Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,680 T→C 8.4% intergenic (+287/+428) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,6800TC 8.4% 359.8 / 16.0 147intergenic (+287/+428)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (40/89);  new base C (2/10);  total (47/100)
Fisher's exact test for biased strand distribution p-value = 5.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 155 total aligned reads displayed.

TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829
                                                                                                                                                     |                                                                                                                                                     
tAGGTCAGACTTGGCGGTCACGCCTCATCCTACATACTTCGCCCGAGCCGGTTGCCTGATGCGACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                       <  1:4721930/150‑1 (MQ=14)
tAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCt                                                                                                                                                       <  1:4132680/150‑1 (MQ=255)
  ggTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                                                     <  1:509553/150‑1 (MQ=40)
  cgTCAGACCAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGTTGCGACGGTGTCGAGTCTTATGAGGCCTCCACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTaa                                                                                                                                                     <  1:4993757/149‑1 (MQ=11)
   gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAAc                                                                                                                                                    >  1:916326/1‑150 (MQ=40)
   gTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCCACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                                                                                                                                    <  1:4671956/150‑1 (MQ=255)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   <  1:99657/150‑1 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   <  2:5504656/150‑1 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACg                                                                                                                                                   <  1:4223729/150‑1 (MQ=39)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACg                                                                                                                                                   >  1:4888349/1‑150 (MQ=255)
    tCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGACGGATAAGGCGGGTACGCCGCATCCGACATCTAACg                                                                                                                                                   >  1:3753714/1‑150 (MQ=255)
     cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCTTTGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                                                  <  1:94122/150‑1 (MQ=255)
     cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                                                  <  2:4313515/150‑1 (MQ=38)
     cAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGc                                                                                                                                                  <  2:3610144/150‑1 (MQ=38)
      aGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGAAATCAAACGcc                                                                                                                                                 >  1:4139977/1‑150 (MQ=255)
       gACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGccc                                                                                                                                                >  2:4124184/1‑150 (MQ=37)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          <  1:1352037/150‑1 (MQ=21)
             gCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          <  1:4591387/150‑1 (MQ=34)
             gCGGTCACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCg                                                                                                                                          >  2:3803881/1‑150 (MQ=17)
              cGGTCACGCCTCTTCCGACATCCTACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCgg                                                                                                                                         <  1:3052897/150‑1 (MQ=255)
                gtaaCGCCGCCTCCGACATCCACCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCTTCTTATCAGGAATACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  2:237931/147‑1 (MQ=11)
                gTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGtt                                                                                                                                       <  1:5424312/150‑1 (MQ=34)
                 ttgggccgcACCGGACATCCAACGCCCCAGCCGGTGGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      <  1:2700718/146‑1 (MQ=11)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      <  1:700956/150‑1 (MQ=33)
                 tCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTg                                                                                                                                      <  1:3510801/150‑1 (MQ=33)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  1:3168565/150‑1 (MQ=32)
                  cACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGc                                                                                                                                     <  1:2700984/150‑1 (MQ=32)
                   aCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTTAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTACACCCCGTAGGTCGGATAAGGCGGGTACGCCGCATCCGACAACTAACGCCCAAGCCgggttcc                                                                                                                                    >  1:1463248/1‑145 (MQ=11)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGGTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCCCCCCGTAGGTCGGATAAGGCGGTTACGCCGCCTCCGACATCGAACGCCCAACCCGGTTGCCAGa                                                                                                                                 >  1:110611/1‑150 (MQ=11)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:5740524/150‑1 (MQ=32)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  1:3101037/150‑1 (MQ=32)
                      ccgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGa                                                                                                                                 <  2:2904838/150‑1 (MQ=32)
                       cgcATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGAt                                                                                                                                <  1:4886991/150‑1 (MQ=17)
                          aTCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCg                                                                                                                             >  1:3603574/1‑150 (MQ=32)
                           tCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGa                                                                                                                            <  1:5518685/150‑1 (MQ=32)
                            ccGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGAc                                                                                                                           <  2:3616454/150‑1 (MQ=17)
                             cGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                          <  2:1733409/150‑1 (MQ=32)
                             cGACATCCAACGCCCGAGCCGGTTGCCAGATGCGACGCTGGCGCGTCTTATAAGGCCAACACCCCAGTGAAGTGCTCCCCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACAAATAACGCCCAAGCCGGTTGCCTGATGCGACg                                                                                                                          >  1:5074330/1‑150 (MQ=11)
                              gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                         >  2:4923994/1‑150 (MQ=32)
                              gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                         >  2:1051282/1‑150 (MQ=32)
                              gACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGc                                                                                                                         >  2:3040848/1‑150 (MQ=32)
                               aCATCCAACGCCCGCGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCGACACCTCTGTGAAGTGCTGCACCCCGTAGGTAGGACAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCt                                                                                                                        <  1:2073581/150‑1 (MQ=11)
                                 aTCCAACGCCCGAGCCGGTTGCCTGCTGCGCCGCTGGCGCGTCTTATCAGCCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCACGCCGGTTGCCTGATGCGACGCTgg                                                                                                                      <  1:3863846/150‑1 (MQ=255)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  2:828544/150‑1 (MQ=32)
                                    cAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCGCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                                                   <  2:5280706/150‑1 (MQ=17)
                                     aaCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGGCTGATGCGCGGCTGGcgcg                                                                                                                  >  2:2241307/1‑150 (MQ=255)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  1:1743074/150‑1 (MQ=32)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  1:5234295/150‑1 (MQ=32)
                                      aCGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                                                 <  2:2703004/150‑1 (MQ=32)
                                       cGCCCGAGCCGGTTGCCTGCTGCGTCGCTTGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                                                <  2:1174549/150‑1 (MQ=255)
                                        gCCCGAGCCGGTTGCCTGATGCTACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCTGATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                                               <  1:1773723/150‑1 (MQ=9)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   <  1:847935/130‑1 (MQ=31)
                                        gCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCt                                                                                                                                   >  2:847935/1‑130 (MQ=31)
                                                                                                           aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCg                                            <  1:4313541/150‑1 (MQ=11)
                                                                                                             cccGTAGGTCTGATAAGGCGGTAACGCCGCATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                          <  1:2501168/150‑1 (MQ=255)
                                                                                                             cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                          >  1:1894830/1‑150 (MQ=17)
                                                                                                             cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGAc                                          >  2:1216879/1‑150 (MQ=32)
                                                                                                                gTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATc                                       >  1:3966157/1‑150 (MQ=32)
                                                                                                                 tAGGTCTGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATcc                                      <  1:4241725/150‑1 (MQ=17)
                                                                                                                   ggTCGGATACGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCaa                                    <  1:4733420/150‑1 (MQ=21)
                                                                                                                    gTCGGATAAGGGGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAAc                                   <  1:3732706/150‑1 (MQ=21)
                                                                                                                     tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGGGTAGTGCACAACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCGACATCAAACg                                  >  2:5773702/1‑150 (MQ=11)
                                                                                                                      cGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGt                                 <  2:161223/150‑1 (MQ=35)
                                                                                                                       ggATAAGGCGGTCACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTc                                <  2:2102299/150‑1 (MQ=25)
                                                                                                                        gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                               >  2:4176402/1‑150 (MQ=37)
                                                                                                                        gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTcc                               >  1:4973239/1‑150 (MQ=37)
                                                                                                                        gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGAATCCGACATCCAACGTcc                               <  1:2110118/150‑1 (MQ=255)
                                                                                                                         aTAAGGGGGTGACGCCGGTTCCGTCATCTAACGCCCTTGCCGGTTGCCTGATGCGACTCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCg                              <  1:4028439/150‑1 (MQ=11)
                                                                                                                         aTAAGGCGGTTACGCCGCATCCGACATCTAACGACCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGACTACACAGCAGGGAACTGCTCCACACCGTAGCAAGTATAAGGCGGTTAATCCGCATCAGACATCCAAAGTCCg                              >  1:1077796/1‑150 (MQ=11)
                                                                                                                          tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCGGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  1:3035093/150‑1 (MQ=255)
                                                                                                                          tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  2:391345/150‑1 (MQ=37)
                                                                                                                          tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  2:2427017/150‑1 (MQ=37)
                                                                                                                          tAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGATCCACCCCGTAGGTTGGATATGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  1:637454/150‑1 (MQ=255)
                                                                                                                          gacggCGGTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGa                             <  1:3035094/147‑1 (MQ=255)
                                                                                                                             ggCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGcc                          <  1:5339039/150‑1 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         <  2:1644155/150‑1 (MQ=37)
                                                                                                                              gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCg                         >  2:4435236/1‑150 (MQ=37)
                                                                                                                               gtgTTACGCCGGTTGCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCGGGCGCTTATTATCTGGCATACACCGCTGTGAAGTGCTCCACCCGGTTGGTTGTTTAAGGCGGTTACGCCGCATCCTACATCCAACGTCCGAGCCgg                        <  1:550047/148‑1 (MQ=11)
                                                                                                                               cGGTTACGCCGAATCCGACATCTAACGCACTTTCCGGTTTCCTGTTGCGACGGTTGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCgg                        <  2:381540/150‑1 (MQ=11)
                                                                                                                                 gTTACGCCGCATCCGACATCTAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                      <  1:3867086/150‑1 (MQ=37)
                                                                                                                                 gTTACGACGCATCCTACAGCTACCGCGCTTGCCGGTTGCCTTATGCTAATCTGTCGCGTCTTATGTGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGtt                      <  1:2772932/150‑1 (MQ=11)
                                                                                                                                  acaCGCCGCCCCCGACATCCCACGCCCTTTTCGTTTGCCTGTTGCGAAGCTGGATCGTCTTATCATGACTACAACGATGTGAAGTGCTCGACCGCGTTTTTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTg                     <  2:950330/148‑1 (MQ=2)
                                                                                                                                   caCGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGATGTGCTCCACCCCGTAGTTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGc                    <  1:5102266/149‑1 (MQ=11)
                                                                                                                                     cgccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCAGCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  2:3059649/150‑1 (MQ=37)
                                                                                                                                     cgccgcATCCGACATCCAACGCCCTAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  1:3480838/150‑1 (MQ=255)
                                                                                                                                     cgccgcATCAGACATCTAACGCACAAGACTTTTGCCTGATGCGAAGCTGTAGAGTCTTAACAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTTGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCt                  <  1:745247/150‑1 (MQ=11)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                >  1:682586/1‑150 (MQ=37)
                                                                                                                                       ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGATACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGa                >  2:3118588/1‑150 (MQ=37)
                                                                                                                                        cgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGAt               <  1:5454159/150‑1 (MQ=37)
                                                                                                                                           aTCCGACATCTAACGCCCAAGCCGGTAGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCACTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCAACAGACCTACAACGTCCGAGCCGGAAGCCTGCTGCg            >  2:2976858/1‑150 (MQ=11)
                                                                                                                                            tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTCCACCGCTGGGACGTGCACCGCCCCGTAGGTTGGATCAGGCGGTTGCGCCGCAGCCGACATCGATGGGCCGTGGCGGTTGCCTGAGGCgc           >  1:5621530/1‑149 (MQ=11)
                                                                                                                                              cGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACg         >  1:3544794/1‑150 (MQ=17)
                                                                                                                                               gACATCTAACGCCCAAGCCGGTTGCCTGTTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATgcggcgt        <  2:1463248/150‑2 (MQ=255)
                                                                                                                                                  aTCTAACGCCCCCGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCGCCACCGCTGGGACGTGCGCCACCCCGGAGGTTGGGGCAGCCGGTTACGCCGCATCCGACATCGAACGGCCGAGCCGGGTGCCTGAGGCGACGCggg     >  2:4385039/1‑150 (MQ=11)
                                                                                                                                                  aTCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTgg     <  2:2438555/150‑1 (MQ=21)
                                                                                                                                                    cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcg   >  2:2700714/1‑150 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCCTCCGACATCCAACGTCCGAGCCGGTTGCCTGCTGCGACGCTGgcgc  <  2:5766337/150‑1 (MQ=255)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGgcgc  <  2:5301241/150‑1 (MQ=37)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGACCGAGCCGGTTGCCTGATGCGACGCTGgcgc  >  1:5759315/1‑150 (MQ=21)
                                                                                                                                                     tAACGCCCAAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCGCCCCGGAGGTTGGAGCAGGCGGGTACGCCGCAGCCGACCGCGACCGGCCGGGCCGGTGGCCTGCGGCGCCGCGGgcgg  >  2:5699099/1‑149 (MQ=2)
                                                                                                                                                     |                                                                                                                                                     
TAGGTCAGACAAGGCGGTCACGCCGCATCCGACATCCAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCCAACGTCCGAGCCGGTTGCCTGATGCGACGCTGGCGC  >  NC_000913/2304531‑2304829

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: