Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 708,015 T→A 7.8% intergenic (+258/‑319) glnS → / → chiP glutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913708,0150TA 7.8% 913.0 / 6.8 301intergenic (+258/‑319)glnS/chiPglutamyl‑tRNA synthetase/chitoporin, uptake of chitosugars
Reads supporting (aligned to +/- strand):  ref base T (90/177);  new base A (32/2);  total (122/179)
Fisher's exact test for biased strand distribution p-value = 4.55e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 313 total aligned reads displayed.

ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164
                                                                                                                                                     |                                                                                                                                                     
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAAtt                                                                                                                                                      <  1:901208/150‑1 (MQ=255)
 tGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAAtt                                                                                                                                                      >  2:2307847/1‑150 (MQ=255)
   aTCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGGGCTTTTATTCGCGAATTAATTACGCATAGCAATTTa                                                                                                                                                    >  1:5430675/1‑150 (MQ=37)
   aTCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGCTTTTATTGGCGAATTAATTACGCTTAGCAAATTa                                                                                                                                                    >  1:1530997/1‑150 (MQ=25)
    tctcTGCAATTTATTGTGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTAt                                                                                                                                                   <  1:5741847/150‑1 (MQ=255)
    tctcTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGATTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTAt                                                                                                                                                   <  2:3291780/150‑1 (MQ=255)
     ctctGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATc                                                                                                                                                  <  2:3709563/150‑1 (MQ=255)
      tctGCAATAGATGGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATcc                                                                                                                                                 <  2:5818265/150‑1 (MQ=255)
        tGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTGTTTCTGCTTTTCTTGGCGAATTAATTACGCGTCGCAATTTATCCaa                                                                                                                                               >  1:1740167/1‑149 (MQ=255)
            atatatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaa                                                                                                                                           >  1:3365418/1‑150 (MQ=255)
              atcttGAGTTTGCGTGCTTTTGTCGGCCGGATAAGGCGTTCACGCCGCATCCGGAAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaata                                                                                                                                         <  1:2168654/147‑1 (MQ=255)
              atatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaata                                                                                                                                         <  1:3884247/150‑1 (MQ=255)
              atatTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCataaaata                                                                                                                                         <  1:853572/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAaaataaat                                                                                                                                      <  1:1474435/150‑1 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATCCATAaaataaat                                                                                                                                      >  2:3091744/1‑150 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCATAaaataaat                                                                                                                                      >  1:469808/1‑150 (MQ=255)
                 ttGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTAGCAAATTATCCATAaaataaat                                                                                                                                      >  1:417053/1‑150 (MQ=255)
                  tGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTCAGCTTAGCAAATTATACATAAAATAAAtt                                                                                                                                     >  2:4876956/1‑150 (MQ=38)
                    aGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTa                                                                                                                                   <  2:1412623/150‑1 (MQ=255)
                      tttGCGTGCTTGTGTAGGCCGGATAAGGCGTGCACGCCTCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTaaa                                                                                                                                 <  2:4117625/150‑1 (MQ=255)
                          cGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTACGCATCGCAAATTATCCATAAAATAAATTTAAAATaa                                                                                                                             >  1:1901380/1‑150 (MQ=255)
                           gTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATaaa                                                                                                                            >  1:3188466/1‑149 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTATTAGCGAATTAATTCCGCTTAGCAATTTATCCATAAAATAAAATTAAAATAACa                                                                                                                           >  1:5128668/1‑150 (MQ=255)
                            tGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTATTCGCGAAATAAATACCCGTAGCAAATTATACAAAAAATAAAATTAAAATaaaa                                                                                                                           >  2:1499325/1‑148 (MQ=37)
                             gCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaa                                                                                                                          <  2:3988930/150‑1 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaaaa                                                                                                                        <  2:3234042/150‑1 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACaaaa                                                                                                                        <  2:3811015/150‑1 (MQ=255)
                               ttttGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAAATAATTACGCTTAGCAAATTATACAAAAAATAAATTTAAAATAaaaaaa                                                                                                                        >  1:5535871/1‑150 (MQ=38)
                                  tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTTCTTTTCTTAGCGAATTAATTACGCTTCGCAAATTATCCATAAAATAAATTTAAAAAAACAAAACAt                                                                                                                     >  1:876944/1‑150 (MQ=39)
                                  tGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACAt                                                                                                                     <  2:1886087/150‑1 (MQ=255)
                                     aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAAt                                                                                                                  <  2:5604869/150‑1 (MQ=255)
                                         cGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATtaaa                                                                                                              <  1:300266/150‑1 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAAAAAAAaataaataaata                                                                                                            >  2:639567/1‑150 (MQ=255)
                                           gATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTGTTTCTGCTTTTCTTGGCCAATTAAATAACCGACGCAAATATTACAAAAAATAAAATTAAAATAAAAAAACATAATaaaata                                                                                                            >  1:2786742/1‑150 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACTGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  2:3889158/150‑1 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  1:5029268/150‑1 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATaaaa                                                                                                         <  2:3323214/150‑1 (MQ=255)
                                              aaGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAAATCCGCTTCGCAAATTATACATAAAATAAATTTAAAATAACAAAACATAAATAAATaaaa                                                                                                         >  2:2143863/1‑150 (MQ=255)
                                               aGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGGTTTGTGCTTTTGTTCGCGAATTAATTACGCGTCGCAATTTTTACATAAAATAAATTTAAAATAAAAAAAAAAAATTAAATAAAAt                                                                                                        >  1:1284660/1‑150 (MQ=255)
                                                      cACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTGTTTTGTGGTTTTGTTGGCGCATTAATTCCGGGTCGCGATTTATACCAAAAAAAAAATTAAAATAAAAAAAAAAAATTAAATAAAAttttacag                                                                                                 >  1:2709585/1‑143 (MQ=255)
                                                            gcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCa                                                                                           <  2:1803675/150‑1 (MQ=255)
                                                            gcATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCa                                                                                           <  2:4812390/150‑1 (MQ=255)
                                                             cATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTCTTCGCGAATTAATTCCGCGTCGCAAATTATCCATAAAAAAAATTTAAAATAAAAAAACATAATTAAATAAAATTTAACCGCTTTAAt                                                                                          >  1:3325484/1‑150 (MQ=255)
                                                             cATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCAt                                                                                          <  2:2037978/150‑1 (MQ=255)
                                                                ccGGCAAGAAACAGCAAACAATCCAACACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAAGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCtt                                                                                       <  1:5705148/150‑1 (MQ=255)
                                                                ccGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCtt                                                                                       <  2:604115/150‑1 (MQ=255)
                                                                  ggCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGc                                                                                     <  1:4503375/150‑1 (MQ=255)
                                                                   gCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCt                                                                                    <  1:1854370/150‑1 (MQ=255)
                                                                    cAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTGTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAAAAAAAAATAAATAAATAAAATGTAACCGCTTTAATCTTGCTg                                                                                   >  2:256921/1‑150 (MQ=255)
                                                                       gAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTATGCTTTTCTTCGCGAATTAATTCCGCGTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATTTAACCGCTTTCATCTTGCGGGaa                                                                                >  2:5510808/1‑150 (MQ=255)
                                                                         aaCAGCAAACAATCCAAACCGCCGCTTTCAGCTGCTTTTTTTCTGCTTTTCTTCTCGATTTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAATATTTAACCGCTTTCATCTTGCTGGAAtt                                                                              <  1:2325521/150‑1 (MQ=255)
                                                                          aCAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAAttt                                                                             <  1:384845/150‑1 (MQ=255)
                                                                            accAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  1:4876956/148‑1 (MQ=255)
                                                                            aGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCa                                                                           <  2:5455542/150‑1 (MQ=255)
                                                                              cAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                         <  1:3827884/150‑1 (MQ=255)
                                                                              cAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACg                                                                         <  1:5320797/150‑1 (MQ=255)
                                                                                     tCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCGTTTCTTCGCGAAATAATTCCGCTTCGCAATTTATCCAAAAAATAAATTTAAAATAACAAAACAAAATTAAATAAAATGTAACCCCGTTCATCTTGCTGGAATGTCACGCGTTTAt                                                                  >  2:3748999/1‑150 (MQ=255)
                                                                                      ccAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAtt                                                                 <  2:2709585/150‑1 (MQ=255)
                                                                                       cAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttc                                                                >  2:1084474/1‑150 (MQ=255)
                                                                                       cAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAAAGTAACCGCTTTAATCTTGCTGGAATTTCACGCTGTTAtta                                                                >  2:4247557/1‑149 (MQ=255)
                                                                                        aaaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCCTAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCGTTCATCTTGCGGGAATTTAACGCGTTTAttat                                                               >  2:4500254/1‑148 (MQ=255)
                                                                                         aaaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttctt                                                              <  1:3710103/150‑1 (MQ=255)
                                                                                          aaCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTAttcttc                                                             <  2:4375557/150‑1 (MQ=255)
                                                                                           ccgccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATtcttct                                                            <  1:1386760/149‑1 (MQ=255)
                                                                                           aCGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTACGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATACAATGTAACCGCTTTCATCTTGCTGGAATTTCACTCTTTTATtcttct                                                            <  2:208597/150‑1 (MQ=255)
                                                                                             gccgcGTTCAGCGGCGTTTTTTCTGCTTTTCTTAGCGAATTAATTCCGCTTAGCAAATTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAAATTAACCGCGTTAATATTGCTGGAATTTCACGCGTTTATTCATCTGc                                                          >  2:5012655/1‑150 (MQ=255)
                                                                                             gccgcGTTCAGCGGCGTTTTTTCTGCTTTTCGTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATACTTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGc                                                          <  2:2981153/150‑1 (MQ=255)
                                                                                               cgcgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTTGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCaa                                                        <  1:2489989/150‑1 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTACGTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGc                                                      <  1:551573/150‑1 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGc                                                      <  1:4805905/150‑1 (MQ=255)
                                                                                                 cgTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGc                                                      <  1:4245040/150‑1 (MQ=255)
                                                                                                   ttCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCt                                                    <  2:2449791/150‑1 (MQ=255)
                                                                                                         ggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAAc                                              <  1:639567/150‑1 (MQ=255)
                                                                                                         ggcgTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAAc                                              <  1:441225/150‑1 (MQ=255)
                                                                                                            gTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGc                                           <  1:1833982/150‑1 (MQ=255)
                                                                                                                tttCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAAc                                       <  1:1338824/150‑1 (MQ=255)
                                                                                                                     gCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAg                                  >  2:5127062/1‑150 (MQ=255)
                                                                                                                         ttcttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCtt                              <  1:765834/150‑1 (MQ=255)
                                                                                                                           cttcGCGTATTAATTCCGCTTCGCTATTTATCCATAAAATAAATTTATAATAACAAAACATAATTAAATTAATTGTTACCGCTTTCATCTTGGTGGAATTTCACGCTTTTTTTCTTCTGCAAGCCTTTGAACCGCAAACTTAAGCCTTGt                            <  1:5659674/150‑1 (MQ=255)
                                                                                                                           cttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGt                            <  2:1256430/150‑1 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                           >  2:4446396/1‑150 (MQ=255)
                                                                                                                            ttcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTa                           >  1:3930591/1‑150 (MQ=255)
                                                                                                                             tcGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTaa                          >  2:4187741/1‑150 (MQ=255)
                                                                                                                               gcgAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACa                        >  2:5300520/1‑150 (MQ=255)
                                                                                                                               gcgAATTAATTCCGCTTCGCAATTAATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTGATTCTTCTGCAAGCCTTTCACCAGCAAACTTAATCCTTGTAACa                        >  2:3441316/1‑150 (MQ=255)
                                                                                                                                 gAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACaaa                      <  2:2793956/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   <  2:2283501/150‑1 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAat                   >  2:1180982/1‑150 (MQ=255)
                                                                                                                                    ttaattCCGCTTCGCAATTTAACCATAAAATAAAATTAAAAAAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTATCAACCGCAAACTTAAGCCTTGTAACAAAAat                   >  2:1300571/1‑150 (MQ=255)
                                                                                                                                       attCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcat                <  1:2574550/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  1:4949120/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               <  1:1084474/150‑1 (MQ=255)
                                                                                                                                        ttCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAatcatc               >  2:2918320/1‑150 (MQ=255)
                                                                                                                                         tCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAAtcatca              <  2:3561554/150‑1 (MQ=255)
                                                                                                                                              ttCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCACACTTAAGCCTTGTAACAAAAATCATCAAAata         >  2:351622/1‑150 (MQ=255)
                                                                                                                                                cGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtg       >  1:2869638/1‑150 (MQ=255)
                                                                                                                                                cGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtg       >  1:4104579/1‑150 (MQ=255)
                                                                                                                                                  cAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATAtgtg     >  2:4989039/1‑150 (MQ=255)
                                                                                                                                                     tttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCgg  <  1:2143863/150‑1 (MQ=255)
                                                                                                                                                     tttATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCgg  <  1:115180/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
ATGATCTCTGCAATATATTGAGTTTGCGTGCTTTTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAAGAAACAGCAAACAATCCAAAACGCCGCGTTCAGCGGCGTTTTTTCTGCTTTTCTTCGCGAATTAATTCCGCTTCGCAATTTATCCATAAAATAAATTTAAAATAACAAAACATAATTAAATAAAATGTAACCGCTTTCATCTTGCTGGAATTTCACGCTTTTATTCTTCTGCAAGCCTTTCAACCGCAAACTTAAGCCTTGTAACAAAAATCATCAAAATATGTGCGG  >  NC_000913/707866‑708164

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑