Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,005 A→C 10.9% intergenic (‑124/+64) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0050AC10.9% 566.8 / 17.1 210intergenic (‑124/+64)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base A (154/25);  new base C (2/25);  total (159/51)
Fisher's exact test for biased strand distribution p-value = 3.36e-16
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 228 total aligned reads displayed.

TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153
                                                                                                                                                     |                                                                                                                                                    
ttcttcTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACaaa                                                                                                                                                      >  1:2072510/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                   >  1:1037913/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAagag                                                                                                                                                   >  1:3130537/1‑150 (MQ=255)
   ttctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAACATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAACACAAagag                                                                                                                                                   >  2:4160542/1‑150 (MQ=255)
    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAgg                                                                                                                                                  >  2:3486083/1‑150 (MQ=255)
    tctcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAgg                                                                                                                                                  <  2:1163967/150‑1 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                >  1:1885921/1‑150 (MQ=255)
      tcttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGaa                                                                                                                                                >  1:1409152/1‑150 (MQ=255)
        ttcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTAGTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGACAACAAAGAGGACAg                                                                                                                                              >  2:1666211/1‑150 (MQ=255)
         tcCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                                                             >  1:4669314/1‑150 (MQ=255)
          ccGGAGTACTGTGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                                                                            <  2:3553145/150‑1 (MQ=255)
           cGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAACACAAAGAGGAAAgggg                                                                                                                                           >  1:4397782/1‑150 (MQ=255)
            ggAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg                                                                                                                                          <  2:108937/150‑1 (MQ=255)
              aGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                                                                                                                        <  2:5353843/150‑1 (MQ=255)
                tACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggg                                                                                                                                      >  1:3902758/1‑150 (MQ=255)
                   tGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCt                                                                                                                                   >  1:4312555/1‑150 (MQ=255)
                     ggCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAAt                                                                                                                                >  1:2877027/1‑150 (MQ=255)
                          ttCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGc                                                                                                                            >  1:4136714/1‑150 (MQ=255)
                           tCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCGAATCGGCAg                                                                                                                          >  1:3924697/1‑150 (MQ=255)
                           tCGCACCTCACCCTCTGCATGCAATATACTAGAGGGGGGTATTCTATCGGTCAATGCATACCCCCCGATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGGGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCa                                                                                                                           <  2:1896779/150‑1 (MQ=255)
                            cGCACCTCATCCTCTGCATGCAATAACCTCTCGGGGGGTATACTCTAGGACAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTAGCCGGTGAATGGGGTCGGAAAACACCGCGGAAAGGGGGGGGGGCTAATCGGCAg                                                                                                                          <  2:5381625/150‑1 (MQ=255)
                            cGCACCTCATCATCTGCATGCAATATACTATTGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATACCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAg                                                                                                                          <  2:3153589/150‑1 (MQ=255)
                            cGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAg                                                                                                                          >  1:2698901/1‑150 (MQ=255)
                              cACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGAATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCCGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggg                                                                                                                        >  1:2146792/1‑150 (MQ=255)
                               aCCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGa                                                                                                                       >  2:4074460/1‑150 (MQ=255)
                                 cTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                                                                     >  1:817137/1‑150 (MQ=255)
                                   catcatCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                                                   >  2:1514328/1‑150 (MQ=255)
                                   caacatcTTCATGCAATATACATTCGGGGTTTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACCCTGGAGAATACCATTTAGCCGGTTAATGTGGTCGGAAAACCCCGCGCAAAGGGGGGGGGGCTAATCGGCAGGGAAGgc                                                                                                                   <  2:4971885/147‑1 (MQ=255)
                                       atcTGCATGCACTATACTATAGGGGGGTATTCTATATGTCAATGCATAACCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAATCAAAGAGGAACGGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                                                               >  2:3800300/1‑150 (MQ=255)
                                        tctcCATGCAATATACTATAGGGGGTTATTCTAAATGTCAATGCATACCCCCCTATACTATATAACACTGGACAATAAAATTTAACCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                              <  2:1766134/150‑1 (MQ=255)
                                        gcTGCATGCAATATACGCGCGGGGGGTATTCGATCTGTCACTGCATCCCCCCCGAGAGCAGACACCCCGGGAGAATACAATTGCGCCGGTGAAGGTGGCCGGAAACCACCGCGGACCGGGGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                                                                              <  2:258468/149‑1 (MQ=255)
                                         cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTATTCGGCAGGGAAGGCCGcccc                                                                                                             >  2:5262152/1‑150 (MQ=255)
                                         cTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                                                                             >  1:1428358/1‑150 (MQ=255)
                                          tgcatgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCg                                                                                                            >  1:1574526/1‑150 (MQ=255)
                                              tgcaATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATa                                                                                                        >  1:1854973/1‑150 (MQ=255)
                                                 aaTATACTCTAGGGGGGTATGCTATATGTCACTGCATACCCCCCTATATTATATAACACTGGAGAATAAACTTTATCCGGTGAAGGTGGTCGGAAAACAACGAGGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                                                                     <  2:2159386/150‑1 (MQ=255)
                                                  ctacccTAGAGGGGGGTCTCCTATCTGGCAATGCATACCCCCCTATCGTATATAACCCTGGCGCCGAACCTTTAGCCGGTGACGGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                                    <  2:3344438/145‑1 (MQ=255)
                                                  atatACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgg                                                                                                    >  1:5287941/1‑150 (MQ=255)
                                                     taCTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                                                                 >  2:5585647/1‑150 (MQ=255)
                                                        tataGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                                                              >  2:2340799/1‑150 (MQ=255)
                                                          taGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGACAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                                                                            >  2:2153744/1‑150 (MQ=255)
                                                           aGGGGGGTACTCTCTATGTCAATGCATACCCCCCTATAGTATAGAACACTGGAGCACACCATGTATCCGGTGAAGGTGGCCGCAACCCCAAGCGGACAGGGGGGGGGGCCACTCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGaa                                                                                           <  2:3775652/150‑1 (MQ=255)
                                                             gggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAACATTTATCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGa                                                                                        <  2:4235943/150‑1 (MQ=255)
                                                               gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAAACGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                                                                       >  2:1183864/1‑150 (MQ=255)
                                                                ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                                                                      >  2:3422017/1‑150 (MQ=255)
                                                                 gTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                                                                     >  2:744454/1‑150 (MQ=255)
                                                                     tCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                                                                 >  2:1014284/1‑150 (MQ=255)
                                                                      cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                                                                >  1:2465757/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGACAACACAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAAtt                                                                               >  1:2349976/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAAtt                                                                               >  1:2501999/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                               >  1:2748672/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                               >  1:5311065/1‑150 (MQ=255)
                                                                       tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAtt                                                                               >  2:3270593/1‑150 (MQ=255)
                                                                         tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAACGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTc                                                                             >  2:1468507/1‑150 (MQ=255)
                                                                           tgacAATGCATCCCCCCCTATAGTATATAACACCGGAGAATAAAACCTCTCCGGTGACTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                                                                           <  2:285272/147‑1 (MQ=255)
                                                                            gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                                                          >  2:1243392/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                         >  2:4512302/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                         >  1:1293211/1‑150 (MQ=255)
                                                                             tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                                                                         >  1:5699538/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTc                                                                       >  1:2455313/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGACTTTCCAGGTc                                                                       >  2:5088103/1‑150 (MQ=255)
                                                                               aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                                                                       >  1:5028928/1‑150 (MQ=255)
                                                                                  gCATCCCCCCCTATAGTCCACACCACTGGCGCCTCACATCTACCCGGTGCACGTGGCCGGCAACCACCGAGGCAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCAGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                    <  2:100433/150‑1 (MQ=255)
                                                                                  gCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                                                                    >  1:137385/1‑150 (MQ=255)
                                                                                      aCCCCCCTTTAGTATATAACACGGGATTAGAAAATTTATCCTGTGAATGTGGGCCGAAAACACCGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                                                                <  2:356419/150‑1 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  2:234657/1‑150 (MQ=255)
                                                                                        cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                                                              >  1:5396129/1‑150 (MQ=255)
                                                                                           ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                                           >  2:2003105/1‑150 (MQ=255)
                                                                                           ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                                                           >  2:598011/1‑150 (MQ=255)
                                                                                             tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg                                                         >  2:1443127/1‑150 (MQ=255)
                                                                                                     ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc                                                 >  2:5658515/1‑150 (MQ=255)
                                                                                                         cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGtt                                             >  2:657808/1‑150 (MQ=255)
                                                                                                            tGGAGAATAAAATTTATCCGGTGAAGGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaa                                          >  2:4390114/1‑150 (MQ=255)
                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAACGGGGGGGGGGCTAATCGGCCGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa                                         >  2:5245223/1‑150 (MQ=255)
                                                                                                             ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa                                         >  2:4834913/1‑150 (MQ=255)
                                                                                                             ccagaATAAAAAGCAACCGGTGAAGGTGGTCGGAACACAAAGAGGAACGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTaaaa                                         <  2:3481587/148‑1 (MQ=255)
                                                                                                              gagaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAat                                        >  2:2198916/1‑150 (MQ=255)
                                                                                                                gaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAatca                                      >  1:298752/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGACTCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  1:4953638/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  2:5338776/1‑150 (MQ=255)
                                                                                                                   tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCg                                   >  2:2770103/1‑150 (MQ=255)
                                                                                                                     aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGt                                 >  2:766519/1‑150 (MQ=255)
                                                                                                                       aTTTATCCGGTGACTGTGGTCGGAATACAAAGAGGAAGGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATGTCCAGGTCCGACGGGCTGCACGTTGCAGACCGTTACACTCATCggggg                               >  2:855445/1‑147 (MQ=255)
                                                                                                                          tATCCGGTGAATGTGGTCGGAAAACACAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGgt                           <  2:4267370/150‑1 (MQ=255)
                                                                                                                          tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGGTgggg                            >  2:2035541/1‑150 (MQ=255)
                                                                                                                               ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCg                       >  1:3634396/1‑150 (MQ=255)
                                                                                                                                 tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTa                     >  1:5124808/1‑150 (MQ=255)
                                                                                                                                        ggTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACt              >  1:4732304/1‑150 (MQ=255)
                                                                                                                                        ggTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTAGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACt              >  1:5223506/1‑150 (MQ=255)
                                                                                                                                         gTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACtt             >  1:3575823/1‑150 (MQ=255)
                                                                                                                                           cGGAAAAAAAAGAGGAAAGGGGGGGGGGCTAATCGGAAGGGAAGGCCGCCCCGGATATCGGGCGGAAGAAGGAATCAGAATTTCCAGGACAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTa           >  2:3900199/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc          >  1:5756883/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTAc          >  2:1835797/1‑150 (MQ=255)
                                                                                                                                            ggAAAACAAAGAGGAAAGGGGGGGGGGCTAAAAGGCAGCGAAGGCCGCCCCGGATAGAGGTCGGCAGAACGAATAAGAATTTACAGTTCAGACGGGCAGCAAGTTGCAGACCGTTAAAAACATCTGTTCTGGTGTCGAACCAAACTTTAc          >  2:1230609/1‑150 (MQ=255)
                                                                                                                                                aaCAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAAACATCGGTTGGGGTGTCGAACCACACTTTACCTGc      >  2:3187006/1‑150 (MQ=255)
                                                                                                                                                  cAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg    >  1:4468116/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCg  >  2:4654005/1‑149 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGGCTAATAGGAAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGAAGACCGTTAAAATCATCGGTTTGGGTGTCGTACCACACTTTACCTGCCGt   >  1:4560768/1‑150 (MQ=255)
                                                                                                                                                   aaaGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  2:2700785/1‑150 (MQ=255)
                                                                                                                                                    aaGAGGAAAGGGGGTGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTc  >  2:3850725/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TTCTTCTCTTCCGGAGTACTGGGCATTTCGCACCTCATCATCTGCATGCAATATACTATAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTAAAATCATCGGTTGGGGTGTCGTACCACACTTTACCTGCCGTC  >  NC_000913/379856‑380153

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: