Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,233,660 A→C 6.1% T666P (ACA→CCA)  fadH → 2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,233,6600AC 6.1% 393.3 / 2.7 130T666P (ACA→CCA) fadH2,4‑dienoyl‑CoA reductase, NADH and FMN‑linked
Reads supporting (aligned to +/- strand):  ref base A (73/47);  new base C (3/7);  total (76/54)
Fisher's exact test for biased strand distribution p-value = 9.19e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 133 total aligned reads displayed.

TAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCG  >  NC_000913/3233511‑3233809
                                                                                                                                                     |                                                                                                                                                     
tAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGaa                                                                                                                                                       >  1:2989646/1‑150 (MQ=255)
     gTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACgg                                                                                                                                                  >  1:284050/1‑150 (MQ=255)
     gTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACgg                                                                                                                                                  <  1:3126435/150‑1 (MQ=255)
     gTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACgg                                                                                                                                                  >  1:837522/1‑150 (MQ=255)
      tGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCACTTGCCCAGGGAACACGGc                                                                                                                                                 >  1:2670769/1‑150 (MQ=255)
       ggACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCt                                                                                                                                                <  1:1877848/150‑1 (MQ=255)
        gACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTg                                                                                                                                               <  2:1201512/150‑1 (MQ=255)
          cAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGgc                                                                                                                                             <  2:338074/150‑1 (MQ=255)
           aaTGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGgcg                                                                                                                                            >  1:2580111/1‑150 (MQ=255)
           aaTGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGgcg                                                                                                                                            >  1:2050429/1‑150 (MQ=255)
                 gtgATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGgag                                                                                                                                      >  2:2980922/1‑150 (MQ=255)
                 gtgATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGgag                                                                                                                                      >  2:2980892/1‑150 (MQ=255)
                       tGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTaa                                                                                                                                >  2:2865590/1‑150 (MQ=255)
                       tGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTaa                                                                                                                                >  2:1308483/1‑150 (MQ=255)
                       tGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCGATGGAGCTGGACGCACGACGGGCACTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTaa                                                                                                                                >  2:1824845/1‑150 (MQ=255)
                        gcgcAGGGCAAGAGCCAAACCGCGCGCGGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTaaa                                                                                                                               <  2:1488535/150‑1 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAAt                                                                                                                              >  2:2837608/1‑150 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAAt                                                                                                                              >  2:3013652/1‑150 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAAt                                                                                                                              >  2:1219700/1‑150 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAAt                                                                                                                              >  2:1219685/1‑150 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAAt                                                                                                                              >  1:1057708/1‑150 (MQ=255)
                         cgcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCGGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCACTTGCCCAGGGACCACGGCTGGCGCTGGAGATTTcaag                                                                                                                              >  2:2438440/1‑149 (MQ=255)
                          gcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATc                                                                                                                             <  2:438750/150‑1 (MQ=255)
                          gcAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATc                                                                                                                             <  2:954689/150‑1 (MQ=255)
                           cAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCg                                                                                                                            >  2:390951/1‑150 (MQ=255)
                             gggCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGtt                                                                                                                          <  1:1036586/150‑1 (MQ=255)
                                  agagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTg                                                                                                                     >  1:3219414/1‑150 (MQ=255)
                                  agagCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTg                                                                                                                     >  1:3219412/1‑150 (MQ=255)
                                    agCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGaa                                                                                                                   <  1:2839963/150‑1 (MQ=255)
                                    agCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGaa                                                                                                                   <  2:174356/150‑1 (MQ=255)
                                            cgcgcgCTGGCGCAACCGCTGATTGATAGCGGTAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGa                                                                                                           <  1:3120431/150‑1 (MQ=255)
                                            cgcgcgCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGa                                                                                                           <  1:1738135/150‑1 (MQ=255)
                                                 gcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGACCACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                      >  1:277390/1‑150 (MQ=255)
                                                 gcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                      <  2:3127072/150‑1 (MQ=255)
                                                 gcTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCaa                                                                                                      >  1:3129452/1‑150 (MQ=255)
                                                  cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                     <  2:633491/150‑1 (MQ=255)
                                                  cTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAAc                                                                                                     <  2:1403517/150‑1 (MQ=255)
                                                        cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                               <  2:2565371/150‑1 (MQ=255)
                                                        cAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATcc                                                                                               <  2:2565334/150‑1 (MQ=255)
                                                          aCCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCGTTGCATCCgg                                                                                             <  2:225423/150‑1 (MQ=255)
                                                               tgattgatAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGt                                                                                        >  2:2272371/1‑150 (MQ=255)
                                                                    gatAGCGGGAAAACGGTGAATTTATTGGGCGGCTGCGATGTGGGTATGGAGCTGGACGCACGACGGGCAATGGCCCTGGGAACACGGCTGGCGGTGGATTTTTAAATCGTTATTTGACGCGCCGGAGGAAACGAATCCGGCACGTCGCGt                                                                                   <  2:522980/150‑1 (MQ=255)
                                                                      tAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTa                                                                                 >  1:512492/1‑150 (MQ=255)
                                                                      tAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTa                                                                                 >  1:1193342/1‑150 (MQ=255)
                                                                         cGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATc                                                                              >  1:652638/1‑150 (MQ=255)
                                                                         cGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATc                                                                              >  1:174852/1‑150 (MQ=255)
                                                                          gggAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCa                                                                             >  2:3170470/1‑150 (MQ=255)
                                                                            taaaaGGGTGCTTTTATTTGGCGGCTGCTTTGTGGCTTTGGTGCTGGACGCACGCCGGGAATTTGCCCAGGTAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                           <  2:1613079/149‑1 (MQ=255)
                                                                            gAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAAt                                                                           >  2:490747/1‑150 (MQ=255)
                                                                                  ggTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACaa                                                                     >  2:1628213/1‑150 (MQ=255)
                                                                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                    >  2:727173/1‑150 (MQ=255)
                                                                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                    >  2:727045/1‑150 (MQ=255)
                                                                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                    >  2:195613/1‑150 (MQ=255)
                                                                                   gTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAg                                                                    >  2:726897/1‑150 (MQ=255)
                                                                                    tGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAgg                                                                   >  1:2576401/1‑150 (MQ=255)
                                                                                    tGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAgg                                                                   >  1:2576473/1‑150 (MQ=255)
                                                                                      cATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCg                                                                 >  1:2590628/1‑150 (MQ=255)
                                                                                              tGGCGGCTGCGATGTGGCTATGGAGGTGGACTCACGACGGGCATTTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAt                                                         <  2:2956941/150‑1 (MQ=255)
                                                                                                ggggcTGCTTTGGGGCTATGGAGGTGGACGCACGACGGGCATTTGCCCAGGGAACCGGGCGGGCGCTGTTTTTTTAAATCGTTATTTGACGCGCGGTATGCAACGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGc                                                       <  2:2580111/148‑1 (MQ=255)
                                                                                                gcggcTGCTTTGGGGCTTTGGAGGTGGACGCACGACGGGCAATTGCCCAGGTAACACGGCTGGCGCTGTATATTTAATTCTTTATTTGAACCGCCGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGc                                                       <  2:2050429/150‑1 (MQ=255)
                                                                                                     tGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAAt                                                  >  1:2107259/1‑150 (MQ=255)
                                                                                                        tttgtgGGTTTGGTGGTGGCCGCACGCCGGGAATTTGCCCTGTCAACAGGGGGGGCGCTGTATATTTAAATCGTTATTTGACGCGCGGTATCAAACGCATCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATcgc                                               <  1:2132332/148‑1 (MQ=255)
                                                                                                        gATGGGGCTTTGGCGGTGGACGCACGCGGGGAATTTGCCCTGGTAACAGGGGTGGGGCTGTATTTTTAAATGGTTATTTGACGCGCGGTATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATcgc                                               <  2:97746/150‑1 (MQ=255)
                                                                                                             ggCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGc                                          >  1:1440042/1‑150 (MQ=255)
                                                                                                              gCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGcc                                         >  1:2324101/1‑150 (MQ=255)
                                                                                                                 aTGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAt                                      >  1:881066/1‑150 (MQ=255)
                                                                                                                  gggAGCTGGACGCCCGCCGGGAAATGGCCCTGGCACCACGGGTGGCGCTGGATTTTTAAACCGTTATTTCAAGCGCGGGATGCCCCGCACCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtt                                     <  1:757676/149‑1 (MQ=255)
                                                                                                                      gCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGATTTTTAAATCGTTATTGGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAAtttttt                                 <  2:2679509/150‑1 (MQ=255)
                                                                                                                       caggACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGAATAAAATCGTTATTTGAAGCGCCGGAAGCAAAGAATCCGGCACGTCGAGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                >  1:620549/3‑150 (MQ=255)
                                                                                                                       cTGGACGCACGAGGGGAATTTGCCCAGGTAACCGGGCTGGCGCTGTATATTTAAATCTTTTTTGGACGCGCGGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTa                                <  1:2579720/150‑1 (MQ=255)
                                                                                                                        tGGACGCACGCGGGGAATTTGCCCAGGCAACCCGGGTGGCGCTGTAGATTTAATTCGTTATTTGACGGGCGGTTCGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAg                               <  2:688528/150‑1 (MQ=255)
                                                                                                                         ggccGCACGCCGGGCATTGGCCCTGGTACCCGGGGTGGGGGTGGATATTTAATTGGTTTTTGGCCGCGCCGGATGCACCGCTGCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                              <  1:2312841/147‑1 (MQ=255)
                                                                                                                         ggACGCACGAGGGGCATTTGCCCTGGGAACCGGGCTGGCGGTGTTGATTTAAATGTTTTTTTGACGCGCGGGATGAAACCCACCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGc                              <  1:1319230/150‑1 (MQ=255)
                                                                                                                             gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                          >  2:2470637/1‑150 (MQ=255)
                                                                                                                             gCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCgtgt                          >  2:780826/1‑150 (MQ=255)
                                                                                                                               acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                        >  2:341361/1‑150 (MQ=255)
                                                                                                                               acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                        >  1:935802/1‑150 (MQ=255)
                                                                                                                               acgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAAAGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTc                        >  2:37516/1‑150 (MQ=255)
                                                                                                                                cgacgGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTcc                       >  1:2533345/1‑150 (MQ=255)
                                                                                                                                 gacgGGCATTTGCCCTGGGAACACGGCTGGCGCGGTAGATTTAAATCGTTATTTGAAGCGCGGGATGCACCGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCa                      <  2:2583352/150‑1 (MQ=255)
                                                                                                                                   ggggCAAGTGCCCTGGGACCCCGGCTGGCGCGGGAGTTTTAATTGGTTATTGGCCGCGCCGGATGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGc                    <  1:1876516/149‑1 (MQ=255)
                                                                                                                                   cgGGCACTGGCCCCGGGACCCCGGCGGGCGCGGGAGATTTAACTCGTTTTTTGACGCGCGGGCTGCACCGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGc                    <  1:1876415/150‑1 (MQ=255)
                                                                                                                                    gggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCg                   >  1:696068/1‑150 (MQ=255)
                                                                                                                                    gggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCg                   >  1:1486051/1‑150 (MQ=255)
                                                                                                                                     ggCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGTAGCGCCGGATGCGACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGt                  >  2:1893687/1‑150 (MQ=255)
                                                                                                                                      gaatttGCCCAGGTAACACGGCTGGCGCTGTTTATTTAAATCGTTTTTTGACGCGCCGTATGCAACGCATCCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                 <  1:306593/146‑1 (MQ=255)
                                                                                                                                      gCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                 >  1:2211593/1‑150 (MQ=255)
                                                                                                                                      caaaTTCCCCAGGCAACACGGCTGGCGCTGTATATTAAAATCTTTATTTCAAGCGCCGTAACAACCCCAACCGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGtt                 <  1:1571192/148‑1 (MQ=255)
                                                                                                                                            gCCCTGGGAACCGGGGTGGCGCTGTTGATTTATATCGTTATTTGACGCGCCGGTTGCAACGCCCCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc           <  1:490747/150‑1 (MQ=255)
                                                                                                                                            gCCCTGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc           <  2:265346/150‑1 (MQ=255)
                                                                                                                                            gCCCCGGGAACCCGGCTGGCGCTGGAGATTTAAATCGTTCTTTGCCGCGCCGGAGGCACCGCAGCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc           <  2:172816/150‑1 (MQ=255)
                                                                                                                                            gCCCAGGGACCACGGCTGGCGCTGGAGATTTAAATGGTTATTTGAAGCGCCGGATGCAACGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc           <  2:2670769/150‑1 (MQ=255)
                                                                                                                                            gCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCTTCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTc           <  2:2421343/150‑1 (MQ=255)
                                                                                                                                             cccTGGCAACAGGGCTGGGGGTGGTGTTTTAATTGGTTATTTGATGCGCCGGATCCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTcc          <  2:2501437/150‑1 (MQ=255)
                                                                                                                                              cctgggAACAGGGCTGGCGCTGTAGATTTAATTGTTTTTTTGACGCGCCTGATGCACCGAATCGGCCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct         <  2:2852159/147‑1 (MQ=255)
                                                                                                                                              ccAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCct         <  2:2173154/150‑1 (MQ=255)
                                                                                                                                                   gAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTTAATCGCCGGATGCAAAGCATCCGGCACGTACCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCAAGCGTTAATTTCCTCTTcc    >  1:1697279/1‑150 (MQ=255)
                                                                                                                                                   gAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTcc    >  1:2776602/1‑150 (MQ=255)
                                                                                                                                                     acacGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCg  >  2:2087243/1‑150 (MQ=255)
                                                                                                                                                     acacGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCg  >  2:2593600/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TAGCAGTGGACAATGTGGTGATCTGCGCAGGGCAAGAGCCAAACCGCGCGCTGGCGCAACCGCTGATTGATAGCGGGAAAACGGTGCATTTAATTGGCGGCTGCGATGTGGCTATGGAGCTGGACGCACGACGGGCAATTGCCCAGGGAACACGGCTGGCGCTGGAGATTTAAATCGTTATTTGAAGCGCCGGATGCAACGCATCCGGCACGTCGCGTTAATCAATAAACAAGGCGGGAGAAATGCCGAATCGCGTTGCCAATTTTTTAGCGTGTTCCAGCGTTAATTTCCTCTTCCCG  >  NC_000913/3233511‑3233809

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: