Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,470,439 G→T 8.0% D437Y (GAC→TAC) ‡ gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4390GT 8.0% 1109.0 / 3.2 371D437Y (GAC→TAC) ‡gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base G (136/187);  new base T (27/3);  total (180/191)
Fisher's exact test for biased strand distribution p-value = 2.97e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 376 total aligned reads displayed.

TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588
                                                                                                                                                     |                                                                                                                                                     
 ttttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                                                                                                                      <  1:4479932/150‑1 (MQ=255)
  tttGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGaa                                                                                                                                                     >  2:436794/1‑149 (MQ=255)
      gAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATAAAGAGATTTAAGCAAGCATTTATTTGGAATATATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATCAAAGAAATAAAGACaaaa                                                                                                                                                 >  2:872975/1‑148 (MQ=255)
       aaTATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAAAATAATTAAAAGAAAACAAGAAAATAg                                                                                                                                                >  2:5473358/1‑150 (MQ=255)
          atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                             <  2:2045534/150‑1 (MQ=255)
          atCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                                                                                                             <  2:2973710/150‑1 (MQ=255)
            ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAATATAGATg                                                                                                                                           >  1:4857957/1‑150 (MQ=255)
            ctcAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATAAATACAAAAGCGaag                                                                                                                                           >  1:2711395/1‑148 (MQ=255)
             tcAATTTTATGTGTCATTTTACGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAAAAAATAAAAGAAAACAATACAAAATCCATGt                                                                                                                                          >  2:3646143/1‑150 (MQ=255)
               aaTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAATAAATAAAGAAAAAAGCGATGTaa                                                                                                                                        >  1:2357788/1‑150 (MQ=255)
                aTTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTaaa                                                                                                                                       <  2:4315303/150‑1 (MQ=255)
                  tttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                                                                                                                     <  1:3904870/150‑1 (MQ=255)
                    tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAAAAAAGAAAATATCGAAGTAAATCAc                                                                                                                                   >  1:1497340/1‑150 (MQ=255)
                        tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                                                                                                               <  1:4641279/150‑1 (MQ=255)
                         gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATCAAATAAAAAAAGAAAAAAGAGAAGGTAATCACAAAGt                                                                                                                              >  1:4573609/1‑150 (MQ=255)
                          tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                                                                                                             >  2:1899888/1‑150 (MQ=255)
                             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                          <  1:265116/150‑1 (MQ=255)
                             tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                                                                                                          <  1:265304/150‑1 (MQ=255)
                               tttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAAGAAATAAACACAAAAACGATGTAAAGCACTAAGGTTAAAt                                                                                                                        >  1:166576/1‑150 (MQ=255)
                                ttCGGGGCAGGATAGAGAGCTATAAGCAACCATTTATTTGGAATGTATTTGTCGTTCGACCTTTGCTTTTACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAAtt                                                                                                                       <  1:5791882/150‑1 (MQ=255)
                                ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGTAATGAAGACAATAGCGTTTTAAAGGAATAATTTTAAAtt                                                                                                                       >  2:4051900/1‑150 (MQ=255)
                                ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTAAAATAAATAAAGAAAAAAGCGGTGTTAATCAAAAAGTTTTAAtt                                                                                                                       >  1:1000067/1‑150 (MQ=255)
                                   ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                                    <  1:1384729/150‑1 (MQ=255)
                                   ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                                    <  1:4313795/150‑1 (MQ=255)
                                   ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                                                                                                                    <  1:4022682/150‑1 (MQ=255)
                                        aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCa                                                                                                               <  1:297825/150‑1 (MQ=255)
                                         ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAATTAAAAGAAATAAATACAATATCGATGTAAAGCACTAAGTTTAAATTGCGCGCCaa                                                                                                              >  1:3253590/1‑150 (MQ=255)
                                           atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAATTTAAAGAAATCAAGTCAATATCGATGTTAAGGACTAAGTTTTAATTTGGCGCCAATc                                                                                                            >  2:5130827/1‑150 (MQ=255)
                                           atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATAAAAATAAATCAAAAAAAAAGAGATTTTAATAACAAAGTTTAAATATAGCGCCaaaa                                                                                                            >  1:4688702/1‑148 (MQ=255)
                                              taGAGATTTAAGCAAGCATTTATTTTGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATg                                                                                                         <  1:3678363/150‑1 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTTTAAAATAAATAAAAAAAAAAAAAAAAAAAGCGATGTAAATAAAAAAATTTAAATTTAGCGCCaaaaaagg                                                                                                        >  1:1794807/1‑144 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAAGTAAAGCACTAAGTTTAAATTGCGCGCCAATCAAgg                                                                                                        >  2:5723635/1‑150 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAAGACAAAAGCGATTTAAATCACAAAGTTTAAATTTCGCGCCAAGCATgg                                                                                                        >  2:2737256/1‑150 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAAATAAAAGAAATAAAGACAAAAGCGATGTAAAGAACAAAGTTTAAATTTCGAGCCaaaaaagg                                                                                                        >  2:4892898/1‑144 (MQ=255)
                                               agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTGTTTTTTATTTATTAAATAATTAAAATAAATCAATAAAAAAACGATTTAAAGGAAGAAGTTTAAATTTAGCGCCaaaaaatg                                                                                                        >  1:57573/1‑144 (MQ=255)
                                                gagaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAAAAAAAAAAAACAAAACACATGTAAATCAATAAGTTTAAAATTAGCGCCCAACAAGgc                                                                                                       >  1:1738917/1‑150 (MQ=255)
                                                 agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTAATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAAAATATCGATGTAAAGAACTAACTTTAAATTTCGCGCCAATCATTgcg                                                                                                      >  2:5273260/1‑150 (MQ=255)
                                                     ttAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTAAAAGAAATAAAGAAAAAAGCGAAGTAAAACACAAAGTTTAAATTTCGCGCCAAAAAAGGCGCGca                                                                                                  >  2:3127959/1‑150 (MQ=255)
                                                         gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAAGACAATAGCGATGTAAATCACTAAGTTTAAATTTCGCGCCAATAATGGCGCGCACAAg                                                                                              >  2:907757/1‑150 (MQ=255)
                                                          caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc                                                                                             <  2:1662073/150‑1 (MQ=255)
                                                                 ttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTataata                                                                                      <  2:1162874/150‑1 (MQ=255)
                                                                      tgtaaTGTATTTTTGGTTCTACCTTTTTTTATAATTAACTTTTTTTTTTTTTATTTTTAAAATTATAAAAATAAATCAATACTTTAGCTATTTAATTCACTAATTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCTATAATACCAAc                                                                                 <  1:1836116/147‑1 (MQ=255)
                                                                           tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAAATAAAAGAAAACAAAAAAAAATAGATTTAAATCACTAAGTTTAAATTTAGCGCCAATAAAGTCGCGCACAAGCAATAATAACaaacaaaa                                                                            >  2:5427539/1‑144 (MQ=255)
                                                                           tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAAATTATAAAATAATTCAAAGAAAACAATACAAAAGCGAAGTAAACCACAAAGTTTAAAATTCGCGCCAAACATGGCCCGCACAACCTATAAAAACAACCAAAt                                                                            >  2:569909/1‑150 (MQ=255)
                                                                             tATTTTTCTTTCTACTTTTGATTATACATAACTTTTTTTTTTTTAATTTATAAAATAATTAAAATAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAAttt                                                                          <  1:240736/150‑1 (MQ=255)
                                                                               tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTTATTTATAATATAAATAAAAGAAAAAAAAACAAAAGAGATGTAAATAAATAATTTTAAATTTGGGGGCCATAATGGCGCGCACAAGCGAAAATAAAAAACAAAtttgt                                                                        >  1:3501988/1‑148 (MQ=255)
                                                                               tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATACAAAGAAATAAAGACAAAAGCGATGTAAAGCACAAAGTTTAAATTGCGCGCCCATCATGGCGCGCACCAGCTATAATAACAACCAAATTtct                                                                        >  2:4549156/1‑150 (MQ=255)
                                                                                 tttCGTTCGACCTTTGCTTATAAATAACAGTCTTTTTTTTAATTTATAAAATATTTCAAATAAATCAATACAATAGCTATGTAAAGCACTAAGTTTAATTTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcc                                                                      <  1:1899888/150‑1 (MQ=255)
                                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTctcctcct                                                                  <  2:1716586/150‑1 (MQ=255)
                                                                                     gttcgACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAAAAAATAAAATAAATAAAGAAAAAATCGATTTAAAGCAAAAAGTTTAAAATTAGCGCCAATAATGGCGCGCACAAGCTATAATAACAAACTAATATatactcca                                                                  >  2:4178689/1‑149 (MQ=255)
                                                                                       tcgACCTTTTCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCctct                                                                <  2:3057949/150‑1 (MQ=255)
                                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGATATCAAGACAATAGCGATGTAAAGCACTATGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTa                                                              <  2:2822559/150‑1 (MQ=255)
                                                                                         gACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATAAACACAATAGCGATGTAAAGCACAAAGTTTAAATTGCGCCCCAATAATGGCGCGCACAAGCTATAATAACAAACAAATTTATCCACCGCTTa                                                              >  2:1340647/1‑150 (MQ=255)
                                                                                           ccTTTGATTATACTTATTTGTTGTTTTTTTAATTTATATAATAATTAAAAGAAATCAAGACAATAGCGATGTAACGCACTAATTTTAATGTGCGCGCCAATCTTGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                            <  1:567825/150‑1 (MQ=255)
                                                                                           catttGCTTATACATAAAATACGTTTTTTTAATTTAAAAAATAATAAAAAAAAATCAAGACAATAGCCATGAAAACCACAAAGTTTAAATTGCCCCCCAATAATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTaga                                                            <  1:667666/148‑1 (MQ=255)
                                                                                             tttGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGt                                                          <  2:1853875/150‑1 (MQ=255)
                                                                                               tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTATGTTTAAATTGCGCGCCACTCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  1:2462122/150‑1 (MQ=255)
                                                                                               tGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGa                                                        <  2:2939412/150‑1 (MQ=255)
                                                                                                        aTAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATAtctc                                               <  2:3330232/150‑1 (MQ=255)
                                                                                                         tAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTcc                                              <  1:2341811/150‑1 (MQ=255)
                                                                                                          aaCAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCt                                             <  1:2227839/150‑1 (MQ=255)
                                                                                                               tCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAg                                        >  2:2232881/1‑150 (MQ=255)
                                                                                                                cGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                       <  1:1608419/150‑1 (MQ=255)
                                                                                                                cGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCAAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGa                                       <  1:4395139/150‑1 (MQ=255)
                                                                                                                  tttttttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAAt                                     >  1:3700188/1‑150 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 <  2:625820/150‑1 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 <  1:4223042/150‑1 (MQ=255)
                                                                                                                      tttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCg                                 >  2:2367538/1‑150 (MQ=255)
                                                                                                                       ttAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCgg                                >  2:2496416/1‑150 (MQ=255)
                                                                                                                        tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                               <  2:761110/150‑1 (MQ=255)
                                                                                                                        tAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGt                               >  1:1952289/1‑150 (MQ=255)
                                                                                                                         aaTTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTa                              >  2:3811331/1‑150 (MQ=255)
                                                                                                                             tataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGa                          <  2:2998649/150‑1 (MQ=255)
                                                                                                                              ataAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGAc                         <  2:3943673/150‑1 (MQ=255)
                                                                                                                               taAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACt                        >  2:1559902/1‑150 (MQ=255)
                                                                                                                                aaaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTCTTGACTa                       >  1:5539234/1‑150 (MQ=255)
                                                                                                                                 aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                      >  2:690477/1‑150 (MQ=255)
                                                                                                                                 aaaTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTAt                      >  1:4270659/1‑150 (MQ=255)
                                                                                                                                  aataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGATTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCACTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATc                     <  1:5228542/150‑1 (MQ=255)
                                                                                                                                   ataatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAAAACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCa                    >  2:4832099/1‑150 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  2:4934922/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  1:3191717/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  2:4697231/150‑1 (MQ=255)
                                                                                                                                     aatTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCaaa                  <  1:3239030/150‑1 (MQ=255)
                                                                                                                                       ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                >  1:113869/1‑150 (MQ=255)
                                                                                                                                       ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                <  1:3135806/150‑1 (MQ=255)
                                                                                                                                       ttCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATg                >  2:106021/1‑150 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  1:5285716/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  1:5087183/150‑1 (MQ=255)
                                                                                                                                         cAAAGAAATCAAGACAATACCGATGTAAAGCACTAAGATTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGcc              <  2:4119053/150‑1 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             >  2:3611735/1‑150 (MQ=255)
                                                                                                                                          aaaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGccc             <  1:1226175/150‑1 (MQ=255)
                                                                                                                                           aaGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCt            >  1:2895889/1‑150 (MQ=255)
                                                                                                                                              aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt         <  1:73725/150‑1 (MQ=255)
                                                                                                                                              aaaTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGAt         <  1:1293209/150‑1 (MQ=255)
                                                                                                                                                aTCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTc       <  1:4978861/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  1:5466440/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    <  1:5053598/150‑1 (MQ=255)
                                                                                                                                                   aaGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgtt    >  1:4835173/1‑150 (MQ=255)
                                                                                                                                                     gACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCgttgt  <  2:2674950/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTTTTGGAATATCTCAATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTATAATACCAACCTAATTTCTCCTCCTCTTAGAGTGACTATATCTCCTGATAGAATTGCGGTATTGACTATCAAATGCCCTGATTCGTTGT  >  NC_000913/2470290‑2470588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: