Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,637,438 T→G 5.3% intergenic (‑82/+36) der ← / ← bamB GTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,637,4380TG 5.3% 1125.2 / 36.4 313intergenic (‑82/+36)der/bamBGTPase; multicopy suppressor of ftsJ/BamABCDE complex OM biogenesis lipoprotein
Reads supporting (aligned to +/- strand):  ref base T (140/156);  new base G (11/6);  total (151/162)
Fisher's exact test for biased strand distribution p-value = 2.13e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 314 total aligned reads displayed.

GTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAA  >  NC_000913/2637289‑2637583
                                                                                                                                                     |                                                                                                                                                 
      cGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGa                                                                                                                                             <  2:4996345/150‑1 (MQ=255)
         ttAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGcc                                                                                                                                          >  1:3701717/1‑150 (MQ=255)
          tAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCg                                                                                                                                         >  2:5789870/1‑150 (MQ=255)
             aTAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGttt                                                                                                                                      >  1:1523279/1‑150 (MQ=255)
               aaagTGGATTTTCCTACGTTAGGGCGCCCGACACGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGCCAACGCACTCTATTTTCGCGTCGTTGTTAAAAACAGGAAAACGGCTCCTGGACAGGGGCCGTTTTc                                                                                                                                    <  2:5045680/147‑1 (MQ=255)
                  gTGGATTTTCCTACGTTAGGGCGCCCGACACGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                                 <  2:4635145/150‑1 (MQ=255)
                  gTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                                 >  1:3839048/1‑150 (MQ=255)
                  gTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCaaa                                                                                                                                 <  2:1696725/150‑1 (MQ=255)
                     gATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTg                                                                                                                              >  1:1708126/1‑150 (MQ=255)
                      aTTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGa                                                                                                                             <  2:4567475/150‑1 (MQ=255)
                       ttttCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGaa                                                                                                                            <  2:2897554/150‑1 (MQ=255)
                        tttCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCCTGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAAc                                                                                                                           <  2:3998854/150‑1 (MQ=255)
                         ttCCTACGTTAGGGCGCCCGACAAGCGCGACCCCAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACg                                                                                                                          >  2:3158695/1‑150 (MQ=255)
                           ccTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGAc                                                                                                                        >  2:2811853/1‑150 (MQ=255)
                             tACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACag                                                                                                                      >  1:3362280/1‑150 (MQ=255)
                              aCGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACaga                                                                                                                     <  2:2060477/150‑1 (MQ=255)
                                gTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAgaga                                                                                                                   >  1:2555442/1‑150 (MQ=255)
                                 ttAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAc                                                                                                                  >  2:1531952/1‑150 (MQ=255)
                                 ttAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGAc                                                                                                                  <  2:1676899/150‑1 (MQ=255)
                                   aGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGa                                                                                                                <  2:2010378/150‑1 (MQ=255)
                                     ggCGCCCGACAAGCGCGACCCCAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGagt                                                                                                              >  2:629774/1‑148 (MQ=255)
                                        gcCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                                                                                           <  2:900414/150‑1 (MQ=255)
                                        gcCCGACAAGCGCGAACACAGGTACCATGTTTAAAGCCTCTTTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTTTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAc                                                                                                           <  2:3912841/150‑1 (MQ=255)
                                         cccGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACg                                                                                                          >  2:4274058/1‑150 (MQ=255)
                                          ccGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCGGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgt                                                                                                         >  2:5387054/1‑150 (MQ=255)
                                            caCAAGCCCCACCACAGCAACCATCTTAAAAGCCACATTTTAATAAATCATCACACAACCCACCCAATATTCGCGTCGTTGATAAAAACACCAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACgtgt                                                                                                       <  2:2562868/149‑1 (MQ=255)
                                             aCAAGCGCGACCTCAGGTACCATGTTTAAAGCCTCATTTTTATAACTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTa                                                                                                      <  2:1317674/150‑1 (MQ=255)
                                                 gcgcGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACCACGCACGCTATATTCGCGTCGTTGTTAAACACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATag                                                                                                  <  2:2655149/150‑1 (MQ=255)
                                                 gcgcGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAAGTGTAATag                                                                                                  >  1:3471531/1‑150 (MQ=255)
                                                  cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                                 >  1:4187718/1‑150 (MQ=255)
                                                  cgcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATaga                                                                                                 <  2:4795126/150‑1 (MQ=255)
                                                   gcgACCACAGGTACCCTGTTAAAAGCCTCATTTTTATAACTCATCAGCCAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATagag                                                                                                <  2:5117018/150‑1 (MQ=255)
                                                   gcgACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACTCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAAAGACAGAGACTATTAACCTGTAATagag                                                                                                >  1:1002645/1‑150 (MQ=255)
                                                        cacaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                           >  1:3875692/1‑150 (MQ=255)
                                                        cacaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTacac                                                                                           >  1:2988520/1‑150 (MQ=255)
                                                         acaGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACg                                                                                          >  1:2678593/1‑150 (MQ=255)
                                                            ggTACAATGTTTAAACACTCATTTTTAAAAATCAACACACACCGCACCCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGtt                                                                                       <  1:1380261/150‑1 (MQ=255)
                                                             gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTc                                                                                      >  1:4070076/1‑150 (MQ=255)
                                                             gTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTc                                                                                      >  1:4317698/1‑150 (MQ=255)
                                                                ccATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGt                                                                                   <  2:3412645/150‑1 (MQ=255)
                                                                 cATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTc                                                                                  <  2:3230659/150‑1 (MQ=255)
                                                                       tAACGCCTCATTTTTATAAATCATCAGACACCGCACGCTATATTCCCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGc                                                                            <  2:4395271/150‑1 (MQ=255)
                                                                        aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGATCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTAAACGGTTCCGTATTTTGcc                                                                           >  2:1872254/1‑150 (MQ=255)
                                                                        aaaGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGcc                                                                           >  1:4662439/1‑150 (MQ=255)
                                                                          aGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTg                                                                         <  1:3997700/150‑1 (MQ=255)
                                                                               cATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATca                                                                    >  2:2498212/1‑150 (MQ=255)
                                                                               cATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATca                                                                    >  2:2216381/1‑150 (MQ=255)
                                                                                 tttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcagc                                                                  >  1:4088494/1‑150 (MQ=255)
                                                                                  ttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcagca                                                                 <  1:917760/150‑1 (MQ=255)
                                                                                  ttttATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATcagca                                                                 >  2:4439323/1‑150 (MQ=255)
                                                                                         aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                          <  2:4521659/150‑1 (MQ=255)
                                                                                         aaTCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGcc                                                          >  1:4858863/1‑150 (MQ=255)
                                                                                            catcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTc                                                       <  1:1237247/150‑1 (MQ=255)
                                                                                             atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCa                                                      <  2:3362280/150‑1 (MQ=255)
                                                                                             atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCa                                                      <  2:4930194/150‑1 (MQ=255)
                                                                                             atcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCGTTTGCCTGGATCAGCAGTTTGCCGTCa                                                      >  2:968653/1‑150 (MQ=255)
                                                                                              tcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAg                                                     >  1:5008496/1‑150 (MQ=255)
                                                                                              tcaGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAg                                                     >  1:3531469/1‑150 (MQ=255)
                                                                                                aGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCg                                                   >  1:715075/1‑150 (MQ=255)
                                                                                                aGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCg                                                   >  2:3154512/1‑150 (MQ=255)
                                                                                                 gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                  >  2:180852/1‑150 (MQ=255)
                                                                                                 gACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCgg                                                  >  1:538568/1‑150 (MQ=255)
                                                                                                    aaCGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCaa                                               >  1:4775940/1‑150 (MQ=255)
                                                                                                    aaCGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCaa                                               >  2:1359688/1‑150 (MQ=255)
                                                                                                       gcacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCg                                            <  1:5728265/150‑1 (MQ=255)
                                                                                                        cacgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCgg                                           <  1:2278992/150‑1 (MQ=255)
                                                                                                         acgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGTGTACACGGTTCCGTCTTTTGACTGGATCAGCAGTTTGCCGTCAGCGGCTACCGGt                                          >  2:5548174/1‑150 (MQ=255)
                                                                                                         acgcTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTTCCGTCAGCGGCAACCggg                                          >  1:5027946/1‑149 (MQ=255)
                                                                                                            cTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCa                                       >  2:1454057/1‑150 (MQ=255)
                                                                                                            cTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCa                                       <  2:4182103/150‑1 (MQ=255)
                                                                                                                atTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCt                                   <  2:2819186/150‑1 (MQ=255)
                                                                                                                atTCGCGTCGTTGTTAAAAACAGGAAAACAGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCt                                   <  1:2333278/150‑1 (MQ=255)
                                                                                                                  tCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTgg                                 >  2:1241884/1‑150 (MQ=255)
                                                                                                                        cgtTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCg                           <  2:1126298/150‑1 (MQ=255)
                                                                                                                         gttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCgg                          >  2:753987/1‑150 (MQ=255)
                                                                                                                         gttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCgg                          >  2:3600870/1‑150 (MQ=255)
                                                                                                                          ttgttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTGTGCCGTCAGCGGCAACCGGTTCAGTCTGGAACCCGGa                         >  2:3851687/1‑150 (MQ=255)
                                                                                                                          ttgatAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTACAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGa                         <  1:2024741/150‑1 (MQ=255)
                                                                                                                             ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACt                      >  2:2393123/1‑150 (MQ=255)
                                                                                                                             ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACt                      <  2:4275347/150‑1 (MQ=255)
                                                                                                                             ttAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACt                      >  2:82163/1‑150 (MQ=255)
                                                                                                                              tAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTa                     >  2:369035/1‑150 (MQ=255)
                                                                                                                                 aaaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCa                  >  2:3457092/1‑150 (MQ=255)
                                                                                                                                 aaaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAAAGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCa                  >  1:1537491/1‑150 (MQ=255)
                                                                                                                                  aaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCaa                 <  1:3168497/150‑1 (MQ=255)
                                                                                                                                  aaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCaa                 <  2:5209045/150‑1 (MQ=255)
                                                                                                                                  aaCAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCaa                 <  2:4319829/150‑1 (MQ=255)
                                                                                                                                   ctttgAAAACGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAAc                >  1:3711433/5‑150 (MQ=255)
                                                                                                                                     aGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACtt              >  1:827831/1‑150 (MQ=255)
                                                                                                                                       gAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAAAGACAGAGACGATTAACGTGTAATATAGTACACGGTTACGTCATTTTCCTTGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGCACAATCAACattt            >  1:2338452/1‑150 (MQ=255)
                                                                                                                                        aaaaCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACttttt           <  1:1979480/150‑1 (MQ=255)
                                                                                                                                          aaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATTAACCTGTAATATAGTTCACGGTTCCGTCTTTTGACAGGAGCAGCAGGTTGCCGTCAGCGGCAACCCGTTCCGTCTGGAAACCGGAACTATCAACTTTTTGc         >  1:568535/1‑150 (MQ=255)
                                                                                                                                          aaCGGCTCCTGGACAGGGGCCGTTTTCAAAGTGAACGACAGAGACGATGAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCGGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAACCCGGACCGATCAACTTTTTGc         >  2:1027582/1‑150 (MQ=255)
                                                                                                                                          aaCGGCCCCTGTCCAGGAGCCGTTTTCAAAGTTAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTTCCTGGATCAGCATTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAAATATCAAAttttttg         >  1:5109787/1‑148 (MQ=255)
                                                                                                                                            cGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTg       <  1:4356547/150‑1 (MQ=255)
                                                                                                                                            cGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTg       <  1:5496543/150‑1 (MQ=255)
                                                                                                                                             ggCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTgg      <  2:4070076/150‑1 (MQ=255)
                                                                                                                                             ggCCCCTGGCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTgg      <  2:4768146/150‑1 (MQ=255)
                                                                                                                                               ccccTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGc    <  2:3305206/150‑1 (MQ=255)
                                                                                                                                                 ccTGTCCAGGAGCCGTTTTCAAAGTGAACGACCGAGACGATTAACGTGTAATAGAGGACACGGTTCCGTCTTGTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCaa  <  2:5627949/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
GTTAGACGGTTAAATAACGTGGATTTTCCTACGTTAGGGCGCCCGACAAGCGCGACCACAGGTACCATGTTTAAAGCCTCATTTTTATAAATCATCAGACAACGCACGCTATATTCGCGTCGTTGTTAAAAACAGGAAAACGGCCCCTGTCCAGGAGCCGTTTTCAAAGTGAACGACAGAGACGATTAACGTGTAATAGAGTACACGGTTCCGTCTTTTGCCTGGATCAGCAGTTTGCCGTCAGCGGCAACCGGTTCAGTCTGGAAACCGGAACTATCAACTTTTTGCTGGGCAA  >  NC_000913/2637289‑2637583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: