Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,304,988 T→C 5.4% intergenic (+595/+120) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,9880TC 5.4% 871.1 / 24.1 298intergenic (+595/+120)eco/mqoecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
Reads supporting (aligned to +/- strand):  ref base T (160/113);  new base C (9/7);  total (175/123)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

ACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTG  >  NC_000913/2304864‑2305137
                                                                                                                            |                                                                                                                                                     
aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCa                                                                                                                              >  2:2215602/1‑150 (MQ=11)
aCCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCATACACAGCTGAGAAGTGCACCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCa                                                                                                                              >  2:2538545/1‑150 (MQ=2)
 ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCaa                                                                                                                             >  2:4118431/1‑150 (MQ=31)
 ccccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCaa                                                                                                                             >  2:4303555/1‑150 (MQ=31)
   ccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGAATAGACAGATGTGTAGTTCTACTCCCCGTAGGTTGGATAAGTAGGTTACGCAGAATCCAAct                                                                                                                           >  1:4315029/1‑149 (MQ=11)
    cGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACAt                                                                                                                          <  2:177173/150‑1 (MQ=32)
        ggTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACACCGCATCCAACATCTaa                                                                                                                      <  1:4707908/150‑1 (MQ=21)
         gTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAAc                                                                                                                     >  1:1422742/1‑150 (MQ=34)
          tCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACg                                                                                                                    >  2:1616433/1‑150 (MQ=34)
            ggATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGcc                                                                                                                  >  2:1309333/1‑150 (MQ=35)
             gATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGccc                                                                                                                 >  2:1131384/1‑150 (MQ=37)
                  ggCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCGAGcc                                                                                                            >  2:1350655/1‑150 (MQ=21)
                   gCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCg                                                                                                           >  2:3232216/1‑150 (MQ=37)
                     ggTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGAGGCTGGCGCTTCTTATCAGGCCTACACCGCTGTGACGTGCTCCCCCCCGTTGGTGGGATATGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGt                                                                                                         <  1:1350454/150‑1 (MQ=11)
                       ttACTCCTCATCCGAAATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTCAAGTGCTCCACCCCGTAGTTTGTATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTg                                                                                                       <  1:5667551/150‑1 (MQ=11)
                          cgccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTTGGTTGGATACGGCGGTTACGCCGCAACCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                    <  1:1151884/150‑1 (MQ=255)
                            ccgcATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGa                                                                                                  >  1:3072735/1‑150 (MQ=37)
                              gcACCCGACATCTAACGCCCAAGCCGGTTGCGTGCTGCGACGGTGGCGCGTCTTATCAGGCCTACACCGCTGTGACGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATg                                                                                                <  1:50562/150‑1 (MQ=11)
                                aTCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGTCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATgag                                                                                              >  2:5568881/1‑148 (MQ=37)
                                 tCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGa                                                                                             <  1:3282220/150‑1 (MQ=37)
                                  ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                            >  2:3953271/1‑150 (MQ=37)
                                  ccGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCAAACATCTAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                            <  1:2252704/150‑1 (MQ=37)
                                  ccGACATCAAACGCCCCCGCCGGTTGCCTGATGCGACGCCGGCGCGTCTTATGAGGCCTCCCCCGCAGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGAc                                                                                            <  2:1943549/150‑1 (MQ=11)
                                    gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGc                                                                                          >  1:1234796/1‑150 (MQ=37)
                                    gACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAGCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGGTGGATAAGGCGGTTACGCCGCATCCACCAGCTAACGCCCGAGCCGGTTGCCTGAGGCGACGc                                                                                          >  2:3373688/1‑150 (MQ=11)
                                        tctcccGCCCCCGCCGGTTGCCGGAGGCGACGCGGGCGCGTCTTATCCGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                      <  1:2166240/145‑1 (MQ=11)
                                        tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                      >  1:4242443/1‑150 (MQ=37)
                                        tCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTACGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                      >  1:3443689/1‑150 (MQ=37)
                                         cTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGGTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcg                                                                                     >  1:2811893/1‑150 (MQ=37)
                                          tAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgcgc                                                                                    >  1:1068139/1‑150 (MQ=37)
                                            aCGCCCAAGCCGGTTGCCCGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                    >  2:277127/1‑132 (MQ=37)
                                              gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                <  1:2611046/150‑1 (MQ=37)
                                              gCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTATTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCt                                                                                <  1:1018135/150‑1 (MQ=37)
                                                ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATCAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGAGGCGACGCTGGCGCGTCTTa                                                                              >  2:5538095/1‑150 (MQ=255)
                                                ccAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCCCTGTGAAGTGCACCACCCCGAAGGTTGGATAATGCGGTTACCCCGCATCCAACATCTAACGCCCCAGCCGGTTGCCAGAATCGACGCTGGCGCGTCTTa                                                                              >  1:2553383/1‑150 (MQ=11)
                                                 cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCACCACCCCCTAGGTTGGATAAGGCGGTTACGCCGCATCCAACAACTAACGCCCGAGCCGGGTGCCAGATGCGACGCTGGCGCGGCTTAt                                                                             >  1:2402412/1‑150 (MQ=11)
                                                 cAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGGTGCCAGATGCGACGCTGGCGCGGCTTAt                                                                             >  1:2005185/1‑150 (MQ=255)
                                                  aaGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATc                                                                            <  1:3232216/150‑1 (MQ=37)
                                                   aGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCa                                                                           >  2:4756150/1‑150 (MQ=37)
                                                        gTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATa                                                                      >  2:2201466/1‑150 (MQ=40)
                                                           gCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                   <  2:4062368/150‑1 (MQ=255)
                                                           gCCTGATGCGACGCTGGCGCGTCTTATCAGGCCAACACCGCTGTGAAGGGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTAACAGCATACGc                                                                   <  1:2440602/150‑1 (MQ=255)
                                                            ccTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGcc                                                                  <  1:2084940/150‑1 (MQ=255)
                                                             cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCca                                                                 >  1:734065/1‑150 (MQ=255)
                                                             cTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCca                                                                 >  1:881644/1‑150 (MQ=255)
                                                              tGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCcac                                                                >  2:3602303/1‑150 (MQ=255)
                                                                aTGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCt                                                                                                                        <  2:2007129/92‑1 (MQ=34)
                                                                 tGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                    <  1:96840/111‑1 (MQ=39)
                                                                   cGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCCACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCACACCACACATCCg                                                           >  2:3816270/1‑150 (MQ=11)
                                                                    gACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCgg                                                          >  2:652810/1‑150 (MQ=255)
                                                                    gACGCGGGCGCGTCTTCTCAGGCCCACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTTCGGCGCATCCATCATCTAAGGGCCCAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCgg                                                          <  1:2029857/150‑1 (MQ=14)
                                                                     aCGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCAAACGACCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCAAAACCCACCTCAGGc                                                         >  1:5351495/1‑150 (MQ=11)
                                                                       gCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTAGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGcc                                                                                                     >  1:1718873/1‑104 (MQ=25)
                                                                          ggCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGc                                                                         <  1:4099362/129‑1 (MQ=39)
                                                                          ggCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATAcc                                                    >  2:2819238/1‑150 (MQ=255)
                                                                             gcgTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGgc                                                                                      <  1:3549186/113‑1 (MQ=39)
                                                                               gTCTTATCAGGCCTTCACCGCTGTGAAGTGCTCCACCCCTTAGGTTTCTTAAGGCGTTTAGGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGcc                                               <  1:4313894/150‑1 (MQ=18)
                                                                               gTCTTATCAGCCCTACACCGCGGTGAAGTGCTCCACCCCGTAGGTTCGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTGATCAGCATACGCCACATCCGGCATCCCCTGcc                                               >  2:205988/1‑150 (MQ=17)
                                                                                tCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCg                                              <  1:4945395/150‑1 (MQ=255)
                                                                                  ttATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGa                                            <  2:5432211/150‑1 (MQ=255)
                                                                                  aaaTCAGGCCTTCACCGCTGTGACGAGCTCCACCCCTTAGGTTGGATAAGGCGGTCACGCCGCATCCTACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGa                                            <  2:1695313/148‑1 (MQ=18)
                                                                                   tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGtt                                                                                                        <  2:5066250/89‑1 (MQ=255)
                                                                                   tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGtt                                                                                                        >  1:3800475/1‑89 (MQ=255)
                                                                                   tATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAt                                           <  1:3118588/150‑1 (MQ=255)
                                                                                     tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGAtgt                                         <  1:4815034/150‑1 (MQ=255)
                                                                                     tCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCCTGCCGGAtgt                                         >  1:4540438/1‑150 (MQ=255)
                                                                                       aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCGACATCGAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATGGTATGCCGGATGTgg                                       <  2:3544794/150‑1 (MQ=17)
                                                                                       aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCTTACCATGCCGGATGTgg                                       >  2:1441941/1‑150 (MQ=255)
                                                                                       aGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTgg                                       >  2:586708/1‑150 (MQ=255)
                                                                                        ggCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                    >  1:1504035/1‑88 (MQ=255)
                                                                                        ggCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCt                                                                                                    <  2:1504035/88‑1 (MQ=255)
                                                                                          ccTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCGTCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGt                                    >  2:4662156/1‑150 (MQ=255)
                                                                                          ccTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGt                                    >  1:2890335/1‑150 (MQ=255)
                                                                                             acacCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATc                                 <  2:728327/150‑1 (MQ=255)
                                                                                               acCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGt                                                                                  <  2:2710733/99‑1 (MQ=40)
                                                                                               acCGCTGTGAAGTGCTCCACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTc                                                                                 <  2:2959873/100‑1 (MQ=18)
                                                                                                ccGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCAAACGCCCGAGCCGGTTGcc                                                                                                     >  2:2356364/1‑79 (MQ=255)
                                                                                                 cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGa                                            >  2:2374208/1‑135 (MQ=255)
                                                                                                 cGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGa                                            <  1:2374208/135‑1 (MQ=255)
                                                                                                 cGCCGTGAAGGGCGCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCAtaa                             <  1:5773702/150‑3 (MQ=255)
                                                                                                    tgtgAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACaa                          <  1:4009388/150‑1 (MQ=255)
                                                                                                        aaGTGCTCCACCCCGTAGGTTGGATACGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGTTGTGGCGTATCATTACAACGCa                      <  1:119691/150‑1 (MQ=255)
                                                                                                             cTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATc                 >  1:3344463/1‑150 (MQ=255)
                                                                                                              tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCTACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATcc                <  1:4357751/150‑1 (MQ=255)
                                                                                                              tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTCTCATTACAACGCAATATcc                >  1:2478145/1‑150 (MQ=255)
                                                                                                              tCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCAAGCCGGATGTGGCGTATCATTACAACGCAATATcc                >  2:4397398/1‑150 (MQ=255)
                                                                                                                cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc              <  1:685796/150‑1 (MQ=255)
                                                                                                                cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc              <  1:3602303/150‑1 (MQ=255)
                                                                                                                cACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTGATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGc              >  1:3159823/1‑150 (MQ=255)
                                                                                                                 aCCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGcc             >  1:5540852/1‑150 (MQ=255)
                                                                                                                  ccccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCa            >  2:5737570/1‑150 (MQ=255)
                                                                                                                  ccccGTAGGACGGATAAGGCGGATACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCa            <  2:3750803/150‑1 (MQ=255)
                                                                                                                   cccGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGAtg                                          >  2:1216912/1‑119 (MQ=255)
                                                                                                                    ccGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATCTCCGCCAct          >  2:916741/1‑150 (MQ=255)
                                                                                                                      gTAGGTCGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGc                                                                   <  1:1801752/91‑1 (MQ=255)
                                                                                                                          gTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt    <  1:1311733/150‑1 (MQ=255)
                                                                                                                          gTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt    >  2:1846940/1‑150 (MQ=255)
                                                                                                                          gTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCtttt    >  2:1282056/1‑150 (MQ=255)
                                                                                                                           ttGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCttttt   >  2:3437721/1‑150 (MQ=255)
                                                                                                                            tGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTg  >  2:5008107/1‑150 (MQ=255)
                                                                                                                            |                                                                                                                                                     
ACCCCGTAGGTCGGATAAGGCGGTTACGCCGCATCCGACATCTAACGCCCAAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACACCGCTGTGAAGTGCTCCACCCCGTAGGTTGGATAAGGCGGTTACGCCGCATCCAACATCTAACGCCCGAGCCGGTTGCCTGATGCGACGCTGGCGCGTCTTATCAGCATACGCCACATCCGGCATACCATGCCGGATGTGGCGTATCATTACAACGCAATATCCGCCACTTCTTTTTG  >  NC_000913/2304864‑2305137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: