Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,287,149 G→A 6.9% noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR ←
tpr ←
rtT sRNA, processed from tyrT transcript
protamine‑like protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,287,1490GA 6.9% 619.2 / 27.4 222noncoding (88/171 nt)
R10* (CGA→TGA) 
rttR
tpr
rtT sRNA, processed from tyrT transcript
protamine‑like protein
Reads supporting (aligned to +/- strand):  ref base G (94/109);  new base A (8/7);  total (104/118)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298
                                                                                                                                                    |                                                                                                                                                      
ttCCCCGCATGGCCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                       <  1:5750577/150‑1 (MQ=255)
    ccGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGACGTTGCCTGCGGCAACGCTCTCTCGCTTTCGCTCGAATCg                                                                                                                                                   >  2:1968193/1‑150 (MQ=255)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCTGCGGCGACGCTCTCGCGGAGGCGCGCGCGGCGCACCGTgg                                                                                                                                           >  1:2067572/1‑150 (MQ=14)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTgg                                                                                                                                           >  2:4304446/1‑150 (MQ=255)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTgg                                                                                                                                           <  1:929135/150‑1 (MQ=255)
            gCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCCAGTCGAACCGTgg                                                                                                                                           >  1:5675178/1‑150 (MQ=255)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCCACCCACACTCGCAGGCGCGCGAGTCGAAccgaggt                                                                                                                                          >  1:2113059/1‑145 (MQ=14)
             cAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGGCGTTTCCTGCGGCAACGATCTCTCGCTGTCGCTCGAGTCGAAACTTGGt                                                                                                                                          >  1:1792895/1‑150 (MQ=14)
              aGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTc                                                                                                                                         <  1:4122742/150‑1 (MQ=255)
                 atatTTGATTGCGGATTCGCTTGAGAGTTCAGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                      <  2:5065735/150‑1 (MQ=255)
                 atagTTGATTGCGGCTTCGCTTGAGATTTCCGGGTCTTTTGAAAGTGATGGTGGTGGTGGAAGGATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGAAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGaa                                                                                                                                      <  1:5409918/150‑1 (MQ=11)
                  tatTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTTCCTGCGGCAACGCACTCACGCTGGCGCACGAGTCGAAACATGGTCGAAg                                                                                                                                     >  1:277358/1‑150 (MQ=14)
                   atTTGATTGCGGATTCGCGTGAGAGTTCCGGGTCTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGc                                                                                                                                    <  1:5470462/150‑1 (MQ=255)
                        cttGCTGATTCGCTTGAGAGTTCAGGGACTTTAGCAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                               <  1:3924048/149‑1 (MQ=255)
                        aTTGCGGATTCGCTTGAGAGTTCCGGGCCTTTTGAACGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACTCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                               <  2:5690463/150‑1 (MQ=255)
                             ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCt                                                                                                                          >  2:2435606/1‑150 (MQ=255)
                             ggATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAAACTTGGTAGAAGCTTCTAATCCt                                                                                                                          >  2:4393431/1‑150 (MQ=255)
                                ttCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCACTCTCGCGGGCGCTCGAGTCGAACCTTGGTCGAAGATTATCAACCGTcc                                                                                                                       >  1:4502835/1‑150 (MQ=14)
                                  cGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTTCGCTCGAGTCGAACCTTAGTCGAAGCATCTCATAATTcccc                                                                                                                     >  2:4765496/1‑150 (MQ=14)
                                  cGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTcccc                                                                                                                     <  2:1844370/150‑1 (MQ=255)
                                      tGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCGCGCTGGCGCTCGAGTCGAACCATGGTCGCAGCTTATCATCCATCCCCGCag                                                                                                                 >  1:2081329/1‑149 (MQ=14)
                                              ccgggTCTTTTGACAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCATCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGaa                                                                                                         <  1:1909585/148‑1 (MQ=255)
                                                  gACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTGGCCGGCGGCAACCCACACACGCAGGCGCGAGCGTCGAAACAGGGTCGAAGCATATCAACCATCACCCCAAGGGCAGaaaaat                                                                                                     >  1:2025833/1‑146 (MQ=2)
                                                     ttttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCGGGCGCTCGAGTCGAACCATTGTCGCAGCGTCTAAAACATCCCCGCACGGGCCGAAAATTTaa                                                                                                 >  1:5103562/1‑150 (MQ=11)
                                                      tttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACCCAAGCTCGCGGGCCCACGAGTCGCAACATGGTCGAAGCGTCTAAACCATACCCCCAGGGGCAGGAAATGTAAt                                                                                                >  1:4445281/1‑150 (MQ=2)
                                                       ttGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAAtt                                                                                               >  2:4711222/1‑150 (MQ=39)
                                                            aGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGa                                                                                          <  1:2164871/150‑1 (MQ=39)
                                                                   gtggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGTGTCGAACCTTGGTCGTAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTg                                                                                   <  1:253224/150‑1 (MQ=255)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGGCGGGGGCAACCCACACACGCGTGCGCGCGCGTCGCAACATGGTCGAAGCTTATCATCCATCCCCGCAAGGGCAGAAAAATTAAGTGCGGAGTCGGTgg                                                                                  >  1:3179800/1‑150 (MQ=2)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                       >  2:1112777/1‑82 (MQ=32)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGa                                                                                                                                                       <  1:1112777/82‑1 (MQ=32)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  2:4331976/150‑1 (MQ=38)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  1:3298217/150‑1 (MQ=38)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTgg                                                                                  <  1:4597447/150‑1 (MQ=38)
                                                                    tggtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCACATCCTTCCCCGCATGGGCAGAATATTTAATTTCGGATTCGTTgg                                                                                  >  2:638088/1‑150 (MQ=255)
                                                                      gtgtGGGATGGATTTATCAGCGCTGCGCGGTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                <  1:5037411/150‑1 (MQ=14)
                                                                      gtggGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGa                                                                                <  1:600701/150‑1 (MQ=40)
                                                                           ggAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCtt                                                                                                                                  >  2:3610458/1‑96 (MQ=33)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCa                                                                          <  2:3576418/150‑1 (MQ=255)
                                                                            gAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGACGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGCTTGGCAGTTCa                                                                          >  2:3832098/1‑150 (MQ=11)
                                                                             aaGGATTACTCAGCGCTGCGCGCTTCGCCCTTCTGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:1624510/150‑1 (MQ=255)
                                                                              cggATTACTCCGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  1:3534621/149‑1 (MQ=255)
                                                                              aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAgg                                                                        <  1:2653327/150‑1 (MQ=255)
                                                                              aGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAg                                                                         <  1:959136/149‑1 (MQ=255)
                                                                               ggATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCGGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGGCGAAAATTTAATAGCGGAAGCGTTGGGTAGGTAAggg                                                                       >  2:4467941/1‑150 (MQ=14)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                      <  1:4711196/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGa                                                                      <  1:4711222/150‑1 (MQ=255)
                                                                                gATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCCTGGGCAGCATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGa                                                                      >  2:4668417/1‑150 (MQ=255)
                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGTCGCTCGAGTCGAACCTTTGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACt                                                                    <  1:4900944/150‑1 (MQ=255)
                                                                                  ttACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGCATGGGCCGAATATTTAATTGCGGAGGCGTTGGGGAGTTCAGGGAcg                                                                    >  2:3069437/1‑149 (MQ=14)
                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                   <  2:2416852/150‑1 (MQ=255)
                                                                                   tACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCGCGAGCCGCACCTTGGTCGAAGCTTATCATCCATCCCCCCAGGGGCCGCATATTTGATTGCGGGTTCGGGGGGGAGTTCAGGGACtt                                                                   >  2:3440245/1‑150 (MQ=11)
                                                                                     cTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtttt                                                                 <  1:833657/150‑1 (MQ=255)
                                                                                      tCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCGTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTg                                                                >  2:5168868/1‑150 (MQ=255)
                                                                                       cAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGa                                                               <  1:237430/150‑1 (MQ=255)
                                                                                        aGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCATCCCCGCATGGGCCGAATAGTGAACTGCGGCTTCGTTGGGACGGTCAGGGACGGTTGac                                                              >  2:2724487/1‑149 (MQ=14)
                                                                                          cgcTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTTGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCAACCTTCCCCGAATGGGCAGAAAATTAAATTTCGGAATCGTTTTGAAGTTCAGGGAGTTTTTaaat                                                            >  2:3690826/1‑149 (MQ=14)
                                                                                           gccgcgcgcTTCGCCCTTCGGGTCGGTGCCTGCGGCACCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGt                                                           <  2:3978034/147‑1 (MQ=255)
                                                                                             tGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:4757582/150‑1 (MQ=255)
                                                                                             ggcgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGa                                                         <  1:5658708/149‑1 (MQ=255)
                                                                                               cgcgcTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtg                                                       <  1:3857783/150‑1 (MQ=255)
                                                                                                 ctattCGCCCTTCGGGTCGTTGCCTGAGGCAACTCTCTCTCGCTGGCGCTCGAGTCGTACCTTGGTATATGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGAtggt                                                     <  1:5216222/147‑1 (MQ=14)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAGGGGCAGAAGATTTAATTGCGGAGTCGGTGGGAAGGTCAGGGACTGTTGAAAGTGCtggtggt                                                  >  2:3162553/1‑150 (MQ=14)
                                                                                                    ttCGCCCTTCGGGTCGTTGCCGGCGGCAACACTCTCTCGCTGGCGCTCGAGTCGAACCGTGGTCGAAGCTTCTCAACCTTCCCCCCATGGGCCGAATATTTAATTGCGGATTAGTTGGGAAGTTCAGGGGCGTTTGTGAGTGAtggtggt                                                  >  2:4568779/1‑150 (MQ=14)
                                                                                                     tCGCCCTTCGGGTCGTGGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTGTATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGAtggtggtg                                                 <  1:409224/150‑1 (MQ=255)
                                                                                                      cGCCCTTCGGGTCGTTGCCTGCGGCAACGCGCTCTCGCAGGCGCTCGAGTCGAACCATGGTCGTAGCGTCACATCCATCCCCGCAGGGGCAGAATATTTAAATGCGGATTCGGTGGGGAGTTCAGGGACGTTTGTAAGTGTtggtggggg                                                >  2:1192024/1‑150 (MQ=14)
                                                                                                          cTTCGGGTCGGTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGTATATTTTATTGCGGATTCGTTGGGTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGa                                            <  2:4923490/150‑1 (MQ=255)
                                                                                                            tCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          <  1:4680431/150‑1 (MQ=255)
                                                                                                              gggTCGTTGCCTGCGGCAAAGCTCTATCGCAGGCGCTCGAGGCGAACCATAGTCGAAGCATCACAAACATAACCCCAATGGCAGAATAATTAAATTCCGAATACGTTGGAACTTCAGGGACTTTTTAAAATGATGGTGGTGGGGGAAGGa                                        >  2:3002824/1‑150 (MQ=2)
                                                                                                                   gTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACtt                                                                   >  2:5660309/1‑118 (MQ=255)
                                                                                                                    ttGCCTGCGGCAACGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAg                                          <  1:3890798/142‑1 (MQ=255)
                                                                                                                       ccTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTTATGGTGGTGGGGGCAGGATTCGAAcca                               >  1:3756743/1‑149 (MQ=255)
                                                                                                                        cTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCtt                              <  1:4495567/150‑1 (MQ=255)
                                                                                                                         tGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTtctc                                                                                                                               <  2:477599/53‑1 (MQ=35)
                                                                                                                         atctgCAACGCTCTCTCGCTGGCGCTCTAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTTGTTAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTc                             <  1:1608218/146‑1 (MQ=14)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                            >  2:5766606/1‑150 (MQ=255)
                                                                                                                          gCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGAGTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCg                            >  2:5370548/1‑150 (MQ=255)
                                                                                                                             gCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAg                         >  2:5500761/1‑150 (MQ=255)
                                                                                                                              cAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCAt                                                                                                                 <  1:4362973/62‑1 (MQ=39)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       <  2:5229399/86‑1 (MQ=18)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       >  2:1925140/1‑86 (MQ=18)
                                                                                                                               aaCGCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTc                                                                                       >  1:5229399/1‑86 (MQ=18)
                                                                                                                                aCGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCg                      <  2:4502835/150‑1 (MQ=255)
                                                                                                                                 cGCTCTCTCTCGGGCGCACGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTTATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGa                     <  1:2952838/150‑1 (MQ=18)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    <  2:3622337/86‑1 (MQ=18)
                                                                                                                                  gCTCTCTCGCTGGCGCTCAAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGtt                                                                                    >  1:3622252/1‑86 (MQ=18)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                <  1:4568779/150‑1 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                <  1:3582310/150‑1 (MQ=255)
                                                                                                                                      tctcGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACg                <  1:4387321/150‑1 (MQ=255)
                                                                                                                                           cTGGCGCTCGCGTCGACCCTGGGTCGTAGCTTCTCATCCGGCCCCGCTTGGGCAGAATATTTCATGGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGGGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGa           <  1:1219342/150‑1 (MQ=18)
                                                                                                                                            tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          >  1:2542287/1‑150 (MQ=255)
                                                                                                                                            tGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGAt          >  2:740214/1‑150 (MQ=255)
                                                                                                                                              gcgcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAAGATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAACGTGATGGTGGTGGGGGTAGGATTCGAACCTTCGAAGTCGATGACGGCAGAttt        >  1:1483854/1‑150 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTAc      >  2:2130089/1‑150 (MQ=255)
                                                                                                                                                gcTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAACTTCGAAGTCGATGACGGCAGATTTaa      >  2:4005241/1‑149 (MQ=255)
                                                                                                                                                 gtCGATTCTAACCTTGGTAGAAGCGACTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGTAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:4001047/149‑1 (MQ=21)
                                                                                                                                                 cTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGTCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACa     <  1:1240054/150‑1 (MQ=255)
                                                                                                                                                  tCGAGCCGAACCTTAGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGGTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAAACTTCGAAGTCGATCACGGCACGGTTACAg    >  2:592671/1‑150 (MQ=17)
                                                                                                                                                   cGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGt   <  1:4304446/150‑1 (MQ=255)
                                                                                                                                                    gAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTc  <  2:4107373/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                      
TTCCCCGCATGGGCAGAATATTTGATTGCGGATTCGCTTGAGAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTACTCAGCGCTGCGCGCTTCGCCCTTCGGGTCGTTGCCTGCGGCAACGCTCTCTCGCTGGCGCTCGAGTCGAACCTTGGTCGAAGCTTCTCATCCTTCCCCGCATGGGCAGAATATTTAATTGCGGATTCGTTGGGAAGTTCAGGGACTTTTGAAAGTGATGGTGGTGGGGGAAGGATTCGAACCTTCGAAGTCGATGACGGCAGATTTACAGTC  >  NC_000913/1287001‑1287298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: