Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 164,581 A→C 24.5% intergenic (+47/‑149) hrpB → / → mrcB putative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913164,5810AC24.5% 206.0 / 42.0 162intergenic (+47/‑149)hrpB/mrcBputative ATP‑dependent helicase/fused glycosyl transferase and transpeptidase
Reads supporting (aligned to +/- strand):  ref base A (46/74);  new base C (9/33);  total (55/107)
Fisher's exact test for biased strand distribution p-value = 5.83e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 163 total aligned reads displayed.

CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA  >  NC_000913/164433‑164730
                                                                                                                                                      |                                                                                                                                                      
cGTGAGGTGCAAAAAGAGATGAAAGGGCGGTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCACTCCCTGGGGGAGAGGGCCGGGGTGAGGGCAt                                                                                                                                                       <  2:2972356/150‑1 (MQ=255)
 gTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATc                                                                                                                                                      <  2:2742876/150‑1 (MQ=255)
  tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                                     >  1:2325166/1‑150 (MQ=255)
  tGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCa                                                                                                                                                     <  2:550055/150‑1 (MQ=255)
     ggTGAAAAAAGAGTTTAACGGGCGTTATCACAAACTTGTCTGGCCGTACGACCCGGAAAATACTGCACCGACGCGACGTACTAAAAAGTTTTCTTAAGGTTGT‑TTTT‑CGCCCTCTCCCTGTGGGAGTGGGCCGGGGTGAGGGCCTCAgcg                                                                                                                                                  <  2:3758800/150‑1 (MQ=255)
      gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc                                                                                                                                                 >  2:186178/1‑150 (MQ=255)
      gTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGAATTAGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAgcgc                                                                                                                                                 >  2:186313/1‑150 (MQ=255)
        gCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAgcgcgc                                                                                                                                               >  2:164638/1‑150 (MQ=255)
                 tttgattgggCTTTATCCCAAACTTGTGTGGCCGTACGACCCGGAAAATACTGCACGGACGCTAGGGACTAAAAATTTTTCTTATGTTTGT‑TTTT‑GACCCTCTCCCTGTGGTAGTGGGCCGGGGTTAGGGCATCAGCGCGCACGTtcacc                                                                                                                                      <  2:1224463/143‑1 (MQ=255)
                  aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGtgggccc                                                                                                                                     >  2:2689018/1‑144 (MQ=255)
                  aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtcaccc                                                                                                                                     >  2:833604/1‑150 (MQ=255)
                  aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcgccc                                                                                                                                     >  1:5489693/1‑150 (MQ=255)
                  aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcaccc                                                                                                                                     >  2:4071548/1‑150 (MQ=255)
                  aTGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGGtcgacc                                                                                                                                     >  1:5146896/1‑146 (MQ=255)
                    gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTggacccac                                                                                                                                   >  2:591698/1‑148 (MQ=255)
                    gAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGtgacccac                                                                                                                                   >  2:1329106/1‑148 (MQ=255)
                    gAAACGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCACATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTgcacccgc                                                                                                                                   >  2:536576/1‑148 (MQ=255)
                        gggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAATTACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCTGCGCGCACGTtgagcctcaacc                                                                                                                               >  2:5309680/1‑150 (MQ=38)
                         ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTgcgccccccccca                                                                                                                              >  1:3574960/1‑149 (MQ=38)
                         ggCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCCGCGCGCACGTtcacccacacccc                                                                                                                              >  1:4716803/1‑149 (MQ=255)
                          gCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTtggcccacacccaa                                                                                                                             >  2:3997516/1‑148 (MQ=255)
                            gTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCCCGTTGGCCCGCCCCCGAAc                                                                                                                           >  1:4405240/1‑150 (MQ=38)
                             ttATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCACACCCAAAcc                                                                                                                          >  1:4790331/1‑150 (MQ=255)
                                cccaaaaCATGTCGGGCCGGACGACCCGGCAAATCCGGCACCGCCGCGCCGGACCAAAAAGTATTCTTATGTTTGT‑TTTT‑CCCCCCCACCCTGTGGTTGCGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCctc                                                                                                                       <  2:5397475/146‑1 (MQ=255)
                                 cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTGACCCGCAACCAAAaccaaa                                                                                                                      >  1:1375061/1‑147 (MQ=37)
                                 cccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCGCCCGCACCCCAAcccgca                                                                                                                      >  1:1921278/1‑147 (MQ=255)
                                  ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCCCACCCCAAaccaccc                                                                                                                     >  1:1985275/1‑146 (MQ=37)
                                  ccAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGGGCCCACACCCCAAACCactc                                                                                                                     >  1:1860633/1‑150 (MQ=37)
                                   cAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCAGCAGCGCGCACGTGGCCCCGCCCCCCAAcccacacc                                                                                                                    >  1:2553135/1‑145 (MQ=37)
                                    aaaCATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCACACCCAAACCCACAccc                                                                                                                   >  1:4295119/1‑150 (MQ=38)
                                        tggtggggccggccGCCCCGGAAAATACTGCCCCGCCGGGCCGTACTAAAATTTA‑TCGGTAGGTTGGTTTTT‑CTCCCCCCCCCTGTGGGTGTGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                               <  1:5458848/144‑1 (MQ=255)
                                        tggTCGGGCGGGACGCCCCGCCAATTCCGCCACCGCCGCGCCGGACTAAAAATTTTCCGAACGGTTTG‑TTTT‑CCCCCTCTCCCGGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                               <  1:2730386/148‑1 (MQ=255)
                                        aTGTCTGGCCGTCCGACGCGGCAATTCCTCCCCCGAGGCGCCGGACTAAAATTTTTTCGTATGGTTGG‑TTTT‑CTCCCTCCCCCTGGGGGTGTGGGCGGGGGGGAGGGCATCAGCGCGCCCGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                               <  2:1707298/150‑1 (MQ=255)
                                        aTGTCGGGCCGGACGCCCCGGCAAATCCTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCaa                                                                                                               <  2:1451090/150‑1 (MQ=255)
                                         ggacTGGCCGGACGACCCGTCAAATCCCGCACCTACGCGACGGACTAAAATGTATTCGTACGGTTGG‑TTTT‑CGCCCTCGCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAg                                                                                                              <  2:5118854/147‑1 (MQ=37)
                                            cTGGCCGGACGCCCCGCAAAATCCGGCACCGCCGCGCGGGACCAAAAAGTTTTCGTATGGTGGG‑TTTA‑CACCCTCCCCGGGTGGGAGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGga                                                                                                           <  1:5557738/150‑1 (MQ=255)
                                               gCCGGACGCCCCGGAAAATACTCCACCGCCGCGACGGACTAAAATTTATGCGTTTGGTTGT‑TTTA‑CTCCCTCTCGGTGTGGGTGTGGGCGGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGagag                                                                                                        <  1:1442046/150‑1 (MQ=255)
                                               gCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAATGTATTCGTAAGGGTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCCGCGCGCACGGGGGCCCGCGCCCCAAACCCCTCCCGCAAggggggg                                                                                                        >  1:5659862/1‑146 (MQ=25)
                                                ccGGACGCCCCGGCAAATACTGCACCGACGCGACGGACTAAAAAGTTTTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgg                                                                                                       <  2:3205856/150‑1 (MQ=38)
                                                 cGGCCGACCCGCAAAATCCCCCACCGCCGCCCCGGACTAAAATTTAGGCTTCGGTTTGT‑TTTT‑CCCCCTCCCCGTGGGGGTGGGGGCGGGGTTGCGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                      <  1:4461405/150‑1 (MQ=255)
                                                 cGGACGACCCGGCAATTACGGCACCGACGCGACGGACGAAAATGTATTCGTATGGTTGG‑TTTT‑CTCCCTCTCCGTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                      <  2:2392270/150‑1 (MQ=39)
                                                 cGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTACGGTTGG‑TTTT‑CCCCCTCTCCCTGTGGTAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAggg                                                                                                      <  2:3256012/150‑1 (MQ=255)
                                                  ggACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCGCAGCGCGCACGGGGACGCCCACCCCCAACCACACCCTCAAGGGGGTgggg                                                                                                     >  1:1217073/1‑150 (MQ=25)
                                                      gccccGCCAAATCCTGCACCGACGCGCCGGACGAAAAAGTTTTCGTATGGGTGG‑TTGT‑CCCCCGCCCCCTGGGGGTGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTTGAGGGAGAgggttagg                                                                                                 <  2:4033996/148‑6 (MQ=255)
                                                        cccgcaaaaTACTCCCCCTACGCCACTGAAGAAAATTTTTTCTTACGTTTTT‑TTTT‑CCCCCTCCCCGTGTGGTTGTGGGCGGGTGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGAt                                                                                               <  2:259604/144‑1 (MQ=255)
                                                         ccGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGTGCCCCGCACCCGAACCCACACCCACAACGGAGAGGGGccccacc                                                                                              >  2:3183157/1‑146 (MQ=25)
                                                         ccGGCAAAAACTGCACCGCCGCGACGCACTAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCCCCCTGTGGGAGCGGGCGGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATc                                                                                              <  2:4974867/150‑1 (MQ=255)
                                                           gcaaaaTACGCCACGGACGCTACGGCATAAAATTTTTTCGTTTGGTTGT‑TTTT‑CCCCCACTCCCTGTGGGTTTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGa                                                                                            <  1:1341816/147‑1 (MQ=255)
                                                            tcAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGT‑TTTT‑CTCCCACTCCCTGTGGGAGTGGGCCGGGGTGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                                           <  2:1981728/149‑1 (MQ=255)
                                                            gCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAg                                                                                           <  2:5789179/150‑1 (MQ=255)
                                                              aattACTGCACCGACGCGACGGACTAAAAAGTTTTCGTATGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGTGTGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                         <  2:3054323/147‑1 (MQ=38)
                                                              aaaTTCTCCCCCGACGCGCCGGACGAAAATGTTTTGGTTTGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGTTGTGGGCGGGGGTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                         <  2:3109855/150‑1 (MQ=255)
                                                              aaaTACTGCACCTCCGCGACGTACTAAAATTTTTTCTTTTGTTTGT‑TTTT‑CTCCCTCCCCGTGTGGTTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGca                                                                                         <  2:155006/150‑1 (MQ=255)
                                                                aTACTGCACCGACGCGACGGACGAAAAAGTATTCGGAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTGCACCCGCACCCGAACCCACTCCCACAAGGGGGCGGGGCCCGCTCGAGcaca                                                                                       >  2:3325239/1‑150 (MQ=37)
                                                                      cACCGCCGCGCCGCACCAAAACTTATTCTAATGGTTGT‑TTTT‑CCCCCTCCCGCTGTGGTTGAGGGCGGGGTTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AAtttt                                                                                <  1:3158459/150‑1 (MQ=255)
                                                                      cACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCGAACCCGAAAACACTACCAGAAGGGAGAGGGGGCCGAGACAGCACa‑aaaatt                                                                                >  1:4717349/1‑146 (MQ=25)
                                                                        ccGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCCTCAGCGCGCACGTGGGCCCCCACCCAAACCCCCACCCACCCCGGGGAGGGGGGCGGTCGAGGACACAAttttt                                                                               >  1:587035/1‑149 (MQ=16)
                                                                            cgcgaggGCCGAAAATTTTTGCGTAAGGTTGG‑TTTG‑CCCCCACCCCGGGTGGGTGGGGGCGGGGGGGCGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTgagaga                                                                          <  1:2226810/150‑1 (MQ=255)
                                                                                acggacgAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAACCCGCGCCCGCCAGGGCGAGGGGTCCGATCGAGCCCA‑AAATTTTATAGatata                                                                      >  2:3872628/1‑149 (MQ=25)
                                                                                  gtactaaaaaGTATTCGTATGGTTGG‑TTTC‑CTCCCTCTCCGTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtctt                                                                    <  1:862394/145‑1 (MQ=255)
                                                                                  ggacgAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCAACCACACCCAAAACCAAACCCACAAGGGAGAGGGGCCCGAAAGAGCCCA‑AAATTTTAGAAataaaat                                                                    >  2:5681089/1‑146 (MQ=21)
                                                                                   gacgAAAATGTAGTCGTAAGGTTGG‑TTTT‑CGCCCTCTCCCTGTGGGTGAGGGCCGGGGGGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttc                                                                   <  2:3617162/150‑1 (MQ=255)
                                                                                     ctaaaaaGTATTCGTATGGTTGG‑TTTT‑CTCCCTCTCCCGGTGGGAGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttctt                                                                 <  1:3359166/148‑1 (MQ=255)
                                                                                     cgAAAATTTATTCTTATGGTTGT‑TTTT‑CTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttctt                                                                 <  2:1628998/150‑1 (MQ=38)
                                                                                     cgAAAAATTTTTCGTATGGTTGT‑TTTT‑CTCCCTCTCCCTGTGGGTGCGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttctt                                                                 <  1:1870947/150‑1 (MQ=38)
                                                                                     cgAAAAAGTTTTGGTCCGGTTGG‑TGTT‑CTCCCTCTCCCGGGGGGAGTGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttctt                                                                 <  1:5219142/150‑1 (MQ=255)
                                                                                       aaaaaTTATTCGTTTGTTTGG‑TTTT‑CCCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATAtcttcttct                                                               <  1:1803403/150‑1 (MQ=38)
                                                                                          aaGTATTCGTTAGGTTGG‑TTTT‑CTCCCTCGCCCTGTGGGAGAGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑ATTTTTGAGAGATATCTTCTTCTGTc                                                            <  2:3910642/150‑1 (MQ=255)
                                                                                            gttttCGTATGGTTGG‑TTTT‑CGCCCTCGCGCTGGGGGAGTGGGCGGGGGTGTGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCtt                                                          <  2:5655994/147‑1 (MQ=255)
                                                                                            gTATGCGTACGGTTGG‑TTGT‑CACCCACCCCCTGTGGGAGTGGGCCGGGTTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCGTCTGTCtt                                                          <  1:1264376/150‑1 (MQ=255)
                                                                                             ttttggTATGGTTGT‑TTTG‑CGCCCTCCCCCTGTGGGAGCGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTg                                                         <  2:282739/145‑1 (MQ=255)
                                                                                               ttCTTACGGTTGT‑TTTT‑CTCCCTCTCCCTGTGGGTGTGGGCGGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTa                                                       <  1:708364/150‑1 (MQ=39)
                                                                                                  gTACGGTTGG‑TGGT‑CGCCCTCGCCCGGGGGGCGCGGGCCGGGGGGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTGCTTCTGTCTTGTAACa                                                    <  1:880018/150‑1 (MQ=255)
                                                                                                     aGGTTTG‑TTTTGCCCCCGCCCCGTGGGGGTGGGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACaga                                                  <  2:4405240/150‑1 (MQ=255)
                                                                                                          gg‑ttTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGGGCCCCCCCCCCCTAAACCCCTCCCTCCAGGGCGAGGGGACCGCTCGAGCACA‑AATTATGAGAGGTATATTCTTATGTCTTGTGACcgaagtaaa                                            >  2:5630418/1‑146 (MQ=21)
                                                                                                          gg‑ttTT‑CTCCCTCTCCCTGGGGGAGAGGGCCGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACa                                            <  2:4841610/150‑1 (MQ=255)
                                                                                                                   gccctcgccctGTGGGAGTGGGCGGGGGTGCGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                                     <  2:2325166/149‑1 (MQ=255)
                                                                                                                   gccccccccctGTGGGAGAGGGCGGGGGGGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATc                                     <  2:1375061/143‑1 (MQ=255)
                                                                                                                     cctctccctGTGGGAGAGGGCCGGGGTGTGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGATAATCgg                                   <  2:305583/150‑1 (MQ=255)
                                                                                                                           tctGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCtttt                             <  1:5555298/149‑1 (MQ=255)
                                                                                                                                 gtagtgggCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAGCCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                       <  2:4905413/145‑1 (MQ=255)
                                                                                                                                 ggtgtgggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCt                       <  1:2343306/145‑1 (MQ=255)
                                                                                                                                 ggcgcgggCGGGGGTGCGGGCCGCAGCGCGCACGTTCACCCTCACCCGACCCCTCTCCCTCACGGGAGAGGGGACCGATCGAGCACA‑AATGTTGCGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAACTCGGGCTTGTGCGCCt                       <  2:1265329/145‑1 (MQ=255)
                                                                                                                                    gagGGCCGGGGTGAGGGCATCAGCGCGCACGTGGACCCCCACCCAAAACCACACCCACAAGGGAGAGGGGGCCGATGCAGCACA‑AAATTTTTGATTTATATTCTTCTGTGTTGTTACAGAAGAACACAAAATCGGGCTTTTGAGCCGGaa                    >  2:2436275/1‑150 (MQ=255)
                                                                                                                                      gggCGGGGGTGCGGCCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata                  <  1:3997516/150‑1 (MQ=255)
                                                                                                                                      gggCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAata                  <  2:1935476/150‑1 (MQ=255)
                                                                                                                                         ccGGGGTGAGGGCCTCCGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAatatgg               <  1:5471772/150‑3 (MQ=255)
                                                                                                                                          cGGGGTGAGGCCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGc              <  2:4482777/150‑1 (MQ=255)
                                                                                                                                           ggggggTGGGCCTCAGCGCGCACGTTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg             <  1:2065735/150‑1 (MQ=255)
                                                                                                                                           ggggTGGGGGCCTCAGCGCGCACGGTCACCCTCACCCGAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg             <  1:2065754/150‑1 (MQ=255)
                                                                                                                                           ggggTGAGGGCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg             <  1:4569226/150‑1 (MQ=255)
                                                                                                                                           ggggTGAGGGCATCAGCGCGCACGTTCACCCTCACGCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCg             <  1:4194647/150‑1 (MQ=255)
                                                                                                                                           ggggTGAGGGCATCAGCGCGCACGTTCACCCACACCCAAAACCACACCCACAAGGGCGAGGGGACCCCACGAGCACA‑AAATTTTAGAGATATATTCTTAAGTCATTTAAAAGAAGAACAGAAAAAAGGGCGTTTGCGGCTGAATAtttag             >  2:2461675/1‑147 (MQ=255)
                                                                                                                                             ggTGAGGGCCTCAGCGCGCACGGTCACCCTCACCCTAACCCTCGCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga           <  2:3643184/150‑1 (MQ=255)
                                                                                                                                             ggTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGga           <  2:4727020/150‑1 (MQ=255)
                                                                                                                                                 tgggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCTAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaa       <  1:536576/149‑1 (MQ=255)
                                                                                                                                                  gggCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaa      <  1:5763059/150‑1 (MQ=255)
                                                                                                                                                   gtcATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACG‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa     <  1:418633/148‑1 (MQ=255)
                                                                                                                                                   ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa     <  1:4860456/150‑1 (MQ=255)
                                                                                                                                                   ggCCTCAGCGCGCACGTTCACCCTCACCCTAACCCACTCCCGCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTACTGTCTTGTACCAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGaaaaa     <  2:3432995/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                      
CGTGAGGTGCAAAAAGAGATGAAAGGGCGTTATCCCAAACATGTCTGGCCGGACGACCCGGCAAATACTGCACCGACGCGACGGACGAAAAAGTATTCGTAAGGTTGG‑TTTT‑CTCCCTCTCCCTGTGGGAGAGGGCCGGGGTGAGGGCATCAGCGCGCACGTTCACCCTCACCCTAACCCTCTCCCTCAAGGGAGAGGGGACCGATCGAGCACA‑AATTTTGAGAGATATCTTCTTCTGTCTTGTAACAGAAGAACAGAAAATCGGGCTTTTGCGCCTGAATATTGCGGAGAAAAAGCA  >  NC_000913/164433‑164730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑