=255)
                                                                                                                        tattCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTAttt                             >  1:3204958/1‑150 (MQ=255)
                                                                                                                         attCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTc                            >  2:3063059/1‑150 (MQ=255)
                                                                                                                          ttCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcg                           <  1:1142236/150‑1 (MQ=255)
                                                                                                                          ttCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcg                           >  1:2515488/1‑150 (MQ=255)
                                                                                                                           tctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcgc                          >  2:845161/1‑150 (MQ=255)
                                                                                                                           tctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcgc                          <  1:834684/150‑1 (MQ=255)
                                                                                                                            ctcCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTcgcg                         <  2:1481181/150‑1 (MQ=255)
                                                                                                                             agaaaGCCCTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgt                        >  2:2215964/5‑150 (MQ=21)
                                                                                                                              ccGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtg                       <  1:644977/150‑1 (MQ=255)
                                                                                                                                gAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTAATTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCgtggt                     <  2:737048/150‑1 (MQ=255)
                                                                                                                                  gCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGc                   <  2:1795882/150‑1 (MQ=255)
                                                                                                                                   cccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGcc                  >  1:932989/1‑150 (MQ=255)
                                                                                                                                    ccTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTAGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc                 >  2:1589996/1‑150 (MQ=255)
                                                                                                                                    ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc                 >  2:1270041/1‑150 (MQ=255)
                                                                                                                                    ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc                 >  2:42451/1‑150 (MQ=255)
                                                                                                                                    ccTGCCGCACTTGCGACAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCAGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGccc                 >  2:168720/1‑150 (MQ=21)
                                                                                                                                       gTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGc              <  2:717705/150‑1 (MQ=255)
                                                                                                                                            aaGTGCGTCAGTGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTAtt         <  2:31872/150‑1 (MQ=255)
                                                                                                                                                gCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGt     <  2:1543839/150‑1 (MQ=255)
                                                                                                                                                gCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGt     <  2:1521882/150‑1 (MQ=255)
                                                                                                                                                  ggCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGa   >  2:3237742/1‑150 (MQ=255)
                                                                                                                                                  ggCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGa   <  2:233159/150‑1 (MQ=255)
                                                                                                                                                   gCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAg  <  1:510100/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                     
GCACCTTTCCCCTTATTCCCGCCCCGGTGCGGACCATTATTCAGGCCGTGCGTTCCTCGGTGGTGTCAGTGGCCCGCGCTGTCTGGGATTTCTTCTTCTGAGTGTTTAATTCTGCCCTATTATTCTCCGAGCCCTGTCGCAAGTGCGGCAGGGCTTTCTTTATTTGTTTTACCCATACTGAGGAGTCTGCTTATGACCCGTCTGGCTTCGCGCTTTGGCGCTGCAAATCTTATTCGCCGTGACCGTCCGTTAACCCGTGAAGAGTTATTTCGCGTGGTGCCCAGCGTATTCAGTGAG  >  NC_000913/2768473‑2768769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: